The aim here is to analyse your sequence in terms of a range of physicochemical parameters (hydropathy, flexibility, solvent- accessible surface area, etc.). The form allows comparison of three hydropathy scales, one of which is specific for trans- membrane proteins. Do the different scales give similar results? Does a change of window length significantly alter your result? (Note: when using this facility, you may have to specify a filename for use with an external Postscript viewer).

This form also allows you to view a Garnier-Osguthorpe- Robson secondary structure prediction profile (text output is also available to make interpretation of the result a little easier). For comparison, we suggest using nnpredict, which allows you to specify the tertiary structure class of your query sequence (but note that there are many other prediction packages available). How similar are the results from these methods, and how reliable do you think they are likely to be?

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