SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00056

Identifier
HSFDOMAIN  [View Relations]  [View Alignment]  
Accession
PR00056
No. of Motifs
3
Creation Date
19-JAN-1995  (UPDATE 07-JUN-1999)
Title
Heat shock factor (HSF) domain signature
Database References

PROSITE; PS00434 HSF_DOMAIN
PFAM; PF00447 HSF_DNA-bind
INTERPRO; IPR000232
Literature References
1. CLOS, J., WESTWOOD, J.T., BECKER, P.B., WILSON, S., LAMBERT, K.
AND WU, C.
Molecular cloning and expression of a hexameric Drosophila heat shock 
factor subject to negative regulation.
CELL 63 1085-1097 (1990).
 
2. RABINDRAN, S.K., GIORGI, G., CLOS, J. AND WU, C.
Molecular cloning and expression of a human heat shock factor, HSF1.
PROC.NATL.ACAD.SCI.U.S.A. 88 6906-6910 (1991).
 
3. SCHUETZ, T.J., GALLO, G.J., SHELDON, L., TEMPST, P. AND KINGSTON, R.E.
Isolation of a cDNA for HSF2: evidence for two heat shock factor genes
in humans.
PROC.NATL.ACAD.SCI.U.S.A. 88 6911-6915 (1991).

Documentation
Heat shock factor (HSF) is a transcriptional activator of heat shock genes
[1]: it binds specifically to heat shock promotor elements, which are
palindromic sequences rich with repetitive purine and pyrimidine motifs [1].
Under normal conditions, HSF is a homotrimeric cytoplasmic protein, but
heat shock activation results in relocalisation to the nucleus [2].
 
Each HSF monomer contains 1 C-terminal and 3 N-terminal leucine zipper
repeats [3]. Point mutations in these regions result in disruption of
cellular localisation, rendering the protein constitutively nuclear [2].
Two sequences flanking the N-terminal zippers fit the consensus of a bi-
partite nuclear localisation signal (NLS). Interaction between the N- and  
C-terminal zippers may result in a structure that masks the NLS sequences 
[3]: following activation of HSF, these may then be unmasked, resulting in 
relocalisation of the protein to the nucleus [3]. The DNA-binding component
of HSF lies to the N-terminus of the first NLS region, and is referred to
as the HSF domain.
 
HSFDOMAIN is a 3-element fingerprint that provides a signature for the 
heat shock factor domain. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions
around the centre of the domain, motifs 2 and 3 spanning the well-conserved
region encoded by PROSITE pattern HSF_DOMAIN (PS00434). Two iterations on
OWL25.1 were required to reach convergence, at which point a true set
containing 21 sequences was identified.
 
An update on SPTR37_9f identified a true set of 35 sequences.
Summary Information
35 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3353535
2000
123
True Positives
HSF1_ARATH    HSF1_CHICK    HSF1_HUMAN    HSF1_MOUSE    
HSF2_CHICK HSF2_HUMAN HSF2_LYCPE HSF2_MOUSE
HSF3_CHICK HSF3_LYCPE HSF8_LYCPE HSF_DROME
HSF_KLULA HSF_SCHPO HSF_XENLA HSF_YEAST
MGA1_YEAST O14283 O22230 O23186
O23259 O23615 O44943 O44944
O46359 O49403 O80982 Q40152
Q43457 Q43458 Q43700 Q96320
Q99472 SFL1_YEAST SKN7_YEAST
Sequence Titles
HSF1_ARATH  HEAT SHOCK FACTOR PROTEIN 1 (HSF 1) (HEAT SHOCK TRANSCRIPTION FACTOR 1) (HSTF 1) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS). 
HSF1_CHICK HEAT SHOCK FACTOR PROTEIN 1 (HSF 1) (HEAT SHOCK TRANSCRIPTION FACTOR 1) (HSTF 1) (HSF 3A) (HSTF 3A) - GALLUS GALLUS (CHICKEN).
HSF1_HUMAN HEAT SHOCK FACTOR PROTEIN 1 (HSF 1) (HEAT SHOCK TRANSCRIPTION FACTOR 1) (HSTF 1) - HOMO SAPIENS (HUMAN).
HSF1_MOUSE HEAT SHOCK FACTOR PROTEIN 1 (HSF 1) (HEAT SHOCK TRANSCRIPTION FACTOR 1) (HSTF 1) - MUS MUSCULUS (MOUSE).
HSF2_CHICK HEAT SHOCK FACTOR PROTEIN 2 (HSF 2) (HEAT SHOCK TRANSCRIPTION FACTOR 2) (HSTF 2) (HSF 3B) (HSTF 3B) - GALLUS GALLUS (CHICKEN).
HSF2_HUMAN HEAT SHOCK FACTOR PROTEIN 2 (HSF 2) (HEAT SHOCK TRANSCRIPTION FACTOR 2) (HSTF 2) - HOMO SAPIENS (HUMAN).
HSF2_LYCPE HEAT SHOCK FACTOR PROTEIN HSF24 (HEAT SHOCK TRANSCRIPTION FACTOR 24) (HSTF 24) (HEAT STRESS TRANSCRIPTION FACTOR) - LYCOPERSICON PERUVIANUM (PERUVIAN TOMATO).
HSF2_MOUSE HEAT SHOCK FACTOR PROTEIN 2 (HSF 2) (HEAT SHOCK TRANSCRIPTION FACTOR 2) (HSTF 2) - MUS MUSCULUS (MOUSE).
HSF3_CHICK HEAT SHOCK FACTOR PROTEIN 3 (HSF 3) (HEAT SHOCK TRANSCRIPTION FACTOR 3) (HSTF 3) (HSF 3C) (HSTF 3C) - GALLUS GALLUS (CHICKEN).
HSF3_LYCPE HEAT SHOCK FACTOR PROTEIN HSF30 (HEAT SHOCK TRANSCRIPTION FACTOR 30) (HSTF 30) (HEAT STRESS TRANSCRIPTION FACTOR) - LYCOPERSICON PERUVIANUM (PERUVIAN TOMATO).
HSF8_LYCPE HEAT SHOCK FACTOR PROTEIN HSF8 (HEAT SHOCK TRANSCRIPTION FACTOR 8) (HSTF 8) (HEAT STRESS TRANSCRIPTION FACTOR) - LYCOPERSICON PERUVIANUM (PERUVIAN TOMATO).
HSF_DROME HEAT SHOCK FACTOR PROTEIN (HSF) (HEAT SHOCK TRANSCRIPTION FACTOR) (HSTF) - DROSOPHILA MELANOGASTER (FRUIT FLY).
HSF_KLULA HEAT SHOCK FACTOR PROTEIN (HSF) (HEAT SHOCK TRANSCRIPTION FACTOR) (HSTF) - KLUYVEROMYCES LACTIS (YEAST).
HSF_SCHPO HEAT SHOCK FACTOR PROTEIN (HSF) (HEAT SHOCK TRANSCRIPTION FACTOR) (HSTF) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
HSF_XENLA HEAT SHOCK FACTOR PROTEIN (HSF) (HEAT SHOCK TRANSCRIPTION FACTOR) (HSTF) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
HSF_YEAST HEAT SHOCK FACTOR PROTEIN (HSF) (HEAT SHOCK TRANSCRIPTION FACTOR) (HSTF) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
MGA1_YEAST MGA1 PROTEIN - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
O14283 HYPOTHETICAL 61.7 KD PROTEIN C8C9.14 IN CHROMOSOME I - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O22230 PUTATIVE HEAT SHOCK TRANSCRIPTION FACTOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23186 HEAT SHOCK TRANSCRIPTION FACTOR HSF4 - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23259 HEAT SHOCK TRANSCRIPTION FACTOR HOMOLOG - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23615 HEAT SHOCK TRANSCRIPTION FACTOR HSF1 - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O44943 HEAT SHOCK TRANSCRIPTION FACTOR - SCHISTOSOMA MANSONI (BLOOD FLUKE).
O44944 HEAT SHOCK TRANSCRIPTION FACTOR - SCHISTOSOMA MANSONI (BLOOD FLUKE).
O46359 HEAT SHOCK TRANSCRIPTION FACTOR - SCHISTOSOMA MANSONI (BLOOD FLUKE).
O49403 HEAT SHOCK TRANSCRIPTION FACTOR - LIKE PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O80982 PUTATIVE HEAT SHOCK PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q40152 HEAT STRESS TRANSCRIPTION FACTOR 8 - LYCOPERSICON ESCULENTUM (TOMATO).
Q43457 HEAT SHOCK TRANSCRIPTION FACTOR 34 - GLYCINE MAX (SOYBEAN).
Q43458 HEAT SHOCK TRANSCRIPTION FACTOR 5 - GLYCINE MAX (SOYBEAN).
Q43700 HEAT SHOCK FACTOR - ZEA MAYS (MAIZE).
Q96320 HEAT SHOCK TRANSCRIPTION FACTOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q99472 HEAT SHOCK TRANSCRIPTION FACTOR 4 - HOMO SAPIENS (HUMAN).
SFL1_YEAST FLOCCULATION SUPPRESSION PROTEIN (SFL1 PROTEIN) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
SKN7_YEAST PUTATIVE TRANSCRIPTION FACTOR SKN7 (POS9 PROTEIN) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Scan History
OWL25_1    2  100  NSINGLE    
SPTR37_9f 2 38 NSINGLE
Initial Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
FLSKLWTLVEETHTNEFITWSQNG HSF2_HUMAN 10 10 -
FSNKLYNMVNDSSTDSLIRWSDRG HSF_SCHPO 53 53 -
FVNKLWSMVNDKSNEKFIHWSTSG HSF_KLULA 197 197 -
FLAKLWRLVDDADTNRLICWTKDG HSF_DROME 49 49 -
FIHKLYQILEDESLHDLIWWTPSG SFL1_YEAST 65 65 -

Motif 2 width=13
Element Seqn Id St Int Rpt
ILPKYFKHNNMAS HSF2_HUMAN 48 14 -
VLPRYFKHNNFSS HSF_SCHPO 91 14 -
VLPKYFKHSNFAS HSF_KLULA 235 14 -
LLPLNYKHNNMAS HSF_DROME 87 14 -
ALATYFKHTNITS SFL1_YEAST 102 13 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FVRQLNMYGFRKV HSF2_HUMAN 61 0 -
FVRQLNMYGFHKV HSF_SCHPO 104 0 -
FVRQLNMYGWHKV HSF_KLULA 248 0 -
FIRQLNMYGFHKI HSF_DROME 100 0 -
FVRQLNIYGFHKV SFL1_YEAST 115 0 -
Final Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
FLVKTYDMVDDPSTDKIVSWSPTN HSF8_LYCPE 42 42 -
FLVKTYDMVDDPSTDKIVSWSPTN Q40152 40 40 -
FLSKTYDMVEDPATDAIVSWSPTN HSF1_ARATH 53 53 -
FLSKTYDMVEDPATDAIVSWSPTN O23615 53 53 -
FLTKLWTLVEDPETDPLICWSPSG HSF1_CHICK 23 23 -
FLTKLWTLVSDPDTDALICWSPSG HSF1_HUMAN 18 18 -
FLTKLWTLVSDPDTDALICWSPSG HSF1_MOUSE 18 18 -
FLTKTYQLVEDQGTDQVISWGESG Q43457 10 10 -
FLAKLWTLVEDPDTDPLICWSPEG HSF_XENLA 15 15 -
FLLKTYQLVDDAATDDVISWNEIG HSF2_LYCPE 10 10 -
FLTKTYQLVDDPSTDHIVSWGEDD Q43458 25 25 -
FLAKLWALVEDPQSDDVICWSRNG HSF3_CHICK 19 19 -
FLSKTYEMVDDPATDAVVAWTPLG Q43700 10 10 -
FLSKTYQLVDDHSTDDVVSWNEEG O23186 15 15 -
FLVKTYEMVDDSSTDQIVSWSANN O23259 392 392 -
FLSKTYQLVDDHRTDDVVSWNEEG Q96320 15 15 -
FLGKLWALVGDPGTDHLIRWSPSG Q99472 21 21 -
FLSKLWTLVEETHTNEFITWSQNG HSF2_HUMAN 10 10 -
FLSKLWTLVEETHTNEFITWSQNG HSF2_MOUSE 10 10 -
FLTKLKLLVDDEETNELIYWDPHG O44943 16 16 -
FLTKLKLLVDDEETNELIYWDPHG O46359 16 16 -
FLTKLKLLVDDEETNELIYWDPHG O44944 16 16 -
FLTKTYEMVEDPATDTVVSWSNGR O80982 45 45 -
FSNKLYNMVNDSSTDSLIRWSDRG HSF_SCHPO 53 53 -
FLSKTYEMVEDSSTDQVISWSTTR HSF3_LYCPE 32 32 -
FLVKTYKVVEDPTTDGVISWNEYG O22230 41 41 -
FLSKLWALVGEAPSNQLITWSQNG HSF2_CHICK 24 24 -
FLTKTYEMVDDSSSDSIVSWSQSN O49403 16 16 -
FVNKLWSMVNDKSNEKFIHWSTSG HSF_KLULA 197 197 -
FLAKLWRLVDDADTNRLICWTKDG HSF_DROME 49 49 -
FVNKLWSMLNDDSNTKLIQWAEDG HSF_YEAST 175 175 -
FIHKLYQILEDESLHDLIWWTPSG SFL1_YEAST 65 65 -
FVRKLFNMLEEPEYRHILRWSDSG O14283 10 10 -
FVRKLFRILENNEYPDIVTWTENG SKN7_YEAST 87 87 -
FVHQLHAILLEPEVNKWIYWSPTD MGA1_YEAST 6 6 -

Motif 2 width=13
Element Seqn Id St Int Rpt
LLPKYFKHNNFSS HSF8_LYCPE 80 14 -
LLPKYFKHNNFSS Q40152 78 14 -
LLPKYFKHNNFSS HSF1_ARATH 91 14 -
LLPKYFKHNNFSS O23615 91 14 -
VLPKYFKHNNMAS HSF1_CHICK 61 14 -
VLPKYFKHNNMAS HSF1_HUMAN 56 14 -
VLPKYFKHNNMAS HSF1_MOUSE 56 14 -
LLPKYFKHNNFSS Q43457 48 14 -
VLPKYFKHNNMAS HSF_XENLA 53 14 -
LLPKYFKHNNFSS HSF2_LYCPE 48 14 -
LLPNYFKHNNFSS Q43458 63 14 -
LLPKYFKHNNISS HSF3_CHICK 57 14 -
LLPKYFKHNNFSS Q43700 48 14 -
LLPQYFKHNNFSS O23186 53 14 -
LLPTYFKHNNFSS O23259 430 14 -
LLPQYFKHNNFSS Q96320 53 14 -
VLPQYFKHSNMAS Q99472 59 14 -
ILPKYFKHNNMAS HSF2_HUMAN 48 14 -
ILPKYFKHNNMAS HSF2_MOUSE 48 14 -
LLPLYFKHNNLSS O44943 54 14 -
LLPLYFKHNNLSS O46359 54 14 -
LLPLYFKHNNLSS O44944 54 14 -
LLPRYFKHSNFSS O80982 83 14 -
VLPRYFKHNNFSS HSF_SCHPO 91 14 -
LLPRFFKHSNFSS HSF3_LYCPE 70 14 -
LLPTLFKHCNFSS O22230 79 14 -
ILPKYFKHNNMAS HSF2_CHICK 62 14 -
LLPRFFKHNNFSS O49403 54 14 -
VLPKYFKHSNFAS HSF_KLULA 235 14 -
LLPLNYKHNNMAS HSF_DROME 87 14 -
ILPKYFKHSNFAS HSF_YEAST 213 14 -
ALATYFKHTNITS SFL1_YEAST 102 13 -
ILPRHFKHSNFAS O14283 48 14 -
ILPNHFKHSNFAS SKN7_YEAST 125 14 -
VLKRYFKHGNVNS MGA1_YEAST 46 16 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FVRQLNTYGFRKV HSF8_LYCPE 93 0 -
FVRQLNTYGFRKV Q40152 91 0 -
FVRQLNTYGFRKV HSF1_ARATH 104 0 -
FVRQLNTYGFRKV O23615 104 0 -
FVRQLNMYGFRKV HSF1_CHICK 74 0 -
FVRQLNMYGFRKV HSF1_HUMAN 69 0 -
FVRQLNMYGFRKV HSF1_MOUSE 69 0 -
FVRQLNTYGFRKI Q43457 61 0 -
FVRQLNMYGFRKV HSF_XENLA 66 0 -
FVRQLNTYGFRKI HSF2_LYCPE 61 0 -
FVRQLNTYGFRKI Q43458 76 0 -
FIRQLNMYGFRKV HSF3_CHICK 70 0 -
FVRQLNTYGFKKI Q43700 61 0 -
FIRQLNTYGFRKT O23186 66 0 -
FIRQLNTYGFRKI O23259 443 0 -
FIRQLNTYGFRKT Q96320 66 0 -
FVRQLNMYGFRKV Q99472 72 0 -
FVRQLNMYGFRKV HSF2_HUMAN 61 0 -
FVRQLNMYGFRKV HSF2_MOUSE 61 0 -
FIRQLNMYGFRKI O44943 67 0 -
FIRQLNMYGFRKI O46359 67 0 -
FIRQLNMYGFRKI O44944 67 0 -
FIRQLNTYGFRKI O80982 96 0 -
FVRQLNMYGFHKV HSF_SCHPO 104 0 -
FIRQLNTYGFRKV HSF3_LYCPE 83 0 -
FVRQLNTYGFRKV O22230 92 0 -
FVRQLNMYGFRKV HSF2_CHICK 75 0 -
FIRQLNTYGFRKA O49403 67 0 -
FVRQLNMYGWHKV HSF_KLULA 248 0 -
FIRQLNMYGFHKI HSF_DROME 100 0 -
FVRQLNMYGWHKV HSF_YEAST 226 0 -
FVRQLNIYGFHKV SFL1_YEAST 115 0 -
FVRQLNKYDFHKV O14283 61 0 -
FVRQLNKYDFHKV SKN7_YEAST 138 0 -
FVRQLHMYGFHKL MGA1_YEAST 59 0 -