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PR01251

Identifier
AMPHIPHYSIN  [View Relations]  [View Alignment]  
Accession
PR01251
No. of Motifs
9
Creation Date
05-NOV-1999
Title
Amphiphysin signature
Database References
PRINTS; PR01252 AMPHIPHYSIN1
PRODOM; PD005419; PD003208; PD149887
INTERPRO; IPR003005
Literature References
1. WIGGE, P. AND MCMAHON, H.T.
The amphiphysin family of proteins and their role in endocytosis at the
synapse.
TRENDS NEUROSCIENCE 21 339-334 (1998).
 
2. TAKEI, K., SLEPNEV, V.I., HAUCKE, V. AND DE CAMILLI, P.
Functional partnership between amphiphysin and dynamin in clathrin-mediated
endocytosis.
NAT.CELL BIOL. 1 33-39 (1999).

Documentation
Amphiphysins are proteins that are thought to be involved in clathrin-
mediated endocytosis, actin function, and signalling pathways. They are
highly concentrated in nerve terminals, where they may act as linkers
between the clathrin coat and dynamin in the endocytosis of synaptic
vesicles. Such recycling of synaptic vesicles is necessary for
neurotransmission to continue, following neurotransmitter release.
Amphiphysin family members share a similar three-domain organisation: the
N-terminal domain contains six heptad repeats, which are predicted to form
a coiled-coiled structure, thought to be involved in the dimerisation of
amphiphysin molecules; the central region binds the heavy chain of clathrin
and the clathrin adaptor protein AP-2, through distinct sites; and the
C-terminal domain contains a Src-homology-3 (SH3) domain that binds the 
GTPase dynamin and the inositol-5'-phosphatase synaptojanin-1. The 
interactions mediated by both the central and C-terminal domains are
believed to be modulated by proten phosphorylation [1,2].
 
AMPHIPHYSIN is a 9-element fingerprint that provides a signature for the
amphiphysin family. The fingerprint was derived from an initial alignment
of 13 sequences: the motifs were drawn from conserved regions spanning
virtually the full alignment length - motifs 1-4 lie near the N-terminus
before the putative coiled-coil domain; motifs 5-7 encode portions of the
coiled-coil domain; motif 8 lies just prior to the C-terminal SH3 domain;
and motif 9 encodes ~1/5 of the SH3 domain. Two iterations on SPTR37_10f
were required to reach convergence, at which point a true set comprising 21
sequences was identified. A single partial match was also found, Q21004,
a sequence arising from the C.elegans Genome Project - this shows strong
similarity to motifs 1, 3 and 5, and appears to possess a C-terminal SH3 
domain, suggesting it may be a distant amphiphysin homologue.
Summary Information
  21 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9212121212121212121
8000000000
7000000000
6000000000
5000000000
4000000000
3101010000
2000000000
123456789
True Positives
AMPH_CHICK    AMPH_HUMAN    O00297        O00499        
O00545 O08539 O08838 O08839
O43538 O43867 O60552 O60553
O60554 O60555 O75514 O75515
O75516 O75517 O75518 Q62434
Q92944
True Positive Partials
Codes involving 3 elements
Q21004
Sequence Titles
AMPH_CHICK  AMPHIPHYSIN - GALLUS GALLUS (CHICKEN).        
AMPH_HUMAN AMPHIPHYSIN - HOMO SAPIENS (HUMAN).
O00297 AMPHIPHYSIN II - HOMO SAPIENS (HUMAN).
O00499 AMPHIPHYSIN II - HOMO SAPIENS (HUMAN).
O00545 AMPHIPHYSIN II - HOMO SAPIENS (HUMAN).
O08539 AMPHIPHYSIN-LIKE (BRAIN AMPHIPHYSIN 2) - MUS MUSCULUS (MOUSE).
O08838 AMPHIPHYSIN - RATTUS NORVEGICUS (RAT).
O08839 AMPHIPHYSIN - RATTUS NORVEGICUS (RAT).
O43538 AMPHIPHYSIN I - HOMO SAPIENS (HUMAN).
O43867 PUTATIVE TUMOR SUPPRESSOR - HOMO SAPIENS (HUMAN).
O60552 AMPHIPHYSIN IIB - HOMO SAPIENS (HUMAN).
O60553 AMPHIPHYSIN IIC1 - HOMO SAPIENS (HUMAN).
O60554 AMPHIPHYSIN IIC2 - HOMO SAPIENS (HUMAN).
O60555 AMPHIPHYSIN IID - HOMO SAPIENS (HUMAN).
O75514 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-10 - HOMO SAPIENS (HUMAN).
O75515 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-10-13 - HOMO SAPIENS (HUMAN).
O75516 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-13 - HOMO SAPIENS (HUMAN).
O75517 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1-10+12A - HOMO SAPIENS (HUMAN)
O75518 BOX-DEPENDENT MYC-INTERACTING PROTEIN ISOFORM BIN1+12A - HOMO SAPIENS (HUMAN).
Q62434 AMPHIPHYSIN-LIKE SH3 DOMAIN-CONTAINING PROTEIN SH3P9 - MUS MUSCULUS (MOUSE).
Q92944 BOX-DEPENDENT MYC-INTERACTING PROTEIN-1 - HOMO SAPIENS (HUMAN).

Q21004 F58G6.1 PROTEIN - CAENORHABDITIS ELEGANS.
Scan History
SPTR37_10f 2  100  NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
KVLQKLGKADETKDEQFE O00297 28 28 -
KVLQKLGKADETKDEQFE O60555 28 28 -
KVLQKLGKADETKDEQFE O60554 28 28 -
KVLQKLGKADETKDEQFE O60553 28 28 -
KVLQKLGKADETKDEQFE O60552 28 28 -
KVLQKLGKADETKDEQFE O00545 28 28 -
KVLQKLGKADETKDEQFE O00499 28 28 -
KVLQKLGKADETKDEQFE O08539 28 28 -
KVLQKLGKADETKDEQFE O08839 28 28 -
KVLQKLGKADETKDEQFE AMPH_HUMAN 23 23 -
KVLQKLGKADETKDEQFE O43538 23 23 -
KVLQKLGKADETKDEQFE O08838 23 23 -
KVLQKLGKADETKDEQFE AMPH_CHICK 23 23 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TEGTRLQKDLR O00297 56 10 -
TEGTRLQKDLR O60555 56 10 -
TEGTRLQKDLR O60554 56 10 -
TEGTRLQKDLR O60553 56 10 -
TEGTRLQKDLR O60552 56 10 -
TEGTRLQKDLR O00545 56 10 -
TEGTRLQKDLR O00499 56 10 -
TEGTRLQKDLR O08539 56 10 -
TEGTRLQKDLR O08839 56 10 -
AEGTRLQRELR AMPH_HUMAN 51 10 -
AEGTRLQRELR O43538 51 10 -
AEGTRLQRELR O08838 51 10 -
AEGSRLQRELR AMPH_CHICK 51 10 -

Motif 3 width=13
Element Seqn Id St Int Rpt
EVYEPDWPGRDEA O00297 88 21 -
EVYEPDWPGRDEA O60555 88 21 -
EVYEPDWPGRDEA O60554 88 21 -
EVYEPDWPGRDEA O60553 88 21 -
EVYEPDWPGRDEA O60552 88 21 -
EVYEPDWPGRDEA O00545 88 21 -
EVYEPDWPGRDEA O00499 88 21 -
EVYEPEWPGRDEA O08539 88 21 -
EVYEPEWPGRDEA O08839 88 21 -
EVYEPDWYGREDV AMPH_HUMAN 83 21 -
EVYEPDWYGREDV O43538 83 21 -
EVYEPDWYGREDV O08838 83 21 -
EVYEPDWYGREDV AMPH_CHICK 83 21 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LLTMDTYLGQFPDIK O00297 123 22 -
LLTMDTYLGQFPDIK O60555 123 22 -
LLTMDTYLGQFPDIK O60554 123 22 -
LLTMDTYLGQFPDIK O60553 123 22 -
LLTMDTYLGQFPDIK O60552 123 22 -
LLTMDTYLGQFPDIK O00545 123 22 -
LLTMDTYLGQFPDIK O00499 123 22 -
LLTMDTYLGQFPDIK O08539 123 22 -
LLTMDTYLGQFPDIK O08839 123 22 -
LLTLDTYLGQFPDIK AMPH_HUMAN 118 22 -
LLTLDTYLGQFPDIK O43538 118 22 -
LLTLDTYLGQFPDIK O08838 118 22 -
LLTLDTYLGQFPDIK AMPH_CHICK 118 22 -

Motif 5 width=12
Element Seqn Id St Int Rpt
RKLVDYDSARHH O00297 145 7 -
RKLVDYDSARHH O60555 145 7 -
RKLVDYDSARHH O60554 145 7 -
RKLVDYDSARHH O60553 145 7 -
RKLVDYDSARHH O60552 145 7 -
RKLVDYDSARHH O00545 145 7 -
RKLVDYDSARHH O00499 145 7 -
RKLVDYDSARHH O08539 145 7 -
RKLVDYDSARHH O08839 145 7 -
RKLVDYDSARHH AMPH_HUMAN 140 7 -
RKLVDYDSARHH O43538 140 7 -
RKLVDYDSARHH O08838 140 7 -
RKLVDYDSARHH AMPH_CHICK 140 7 -

Motif 6 width=10
Element Seqn Id St Int Rpt
KAQKVFEEMN O00297 211 54 -
KAQKVFEEMN O60555 211 54 -
KAQKVFEEMN O60554 180 23 -
KAQKVFEEMN O60553 211 54 -
KAQKVFEEMN O60552 180 23 -
KAQKVFEEMN O00545 180 23 -
KAQKVFEEMN O00499 211 54 -
KAQKVFEEMN O08539 211 54 -
KAQKVFEEMN O08839 211 54 -
KAQKVFEEFN AMPH_HUMAN 175 23 -
KAQKVFEEFN O43538 175 23 -
KAQKVFEEFN O08838 175 23 -
KAQKVFEEFN AMPH_CHICK 175 23 -

Motif 7 width=11
Element Seqn Id St Int Rpt
DLQEELPSLWN O00297 222 1 -
DLQEELPSLWN O60555 222 1 -
DLQEELPSLWN O60554 191 1 -
DLQEELPSLWN O60553 222 1 -
DLQEELPSLWN O60552 191 1 -
DLQEELPSLWN O00545 191 1 -
DLQEELPSLWN O00499 222 1 -
DLQEELPSLWN O08539 222 1 -
DLQEELPSLWN O08839 222 1 -
DLQEELPSLWS AMPH_HUMAN 186 1 -
DLQEELPSLWS O43538 186 1 -
DLQEELPSLWS O08838 186 1 -
DLQEELPSLWS AMPH_CHICK 186 1 -

Motif 8 width=10
Element Seqn Id St Int Rpt
DLPPGFMFKV O00297 516 283 -
DLPPGFMFKV O60555 473 240 -
DLPPGFMFKV O60554 398 196 -
DLPPGFMFKV O60553 429 196 -
DLPPGFMFKV O60552 441 239 -
DLPPGFMFKV O00545 405 203 -
DLPPGFMFKV O00499 516 283 -
DLPPGFMFKV O08539 511 278 -
DLPPGFMFKV O08839 511 278 -
ELPPGFLYKV AMPH_HUMAN 618 421 -
ELPPGFLYKV O43538 576 379 -
ELPPGFLYKV O08838 606 409 -
DMPPGFLFKV AMPH_CHICK 605 408 -

Motif 9 width=15
Element Seqn Id St Int Rpt
DELQLKAGDVVLVIP O00297 537 11 -
DELQLKAGDVVLVIP O60555 494 11 -
DELQLKAGDVVLVIP O60554 419 11 -
DELQLKAGDVVLVIP O60553 450 11 -
DELQLKAGDVVLVIP O60552 462 11 -
DELQLKAGDVVLVIP O00545 426 11 -
DELQLKAGDVVLVIP O00499 537 11 -
DELQLKAGDVVLVIP O08539 532 11 -
DELQLKAGDVVLVIP O08839 532 11 -
DELTLQRGDVVLVVP AMPH_HUMAN 639 11 -
DELTLQRGDVVLVVP O43538 597 11 -
DELTLQRGDVVLVVP O08838 627 11 -
DELNLKRGDIVLVIP AMPH_CHICK 626 11 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
KVLQKLGKADETKDEQFE O00297 28 28 -
KVLQKLGKADETKDEQFE Q92944 28 28 -
KVLQKLGKADETKDEQFE O75518 28 28 -
KVLQKLGKADETKDEQFE O75517 28 28 -
KVLQKLGKADETKDEQFE O75516 28 28 -
KVLQKLGKADETKDEQFE O75515 28 28 -
KVLQKLGKADETKDEQFE O75514 28 28 -
KVLQKLGKADETKDEQFE O60555 28 28 -
KVLQKLGKADETKDEQFE O60554 28 28 -
KVLQKLGKADETKDEQFE O60553 28 28 -
KVLQKLGKADETKDEQFE O60552 28 28 -
KVLQKLGKADETKDEQFE O43867 28 28 -
KVLQKLGKADETKDEQFE O00545 28 28 -
KVLQKLGKADETKDEQFE O00499 28 28 -
KVLQKLGKADETKDEQFE O08539 28 28 -
KVLQKLGKADETKDEQFE O08839 28 28 -
KVLQKLGKADETKDEQFE Q62434 28 28 -
KVLQKLGKADETKDEQFE AMPH_HUMAN 23 23 -
KVLQKLGKADETKDEQFE O43538 23 23 -
KVLQKLGKADETKDEQFE O08838 23 23 -
KVLQKLGKADETKDEQFE AMPH_CHICK 23 23 -

Motif 2 width=11
Element Seqn Id St Int Rpt
TEGTRLQKDLR O00297 56 10 -
TEGTRLQKDLR Q92944 56 10 -
TEGTRLQKDLR O75518 56 10 -
TEGTRLQKDLR O75517 56 10 -
TEGTRLQKDLR O75516 56 10 -
TEGTRLQKDLR O75515 56 10 -
TEGTRLQKDLR O75514 56 10 -
TEGTRLQKDLR O60555 56 10 -
TEGTRLQKDLR O60554 56 10 -
TEGTRLQKDLR O60553 56 10 -
TEGTRLQKDLR O60552 56 10 -
TEGTRLQKDLR O43867 56 10 -
TEGTRLQKDLR O00545 56 10 -
TEGTRLQKDLR O00499 56 10 -
TEGTRLQKDLR O08539 56 10 -
TEGTRLQKDLR O08839 56 10 -
TEGTRLQKDLR Q62434 56 10 -
AEGTRLQRELR AMPH_HUMAN 51 10 -
AEGTRLQRELR O43538 51 10 -
AEGTRLQRELR O08838 51 10 -
AEGSRLQRELR AMPH_CHICK 51 10 -

Motif 3 width=13
Element Seqn Id St Int Rpt
EVYEPDWPGRDEA O00297 88 21 -
EVYEPDWPGRDEA Q92944 88 21 -
EVYEPDWPGRDEA O75518 88 21 -
EVYEPDWPGRDEA O75517 88 21 -
EVYEPDWPGRDEA O75516 88 21 -
EVYEPDWPGRDEA O75515 88 21 -
EVYEPDWPGRDEA O75514 88 21 -
EVYEPDWPGRDEA O60555 88 21 -
EVYEPDWPGRDEA O60554 88 21 -
EVYEPDWPGRDEA O60553 88 21 -
EVYEPDWPGRDEA O60552 88 21 -
EVYEPDWPGRDEA O43867 88 21 -
EVYEPDWPGRDEA O00545 88 21 -
EVYEPDWPGRDEA O00499 88 21 -
EVYEPEWPGRDEA O08539 88 21 -
EVYEPEWPGRDEA O08839 88 21 -
EVYEPEWPGRDEA Q62434 88 21 -
EVYEPDWYGREDV AMPH_HUMAN 83 21 -
EVYEPDWYGREDV O43538 83 21 -
EVYEPDWYGREDV O08838 83 21 -
EVYEPDWYGREDV AMPH_CHICK 83 21 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LLTMDTYLGQFPDIK O00297 123 22 -
LLTMDTYLGQFPDIK Q92944 123 22 -
LLTMDTYLGQFPDIK O75518 123 22 -
LLTMDTYLGQFPDIK O75517 123 22 -
LLTMDTYLGQFPDIK O75516 123 22 -
LLTMDTYLGQFPDIK O75515 123 22 -
LLTMDTYLGQFPDIK O75514 123 22 -
LLTMDTYLGQFPDIK O60555 123 22 -
LLTMDTYLGQFPDIK O60554 123 22 -
LLTMDTYLGQFPDIK O60553 123 22 -
LLTMDTYLGQFPDIK O60552 123 22 -
LLTMDTYLGQFPDIK O43867 123 22 -
LLTMDTYLGQFPDIK O00545 123 22 -
LLTMDTYLGQFPDIK O00499 123 22 -
LLTMDTYLGQFPDIK O08539 123 22 -
LLTMDTYLGQFPDIK O08839 123 22 -
LLTMDTYLGQFPDIK Q62434 123 22 -
LLTLDTYLGQFPDIK AMPH_HUMAN 118 22 -
LLTLDTYLGQFPDIK O43538 118 22 -
LLTLDTYLGQFPDIK O08838 118 22 -
LLTLDTYLGQFPDIK AMPH_CHICK 118 22 -

Motif 5 width=12
Element Seqn Id St Int Rpt
RKLVDYDSARHH O00297 145 7 -
RKLVDYDSARHH Q92944 145 7 -
RKLVDYDSARHH O75518 145 7 -
RKLVDYDSARHH O75517 145 7 -
RKLVDYDSARHH O75516 145 7 -
RKLVDYDSARHH O75515 145 7 -
RKLVDYDSARHH O75514 145 7 -
RKLVDYDSARHH O60555 145 7 -
RKLVDYDSARHH O60554 145 7 -
RKLVDYDSARHH O60553 145 7 -
RKLVDYDSARHH O60552 145 7 -
RKLVDYDSARHH O43867 145 7 -
RKLVDYDSARHH O00545 145 7 -
RKLVDYDSARHH O00499 145 7 -
RKLVDYDSARHH O08539 145 7 -
RKLVDYDSARHH O08839 145 7 -
RKLVDYDSARHH Q62434 145 7 -
RKLVDYDSARHH AMPH_HUMAN 140 7 -
RKLVDYDSARHH O43538 140 7 -
RKLVDYDSARHH O08838 140 7 -
RKLVDYDSARHH AMPH_CHICK 140 7 -

Motif 6 width=10
Element Seqn Id St Int Rpt
KAQKVFEEMN O00297 211 54 -
KAQKVFEEMN Q92944 180 23 -
KAQKVFEEMN O75518 180 23 -
KAQKVFEEMN O75517 180 23 -
KAQKVFEEMN O75516 180 23 -
KAQKVFEEMN O75515 180 23 -
KAQKVFEEMN O75514 180 23 -
KAQKVFEEMN O60555 211 54 -
KAQKVFEEMN O60554 180 23 -
KAQKVFEEMN O60553 211 54 -
KAQKVFEEMN O60552 180 23 -
KAQKVFEEMN O43867 180 23 -
KAQKVFEEMN O00545 180 23 -
KAQKVFEEMN O00499 211 54 -
KAQKVFEEMN O08539 211 54 -
KAQKVFEEMN O08839 211 54 -
KAQKVFEEMN Q62434 180 23 -
KAQKVFEEFN AMPH_HUMAN 175 23 -
KAQKVFEEFN O43538 175 23 -
KAQKVFEEFN O08838 175 23 -
KAQKVFEEFN AMPH_CHICK 175 23 -

Motif 7 width=11
Element Seqn Id St Int Rpt
DLQEELPSLWN O00297 222 1 -
DLQEELPSLWN Q92944 191 1 -
DLQEELPSLWN O75518 191 1 -
DLQEELPSLWN O75517 191 1 -
DLQEELPSLWN O75516 191 1 -
DLQEELPSLWN O75515 191 1 -
DLQEELPSLWN O75514 191 1 -
DLQEELPSLWN O60555 222 1 -
DLQEELPSLWN O60554 191 1 -
DLQEELPSLWN O60553 222 1 -
DLQEELPSLWN O60552 191 1 -
DLQEELPSLWN O43867 191 1 -
DLQEELPSLWN O00545 191 1 -
DLQEELPSLWN O00499 222 1 -
DLQEELPSLWN O08539 222 1 -
DLQEELPSLWN O08839 222 1 -
DLQEELPSLWN Q62434 191 1 -
DLQEELPSLWS AMPH_HUMAN 186 1 -
DLQEELPSLWS O43538 186 1 -
DLQEELPSLWS O08838 186 1 -
DLQEELPSLWS AMPH_CHICK 186 1 -

Motif 8 width=10
Element Seqn Id St Int Rpt
DLPPGFMFKV O00297 516 283 -
DLPPGFMFKV Q92944 377 175 -
DLPPGFMFKV O75518 420 218 -
DLPPGFMFKV O75517 405 203 -
DLPPGFMFKV O75516 347 145 -
DLPPGFMFKV O75515 332 130 -
DLPPGFMFKV O75514 362 160 -
DLPPGFMFKV O60555 473 240 -
DLPPGFMFKV O60554 398 196 -
DLPPGFMFKV O60553 429 196 -
DLPPGFMFKV O60552 441 239 -
DLPPGFMFKV O43867 420 218 -
DLPPGFMFKV O00545 405 203 -
DLPPGFMFKV O00499 516 283 -
DLPPGFMFKV O08539 511 278 -
DLPPGFMFKV O08839 511 278 -
DLPPGFMFKV Q62434 357 155 -
ELPPGFLYKV AMPH_HUMAN 618 421 -
ELPPGFLYKV O43538 576 379 -
ELPPGFLYKV O08838 606 409 -
DMPPGFLFKV AMPH_CHICK 605 408 -

Motif 9 width=15
Element Seqn Id St Int Rpt
DELQLKAGDVVLVIP O00297 537 11 -
DELQLKAGDVVLVIP Q92944 398 11 -
DELQLKAGDVVLVIP O75518 441 11 -
DELQLKAGDVVLVIP O75517 426 11 -
DELQLKAGDVVLVIP O75516 368 11 -
DELQLKAGDVVLVIP O75515 353 11 -
DELQLKAGDVVLVIP O75514 383 11 -
DELQLKAGDVVLVIP O60555 494 11 -
DELQLKAGDVVLVIP O60554 419 11 -
DELQLKAGDVVLVIP O60553 450 11 -
DELQLKAGDVVLVIP O60552 462 11 -
DELQLKAGDVVLVIP O43867 441 11 -
DELQLKAGDVVLVIP O00545 426 11 -
DELQLKAGDVVLVIP O00499 537 11 -
DELQLKAGDVVLVIP O08539 532 11 -
DELQLKAGDVVLVIP O08839 532 11 -
DELQLKAGDVVLVIP Q62434 378 11 -
DELTLQRGDVVLVVP AMPH_HUMAN 639 11 -
DELTLQRGDVVLVVP O43538 597 11 -
DELTLQRGDVVLVVP O08838 627 11 -
DELNLKRGDIVLVIP AMPH_CHICK 626 11 -