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PR00043

Identifier
LEUZIPPRJUN  [View Relations]  [View Alignment]  
Accession
PR00043
No. of Motifs
5
Creation Date
21-APR-1993  (UPDATE 07-JUN-1999)
Title
Jun transcription factor signature
Database References

PROSITE; PS00036 FOS_JUN_BASIC; PS00029 LEUCINE_ZIPPER
INTERPRO; IPR002112
PDB; 1DGC; 1YSA
SCOP; 1DGC;
CATH; 1YSA
Literature References
1. BOHMANN, D., BOS, T.J., ADMON, A., NISHIMURA, T., VOGT, P.K. AND 
TIJAN, R.
Human proto-oncogene c-jun encodes a DNA-binding protein with structural
and functional properties of transcription factor AP-1.
SCIENCE 238 1386-1392 (1987).
 
2. HATTORI, K., ANGEL, P., LEBEAU, M.M. AND KARIN, M.
Structure and chromosomal localization of the functional intronless human
jun proto-oncogene.
PROC.NATL.ACAD.SCI.U.S.A. 85 9148-9152 (1988).
 
3. RYDER, K., LAU, L.F. AND NATHANS, D.
A gene activated by growth factors is related to the oncogene v-jun.
PROC.NATL.ACAD.SCI.U.S.A. 85 1487-1491 (1988).

Documentation
AP-1 (activator protein 1), also known as c-jun, is the cellular homologue 
of the the avian sarcoma virus oncogene v-jun, and as such can be referred 
to as a proto-oncogene. Implicit in the growth regulatory functions of all 
proto-oncogenes is the potential to induce abnormal cell growth and cancer 
as a result of alterations in gene expression [1]. This may be a quali-
tative or quantitative alteration, the viral oncogenes activating this 
potential by transducing a truncated or mutated form of the protein 
product, or by increasing transcription of the proto-oncogene by the 
integration of a viral promoter and enhancer sequence in its vicinity.
 
The jun gene encodes a protein that is structurally and funtionally 
identical to the transcription factor AP-1, which is itself identical to 
yeast transcriptional activator GCN4 [2]. Such similarities imply that the
jun product acts in the nucleus as a transcription factor, the high 
structural identity with GCN4 suggesting that the 2 proteins may recognise 
similar DNA sequences (GCN4 binds to the 5'-TGA(C/G)TCA-3' consensus
sequence). It has been proposed that the highly basic region in the
C-terminus of the protein may be the DNA-binding domain [3]. The gene 
products of jun and fos can come together as a dimer to bind DNA via
a leucine zipper, which occurs just after the DNA-binding site. 
 
The `leucine zipper' is a structure that is believed to mediate the
function of several eukaryotic gene regulatory proteins. The zipper
consists of a periodic repetition of leucine residues at every seventh
position, and regions containing them appear to span 8 turns of alpha-
helix. The leucine side chains that extend from one helix interact with
those from a similar helix, hence facilitating dimerisation in the form
of a coiled-coil. Leucine zippers are present in many gene regulatory
proteins, including the CREB proteins, Jun/AP1 transcription factors,
fos oncogene and fos-related proteins, C-myc, L-myc and N-myc oncogenes,
and so on.
 
LEUZIPPRJUN is a 5-element fingerprint that provides a signature for the 
leucine zipper and DNA-binding domains characteristic of jun/AP1 tran-
scription factors. The fingerprint was derived from an initial alignment 
of 6 sequences: motifs 1 and 2 span the highly basic DNA-binding domain,
while motifs 3 and 4 encode the leucine zipper region (cf. PROSITE patterns
FOS_JUN_BASIC (PS00036) and LEUCINE_ZIPPER (PS00029)). Two iterations on
OWL19.1 were required to reach convergence, at which point a true set
comprising 17 sequences was identified. Several partial matches were also
found: these include the GCN4 protein, which lacks the portion of sequence
bearing the last motif, and various CREB proteins.
 
An update on SPTR37_9f identified a true set of 17 sequences.
Summary Information
17 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
51717171717
400000
300000
200000
12345
True Positives
AP1_CHICK     AP1_COTJA     AP1_DROME     AP1_HUMAN     
AP1_MOUSE AP1_PIG AP1_RAT AP1_SERCA
JUNB_CYPCA JUNB_HUMAN JUNB_MOUSE JUNB_RAT
JUND_CHICK JUND_HUMAN JUND_MOUSE JUND_RAT
TJUN_AVIS1
Sequence Titles
AP1_CHICK   TRANSCRIPTION FACTOR AP-1 (PROTO-ONCOGENE C-JUN) - GALLUS GALLUS (CHICKEN). 
AP1_COTJA TRANSCRIPTION FACTOR AP-1 (PROTO-ONCOGENE C-JUN) - COTURNIX COTURNIX JAPONICA (JAPANESE QUAIL).
AP1_DROME TRANSCRIPTION FACTOR JUN (DJUN) (DJRA) (JUN-RELATED ANTIGEN) (AP-1) - DROSOPHILA MELANOGASTER (FRUIT FLY).
AP1_HUMAN TRANSCRIPTION FACTOR AP-1 (PROTO-ONCOGENE C-JUN) (P39) (G0S7) - HOMO SAPIENS (HUMAN).
AP1_MOUSE TRANSCRIPTION FACTOR AP-1 (PROTO-ONCOGENE JUN A) (AH119) - MUS MUSCULUS (MOUSE).
AP1_PIG TRANSCRIPTION FACTOR AP-1 (PROTO-ONCOGENE C-JUN) - SUS SCROFA (PIG).
AP1_RAT TRANSCRIPTION FACTOR AP-1 (PROTO-ONCOGENE C-JUN) - RATTUS NORVEGICUS (RAT).
AP1_SERCA TRANSCRIPTION FACTOR AP-1 (PROTO-ONCOGENE C-JUN) - SERINUS CANARIA (CANARY).
JUNB_CYPCA TRANSCRIPTION FACTOR JUN-B - CYPRINUS CARPIO (COMMON CARP).
JUNB_HUMAN TRANSCRIPTION FACTOR JUN-B (G0S3) - HOMO SAPIENS (HUMAN).
JUNB_MOUSE TRANSCRIPTION FACTOR JUN-B - MUS MUSCULUS (MOUSE).
JUNB_RAT TRANSCRIPTION FACTOR JUN-B - RATTUS NORVEGICUS (RAT).
JUND_CHICK TRANSCRIPTION FACTOR JUN-D - GALLUS GALLUS (CHICKEN).
JUND_HUMAN TRANSCRIPTION FACTOR JUN-D - HOMO SAPIENS (HUMAN).
JUND_MOUSE TRANSCRIPTION FACTOR JUN-D - MUS MUSCULUS (MOUSE).
JUND_RAT TRANSCRIPTION FACTOR JUN-D - RATTUS NORVEGICUS (RAT).
TJUN_AVIS1 TRANSFORMING PROTEIN JUN - AVIAN SARCOMA VIRUS (STRAIN 17).
Scan History
OWL19_1    2  100  NSINGLE    
OWL26_0 1 150 NSINGLE
SPTR37_9f 2 18 NSINGLE
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
TPPLSPIDMESQERIKAERKR TAP1_CHICK 218 218 -
TPPLSPIDMESQERIKAERKR TAP1_COTJA 221 221 -
TPPLSPIDMESQERIKAERKR TAP1_HUMAN 239 239 -
TPPVSPINMEDQERIKVERKR TAPB_HUMAN 255 255 -
TPPVSPINMEDQERIKVERKR TAPB_MOUSE 252 252 -
SPPLSPIDMDTQERIKAERKR TAPD_MOUSE 249 249 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RNRIAASKCRKRKLERI TAP1_CHICK 240 1 -
RNRIAASKCRKRKLERI TAP1_COTJA 243 1 -
RNRIAASKCRKRKLERI TAP1_HUMAN 261 1 -
RNRLAATKCRKRKLERI TAPB_HUMAN 277 1 -
RNRLAATKCRKRKLERI TAPB_MOUSE 274 1 -
RNRIAASKCRKRKLERI TAPD_MOUSE 271 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
RLEEKVKTLKAQN TAP1_CHICK 258 1 -
RLEEKVKTLKAQN TAP1_COTJA 261 1 -
RLEEKVKTLKAQN TAP1_HUMAN 279 1 -
RLEDKVKTLKAEN TAPB_HUMAN 295 1 -
RLEDKVKTLKAEN TAPB_MOUSE 292 1 -
RLEEKVKTLKSQN TAPD_MOUSE 289 1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LASTANMLREQVAQL TAP1_CHICK 273 2 -
LASTANMLREQVAQL TAP1_COTJA 276 2 -
LASTANMLREQVAQL TAP1_HUMAN 294 2 -
LSSTAGLLREQVAQL TAPB_HUMAN 310 2 -
LSSAAGLLREQVAQL TAPB_MOUSE 307 2 -
LASTASLLREQVAQL TAPD_MOUSE 304 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
LKQKVMNHVNSGCQLM TAP1_CHICK 287 -1 -
LKQKVMNHVNSGCQLM TAP1_COTJA 290 -1 -
LKQKVMNHVNSGCQLM TAP1_HUMAN 308 -1 -
LKQKVMTHVSNGCQLL TAPB_HUMAN 324 -1 -
LKQKVMTHVSNGCQLL TAPB_MOUSE 321 -1 -
LKQKVLSHVNSGCQLL TAPD_MOUSE 318 -1 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
TPPLSPIDMESQERIKAERKR AP1_CHICK 218 218 -
TPPLSPIDMESQERIKAERKR AP1_SERCA 222 222 -
TPPLSPIDMESQERIKAERKR AP1_RAT 242 242 -
TPPLSPIDMESQERIKAERKR AP1_PIG 239 239 -
TPPLSPIDMESQERIKAERKR AP1_MOUSE 242 242 -
TPPLSPIDMESQERIKAERKR AP1_HUMAN 239 239 -
TPPLSPIDMESQERIKAERKR AP1_COTJA 221 221 -
TPPLFPIDMESQERIKAERKR TJUN_AVIS1 204 204 -
SPPLSPIDMDTQERIKAERKR JUND_HUMAN 255 255 -
SPPLSPIDMDTQERIKAERKR JUND_RAT 249 249 -
SPPLSPIDMDTQERIKAERKR JUND_MOUSE 249 249 -
SPPLSPIDMDTQERIKAERKR JUND_CHICK 229 229 -
TPPVSPINMEDQERIKVERKR JUNB_HUMAN 255 255 -
TPPVSPINMEDQERIKVERKR JUNB_MOUSE 252 252 -
TPPVSPINMEDQERIKVERKR JUNB_RAT 252 252 -
SPPTSPIDMENQERIKAERKR JUNB_CYPCA 216 216 -
SPTVNPIDMEAQEKIKLERKR AP1_DROME 199 199 -

Motif 2 width=17
Element Seqn Id St Int Rpt
RNRIAASKCRKRKLERI AP1_CHICK 240 1 -
RNRIAASKCRKRKLERI AP1_SERCA 244 1 -
RNRIAASKCRKRKLERI AP1_RAT 264 1 -
RNRIAASKCRKRKLERI AP1_PIG 261 1 -
RNRIAASKCRKRKLERI AP1_MOUSE 264 1 -
RNRIAASKCRKRKLERI AP1_HUMAN 261 1 -
RNRIAASKCRKRKLERI AP1_COTJA 243 1 -
RNRIAASKSRKRKLERI TJUN_AVIS1 226 1 -
RNRIAASKCRKRKLERI JUND_HUMAN 277 1 -
RNRIAASKCRKRKLERI JUND_RAT 271 1 -
RNRIAASKCRKRKLERI JUND_MOUSE 271 1 -
RNRIAASKCRKRKLERI JUND_CHICK 251 1 -
RNRLAATKCRKRKLERI JUNB_HUMAN 277 1 -
RNRLAATKCRKRKLERI JUNB_MOUSE 274 1 -
RNRLAATKCRKRKLERI JUNB_RAT 274 1 -
RNRLAATKCRKRKLERI JUNB_CYPCA 238 1 -
RNRVAASKCRKRKLERI AP1_DROME 221 1 -

Motif 3 width=13
Element Seqn Id St Int Rpt
RLEEKVKTLKAQN AP1_CHICK 258 1 -
RLEEKVKTLKAQN AP1_SERCA 262 1 -
RLEEKVKTLKAQN AP1_RAT 282 1 -
RLEEKVKTLKAQN AP1_PIG 279 1 -
RLEEKVKTLKAQN AP1_MOUSE 282 1 -
RLEEKVKTLKAQN AP1_HUMAN 279 1 -
RLEEKVKTLKAQN AP1_COTJA 261 1 -
RLEEKVKTLKAQN TJUN_AVIS1 244 1 -
RLEEKVKTLKSQN JUND_HUMAN 295 1 -
RLEEKVKTLKSQN JUND_RAT 289 1 -
RLEEKVKTLKSQN JUND_MOUSE 289 1 -
RLEEKVKSLKSQN JUND_CHICK 269 1 -
RLEDKVKTLKAEN JUNB_HUMAN 295 1 -
RLEDKVKTLKAEN JUNB_MOUSE 292 1 -
RLEDKVKTLKAEN JUNB_RAT 292 1 -
RLEEKVKVLKNDN JUNB_CYPCA 256 1 -
KLEDRVKVLKGEN AP1_DROME 239 1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LASTANMLREQVAQL AP1_CHICK 273 2 -
LASTANMLREQVAQL AP1_SERCA 277 2 -
LASTANMLREQVAQL AP1_RAT 297 2 -
LASTANMLREQVAQL AP1_PIG 294 2 -
LASTANMLREQVAQL AP1_MOUSE 297 2 -
LASTANMLREQVAQL AP1_HUMAN 294 2 -
LASTANMLREQVAQL AP1_COTJA 276 2 -
LASTANMLREQVAQL TJUN_AVIS1 259 2 -
LASTASLLREQVAQL JUND_HUMAN 310 2 -
LASTASLLREQVAQL JUND_RAT 304 2 -
LASTASLLREQVAQL JUND_MOUSE 304 2 -
LASTASLLREQVAQL JUND_CHICK 284 2 -
LSSTAGLLREQVAQL JUNB_HUMAN 310 2 -
LSSAAGLLREQVAQL JUNB_MOUSE 307 2 -
LSSAAGLLREQVAQL JUNB_RAT 307 2 -
LSNTASVLRDQVAQL JUNB_CYPCA 271 2 -
LASIVKNLKDHVAHV AP1_DROME 254 2 -

Motif 5 width=16
Element Seqn Id St Int Rpt
LKQKVMNHVNSGCQLM AP1_CHICK 287 -1 -
LKQKVMNHVNSGCQLM AP1_SERCA 291 -1 -
LKQKVMNHVNSGCQLM AP1_RAT 311 -1 -
LKQKVMNHVNSGCQLM AP1_PIG 308 -1 -
LKQKVMNHVNSGCQLM AP1_MOUSE 311 -1 -
LKQKVMNHVNSGCQLM AP1_HUMAN 308 -1 -
LKQKVMNHVNSGCQLM AP1_COTJA 290 -1 -
LKQKVMNHVNSGCQLM TJUN_AVIS1 273 -1 -
LKQKVLSHVNSGCQLL JUND_HUMAN 324 -1 -
LKQKVLSHVNSGCQLL JUND_RAT 318 -1 -
LKQKVLSHVNSGCQLL JUND_MOUSE 318 -1 -
LKQKVLSHVNSGCQLL JUND_CHICK 298 -1 -
LKQKVMTHVSNGCQLL JUNB_HUMAN 324 -1 -
LKQKVMTHVSNGCQLL JUNB_MOUSE 321 -1 -
LKQKVMTHVSNGCQLL JUNB_RAT 321 -1 -
LKQKVLRHMNSGCQLM JUNB_CYPCA 285 -1 -
VKQQVMEHIAAGCTVP AP1_DROME 268 -1 -