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PR00004

Identifier
ANAPHYLATOXN  [View Relations]  [View Alignment]  
Accession
PR00004
No. of Motifs
3
Creation Date
21-OCT-1994  (UPDATE 13-JUN-1999)
Title
Anaphylatoxin domain signature
Database References

PROSITE; PS01177 ANAPHYLATOXIN_1; PS01178 ANAPHYLATOXIN_2
INTERPRO; IPR001840
PDB; 1C5A
SCOP; 1C5A
CATH; 1C5A
Literature References
1. FRITZINGER, D.C., PETRELLA, E.C., CONNELLY, M.B., BREDEHORST, R.
AND VOGEL, C.W.
Primary structure of cobra complement component C3.
J.IMMUNOL. 149 3554-3562 (1992).
 
2. OGATA, R.T., ROSA, P.A. AND ZEPF, N.E.
Sequence of the gene for murine complement component C4.
J.BIOL.CHEM. 264 16565-16572 (1989).
 
3. GENNARO, R., SIMONIC, T., NEGRI, A., MOTTOLA, C., SECCHI, C., RONCHI, S.
AND ROMEO, D.
C5a fragment of bovine complement. Purification, bioassays, amino-acid 
sequence and other structural studies.
EUR.J.BIOCHEMISTRY 155 77-86 (1986).

Documentation
Complement components C3, C4 and C5 are large glycoproteins that have
important functions in the immune response and host defence [1]. They have 
a wide variety of biological activities and are proteolytically activated 
by cleavage at a specific site, forming a- and b-fragments [2]. A-fragments 
form distinct structural domains of approximately 76 amino acids, coded for
by a single exon within the complement protein gene. The C3a, C4a and C5a 
components are referred to as anaphylatoxins [2,3]: they cause smooth muscle
contraction, histamine release from mast cells, and enhanced vascular 
permeability [3]; they also mediate chemotaxis, inflammation, and generation
of cytotoxic oxygen radicals [3]. The proteins are highly hydrophilic, with 
a mainly alpha-helical structure held together by 3 disulphide bridges [3].
 
ANAPHYLATOXN is a 3-element fingerprint that provides a signature for 
complement C3a, C4a and C5a anaphylatoxin domains. The fingerprint was
derived from an initial alignment of 6 sequences: the motifs encode 
conserved regions containing 6 cysteines involved in disulphide bond
formation. Two iterations on OWL24.0 were required to reach convergence,
at which point a true set comprising 28 sequences was identified.
 
An update on SPTR37_9f identified a true set of 21 sequences.
Summary Information
21 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3212121
2000
123
True Positives
CO3A_PIG      CO3_CAVPO     CO3_HUMAN     CO3_MOUSE     
CO3_NAJNA CO3_RAT CO4A_RAT CO4_HUMAN
CO4_MOUSE CO5A_BOVIN CO5A_PIG CO5A_RAT
CO5_HUMAN CO5_MOUSE O70346 O73905
Q13160 Q13906 Q90633 Q91132
Q91741
Sequence Titles
CO3A_PIG    COMPLEMENT C3A ANAPHYLATOXIN - SUS SCROFA (PIG). 
CO3_CAVPO COMPLEMENT C3 PRECURSOR [CONTAINS: C3A ANAPHYLATOXIN] - CAVIA PORCELLUS (GUINEA PIG).
CO3_HUMAN COMPLEMENT C3 PRECURSOR [CONTAINS: C3A ANAPHYLATOXIN] - HOMO SAPIENS (HUMAN).
CO3_MOUSE COMPLEMENT C3 PRECURSOR (HSE-MSF) [CONTAINS: C3A ANAPHYLATOXIN] - MUS MUSCULUS (MOUSE).
CO3_NAJNA COMPLEMENT C3 PRECURSOR [CONTAINS: C3A ANAPHYLATOXIN] - NAJA NAJA (INDIAN COBRA).
CO3_RAT COMPLEMENT C3 PRECURSOR [CONTAINS: C3A ANAPHYLATOXIN] - RATTUS NORVEGICUS (RAT).
CO4A_RAT COMPLEMENT C4A ANAPHYLATOXIN - RATTUS NORVEGICUS (RAT).
CO4_HUMAN COMPLEMENT C4 PRECURSOR [CONTAINS: C4A ANAPHYLATOXIN] - HOMO SAPIENS (HUMAN).
CO4_MOUSE COMPLEMENT C4 PRECURSOR [CONTAINS: C4A ANAPHYLATOXIN] - MUS MUSCULUS (MOUSE).
CO5A_BOVIN COMPLEMENT C5A ANAPHYLATOXIN - BOS TAURUS (BOVINE).
CO5A_PIG COMPLEMENT C5A ANAPHYLATOXIN - SUS SCROFA (PIG).
CO5A_RAT COMPLEMENT C5A ANAPHYLATOXIN - RATTUS NORVEGICUS (RAT).
CO5_HUMAN COMPLEMENT C5 PRECURSOR [CONTAINS: C5A ANAPHYLATOXIN] - HOMO SAPIENS (HUMAN).
CO5_MOUSE COMPLEMENT C5 PRECURSOR [CONTAINS: C5A ANAPHYLATOXIN] - MUS MUSCULUS (MOUSE).
O70346 COMPLEMENT C4 - MUS MUSCULUS (MOUSE).
O73905 COMPLEMENT C4 - GALLUS GALLUS (CHICKEN).
Q13160 COMPLEMENT C4B PRECURSOR - HOMO SAPIENS (HUMAN).
Q13906 COMPLEMENT COMPONENT C4A - HOMO SAPIENS (HUMAN).
Q90633 COMPLEMENT C3 PRECURSOR - GALLUS GALLUS (CHICKEN).
Q91132 COBRA VENOM FACTOR PRECURSOR - NAJA NAJA (INDIAN COBRA).
Q91741 FOURTH COMPONENT OF COMPLEMENT - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
Scan History
OWL24_0    2  100  NSINGLE    
OWL28_0 1 200 NSINGLE
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
VPKKCCYDGA CO5A_RAT 20 20 -
ELRRCCEHGM CO3A_PIG 18 18 -
VAKRCCQDGL CO4A_BOVIN 19 19 -
DTKRCCQDGM CO4A_RAT 19 19 -
MLKKCCYDGA CO5A_PIG 17 17 -
WVKKCCYDGA CO5A_BOVIN 17 17 -

Motif 2 width=12
Element Seqn Id St Int Rpt
TCEERAARIKIG CO5A_PIG 33 6 -
TCEQRAARVQQP CO4A_BOVIN 36 7 -
TCEQRAARVPQP CO4A_RAT 36 7 -
TCEERAARIAIG CO5A_BOVIN 33 6 -
TCEQRVARVTIG CO5A_RAT 36 6 -
SCQRRAQFIHQG CO3A_PIG 35 7 -

Motif 3 width=12
Element Seqn Id St Int Rpt
ACVKAFLNCCEY CO3A_PIG 48 1 -
ACREPFLSCCKF CO4A_RAT 48 0 -
KCVKAFKDCCYI CO5A_PIG 46 1 -
ACREPFLSCCQF CO4A_BOVIN 48 0 -
ECIKAFKSCCAI CO5A_BOVIN 46 1 -
HCIRAFKECCTI CO5A_RAT 49 1 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
DAKRCCQDGM CO4_MOUSE 696 696 -
DAKRCCQDGM O70346 696 696 -
DTKRCCQDGM CO4A_RAT 19 19 -
TAKRCCQDGV CO4_HUMAN 698 698 -
TAKRCCQDGV Q13160 698 698 -
TAKRCCQDGV Q13906 698 698 -
ELRRCCEDGM CO3_CAVPO 694 694 -
GLRKCCEDGM CO3_NAJNA 679 679 -
MLKKCCYDGA CO5A_PIG 17 17 -
VVKKCCYDGA CO5_HUMAN 694 694 -
WVKKCCYDGA CO5A_BOVIN 17 17 -
GLRKCCEDGM CO3_RAT 689 689 -
GLRKCCEDGM CO3_MOUSE 689 689 -
DLRKCCEDVM Q91132 673 673 -
VPKKCCYDGA CO5A_RAT 20 20 -
ELRRCCEHGM CO3A_PIG 18 18 -
ELRKCCEDGM CO3_HUMAN 689 689 -
NLRKCCEDGI Q90633 682 682 -
VPKKCCYDGA CO5_MOUSE 698 698 -
ELQRCCQHGM Q91741 679 679 -
VMWRCCRDGA O73905 573 573 -

Motif 2 width=12
Element Seqn Id St Int Rpt
TCEQRAARVPQQ CO4_MOUSE 713 7 -
TCEQRAARVPQQ O70346 713 7 -
TCEQRAARVPQP CO4A_RAT 36 7 -
SCEQRAARVQQP CO4_HUMAN 715 7 -
SCEQRAARVQQP Q13160 715 7 -
SCEQRAARVQQL Q13906 715 7 -
SCQRRARYVSLG CO3_CAVPO 711 7 -
TCEKRAKYIQEG CO3_NAJNA 696 7 -
TCEERAARIKIG CO5A_PIG 33 6 -
TCEQRAARISLG CO5_HUMAN 710 6 -
TCEERAARIAIG CO5A_BOVIN 33 6 -
SCQRRARLITQG CO3_RAT 706 7 -
SCQRRARLITQG CO3_MOUSE 706 7 -
TCEKRAKYIQEG Q91132 690 7 -
TCEQRVARVTIG CO5A_RAT 36 6 -
SCQRRAQFIHQG CO3A_PIG 35 7 -
SCQRRTRFISLG CO3_HUMAN 706 7 -
SCEKRATYVLDA Q90633 699 7 -
TCEERVARVTIG CO5_MOUSE 714 6 -
VCTKRAARVPDP Q91741 698 9 -
TCQQRGQRVTTA O73905 590 7 -

Motif 3 width=12
Element Seqn Id St Int Rpt
ACREPFLSCCKF CO4_MOUSE 725 0 -
ACREPFLSCCKF O70346 725 0 -
ACREPFLSCCKF CO4A_RAT 48 0 -
DCREPFLSCCQF CO4_HUMAN 727 0 -
DCREPFLSCCQF Q13160 727 0 -
DCREPFLSCCQF Q13906 727 0 -
ACVKAFLDCCTY CO3_CAVPO 724 1 -
ACKAAFLECCHY CO3_NAJNA 709 1 -
KCVKAFKDCCYI CO5A_PIG 46 1 -
RCIKAFTECCVV CO5_HUMAN 723 1 -
ECIKAFKSCCAI CO5A_BOVIN 46 1 -
SCLKAFMDCCNY CO3_RAT 719 1 -
NCIKAFIDCCNH CO3_MOUSE 719 1 -
ACKAAFLECCRY Q91132 703 1 -
HCIRAFKECCTI CO5A_RAT 49 1 -
ACVKAFLNCCEY CO3A_PIG 48 1 -
ACKKVFLDCCNY CO3_HUMAN 719 1 -
SCTEAFLSCCLY Q90633 712 1 -
LCIRAFNECCTI CO5_MOUSE 727 1 -
TCRKAFLDCCEY Q91741 710 0 -
GCRDAFLQCCEV O73905 603 1 -