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PR01487

Identifier
LUXSPROTEIN  [View Relations]  [View Alignment]  
Accession
PR01487
No. of Motifs
5
Creation Date
16-MAR-2001
Title
Bacterial autoinducer-2 (AI-2) production protein LuxS signature
Database References
Literature References
1. BASSLER, B.L. 
How bacteria talk to each other: regulation of gene expression by quorum 
sensing.
CURR.OPIN.MICROBIOL. 2 582-587 (1999).
 
2. SURETTE, M.G., MILLER, M.B. AND BASSLER, B.L.
Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio 
harveyi: A new family of genes responsible for autoinducer production.
PROC.NATL.ACAD.SCI.U.S.A. 96 1639-1644 (1999).
 
3. JOYCE, E.A., BASSLER, B.L. AND WRIGHT, A.
Evidence for a signaling system in Helicobacter pylori: Detection of a 
luxS-encoded autoinducer.
J.BACTERIOL. 182 3638-3643 (2000).
 
4. DAY, W.A. AND MAURELLI, A.T.
Shigella flexneri LuxS quorum-sensing system modulates virB expression but 
is not essential for virulence.
INFECT.IMMUN. 69 15-23 (2001).

Documentation
Bacterial species have many methods of controlling gene expression and cell
growth. Regulation of gene expression in response to changes in cell density
is termed quorum sensing [1,2]. Quorum-sensing bacteria produce, release and
respond to hormone-like molecules (autoinducers) that accumulate in the 
external environment as the cell population grows. Once a threshold of these
molecules is reached, a signal transduction cascade is triggered that 
ultimately leads to behavioural changes in the bacterium [2]. In the marine
bacterium Vibrio harveyi, two parallel quorum-sensing systems are found
(designated AI-1 and AI-2), each comprising a sensor-autoinducer pair [2].
 
It has been suggested that quorum-sensing might play a part in the virulence
of some pathogens. Regulation of these genes differs between virulent and
benign strains [2]. In the former, production of autoinducers ceases when
the organism reaches stationary growth phase [3], and may allow pathogenesis
to take place [4]. The AI-2 system appears to be the most important in these
processes. A two-component regulon controls the system, the gene LuxS being
responsible for synthesis of the autoinducers. Organisms as diverse as 
Helicobacter pylori, Vibrio harveyi, Clostridium perfringens and Shigella 
flexneri [4] contain this gene.
 
LUXSPROTEIN is a 5-element fingerprint that provides a signature for the
bacterial autoinducer-2 (AI-2) production protein LuxS. The fingerprint was 
derived from an initial alignment of 3 sequences: the motifs were drawn from
conserved regions spanning the full alignment length (~120 amino acids). Two
iterations on SPTR37_10f were required to reach convergence, at which point 
a true set comprising 9 sequences was identified. A single partial match was
also found, LUXS_BORBU, a Borrelia burgdoferi LuxS protein that matches
motifs 4 and 5.
Summary Information
   9 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
599999
400000
300000
200011
12345
True Positives
LUXS_BACSU    LUXS_CLOPE    LUXS_ECOLI    LUXS_HAEIN    
LUXS_HELPJ LUXS_HELPY LUXS_VIBHA Q9PN97
Q9RRU8
True Positive Partials
Codes involving 2 elements
LUXS_BORBU
Sequence Titles
LUXS_BACSU  AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Bacillus subtilis. 
LUXS_CLOPE AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Clostridium perfringens.
LUXS_ECOLI AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Escherichia coli.
LUXS_HAEIN AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Haemophilus influenzae.
LUXS_HELPJ AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Helicobacter pylori J99 (Campylobacter pylori J99).
LUXS_HELPY AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Helicobacter pylori (Campylobacter pylori).
LUXS_VIBHA AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Vibrio harveyi.
Q9PN97 HYPOTHETICAL PROTEIN CJ1198 - Campylobacter jejuni.
Q9RRU8 CONSERVED HYPOTHETICAL PROTEIN - Deinococcus radiodurans.

LUXS_BORBU AUTOINDUCER-2 PRODUCTION PROTEIN LUXS (AI-2 SYNTHESIS PROTEIN) - Borrelia burgdorferi (Lyme disease spirochete).
Scan History
SPTR39_14f 2  75   NSINGLE    
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
DSFTVDHTRMNAPAVRVAK LUXS_VIBHA 4 4 -
DSFKVDHTKMNAPAVRIAK LUXS_HAEIN 4 4 -
ESFELDHTKVKAPYVRKAG LUXS_CLOPE 5 5 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PKGDTITVFDLRFTAPNKD LUXS_VIBHA 27 4 -
PKGDNITVFDLRFCIPNKE LUXS_HAEIN 27 4 -
PKGDIVSKFDLRFVQPNKE LUXS_CLOPE 28 4 -

Motif 3 width=19
Element Seqn Id St Int Rpt
SEKGIHTLEHLYAGFMRNH LUXS_VIBHA 48 2 -
SPKGIHTLEHLFAGFMRDH LUXS_HAEIN 48 2 -
SDKGMHTLEHFLAGFMREK LUXS_CLOPE 49 2 -

Motif 4 width=19
Element Seqn Id St Int Rpt
DISPMGCRTGFYMSLIGTP LUXS_VIBHA 76 9 -
DISPMGCRTGFYMSLIGTP LUXS_HAEIN 76 9 -
DISPMGCKTGFYLTSFGDI LUXS_CLOPE 73 5 -

Motif 5 width=20
Element Seqn Id St Int Rpt
PELNEYQCGTAAMHSLDEAK LUXS_VIBHA 120 25 -
PELNIYQCGSYTEHSLEDAH LUXS_HAEIN 120 25 -
PAANELQCGSAKLHSLELAK LUXS_CLOPE 114 22 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
DSFTVDHTRMNAPAVRVAK LUXS_VIBHA 4 4 -
DSFKVDHTKMPAPAVRLAK Q9PN97 5 5 -
DSFKVDHTKMNAPAVRIAK LUXS_HAEIN 4 4 -
DSFTVDHTRMEAPAVRVAK LUXS_ECOLI 4 4 -
ESFELDHTKVKAPYVRKAG LUXS_CLOPE 5 5 -
ESFNLDHTKVKAPYVRVAD LUXS_HELPY 9 9 -
ESFNLDHTKVKAPYVRIAD LUXS_HELPJ 6 6 -
ESFDLDHTKVKAPYVRLAG Q9RRU8 8 8 -
ESFELDHNAVVAPYVRHCG LUXS_BACSU 5 5 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PKGDTITVFDLRFTAPNKD LUXS_VIBHA 27 4 -
PKGDDISVFDLRFCIPNKD Q9PN97 28 4 -
PKGDNITVFDLRFCIPNKE LUXS_HAEIN 27 4 -
PHGDAITVFDLRFCVPNKE LUXS_ECOLI 27 4 -
PKGDIVSKFDLRFVQPNKE LUXS_CLOPE 28 4 -
VNGDLIVKYDVRFKQPNQD LUXS_HELPY 32 4 -
VNGDLIVKYDVRFKQPNRD LUXS_HELPJ 29 4 -
PKGDQISKYDLRFLQPNQG Q9RRU8 31 4 -
GTDGVVNKFDIRFCQPNKQ LUXS_BACSU 28 4 -

Motif 3 width=19
Element Seqn Id St Int Rpt
SEKGIHTLEHLYAGFMRNH LUXS_VIBHA 48 2 -
SEKGTHTLEHLFAGFMRDH Q9PN97 49 2 -
SPKGIHTLEHLFAGFMRDH LUXS_HAEIN 48 2 -
PERGIHTLEHLFAGFMRNH LUXS_ECOLI 48 2 -
SDKGMHTLEHFLAGFMREK LUXS_CLOPE 49 2 -
DMPSLHSLEHLVAEIIRNH LUXS_HELPY 53 2 -
DMPSLHSLEHLVAEIIRNH LUXS_HELPJ 50 2 -
DPAAIHTLEHLLAGYMRDH Q9RRU8 52 2 -
KPDTIHTLEHLLAFTIRSH LUXS_BACSU 49 2 -

Motif 4 width=19
Element Seqn Id St Int Rpt
DISPMGCRTGFYMSLIGTP LUXS_VIBHA 76 9 -
DISPMGCRTGFYMSLIGTP Q9PN97 77 9 -
DISPMGCRTGFYMSLIGTP LUXS_HAEIN 76 9 -
DISPMGCRTGFYMSLIGTP LUXS_ECOLI 76 9 -
DISPMGCKTGFYLTSFGDI LUXS_CLOPE 73 5 -
DWSPMGCQTGFYLTVLNHD LUXS_HELPY 77 5 -
DWSPMGCQTGFYLTVLNHD LUXS_HELPJ 74 5 -
DVSPMGCRTGMYMAVIGEP Q9RRU8 76 5 -
DISPMGCQTGYYLVVSGEP LUXS_BACSU 78 10 -

Motif 5 width=20
Element Seqn Id St Int Rpt
PELNEYQCGTAAMHSLDEAK LUXS_VIBHA 120 25 -
PELNIYQCGTCAMHSLDEAK Q9PN97 121 25 -
PELNIYQCGSYTEHSLEDAH LUXS_HAEIN 120 25 -
PELNVYQCGTYQMHSLQEAQ LUXS_ECOLI 120 25 -
PAANELQCGSAKLHSLELAK LUXS_CLOPE 114 22 -
PASNEKQCGWAANHTLEGAK LUXS_HELPY 118 22 -
PASNEKQCGWAANHTLEGAQ LUXS_HELPJ 115 22 -
PGVSELECGNYRDHDLAAAR Q9RRU8 118 23 -
PAANEKQCGQAKLHDLEGAK LUXS_BACSU 119 22 -