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PR01397

Identifier
DHBDHDRGNASE  [View Relations]  [View Alignment]  
Accession
PR01397
No. of Motifs
6
Creation Date
05-SEP-2000
Title
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature
Database References
PRINTS; PR00080 SDRFAMILY
PROSITE; PS00061 ADH_SHORT
PFAM; PF00106 adh_short
INTERPRO; IPR002198; IPR002347
UMBBD; e0411; e0362; e0005
Literature References
1. PAYNE, S.M.
Detection, isolation, and characterisation of siderophores.
METHODS ENZYMOL. 235 329-344 (1994).
 
2. NAHLIK, M.S., BRICKMAN, T.J., OZENBERGER, B.A. AND MCINTOSH, M.A.
Nucleotide sequence and transcriptional organisation of the Escherichia 
coli enterobactin biosynthesis cistrons entB and entA.
J.BACTERIOL. 171 784-790 (1989).
 
3. WYCKOFF, E.E., STOEBNER, J.A., REED, K.E. AND PAYNE, S.M. 
Cloning of a Vibrio cholera vibriobactin gene cluster: identification of
genes required for early steps in siderophore synthesis.
J.BACTERIOL. 179 7055-7062 (1997).
 
4. LIU, J., DUNCAN, K. AND WALSH, C.T.
Nucleotide sequence of a cluster of Escherichia coli enterobactin 
biosynthesis genes: identification of entA and purification of its product
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase.
J.BACTERIOL. 171 791-798 (1989).

Documentation
Iron is essential for growth in both bacteria and mammals. Controlling the
amount of free iron in solution is often used as a tactic by hosts to limit
invasion of pathogenic microbes; binding iron tightly within protein
molecules can accomplish this. Such iron-protein complexes include haem in
blood, lactoferrin in tears/saliva and transferrin in blood plasma. Some
bacteria express surface receptors to capture eukaryotic iron-binding
compounds, while others have evolved siderophores to scavenge iron from
iron-binding host proteins [1].
 
The absence of free iron molecules in the surrounding environment triggers 
transcription of gene clusters that encode both siderophore-synthesis 
enzymes, and receptors that recognise iron-bound siderophores [2]. Classic
examples are the enterobactin/enterochelin clusters found in Escherichia
coli and Salmonella spp., although similar moieties in other pathogens have 
been identified. The enzymic machinery that produces vibrionectin in Vibrio
cholera is such a homologue [3].
 
EntA, a 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase enzyme, is involved
in the third stage of enterobactin biosynthesis and converts isochorismate 
to 2,3-dihydroxybenzoic acid (DHBA). Deletion studies involving EntA- 
mutants have shown that it is essential for virulence [4].
 
DHBDHDRGNASE is a 6-element fingerprint that provides a signature for  
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenases. The fingerprint was
derived from an initial alignment of 3 sequences: the motifs were drawn
from conserved regions spanning the full alignment length. A single
iteration on SPTR37_10f was required to reach convergence, no further 
sequences being identified beyond the starting set. 
Summary Information
3 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6333333
5000000
4000000
3000000
2000000
123456
True Positives
DHBA_BACSU    ENTA_ECOLI    VIBA_VIBCH    
Sequence Titles
DHBA_BACSU  2,3-DIHYDRO-2,3-DIHYDROXYBENZOATE DEHYDROGENASE (EC 1.3.1.28) (COLD SHOCK PROTEIN CSI14) - BACILLUS SUBTILIS. 
ENTA_ECOLI 2,3-DIHYDRO-2,3-DIHYDROXYBENZOATE DEHYDROGENASE (EC 1.3.1.28) - ESCHERICHIA COLI.
VIBA_VIBCH VIBRIOBACTIN-SPECIFIC 2,3-DIHYDRO-2,3-DIHYDROXYBENZOATE DEHYDROGENASE (EC 1.3.1.28) - VIBRIO CHOLERAE.
Scan History
SPTR37_10f 2  10   NSINGLE    
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
GAAQGIGEAVARTLASQG DHBA_BACSU 15 15 -
GAGKGIGYATALAFVEAG ENTA_ECOLI 12 12 -
GSARGIGFSVLEHLLQAG VIBA_VIBCH 15 15 -

Motif 2 width=18
Element Seqn Id St Int Rpt
DILVNVAGVLRPGLIHSL DHBA_BACSU 87 54 -
DALVNAAGILRMGATDQL ENTA_ECOLI 74 44 -
DHLVCCAGILHVAPLHEM VIBA_VIBCH 88 55 -

Motif 3 width=21
Element Seqn Id St Int Rpt
EEWEATFSVNSTGVFNASRSV DHBA_BACSU 107 2 -
EDWQQTFAVNVGGAFNLFQQT ENTA_ECOLI 94 2 -
EQVSSIFTVNAFGVLACMQGV VIBA_VIBCH 108 2 -

Motif 4 width=24
Element Seqn Id St Int Rpt
GLELAEYNIRCNIVSPGSTETDMQ DHBA_BACSU 172 44 -
GLELAGSGVRCNVVSPGSTDTDMQ ENTA_ECOLI 159 44 -
GMELAPYGIRCNLVSPGSTRTAMQ VIBA_VIBCH 173 44 -

Motif 5 width=20
Element Seqn Id St Int Rpt
GSLETFKTGIPLKKLAKPSD DHBA_BACSU 211 15 -
GFGEQFKLGIPLGKIARPQE ENTA_ECOLI 198 15 -
GDAAQFRLGIPLNKIAEPAD VIBA_VIBCH 212 15 -

Motif 6 width=20
Element Seqn Id St Int Rpt
FLVSGQAGHITMHNLCVDGG DHBA_BACSU 237 6 -
FLASDLASHITLQDIVVDGG ENTA_ECOLI 224 6 -
FLLSDNAGHITLHDLRVDGG VIBA_VIBCH 238 6 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
GAAQGIGEAVARTLASQG DHBA_BACSU 15 15 -
GAGKGIGYATALAFVEAG ENTA_ECOLI 12 12 -
GSARGIGFSVLEHLLQAG VIBA_VIBCH 15 15 -

Motif 2 width=18
Element Seqn Id St Int Rpt
DILVNVAGVLRPGLIHSL DHBA_BACSU 87 54 -
DALVNAAGILRMGATDQL ENTA_ECOLI 74 44 -
DHLVCCAGILHVAPLHEM VIBA_VIBCH 88 55 -

Motif 3 width=21
Element Seqn Id St Int Rpt
EEWEATFSVNSTGVFNASRSV DHBA_BACSU 107 2 -
EDWQQTFAVNVGGAFNLFQQT ENTA_ECOLI 94 2 -
EQVSSIFTVNAFGVLACMQGV VIBA_VIBCH 108 2 -

Motif 4 width=24
Element Seqn Id St Int Rpt
GLELAEYNIRCNIVSPGSTETDMQ DHBA_BACSU 172 44 -
GLELAGSGVRCNVVSPGSTDTDMQ ENTA_ECOLI 159 44 -
GMELAPYGIRCNLVSPGSTRTAMQ VIBA_VIBCH 173 44 -

Motif 5 width=20
Element Seqn Id St Int Rpt
GSLETFKTGIPLKKLAKPSD DHBA_BACSU 211 15 -
GFGEQFKLGIPLGKIARPQE ENTA_ECOLI 198 15 -
GDAAQFRLGIPLNKIAEPAD VIBA_VIBCH 212 15 -

Motif 6 width=20
Element Seqn Id St Int Rpt
FLVSGQAGHITMHNLCVDGG DHBA_BACSU 237 6 -
FLASDLASHITLQDIVVDGG ENTA_ECOLI 224 6 -
FLLSDNAGHITLHDLRVDGG VIBA_VIBCH 238 6 -