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PR01283

Identifier
ECDYSTEROIDR  [View Relations]  [View Alignment]  
Accession
PR01283
No. of Motifs
8
Creation Date
13-FEB-2000
Title
Ecdysteroid receptor (1H nuclear receptor) signature
Database References
PRINTS; PR00398 STRDHORMONER
Literature References
1. NUCLEAR RECEPTORS NOMENCLATURE COMMITTEE
A unified nomenclature system for the nuclear receptor superfamily.
CELL 97 161-163 (1999).
 
2. NISHIKAWA, J-I., KITAURA, M., IMAGAWA, M. AND NISHIHARA, T.
Vitamin D receptor contains multiple dimerisation interfaces that
are functionally different.
NUCLEIC ACIDS RES. 23(4) 606-611 (1995).
 
3. DE VOS, P., SCHMITT, J., VERHOEVEN, G. AND STUNNENBERG, G.
Human androgen receptor expressed in HeLa cells activates transcription
in vitro.
NUCLEIC ACIDS RES. 22(7) 1161-1166 (1994).
 
4. TALBOT, W.S., SWYRYD, E.A. AND HOGNESS, D.S.
Drosophila tissues with different metamorphic responses to ecdysone express
different ecdysone receptor isoforms.
CELL 73 1323-1337 (1993). 
 
5. CHO, W.L., KAPITSKAYA, M.Z. AND RAIKHEL, A.S.
Mosquito ecdysteroid receptor: analysis of the cDNA and expression during
vitellogenesis.
INSECT BIOCHEM.MOL.BIOL. 25 19-27 (1995). 

Documentation
Steroid or nuclear hormone receptors (NRs) constitute an important super-
family of transcription regulators that are involved in widely diverse 
physiological functions, including control of embryonic development, cell
differentiation and homeostasis [1]. Members of the superfamily include the
steroid hormone receptors and receptors for thyroid hormone, retinoids, 
1,25-dihydroxy-vitamin D3 and a variety of other ligands. The proteins 
function as dimeric molecules in nuclei to regulate the transcription of 
target genes in a ligand-responsive manner [2,3]. In addition to C-terminal
ligand-binding domains, these nuclear receptors contain a highly-conserved,
N-terminal zinc-finger that mediates specific binding to target DNA 
sequences, termed ligand-responsive elements. In the absence of ligand,
steroid hormone receptors are thought to be weakly associated with nuclear
components; hormone binding greatly increases receptor affinity.
 
NRs are extremely important in medical research, a large number of them
being implicated in diseases such as cancer, diabetes, hormone resistance
syndromes, etc. [1]. While several NRs act as ligand-inducible transcription
factors, many do not yet have a defined ligand and are accordingly termed 
"orphan" receptors. During the last decade, more than 300 NRs have been
described, many of which are orphans, which cannot easily be named due to 
current nomenclature confusions in the literature. However, a new system 
has recently been introduced in an attempt to rationalise the increasingly 
complex set of names used to describe superfamily members [1].
 
In Drosophila melanogaster, the steroid hormone ecdysone triggers larval-to-
adult metamorphosis, a process in which the hormone induces imaginal tissues
to generate adult structures, and larval tissues to degenerate [4]. The
ecdysone receptor (EcR) binds DNA with high specificity at ecdysone response
elements. EcR is nuclear and is found in larval wing discs, pupal wings and
in prothoracic glands. 
 
In the mosquito Aedes aegypti, 20-hydroxyecdysone plays an important role
in the regulation of egg maturation [5]. There are three EcR transcripts
(of 4.2kb, 6kb and 11kb) in adult mosquitoes; 4.2kb mRNA is predominantly
expressed in female mosquitoes during vitellogenesis [5]. In both the fat
body and ovaries of the female mosquito, the level of EcR mRNA is high at
the previtellogenic period and after the onset of vitellogenesis [5]. 
 
ECDYSTEROIDR is an 8-element fingerprint that provides a signature for
ecdysone receptors. The fingerprint was derived from an initial alignment 
of 5 sequences: the motifs were drawn from conserved regions spanning 
virtually the full alignment length, focusing on those sections that
characterise the ecdysone receptors but distinguish them from the rest of 
the steroid hormone receptor superfamily - motif 1 lies at the C-terminus 
of the putative modulating domain; motif 2 lies immediately C-terminal to
the zinc fingers; and motifs 3-8 span the hormone binding domain. Two
iterations on SPTR37_10f were required to reach convergence, at which
point a true set comprising 14 sequences was identified.
Summary Information
14 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81414141414141414
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
ECR_AEDAE     ECR_BOMMO     ECR_CHITE     ECR_DROME     
ECR_HELVI ECR_LUCCU ECR_MANSE O02035
O44336 O44337 O44338 O77240
O77255 O97095
Sequence Titles
ECR_AEDAE   ECDYSONE RECEPTOR (ECDYSTEROID RECEPTOR) (20-HYDROXY-ECDYSONE RECEPTOR) (20E REC 
ECR_BOMMO ECDYSONE RECEPTOR (ECDYSTEROID RECEPTOR) (20-HYDROXY-ECDYSONE RECEPTOR) (20E REC
ECR_CHITE ECDYSONE RECEPTOR (ECDYSTEROID RECEPTOR) (20-HYDROXY-ECDYSONE RECEPTOR) (20E REC
ECR_DROME ECDYSONE RECEPTOR (ECDYSTEROID RECEPTOR) (20-HYDROXY-ECDYSONE RECEPTOR) (20E REC
ECR_HELVI ECDYSONE RECEPTOR (ECDYSTEROID RECEPTOR) (20-HYDROXY-ECDYSONE RECEPTOR) (20E REC
ECR_LUCCU ECDYSONE RECEPTOR (ECDYSTEROID RECEPTOR) (20-HYDROXY-ECDYSONE RECEPTOR) (20E REC
ECR_MANSE ECDYSONE RECEPTOR (ECDYSTEROID RECEPTOR) (20-HYDROXY-ECDYSONE RECEPTOR) (20E REC
O02035 ECDYSONE RECEPTOR - TENEBRIO MOLITOR (YELLOW MEALWORM).
O44336 AAMECRA3 - AMBLYOMMA AMERICANUM.
O44337 AAMECRA1 - AMBLYOMMA AMERICANUM.
O44338 AAMECRA2 - AMBLYOMMA AMERICANUM.
O77240 ECDYSONE RECEPTOR-A - CHORISTONEURA FUMIFERANA (SPRUCE BUDWORM).
O77255 ECDYSTEROID RECEPTOR ECR-B - CHORISTONEURA FUMIFERANA (SPRUCE BUDWORM).
O97095 ECDYSONE RECEPTOR - LOCUSTA MIGRATORIA (MIGRATORY LOCUST).
Scan History
SPTR37_10f 2  25   NSINGLE    
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
KKGPTPRQQEEL ECR_AEDAE 178 178 -
KKGPAPRQQEEL ECR_MANSE 135 135 -
KKGPAPRVQEEL ECR_DROME 252 252 -
KKGPAPRQQEEL ECR_BOMMO 195 195 -
KKGPVPRQQEEL ECR_CHITE 103 103 -

Motif 2 width=10
Element Seqn Id St Int Rpt
RPECVVPENQ ECR_AEDAE 256 66 -
RPECVVPEST ECR_MANSE 213 66 -
RPECVVPENQ ECR_DROME 330 66 -
RPECVIQEPS ECR_BOMMO 273 66 -
RPECVVPENQ ECR_CHITE 181 66 -

Motif 3 width=26
Element Seqn Id St Int Rpt
NQMAVIYKLIWYQDGYEQPSEEDLKR ECR_AEDAE 336 70 -
NQKSLIARLVWYQEGYEQPSEEDLKR ECR_MANSE 293 70 -
NQLAVIYKLIWYQDGYEQPSEEDLRR ECR_DROME 419 79 -
NQKSLIARLVWYQEGYEQPSDEDLKR ECR_BOMMO 352 69 -
NQVAVIYKLIWYQDGYEQPSEEDLKR ECR_CHITE 278 87 -

Motif 4 width=20
Element Seqn Id St Int Rpt
EEEDQHDVHFRHITEITILT ECR_AEDAE 369 7 -
EEEEETDMPFRQITEMTILT ECR_MANSE 328 9 -
ENESQTDVSFRHITEITILT ECR_DROME 451 6 -
EEDEESDLPFRQITEMTILT ECR_BOMMO 386 8 -
EEDQEHEANFRYITEVTILT ECR_CHITE 311 7 -

Motif 5 width=14
Element Seqn Id St Int Rpt
DAATDSILFANNRS ECR_AEDAE 431 42 -
DAATDSVLFANNQA ECR_MANSE 390 42 -
DHSSDSIFFANNRS ECR_DROME 513 42 -
DAASDSVLFANNKA ECR_BOMMO 448 42 -
DHDSDSILFANNTA ECR_CHITE 373 42 -

Motif 6 width=19
Element Seqn Id St Int Rpt
IEDLLHFCRQMFSLTVDNV ECR_AEDAE 459 14 -
IEDLLHFCRCMYSMSMDNV ECR_MANSE 418 14 -
IEDLLHFCRQMFSMKVDNV ECR_DROME 541 14 -
IEDLLHFCRCMFAMGMDNV ECR_BOMMO 476 14 -
IDDLLHFCRQMYALSIDNV ECR_CHITE 401 14 -

Motif 7 width=18
Element Seqn Id St Int Rpt
LSILTELRTLGNQNSEMC ECR_AEDAE 533 55 -
LGVLTELRTLGTQNSNMC ECR_MANSE 492 55 -
LSILTELRTLGNQNAEMC ECR_DROME 615 55 -
LSVLTELRTLGTQNSNMC ECR_BOMMO 550 55 -
LGILTELRTMGNKNSEMC ECR_CHITE 475 55 -

Motif 8 width=19
Element Seqn Id St Int Rpt
SLKLKNRKLPRFLEEIWDV ECR_AEDAE 552 1 -
SLKLKNRKLPPFLEEIWDV ECR_MANSE 511 1 -
SLKLKNRKLPKFLEEIWDV ECR_DROME 634 1 -
SLKLKNRKLPPFLEEIWDV ECR_BOMMO 569 1 -
SLKLRNRKLPRFLEEVWDV ECR_CHITE 494 1 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
KKGPAPRQQEEL ECR_HELVI 151 151 -
KKGPAPRQQEEL O77240 101 101 -
KKGPAPRQQEEL O77255 129 129 -
KKGPTPRQQEEL ECR_AEDAE 178 178 -
KKGPAPRQQEEL ECR_MANSE 135 135 -
KKGPAPRLQEEL ECR_LUCCU 289 289 -
KKGPAPRVQEEL ECR_DROME 252 252 -
KKGPAPRQQEEL ECR_BOMMO 195 195 -
KKGPVPRQQEEL ECR_CHITE 103 103 -
KKGPAPRQQEEL O44336 51 51 -
KKGPAPRQQEEL O44337 167 167 -
KKGPAPRQQEEL O44338 177 177 -
KKGAAPRQQEEL O97095 168 168 -
KKGPTPRQQEEL O02035 116 116 -

Motif 2 width=10
Element Seqn Id St Int Rpt
RPECVVPENQ ECR_HELVI 229 66 -
RPECVVPETQ O77240 179 66 -
RPECVVPETQ O77255 207 66 -
RPECVVPENQ ECR_AEDAE 256 66 -
RPECVVPEST ECR_MANSE 213 66 -
RPECVVPENQ ECR_LUCCU 367 66 -
RPECVVPENQ ECR_DROME 330 66 -
RPECVIQEPS ECR_BOMMO 273 66 -
RPECVVPENQ ECR_CHITE 181 66 -
RPECVVPEYQ O44336 129 66 -
RPECVVPEYQ O44337 245 66 -
RPECVVPEYQ O44338 255 66 -
RPECVVPEYQ O97095 246 66 -
RPECVVPEVQ O02035 194 66 -

Motif 3 width=26
Element Seqn Id St Int Rpt
NQKSLIARLVWYQEGYEQPSEEDLKR ECR_HELVI 314 75 -
NQQFLIARLIWYQDGYEQPSDEDLKR O77240 259 70 -
NQQFLIARLIWYQDGYEQPSDEDLKR O77255 287 70 -
NQMAVIYKLIWYQDGYEQPSEEDLKR ECR_AEDAE 336 70 -
NQKSLIARLVWYQEGYEQPSEEDLKR ECR_MANSE 293 70 -
NQLAVIYKLIWYQDGYEQPSEEDLKR ECR_LUCCU 442 65 -
NQLAVIYKLIWYQDGYEQPSEEDLRR ECR_DROME 419 79 -
NQKSLIARLVWYQEGYEQPSDEDLKR ECR_BOMMO 352 69 -
NQVAVIYKLIWYQDGYEQPSEEDLKR ECR_CHITE 278 87 -
SQEDLINKLVYYQQEFESPSEEDMKK O44336 211 72 -
SQEDLINKLVYYQQEFESPSEEDMKK O44337 327 72 -
SQEDLINKLVYYQQEFESPSEEDMKK O44338 337 72 -
EQEELIHRLVYFQNEYESPSEEDLRR O97095 308 52 -
EELILIHRLVYFQNEYEHPSEEDVKR O02035 256 52 -

Motif 4 width=20
Element Seqn Id St Int Rpt
EDDEDSDMPFRQITEMTILT ECR_HELVI 345 5 -
DENEESDTPFRQITEMTILT O77240 294 9 -
DENEESDTPFRQITEMTILT O77255 322 9 -
EEEDQHDVHFRHITEITILT ECR_AEDAE 369 7 -
EEEEETDMPFRQITEMTILT ECR_MANSE 328 9 -
ENESQHDASFRHITEITILT ECR_LUCCU 474 6 -
ENESQTDVSFRHITEITILT ECR_DROME 451 6 -
EEDEESDLPFRQITEMTILT ECR_BOMMO 386 8 -
EEDQEHEANFRYITEVTILT ECR_CHITE 311 7 -
DSEEDNQRRFQHITEITILT O44336 244 7 -
DSEEDNQRRFQHITEITILT O44337 360 7 -
DSEEDNQRRFQHITEITILT O44338 370 7 -
EGEDQSDVRFRHITEITILT O97095 340 6 -
DGEDQCEIRFRHTTEITILT O02035 288 6 -

Motif 5 width=14
Element Seqn Id St Int Rpt
DAATDSVLFANNQA ECR_HELVI 407 42 -
DAASDSVLFANNQA O77240 355 41 -
DAASDSVLFANNQA O77255 383 41 -
DAATDSILFANNRS ECR_AEDAE 431 42 -
DAATDSVLFANNQA ECR_MANSE 390 42 -
DHNSDSIFFANNRS ECR_LUCCU 536 42 -
DHSSDSIFFANNRS ECR_DROME 513 42 -
DAASDSVLFANNKA ECR_BOMMO 448 42 -
DHDSDSILFANNTA ECR_CHITE 373 42 -
DVKTDSIVFANNQP O44336 306 42 -
DVKTDSIVFANNQP O44337 422 42 -
DVKTDSIVFANNQP O44338 432 42 -
DVNSDSILFANNQP O97095 402 42 -
DVQSDSILFVNNQP O02035 350 42 -

Motif 6 width=19
Element Seqn Id St Int Rpt
IEDLLHFCRCMYSMMMDNV ECR_HELVI 435 14 -
IEDLLHFCRCMYSMALDNI O77240 383 14 -
IEDLLHFCRCMYSMALDNI O77255 411 14 -
IEDLLHFCRQMFSLTVDNV ECR_AEDAE 459 14 -
IEDLLHFCRCMYSMSMDNV ECR_MANSE 418 14 -
IEDLLHFCRQMYSMKVDNV ECR_LUCCU 564 14 -
IEDLLHFCRQMFSMKVDNV ECR_DROME 541 14 -
IEDLLHFCRCMFAMGMDNV ECR_BOMMO 476 14 -
IDDLLHFCRQMYALSIDNV ECR_CHITE 401 14 -
ADALFRFCRKMCQLRVDNA O44336 334 14 -
ADALFRFCRKMCQLRVDNA O44337 450 14 -
ADALFRFCRKMCQLRVDNA O44338 460 14 -
IEDMLRFCRQMYAMKVDNA O97095 430 14 -
IEDLLHFCRTMYSMKVDNA O02035 378 14 -

Motif 7 width=18
Element Seqn Id St Int Rpt
LGILTEIRTLGMQNSNMC ECR_HELVI 509 55 -
LSILSELRTLGMQNSNMC O77240 457 55 -
LSILSELRTLGMQNSNMC O77255 485 55 -
LSILTELRTLGNQNSEMC ECR_AEDAE 533 55 -
LGVLTELRTLGTQNSNMC ECR_MANSE 492 55 -
LSILTELRTLGNQNAEMC ECR_LUCCU 638 55 -
LSILTELRTLGNQNAEMC ECR_DROME 615 55 -
LSVLTELRTLGTQNSNMC ECR_BOMMO 550 55 -
LGILTELRTMGNKNSEMC ECR_CHITE 475 55 -
LSILTELRTLGNMNAEMC O44336 405 52 -
LSILTELRTLGNMNAEMC O44337 521 52 -
LSILTELRTLGNMNAEMC O44338 531 52 -
LSVLTELRTLGNQNSEMC O97095 502 53 -
LSVLTELRTLGNQNSEMC O02035 450 53 -

Motif 8 width=19
Element Seqn Id St Int Rpt
SLKLKNRKLPPFLEEIWDV ECR_HELVI 528 1 -
SLKLKNRKLPPFLEEIWDV O77240 476 1 -
SLKLKNRKLPPFLEEIWDV O77255 504 1 -
SLKLKNRKLPRFLEEIWDV ECR_AEDAE 552 1 -
SLKLKNRKLPPFLEEIWDV ECR_MANSE 511 1 -
SLKLKNRKLPKFLEEIWDV ECR_LUCCU 657 1 -
SLKLKNRKLPKFLEEIWDV ECR_DROME 634 1 -
SLKLKNRKLPPFLEEIWDV ECR_BOMMO 569 1 -
SLKLRNRKLPRFLEEVWDV ECR_CHITE 494 1 -
SLKVQNKKLPPFLAEIWDI O44336 424 1 -
SLKVQNKKLPPFLAEIWDI O44337 540 1 -
SLKVQNKKLPPFLAEIWDI O44338 550 1 -
SLKLKNKKLPPFLAEIWDV O97095 521 1 -
SLKLKNKKLPPFLDEIWDV O02035 469 1 -