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PR01070

Identifier
ACCCTRFRASEB  [View Relations]  [View Alignment]  
Accession
PR01070
No. of Motifs
5
Creation Date
15-FEB-1999  (UPDATE 28-JUL-1999)
Title
Acetyl-CoA carboxylase carboxyl transferase beta subunit signature
Database References

INTERPRO; IPR000438
Literature References
1. STRYER, L.
Fatty Acid Metabolism.
IN BIOCHEMISTRY 3RD ED., W.H. FREEMAN AND CO. (NEW YORK), 1988, PP.480-485.
 
2. LI, J.J. AND CRONAN, J.E.
The genes encoding the two carboxyl transferase subunits of Escherichia coli
acetyl-CoA carboxylase.
J.BIOL.CHEM. 267(24) 16841-16847 (1992).

Documentation
Fatty acid synthesis involves a set of reactions, commencing with 
carboxylation of acetyl-CoA to malonyl-CoA [1]. This is an irreversible
reaction, catalysed by the acetyl-CoA carboxylase complex (EC 6.4.1.2); a
heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and two
non-identical carboxyl transferase subunits (alpha and beta) in a 2:2
association [1,2]. 
 
The reaction involves two steps: 
 
(1) Biotin carrier protein + ATP + HCO(3)(-) <=>
	    Carboxybiotin carrier protein + ADP + Phosphate
 
(2) Carboxybiotin carrier protein + Acetyl-CoA ->
	    Malonyl-CoA + Biotin carrier protein
 
In the first step, biotin carboxylase catalyses the carboxylation of the
carrier protein to form an intermediate. Next, the transcarboxylase complex
transfers the carboxyl group from the intermediate to acetyl-CoA forming
malonyl-CoA.
 
ACCCTRFRASEB is a 5-element fingerprint that provides a signature for the
acetyl-CoA carboxylase carboxyl transferase beta subunit. The fingerprint
was derived from an initial alignment of 5 sequences: the motifs were drawn
from conserved regions within the central and C-terminal portions of the
alignment. Three iterations on OWL31.1 were required to reach convergence,
at which point a true set comprising 23 sequences was identified. Several
partial matches were also found, all of which are transferase beta subunit
fragments that lack portions of sequence bearing one or more motifs.
 
An update on SPTR37_9f identified a true set of 23 sequences, and 3
partial matches.
Summary Information
  23 codes involving  5 elements
0 codes involving 4 elements
1 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
52323232323
400000
300111
222000
12345
True Positives
ACCD_ANGLY    ACCD_BRANA    ACCD_CHLVU    ACCD_CUSRE    
ACCD_ECOLI ACCD_EPIVI ACCD_HAEIN ACCD_MARPO
ACCD_MYCTU ACCD_PEA ACCD_PHYPA ACCD_PICAB
ACCD_PINTH ACCD_PORPU ACCD_SOYBN ACCD_SYNP7
ACCD_SYNY3 ACCD_TOBAC O25604 O34571
O66754 O78323 O84295
True Positive Partials
Codes involving 3 elements
Q50132
Codes involving 2 elements
ACCD_ORYSA PCCB_SACER
Sequence Titles
ACCD_ANGLY  ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - ANGIOPTERIS LYGODIIFOLIA (TURNIP FERN). 
ACCD_BRANA ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - BRASSICA NAPUS (RAPE).
ACCD_CHLVU ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - CHLORELLA VULGARIS.
ACCD_CUSRE ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - CUSCUTA REFLEXA (SOUTHERN ASIAN DODDER).
ACCD_ECOLI ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - ESCHERICHIA COLI.
ACCD_EPIVI ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - EPIFAGUS VIRGINIANA (BEECHDROPS).
ACCD_HAEIN ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - HAEMOPHILUS INFLUENZAE.
ACCD_MARPO ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - MARCHANTIA POLYMORPHA (LIVERWORT).
ACCD_MYCTU PUTATIVE ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - MYCOBACTERIUM TUBERCULOSIS.
ACCD_PEA ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - PISUM SATIVUM (GARDEN PEA).
ACCD_PHYPA ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - PHYSCOMITRELLA PATENS (MOSS).
ACCD_PICAB ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - PICEA ABIES (NORWAY SPRUCE) (PICEA EXCELSA).
ACCD_PINTH ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - PINUS THUNBERGII (GREEN PINE) (JAPANESE BLACK PINE).
ACCD_PORPU ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - PORPHYRA PURPUREA.
ACCD_SOYBN ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - GLYCINE MAX (SOYBEAN).
ACCD_SYNP7 ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - SYNECHOCOCCUS SP. (STRAIN PCC 7942) (ANACYSTIS NIDULANS R2).
ACCD_SYNY3 ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
ACCD_TOBAC ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - NICOTIANA TABACUM (COMMON TOBACCO).
O25604 ACETYL-COA CARBOXYLASE BETA SUBUNIT (ACCD) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
O34571 ACETYL-COA CARBOXYLASE SUBUNIT - BACILLUS SUBTILIS.
O66754 ACETYL-COA CARBOXYLTRANSFERASE BETA SUBUNIT - AQUIFEX AEOLICUS.
O78323 ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE - SOLANUM TUBEROSUM (POTATO).
O84295 ACCOA CARBOXYLASE/TRANSFERASE BETA - CHLAMYDIA TRACHOMATIS.

Q50132 HYPOTHETICAL 21.3 KD PROTEIN ZNFA - MYCOBACTERIUM LEPRAE.

ACCD_ORYSA ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) - ORYZA SATIVA (RICE).
PCCB_SACER PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) (PCCASE) (PROPANOYL-COA:CARBON DIOXIDE LIGASE) - SACCHAROPOLYSPORA ERYTHRAEA (STREPTOMYCES ERYTHRAEUS).
Scan History
OWL31_1    3  40   NSINGLE    
SPTR37_9f 2 50 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
FHFMGGSMGSVVGEK ACCD_CHLVU 136 136 -
FQSMGGSMGSVVGEK ACCD_ANGLY 151 151 -
FQFMGGSMGSVVGDK ACCD_BRANA 327 327 -
FAFMGGSMGSVVGAK ACCD_HAEIN 129 129 -
FAFMGGSMGSVVGAR ACCD_ECOLI 127 127 -

Motif 2 width=19
Element Seqn Id St Int Rpt
ASGGARMQEGILSLMQMAK ACCD_CHLVU 170 19 -
ASGGARMQEGTLSSMQMAK ACCD_ANGLY 185 19 -
SSGGARMQEGSLSLMQMAK ACCD_BRANA 361 19 -
ASGGARMQEALFSLMQMAK ACCD_HAEIN 163 19 -
ASGGARMQEALMSLMQMAK ACCD_ECOLI 161 19 -

Motif 3 width=18
Element Seqn Id St Int Rpt
LTSPTTGGVTASFAMLGD ACCD_CHLVU 208 19 -
LTYPTTGGVTASFGMLGD ACCD_ANGLY 223 19 -
LTSPTTGGVTASLGMLGD ACCD_BRANA 399 19 -
LTDPTLGGVSASFAMLGD ACCD_HAEIN 200 18 -
LTDPTMGGVSASFAMLGD ACCD_ECOLI 198 18 -

Motif 4 width=12
Element Seqn Id St Int Rpt
FAGRRVIEQTLQ ACCD_CHLVU 237 11 -
FAGKRVIEQTLR ACCD_ANGLY 252 11 -
FAGKRVIEQTLK ACCD_BRANA 428 11 -
FAGPRVIEQTVR ACCD_HAEIN 229 11 -
FAGPRVIEQTVR ACCD_ECOLI 227 11 -

Motif 5 width=10
Element Seqn Id St Int Rpt
EYLLHHGLLD ACCD_CHLVU 259 10 -
ESLFDHGLLD ACCD_ANGLY 274 10 -
ESLLRKGLLD ACCD_BRANA 450 10 -
EFLLEKGAID ACCD_HAEIN 251 10 -
EFLIEKGAID ACCD_ECOLI 249 10 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
FQFMGGSMGSVVGEK ACCD_TOBAC 348 348 -
FQFMGGSMGSVVGEK O78323 343 343 -
FQFMGGSMGSVVGEK ACCD_EPIVI 336 336 -
FQFMGGSMGSVVGEK ACCD_PINTH 164 164 -
FQFMGGSMGSVVGEK ACCD_MARPO 151 151 -
FKFMGGSMGSVVGEK ACCD_CUSRE 323 323 -
FHFMGGSMGSVVGEK ACCD_CHLVU 136 136 -
FQFMGGSMGSAVGEK ACCD_SOYBN 270 270 -
FQSMGGSMGSVVGEK ACCD_ANGLY 151 151 -
FQFMGGSMGSVVGEK ACCD_PHYPA 151 151 -
FRFMGGSMGSVVGEK ACCD_PORPU 134 134 -
FRFMGGSMGSVVGEK ACCD_SYNY3 136 136 -
FQFMGGSMGSVVGDK ACCD_BRANA 327 327 -
FRFMGGSMGSVVGEK ACCD_SYNP7 133 133 -
FKFMGGSMGSVVGEK ACCD_PICAB 164 164 -
FAFMGGSMGSVVGAK ACCD_HAEIN 129 129 -
FNFIGGSMGSVVGER O66754 121 121 -
FAFMGGSMGSVVGAR ACCD_ECOLI 127 127 -
SEFIAGSMGCVVGEK ACCD_PEA 433 433 -
FSFMGGSLGSVEGEK O25604 131 131 -
SSFRMGSMGSVVGEK O34571 101 101 -
FSFMAGSMGAVVGEK O84295 127 127 -
FDFLGGSIGVAAAER ACCD_MYCTU 71 71 -

Motif 2 width=19
Element Seqn Id St Int Rpt
ASGGARMQEGSLSLMQMAK ACCD_TOBAC 382 19 -
ASGGARMQEGSLSLMQMAK O78323 377 19 -
ASGGARMQEGSLSLMQMAK ACCD_EPIVI 370 19 -
ASGGARMQEGSFSLMQMAK ACCD_PINTH 198 19 -
SSGGARMQEGTLSLMQMAK ACCD_MARPO 185 19 -
ASGGARMQEGSLSLMQMAK ACCD_CUSRE 357 19 -
ASGGARMQEGILSLMQMAK ACCD_CHLVU 170 19 -
ASGGARMQEGSLSLIQMAK ACCD_SOYBN 304 19 -
ASGGARMQEGTLSSMQMAK ACCD_ANGLY 185 19 -
SSGGARMQEGTLSLMQMAK ACCD_PHYPA 185 19 -
ASGGARMQEGMLSLMQMAK ACCD_PORPU 168 19 -
ASGGARMQEGMLSLMQMAK ACCD_SYNY3 170 19 -
SSGGARMQEGSLSLMQMAK ACCD_BRANA 361 19 -
ASGGARMQEGMLSLMQMAK ACCD_SYNP7 167 19 -
ASGGARMQEGSFSSMQMAK ACCD_PICAB 198 19 -
ASGGARMQEALFSLMQMAK ACCD_HAEIN 163 19 -
ASGGARMQEGILSLMQMAK O66754 155 19 -
ASGGARMQEALMSLMQMAK ACCD_ECOLI 161 19 -
ASGGARMQEGSLSLMQMAK ACCD_PEA 467 19 -
ASGGARMQESTYSLMQMAK O25604 165 19 -
ASGGARMQEGVLSLMQMAK O34571 135 19 -
ASGGARMQESVFSLMQMAK O84295 161 19 -
SSGGTRMQEGTVAFLQMVK ACCD_MYCTU 105 19 -

Motif 3 width=18
Element Seqn Id St Int Rpt
LTSPTTGGVTASFGMLGD ACCD_TOBAC 420 19 -
LTSPTTGGVTASFGMLGD O78323 415 19 -
LTSPTTGGVTASFGMLGD ACCD_EPIVI 408 19 -
LTSPTTGGVTASFGMLGD ACCD_PINTH 236 19 -
LTYPTTGGVTASFGMLGD ACCD_MARPO 223 19 -
LTSPTAGGVTASFGMLGD ACCD_CUSRE 395 19 -
LTSPTTGGVTASFAMLGD ACCD_CHLVU 208 19 -
LTSPTTGGVTASFGMLGD ACCD_SOYBN 342 19 -
LTYPTTGGVTASFGMLGD ACCD_ANGLY 223 19 -
LTYPTTGGVTASFGMLGD ACCD_PHYPA 223 19 -
LTSPTTGGVTASFAMLGD ACCD_PORPU 205 18 -
LTHPTTGGVTASFAMLGD ACCD_SYNY3 207 18 -
LTSPTTGGVTASLGMLGD ACCD_BRANA 399 19 -
LTHPTTGGVTASFAMLGD ACCD_SYNP7 204 18 -
LTSPTTGGVTASFGMLGD ACCD_PICAB 236 19 -
LTDPTLGGVSASFAMLGD ACCD_HAEIN 200 18 -
LTNPTMGGVSASFAFLGD O66754 192 18 -
LTDPTMGGVSASFAMLGD ACCD_ECOLI 198 18 -
LTSPTTGGVTASFGMLGD ACCD_PEA 505 19 -
LSDPTYGGVSASFAFLGD O25604 202 18 -
MTHPTTGGVSASFASLGD O34571 172 18 -
LTNPTSGGVTASFASLGD O84295 198 18 -
LRHPTTGGVFASWGSLGH ACCD_MYCTU 142 18 -

Motif 4 width=12
Element Seqn Id St Int Rpt
FAGKRVIEQTLN ACCD_TOBAC 449 11 -
FAGKRVIEQTLN O78323 444 11 -
FAGKRVIEQTLH ACCD_EPIVI 437 11 -
FAGKRVIEQTLG ACCD_PINTH 265 11 -
FAGKRVIEQTLR ACCD_MARPO 252 11 -
FAGKRVIEQTLN ACCD_CUSRE 424 11 -
FAGRRVIEQTLQ ACCD_CHLVU 237 11 -
FAGKRVIEQTLN ACCD_SOYBN 371 11 -
FAGKRVIEQTLR ACCD_ANGLY 252 11 -
FAGKRVIEQTLR ACCD_PHYPA 252 11 -
FAGRRVIEQTIK ACCD_PORPU 234 11 -
FAGRRVIEQTLR ACCD_SYNY3 236 11 -
FAGKRVIEQTLK ACCD_BRANA 428 11 -
FAGRRVIEQTLR ACCD_SYNP7 233 11 -
FAGKIVIDQTLG ACCD_PICAB 265 11 -
FAGPRVIEQTVR ACCD_HAEIN 229 11 -
FAGPRVIEQTIK O66754 221 11 -
FAGPRVIEQTVR ACCD_ECOLI 227 11 -
FAGKRVIEQLLN ACCD_PEA 534 11 -
FAGPRVIKQTIG O25604 231 11 -
FAGRRIIEQTIG O34571 201 11 -
FAGPRVVSQVIG O84295 227 11 -
FLGPRVYELLYG ACCD_MYCTU 171 11 -

Motif 5 width=10
Element Seqn Id St Int Rpt
EYLFQKGLFD ACCD_TOBAC 471 10 -
EYLFQKGLFD O78323 466 10 -
EYLFQKGLFD ACCD_EPIVI 459 10 -
EHLFGHGLFD ACCD_PINTH 287 10 -
ESLFDHGLLD ACCD_MARPO 274 10 -
EFLFHKGLFD ACCD_CUSRE 446 10 -
EYLLHHGLLD ACCD_CHLVU 259 10 -
EYLFHKGLFD ACCD_SOYBN 393 10 -
ESLFDHGLLD ACCD_ANGLY 274 10 -
ESLFYSGLLD ACCD_PHYPA 274 10 -
EYLFEHGFLD ACCD_PORPU 256 10 -
EYLLHHGFVD ACCD_SYNY3 258 10 -
ESLLRKGLLD ACCD_BRANA 450 10 -
EYLQAHGFVD ACCD_SYNP7 255 10 -
EHLFGHGLFD ACCD_PICAB 287 10 -
EFLLEKGAID ACCD_HAEIN 251 10 -
EFLLEKGQID O66754 243 10 -
EFLIEKGAID ACCD_ECOLI 249 10 -
DLLFDRGLLD ACCD_PEA 556 10 -
EFLLEHGLID O25604 253 10 -
EFLLKHGQLD O34571 223 10 -
EFLLEHGMID O84295 249 10 -
ENLRRHGIID ACCD_MYCTU 193 10 -