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PR01010

Identifier
FLGPRINGFLGI  [View Relations]  [View Alignment]  
Accession
PR01010
No. of Motifs
9
Creation Date
26-NOV-1998  (UPDATE 15-JUN-1999)
Title
Flagellar P-ring protein signature
Database References

INTERPRO; IPR001782
Literature References
1. JONES, C.J., HOMMA, M. AND MACNAB, R.M.
L-, P-, and M-ring proteins of the flagellar basal body of Salmonella
typhimurium: gene sequences and deduced protein sequences.
J.BACTERIOL. 171 3890-3900 (1989).
 
2. HOMMA, M., KUTSUKAKE, K., HASEBE, M., IINO, T. AND MACNAB, R.M.
FlgB, FlgC, FlgF and FlgG. A family of structurally related proteins in 
the flagellar basal body of Salmonella typhimurium.
J.MOL.BIOL. 211 465-477 (1990). 
 
3.HOMMA, M., KOMEDA, Y., IINO, T. AND MACNAB, R.M. 
The flaFIX gene product of Salmonella typhimurium is a flagellar basal body
component with a signal peptide for export.
J.BACTERIOL. 169 1493-1498 (1987). 

Documentation
The flgH, flgI and fliF genes of Salmonella typhimurium encode the major 
proteins for the L, P and M rings of the flagellar basal body [1]. In fact,
the basal body consists of four rings (L,P,S and M) surrounding the
flagellar rod, which is believed to transmit motor rotation to the filament 
[2]. The M ring is integral to the inner membrane of the cell, and may be
connected to the rod via the S (supramembrane) ring, which lies just distal
to it. The L and P rings reside in the outer membrane and periplasmic space,
respectively.
 
The sequences of the FlgH, FlgI and FliF gene products have been determined
[1]. FlgH and FlgI, which are exported across the cell membrane to their 
destinations in the outer membrane and periplasmic space, have typical N-
terminal cleaved signal-peptide sequences [1,3]. FlgH is predicted to have
an extensive beta-sheet structure, in keeping with other outer membrane 
proteins, and FlgI is thought to have even more beta-structure content [1].
Several aspects of the DNA sequence of these genes and their surrounds
suggest complex regulation of the flagellar gene system. 
 
FLGPRINGFLGI is a 9-element fingerprint that provides a signature for
flagellar P-ring proteins. The fingerprint was derived from an initial 
alignment of 6 sequences: the motifs were drawn from conserved regions 
spanning the N-terminal two-thirds of the alignment. Three iterations on 
OWL30.2 were required to reach convergence, at which point a true set 
comprising 9 sequences was identified.
 
An update on SPTR37_9f identified a true set of 10 sequences, and 4
partial matches.
Summary Information
10 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9101010101010101010
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
FLGI_AGRTU    FLGI_CAUCR    FLGI_ECOLI    FLGI_PSEPU    
FLGI_RHIME FLGI_SALTY O25028 O30854
O51713 O67608
Sequence Titles
FLGI_AGRTU  FLAGELLAR P-RING PROTEIN PRECURSOR - AGROBACTERIUM TUMEFACIENS. 
FLGI_CAUCR FLAGELLAR P-RING PROTEIN PRECURSOR - CAULOBACTER CRESCENTUS.
FLGI_ECOLI FLAGELLAR P-RING PROTEIN PRECURSOR - ESCHERICHIA COLI.
FLGI_PSEPU FLAGELLAR P-RING PROTEIN PRECURSOR - PSEUDOMONAS PUTIDA.
FLGI_RHIME FLAGELLAR P-RING PROTEIN PRECURSOR - RHIZOBIUM MELILOTI.
FLGI_SALTY FLAGELLAR P-RING PROTEIN PRECURSOR - SALMONELLA TYPHIMURIUM.
O25028 FLAGELLAR BASAL-BODY P-RING PROTEIN (FLGI) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
O30854 FLGI - VIBRIO CHOLERAE.
O51713 FLAGELLAR P-RING PROTEIN (FLGI) - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
O67608 FLAGELLAR P-RING PROTEIN FLGI - AQUIFEX AEOLICUS.
Scan History
OWL30_2    3  100  NSINGLE    
SPTR37_9f 3 134 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
RIKDIASLQAGRDNQL FLGI_AGRTU 30 30 -
RIRDLTSVQGVRENSL FLGI_SALTY 21 21 -
RIRDLTSVQGVRQNSL FLGI_ECOLI 21 21 -
RIKDVASLQSGRDNQL FLGI_RHIME 28 28 -
RLKDIASISGVRSNQL FLGI_PSEPU 24 24 -
RIKDIVSFEGVRENQL FLGI_CAUCR 29 29 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LIGYGLVVGLQGTGDS FLGI_AGRTU 45 -1 -
LIGYGLVVGLDGTGDQ FLGI_SALTY 36 -1 -
LIGYGLVVGLDGTGDQ FLGI_ECOLI 36 -1 -
LIGYGLVVGLQGTGDS FLGI_RHIME 43 -1 -
LIGYGLVVGLNGTGDQ FLGI_PSEPU 39 -1 -
LIGYGIVVGLNGTGDS FLGI_CAUCR 44 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
PFTEQSMRAMLQNLGI FLGI_AGRTU 65 4 -
PFTTQTLNNMLSQLGI FLGI_SALTY 56 4 -
PFTTQTLNNMLSQLGI FLGI_ECOLI 56 4 -
PFTDQSIRAMLQNLGI FLGI_RHIME 63 4 -
PFTLQTFNNMLSQFGI FLGI_PSEPU 59 4 -
PMTKQSLEAMLERQGV FLGI_CAUCR 64 4 -

Motif 4 width=20
Element Seqn Id St Int Rpt
KNIAAVMVTANLPPFASPGS FLGI_AGRTU 90 9 -
KNVAAVMVTASYPPFARQGQ FLGI_SALTY 82 10 -
KNVAAVMVTASLPPFGRQGQ FLGI_ECOLI 82 10 -
RNVAAVLVTATLPPFASPGS FLGI_RHIME 88 9 -
KNVAAVSVHADLPPFAKPGQ FLGI_PSEPU 86 11 -
KNTAAVMVTANLPPFSASGS FLGI_CAUCR 89 9 -

Motif 5 width=18
Element Seqn Id St Int Rpt
DVTVSSLGDATSLRGGNL FLGI_AGRTU 112 2 -
DVVVSSMGNAKSLRGGTL FLGI_SALTY 104 2 -
DVVVSSMGNAKSLRGGTL FLGI_ECOLI 104 2 -
DVTVGSLGDATSLRGGTL FLGI_RHIME 110 2 -
DITVSSIGNSKSLRGGSL FLGI_PSEPU 108 2 -
DVTVSTLGDAKSLLGGTL FLGI_CAUCR 111 2 -

Motif 6 width=19
Element Seqn Id St Int Rpt
LIMTSLSGADGQIYAVAQG FLGI_AGRTU 129 -1 -
LLMTPLKGVDSQVYALAQG FLGI_SALTY 121 -1 -
LLMTPLKGVDSQVYALAQG FLGI_ECOLI 121 -1 -
LVMTSLSGADGQIYAVAQG FLGI_RHIME 127 -1 -
LLMTPLKGIDGNVYAIAQG FLGI_PSEPU 125 -1 -
LLVTSLQGADGQTYAVAQG FLGI_CAUCR 128 -1 -

Motif 7 width=19
Element Seqn Id St Int Rpt
TSARVPNGAIIERELPSKF FLGI_AGRTU 169 21 -
NGGRITNGAIIERELPTQF FLGI_SALTY 161 21 -
NGGRITNGAVIERELPSQF FLGI_ECOLI 161 21 -
TAGRVPNGAIIERELPSKF FLGI_RHIME 167 21 -
SAGRIPGGASVERAVPSGF FLGI_PSEPU 166 22 -
TAGRIAGGGVIERETGFQM FLGI_CAUCR 169 22 -

Motif 8 width=19
Element Seqn Id St Int Rpt
LQLRNPDFSTAVRVADVVN FLGI_AGRTU 195 7 -
LQLNDEDFTMAQQITDAIN FLGI_SALTY 187 7 -
LQLNDEDFSMAQQIADTIN FLGI_ECOLI 187 7 -
LQLRNPDFSTAVGMAAAIN FLGI_RHIME 193 7 -
LNLNRPDFTTAKRIVDKVN FLGI_PSEPU 192 7 -
LTLRNPDFTTARRVADAIN FLGI_CAUCR 195 7 -

Motif 9 width=13
Element Seqn Id St Int Rpt
AKVVINERTGTIV FLGI_AGRTU 261 47 -
AKVVINSRTGSVV FLGI_SALTY 251 45 -
AKVVINSRTGSVV FLGI_ECOLI 251 45 -
ARVVINERTGTIV FLGI_RHIME 259 47 -
AKVIINSRTGTIV FLGI_PSEPU 256 45 -
AKVVIDEVAGVIV FLGI_CAUCR 256 42 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
RIKDIASLQAGRDNQL FLGI_AGRTU 30 30 -
RIRDLTSVQGVRENSL FLGI_SALTY 21 21 -
RIRDLTSVQGVRQNSL FLGI_ECOLI 21 21 -
RIKDVASLQSGRDNQL FLGI_RHIME 28 28 -
RLKDIASISGVRSNQL FLGI_PSEPU 24 24 -
RIKDVAQVAGVRNNQL O30854 20 20 -
RIKDIVSFEGVRENQL FLGI_CAUCR 29 29 -
RIKDIATIEGNRYNYL O67608 23 23 -
KIGDIASVVGVRDNQL O25028 21 21 -
KLKEIADIYPTNTNFL O51713 41 41 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LIGYGLVVGLQGTGDS FLGI_AGRTU 45 -1 -
LIGYGLVVGLDGTGDQ FLGI_SALTY 36 -1 -
LIGYGLVVGLDGTGDQ FLGI_ECOLI 36 -1 -
LIGYGLVVGLQGTGDS FLGI_RHIME 43 -1 -
LIGYGLVVGLNGTGDQ FLGI_PSEPU 39 -1 -
LVGYGLVTGLPGTGES O30854 35 -1 -
LIGYGIVVGLNGTGDS FLGI_CAUCR 44 -1 -
LIGYGLVVGLKGTGDG O67608 38 -1 -
LIGYGLVIGLNGTGDK O25028 36 -1 -
LTGIGIVAGLAGKGDS O51713 56 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
PFTEQSMRAMLQNLGI FLGI_AGRTU 65 4 -
PFTTQTLNNMLSQLGI FLGI_SALTY 56 4 -
PFTTQTLNNMLSQLGI FLGI_ECOLI 56 4 -
PFTDQSIRAMLQNLGI FLGI_RHIME 63 4 -
PFTLQTFNNMLSQFGI FLGI_PSEPU 59 4 -
PFTDQSFNAMLQSFGI O30854 52 1 -
PMTKQSLEAMLERQGV FLGI_CAUCR 64 4 -
QFTVQSLANMLRRMGI O67608 57 3 -
KFTMQSISNMLESVNV O25028 55 3 -
ILEENNIINEIGSNNI O51713 81 9 -

Motif 4 width=20
Element Seqn Id St Int Rpt
KNIAAVMVTANLPPFASPGS FLGI_AGRTU 90 9 -
KNVAAVMVTASYPPFARQGQ FLGI_SALTY 82 10 -
KNVAAVMVTASLPPFGRQGQ FLGI_ECOLI 82 10 -
RNVAAVLVTATLPPFASPGS FLGI_RHIME 88 9 -
KNVAAVSVHADLPPFAKPGQ FLGI_PSEPU 86 11 -
KNVAAVIVTADLPAFSKQGQ O30854 78 10 -
KNTAAVMVTANLPPFSASGS FLGI_CAUCR 89 9 -
KNVAAVMVTAKVPPYAKAGM O67608 82 9 -
KNVAAVMITASLPPFARQGD O25028 80 9 -
KNIALVNVSLQVKGNTIKGS O51713 99 2 -

Motif 5 width=18
Element Seqn Id St Int Rpt
DVTVSSLGDATSLRGGNL FLGI_AGRTU 112 2 -
DVVVSSMGNAKSLRGGTL FLGI_SALTY 104 2 -
DVVVSSMGNAKSLRGGTL FLGI_ECOLI 104 2 -
DVTVGSLGDATSLRGGTL FLGI_RHIME 110 2 -
DITVSSIGNSKSLRGGSL FLGI_PSEPU 108 2 -
DITVSSIGSAKSLRGGTL O30854 100 2 -
DVTVSTLGDAKSLLGGTL FLGI_CAUCR 111 2 -
DVEVSSIGDAKSLEGGTL O67608 104 2 -
DIHISSIGDAKSIQGGTL O25028 102 2 -
KACVASILDSKDLTNGIL O51713 121 2 -

Motif 6 width=19
Element Seqn Id St Int Rpt
LIMTSLSGADGQIYAVAQG FLGI_AGRTU 129 -1 -
LLMTPLKGVDSQVYALAQG FLGI_SALTY 121 -1 -
LLMTPLKGVDSQVYALAQG FLGI_ECOLI 121 -1 -
LVMTSLSGADGQIYAVAQG FLGI_RHIME 127 -1 -
LLMTPLKGIDGNVYAIAQG FLGI_PSEPU 125 -1 -
LMQTFLKGLDGQVYAVAQG O30854 117 -1 -
LLVTSLQGADGQTYAVAQG FLGI_CAUCR 128 -1 -
LLMTPLRGPDGKVYAIAQG O67608 121 -1 -
LVMTPLNAVDGNIYALAQG O25028 119 -1 -
LLKTNLKNKEGEIIAIASG O51713 138 -1 -

Motif 7 width=19
Element Seqn Id St Int Rpt
TSARVPNGAIIERELPSKF FLGI_AGRTU 169 21 -
NGGRITNGAIIERELPTQF FLGI_SALTY 161 21 -
NGGRITNGAVIERELPSQF FLGI_ECOLI 161 21 -
TAGRVPNGAIIERELPSKF FLGI_RHIME 167 21 -
SAGRIPGGASVERAVPSGF FLGI_PSEPU 166 22 -
TVGMISSGAIVEREVPNPF O30854 158 22 -
TAGRIAGGGVIERETGFQM FLGI_CAUCR 169 22 -
TVGRIPNGAILEKDLPFSA O67608 161 21 -
LSANIINGATIEREVSYDL O25028 148 10 -
NAKIGTFTFSIEKDNQNIF O51713 258 101 -

Motif 8 width=19
Element Seqn Id St Int Rpt
LQLRNPDFSTAVRVADVVN FLGI_AGRTU 195 7 -
LQLNDEDFTMAQQITDAIN FLGI_SALTY 187 7 -
LQLNDEDFSMAQQIADTIN FLGI_ECOLI 187 7 -
LQLRNPDFSTAVGMAAAIN FLGI_RHIME 193 7 -
LNLNRPDFTTAKRIVDKVN FLGI_PSEPU 192 7 -
FNLFESDFTTAQRLADAVN O30854 184 7 -
LTLRNPDFTTARRVADAIN FLGI_CAUCR 195 7 -
IYLDEPDFTTAKNVQDVIN O67608 186 6 -
LSLKNPNFKNAIQVQNTLN O25028 174 7 -
IFLSKNNKTTIQVNSMKLN O51713 275 -2 -

Motif 9 width=13
Element Seqn Id St Int Rpt
AKVVINERTGTIV FLGI_AGRTU 261 47 -
AKVVINSRTGSVV FLGI_SALTY 251 45 -
AKVVINSRTGSVV FLGI_ECOLI 251 45 -
ARVVINERTGTIV FLGI_RHIME 259 47 -
AKVIINSRTGTIV FLGI_PSEPU 256 45 -
AKIIVNSRTGTIV O30854 248 45 -
AKVVIDEVAGVIV FLGI_CAUCR 256 42 -
AKVVIDGRSGIVL O67608 248 43 -
NKIIVDEKSGTIV O25028 236 43 -
AAQKINKLNGELI O51713 314 20 -