1. JONES, C.J., HOMMA, M. AND MACNAB, R.M.
L-, P-, and M-ring proteins of the flagellar basal body of Salmonella
typhimurium: gene sequences and deduced protein sequences.
J.BACTERIOL. 171 3890-3900 (1989).
2. HOMMA, M., KUTSUKAKE, K., HASEBE, M., IINO, T. AND MACNAB, R.M.
FlgB, FlgC, FlgF and FlgG. A family of structurally related proteins in
the flagellar basal body of Salmonella typhimurium.
J.MOL.BIOL. 211 465-477 (1990).
3.HOMMA, M., KOMEDA, Y., IINO, T. AND MACNAB, R.M.
The flaFIX gene product of Salmonella typhimurium is a flagellar basal body
component with a signal peptide for export.
J.BACTERIOL. 169 1493-1498 (1987).
The flgH, flgI and fliF genes of Salmonella typhimurium encode the major
proteins for the L, P and M rings of the flagellar basal body . In fact,
the basal body consists of four rings (L,P,S and M) surrounding the
flagellar rod, which is believed to transmit motor rotation to the filament
. The M ring is integral to the inner membrane of the cell, and may be
connected to the rod via the S (supramembrane) ring, which lies just distal
to it. The L and P rings reside in the outer membrane and periplasmic space,
The sequences of the FlgH, FlgI and FliF gene products have been determined
. FlgH and FlgI, which are exported across the cell membrane to their
destinations in the outer membrane and periplasmic space, have typical N-
terminal cleaved signal-peptide sequences [1,3]. FlgH is predicted to have
an extensive beta-sheet structure, in keeping with other outer membrane
proteins, and FlgI is thought to have even more beta-structure content .
Several aspects of the DNA sequence of these genes and their surrounds
suggest complex regulation of the flagellar gene system.
FLGPRINGFLGI is a 9-element fingerprint that provides a signature for
flagellar P-ring proteins. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved regions
spanning the N-terminal two-thirds of the alignment. Three iterations on
OWL30.2 were required to reach convergence, at which point a true set
comprising 9 sequences was identified.
An update on SPTR37_9f identified a true set of 10 sequences, and 4