SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00990

Identifier
RIBOKINASE  [View Relations]  [View Alignment]  
Accession
PR00990
No. of Motifs
5
Creation Date
25-OCT-1998  (UPDATE 23-JUN-1999)
Title
Ribokinase signature
Database References

PROSITE; PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2
INTERPRO; IPR002139
Literature References
1. SIGRELL, J.A., CAMERON, A.D., JONES, T.A. AND MOWBRAY, S.L.
Purification, characterization, and crystallization of Eschericha coli
ribokinase.
PROTEIN SCI. 6 2474-2476 (1997).
 
2. SPYCHALA, J., DATTA, N.S., TAKABAYASHI, K., DATTA, M., FOX, I.H.,
GRIBBIN, T. AND MITCHELL, B.S.
Cloning of human adenosine kinase cDNA: Sequence similarity to microbrial
ribokinases and fructokinases.
PROC.NATL.ACAD.SCI.U.S.A. 93 1232-1237 (1996).
 
3. SINGH, B., HAO, W.H., WU, Z.C., EIGL, B. AND GUPTA, R.S.
Cloning and characterization of cDNA for adenosine kinase from mammalian
Chinese hamster ovary cells involve structural alterations in the gene.
EUR.J.BIOCHEMISTRY 241 564-571 (1996).

Documentation
Ribokinases participate in the first step of ribose metabolism, and are 
members of the superfamily of carbohydrate kinases. Ribokinases phosphory-
late ribose to ribose-5-phosphate in the presence of ATP and magnesium [1]:
 
	   ATP + D-Ribose = ADP + D-Ribose-5-Phosphate
 
The phosphorylated sugar may then enter the pentose phosphate pathway [2].
 
There are indications that the phosphorylated sugar may also be used in the
synthesis of amino acids (histidine and tryptophan). Further, links to
mammalian adenosine kinase have been identified, through sequence
similarity, suggesting possible homology [2,3].
 
RIBOKINASE is a 5-element fingerprint that provides a signature for
ribokinases. The fingerprint was derived from an initial alignment of 5 
sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length. Two iterations on OWL30.2 were required to reach
convergence, at which point a true set comprising 8 sequences was identified.
Two partial matches were also found, both of which are related fructokinases
that match motifs 2 and 5.
 
An update on SPTR37_9f identified a true set of 9 sequences, and 5
partial matches.
Summary Information
   9 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
5 codes involving 2 elements
Composite Feature Index
599999
400000
300000
205005
12345
True Positives
O77425        O86458        P71913        Q19133        
RBSK_BACSU RBSK_ECOLI RBSK_HAEIN RBSK_SCHPO
RBSK_YEAST
True Positive Partials
Codes involving 2 elements
O04897 Q42645 SCRK_SALTY SCRK_VIBAL
YURL_BACSU
Sequence Titles
O77425      EG:115C2.1 PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY). 
O86458 RBSK - RHIZOBIUM ETLI.
P71913 HYPOTHETICAL 30.3 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
Q19133 F07A11.5 PROTEIN - CAENORHABDITIS ELEGANS.
RBSK_BACSU RIBOKINASE (EC 2.7.1.15) - BACILLUS SUBTILIS.
RBSK_ECOLI RIBOKINASE (EC 2.7.1.15) - ESCHERICHIA COLI.
RBSK_HAEIN RIBOKINASE (EC 2.7.1.15) - HAEMOPHILUS INFLUENZAE.
RBSK_SCHPO PUTATIVE RIBOKINASE (EC 2.7.1.15) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
RBSK_YEAST PROBABLE RIBOKINASE (EC 2.7.1.15) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).

O04897 FRUCTOKINASE (EC 2.7.1.4) (D-FRUCTOKINASE) - LYCOPERSICON ESCULENTUM (TOMATO).
Q42645 FRUCTOKINASE (EC 2.7.1.4) (D-FRUCTOKINASE) - BETA VULGARIS (SUGAR BEET).
SCRK_SALTY FRUCTOKINASE (EC 2.7.1.4) - SALMONELLA TYPHIMURIUM.
SCRK_VIBAL FRUCTOKINASE (EC 2.7.1.4) - VIBRIO ALGINOLYTICUS.
YURL_BACSU HYPOTHETICAL SUGAR KINASE IN HOM-MRGA INTERGENIC REGION - BACILLUS SUBTILIS.
Scan History
OWL30_2    2  50   NSINGLE    
SPTR37_9f 1 100 NSINGLE
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
VLGSINADHVISVPYFTKPGET RBSK_HAEIN 7 7 -
VLGSINADHILNLQSFPTPGET RBSK_ECOLI 9 9 -
VFGSIVQDLVSYTDSFPRPGES CEF07A115 39 39 -
VIGSLNYDLDTFTDRLPNAGET RBSK_YEAST 5 5 -
VIGSCSMDLVVTSDKRPKAGET RBSK_BACSU 6 6 -

Motif 2 width=20
Element Seqn Id St Int Rpt
FETHAGGKGLNQAAAIGKLK RBSK_YEAST 32 5 -
FKLGSGGKGANQAVAAARLG CEF07A115 66 5 -
YQIAYGGKGANQAVAAARLG RBSK_HAEIN 34 5 -
YQVAFGGKGANQAVAAGRSG RBSK_ECOLI 36 5 -
FQTVPGGKGANQAVAAARLG RBSK_BACSU 33 5 -

Motif 3 width=14
Element Seqn Id St Int Rpt
NSIVVVKGANDDIT RBSK_BACSU 104 51 -
NRILIVEGANSKTI RBSK_YEAST 111 59 -
NSIVLASGANSHLS RBSK_HAEIN 106 52 -
NNIVVTLGANLEMS CEF07A115 138 52 -
NVIGIHAGANAALS RBSK_ECOLI 108 52 -

Motif 4 width=16
Element Seqn Id St Int Rpt
ETIDHATYLTPNEHEA RBSK_BACSU 172 54 -
TILELVDIICTNENEA CEF07A115 207 55 -
ELLSLIDIITPNETEA RBSK_HAEIN 174 54 -
ELLALVDIITPNETEA RBSK_ECOLI 176 54 -
KDWELVDLLVVNEIEG RBSK_YEAST 182 57 -

Motif 5 width=12
Element Seqn Id St Int Rpt
VLITLGSRGVWA RBSK_ECOLI 220 28 -
VVMTLGSRGVLF RBSK_YEAST 245 47 -
VMITLGAKGVFV RBSK_HAEIN 218 28 -
AIITLGGKGVLL CEF07A115 251 28 -
LFITEGKQGVRY RBSK_BACSU 207 19 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
VLGSINADHVISVPYFTKPGET RBSK_HAEIN 7 7 -
VLGSINADHILNLQSFPTPGET RBSK_ECOLI 9 9 -
VFGSIVQDLVSYTDSFPRPGES Q19133 39 39 -
VFGSINMDLIATTDRLPKPGET O86458 4 4 -
VIGSCSMDLVVTSDKRPKAGET RBSK_BACSU 6 6 -
VVGSVNMDLTFVVDALPRPGET P71913 18 18 -
VLGSMNTDLVMRTKICPSGGET RBSK_SCHPO 6 6 -
VIGSLNYDLDTFTDRLPNAGET RBSK_YEAST 5 5 -
VFGSAIIDFISYTTRLPKAGET O77425 8 8 -

Motif 2 width=20
Element Seqn Id St Int Rpt
YQIAYGGKGANQAVAAARLG RBSK_HAEIN 34 5 -
YQVAFGGKGANQAVAAGRSG RBSK_ECOLI 36 5 -
FKLGSGGKGANQAVAAARLG Q19133 66 5 -
FATAAGGKGANQALAARRAG O86458 31 5 -
FQTVPGGKGANQAVAAARLG RBSK_BACSU 33 5 -
LTRTPGGKGANQAVAAARAG P71913 45 5 -
FSTGNGGKGANQAVAVARLS RBSK_SCHPO 35 7 -
FETHAGGKGLNQAAAIGKLK RBSK_YEAST 32 5 -
FQIGYGGKGANQCVAAARQG O77425 35 5 -

Motif 3 width=14
Element Seqn Id St Int Rpt
NSIVLASGANSHLS RBSK_HAEIN 106 52 -
NVIGIHAGANAALS RBSK_ECOLI 108 52 -
NNIVVTLGANLEMS Q19133 138 52 -
NMIAVVPGANGLVT O86458 102 51 -
NSIVVVKGANDDIT RBSK_BACSU 104 51 -
NTVLVAPGANAHLT P71913 116 51 -
NRILLSEGANGNVD RBSK_SCHPO 111 56 -
NRILIVEGANSKTI RBSK_YEAST 111 59 -
NNIIIVVGANNRLS O77425 107 52 -

Motif 4 width=16
Element Seqn Id St Int Rpt
ELLSLIDIITPNETEA RBSK_HAEIN 174 54 -
ELLALVDIITPNETEA RBSK_ECOLI 176 54 -
TILELVDIICTNENEA Q19133 207 55 -
RLGRLADIVIANETEF O86458 170 54 -
ETIDHATYLTPNEHEA RBSK_BACSU 172 54 -
DLAAIADVVIANEHEA P71913 182 52 -
DMISYCAYLVPNEHEA RBSK_SCHPO 179 54 -
KDWELVDLLVVNEIEG RBSK_YEAST 182 57 -
ELLQLASIFCVNESEA O77425 173 52 -

Motif 5 width=12
Element Seqn Id St Int Rpt
VMITLGAKGVFV RBSK_HAEIN 218 28 -
VLITLGSRGVWA RBSK_ECOLI 220 28 -
AIITLGGKGVLL Q19133 251 28 -
LIVTLGGDGVIA O86458 214 28 -
LFITEGKQGVRY RBSK_BACSU 207 19 -
FVITLGVRGARY P71913 207 9 -
VIITLGSQGAYY RBSK_SCHPO 226 31 -
VVMTLGSRGVLF RBSK_YEAST 245 47 -
VIITLGKLGAVF O77425 218 29 -