SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00985

Identifier
TRNASYNTHLEU  [View Relations]  [View Alignment]  
Accession
PR00985
No. of Motifs
7
Creation Date
17-SEP-1998  (UPDATE 07-JUN-1999)
Title
Leucyl-tRNA synthetase signature
Database References
PRINTS; PR90000 TRNASYNTH; PR90001 TRNASYNTHCLI
PROSITE; PS00178 AA_TRNA_LIGASE_I
PFAM; PF00749 TRNA-SYNT_1
INTERPRO; IPR002302
Literature References
1. LODISH, H., BALTIMORE, D., BERK, A., ZIPURSKY, S.L., MATSUDAIRA, P.
AND DARNELL, J.
Nucleic Acids, the Genetic Code, and Protein Synthesis. 
IN MOLECULAR CELL BIOLOGY, SCIENTIFIC AMERICAN BOOKS (NEW YORK), 1995,
PP.126-128
 
2. DELARUE, M.
Aminoacyl-tRNA synthetases.
CURR.OPIN.STRUCT.BIOL. 5 48-55 (1995).
 
3. PERONA, J.J., ROULD, M. AND STEITZ, T.A.
Structural Basis for Transfer RNA Aminoacylation by Escherichia coli
Glutaminyl-tRNA Synthetase.
BIOCHEMISTRY 32 8758-8771 (1993).

Documentation
AATRSs catalyse a two-step reaction:
 
(1) Enzyme + amino acid + ATP ---> Enzyme(aminoacyl-AMP) + PPi
 
(2) tRNA + Enzyme(aminoacyl-AMP) ---> aminoacyl-tRNA + AMP + Enzyme
 
In the first step, they form an aminoacyl-adenylate, in which the carboxyl
of the amino acid is linked to the alpha-phosphate of ATP, by displacing
the pyrophosphate. When the correct tRNA is bound, the aminoacyl group is
transferred to the 2'- or 3'-terminal OH of the tRNA at the expense of AMP
[1].
 
Based on structural and sequence comparisons, this group of at least 20
proteins (in prokaryotes there are approximately 20, but in eukaryotes
there are usually 2 forms for each amino acid; namely, the cytosolic and
mitochondrial forms) can be divided into two classes.
 
Class I AATRSs contain a characteristic Rossman fold and are mostly
monomeric. At the primary structure level, two highly-conserved motifs
are observed, `HIGH' and `KMSKS' [2,3]; these are associated with the ATP-
binding site.
 
Class II AATRSs share an anti-parallel beta-sheet formation, flanked by
alpha-helices [3], and are mostly dimeric or multimeric.
 
Further distinction between the two classes is evident when the reaction
mechanisms are investigated. In reactions catalysed by the class I AATRSs,
the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in
class II reactions, the 3'-hydroxyl site is preferred.
 
Leucyl-tRNA synthetase (EC 6.1.1.4 ) is specific to leucine and belongs
to class I.
 
TRNASYNTHLEU is a 7-element fingerprint that provides a signature for
leucyl-tRNA synthetases. The fingerprint was derived from an initial 
alignment of 10 sequences: the motifs were drawn from conserved regions
within the C-terminal portion of the alignment. Two iterations on 
OWL30.2 were required to reach convergence, at which point a true set 
comprising 20 sequences was identified. Several partial matches were also 
found: these include a C.elegans cosmid sequence that fails to match motif
4; a leucyl AATRS fragment; and two closely related valyl AATRSs.
 
An update on SPTR37_9f identified a true set of 19 sequences.
Summary Information
19 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
719191919191919
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
O51267        O66680        O84211        Q23511        
SYLM_HUMAN SYLM_NEUCR SYLM_SACDO SYLM_SCHPO
SYLM_YEAST SYL_BACSU SYL_ECOLI SYL_HAEIN
SYL_HELPY SYL_MYCGE SYL_MYCLE SYL_MYCPN
SYL_MYCTU SYL_SYNY3 SYL_TREPA
Sequence Titles
O51267      LEUCYL-TRNA SYNTHETASE (LEUS) - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE). 
O66680 LEUCYL-TRNA SYNTHETASE ALPHA SUBUNIT - AQUIFEX AEOLICUS.
O84211 LEUCYL TRNA SYNTHETASE - CHLAMYDIA TRACHOMATIS.
Q23511 ZK524.3 PROTEIN - CAENORHABDITIS ELEGANS.
SYLM_HUMAN PROBABLE LEUCYL-TRNA SYNTHETASE, MITOCHONDRIAL PRECURSOR (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) (KIAA0028) - HOMO SAPIENS (HUMAN).
SYLM_NEUCR LEUCYL-TRNA SYNTHETASE, MITOCHONDRIAL PRECURSOR (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - NEUROSPORA CRASSA.
SYLM_SACDO LEUCYL-TRNA SYNTHETASE, MITOCHONDRIAL PRECURSOR (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - SACCHAROMYCES DOUGLASII (YEAST).
SYLM_SCHPO PUTATIVE LEUCYL-TRNA SYNTHETASE, MITOCHONDRIAL PRECURSOR (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
SYLM_YEAST LEUCYL-TRNA SYNTHETASE, MITOCHONDRIAL PRECURSOR (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
SYL_BACSU LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - BACILLUS SUBTILIS.
SYL_ECOLI LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - ESCHERICHIA COLI.
SYL_HAEIN LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - HAEMOPHILUS INFLUENZAE.
SYL_HELPY LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
SYL_MYCGE LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - MYCOPLASMA GENITALIUM.
SYL_MYCLE LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - MYCOBACTERIUM LEPRAE.
SYL_MYCPN LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - MYCOPLASMA PNEUMONIAE.
SYL_MYCTU LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - MYCOBACTERIUM TUBERCULOSIS.
SYL_SYNY3 LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
SYL_TREPA LEUCYL-TRNA SYNTHETASE (EC 6.1.1.4) (LEUCINE--TRNA LIGASE) (LEURS) - TREPONEMA PALLIDUM.
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 20 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
TTDPEYYKWTQWIFLKLY SYL_BACSU 126 126 -
TCDPEYYKFTQWIFLKLF SYLM_YEAST 141 141 -
TCDPEYYKFTQWIFLKLF SYLM_SACDO 141 141 -
TTDPRYYQYTQWIFGQLF SYL_MYCPN 125 125 -
TTDPRYYKYTQWIFSELF SYL_MYCGE 125 125 -
TTDVDFYRWTQWIFLQIY SYL_MYCTU 164 164 -
TCTPEYYRWEQKFFTELY SYL_ECOLI 127 127 -
TCDPDFYKHTQKIFLALH SYLM_NEUCR 178 178 -
TCKPEYYKWEQWFFTELY SYL_HAEIN 127 127 -
TCLPDYYKWTQYLFIKLY SYLM_HUMAN 176 176 -

Motif 2 width=17
Element Seqn Id St Int Rpt
EVPVNWCPALGTVLANE SYL_BACSU 152 8 -
EAEINWDPVDMTVLANE SYLM_YEAST 167 8 -
EAEINWDPVDKTVLANE SYLM_SACDO 167 8 -
EIDVNWCAELGTVLANE SYL_MYCPN 151 8 -
DIDVNWCEQLGTVLANE SYL_MYCGE 151 8 -
DSLVNWCPGLGTVLANE SYL_MYCTU 237 55 -
TSAVNWCPNDQTVLANE SYL_ECOLI 153 8 -
EAEVNYDPVDKTVLANE SYLM_NEUCR 204 8 -
TSTVNWCPNDETVLANE SYL_HAEIN 153 8 -
EALVNWDPVDQTVLANE SYLM_HUMAN 202 8 -

Motif 3 width=14
Element Seqn Id St Int Rpt
RRLMKQWMLKINAY SYL_BACSU 184 15 -
KKQLKQWFLGITKF SYLM_YEAST 200 16 -
KKQLKQWFLGITKF SYLM_SACDO 200 16 -
KRKMKQWVLKITAF SYL_MYCPN 188 20 -
KRKMKQWVLKITTF SYL_MYCGE 188 20 -
RKRLRQWMMRITAY SYL_MYCTU 270 16 -
RKEIPQWFIKITAY SYL_ECOLI 185 15 -
KRKLKQWFLKISEF SYLM_NEUCR 237 16 -
QKEIPQWFIKITDY SYL_HAEIN 185 15 -
QKYLRQWFIKTTAY SYLM_HUMAN 235 16 -

Motif 4 width=20
Element Seqn Id St Int Rpt
SIKDMQRNWIGRSEGAHVHF SYL_BACSU 213 15 -
NVKQMQKNWIGESVGAELVF SYLM_YEAST 230 16 -
NVKQMQKNWIGESIGAELVF SYLM_SACDO 230 16 -
SIKEMQTNWIGKSEGVEVTF SYL_MYCPN 217 15 -
PIKEMQRNWIGKSKGVTINF SYL_MYCGE 217 15 -
QVKTMQRNWIGRSTGAVALF SYL_MYCTU 299 15 -
TVKTMQRNWIGRSEGVEITF SYL_ECOLI 215 16 -
RVLAMQKNWLGKSKGATVKF SYLM_NEUCR 270 19 -
MVKTMQRNWIGRSEGVEITF SYL_HAEIN 215 16 -
GIKGMQAHWIGDCVGCHLDF SYLM_HUMAN 264 15 -

Motif 5 width=19
Element Seqn Id St Int Rpt
RETNTMPQWAGSCWYFLRY SYL_BACSU 485 252 -
RETDTMDTFIDSSWYYFRF SYLM_YEAST 512 262 -
RETDTMDTFIDSSWYYFRF SYLM_SACDO 512 262 -
RETSTMPQWAGSCWYYLGY SYL_MYCPN 472 235 -
RETNTMPQWAGSCWYYLGY SYL_MYCGE 472 235 -
RDTNVMPQWAGSSWYELRY SYL_MYCTU 600 281 -
RETDTFDTFMESSWYYARY SYL_ECOLI 485 250 -
RDTDTMDTFVDSSWYYMRF SYLM_NEUCR 556 266 -
KETDTFDTFMESSWYYARY SYL_HAEIN 485 250 -
RETDTMDTFVDSAWYYFRY SYLM_HUMAN 515 231 -

Motif 6 width=23
Element Seqn Id St Int Rpt
VDMYIGGAEHAVLHLLYARFWHK SYL_BACSU 524 20 -
VDIYIGGVEHAILHLLYSRFIAK SYLM_YEAST 551 20 -
VDIYIGGVEHAILHLLYSRFIAK SYLM_SACDO 551 20 -
VDLYVGGAEHAVLHLLYARFWHQ SYL_MYCPN 517 26 -
VDLYVGGAEHAVLHLLYARFWHK SYL_MYCGE 517 26 -
VDLYVGGAEHAVLHLLYSRFWHK SYL_MYCTU 652 33 -
VDIYIGGIEHAIMHLLYFRFFHK SYL_ECOLI 524 20 -
VDLYIGGVEHAILHLLYSRFIYK SYLM_NEUCR 595 20 -
VDQYIGGIEHATMHLLYFRFFHK SYL_HAEIN 524 20 -
VDLYIGGKEHAVMHLFYARFFSH SYLM_HUMAN 554 20 -

Motif 7 width=11
Element Seqn Id St Int Rpt
KEPFQKLYNQG SYL_BACSU 557 10 -
FEPFKKLVTQG SYLM_YEAST 588 14 -
FEPFRKLVTQG SYLM_SACDO 588 14 -
KEPFKKLINQG SYL_MYCPN 550 10 -
KEPFQKLINQG SYL_MYCGE 550 10 -
REPYRRLVNQG SYL_MYCTU 685 10 -
DEPAKQLLCQG SYL_ECOLI 557 10 -
YEPFKRLITQG SYLM_NEUCR 643 25 -
EEPADKLLCQG SYL_HAEIN 557 10 -
REPFHKLLAQG SYLM_HUMAN 587 10 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
TTDPEYYKWTQWIFLKLY SYL_BACSU 126 126 -
TCDPEYYKFTQWIFLKLF SYLM_YEAST 141 141 -
TCDPEYYKFTQWIFLKLF SYLM_SACDO 141 141 -
THEENYYKWTQWIFLQLY O51267 130 130 -
TCAPDYYRWTQWLFLEFF SYL_SYNY3 127 127 -
TCDPEYYKWNQWIFLKML O66680 126 126 -
TADPEYYRWTQWLFLKLY SYL_TREPA 129 129 -
TTDPRYYQYTQWIFGQLF SYL_MYCPN 125 125 -
TTDVEFYKWTQWIFLQIY SYL_MYCLE 164 164 -
TTDPRYYKYTQWIFSELF SYL_MYCGE 125 125 -
TSDPEYYKWTQKLFLILY O84211 126 126 -
TCNPDYYKWSQYLFLQMF SYLM_SCHPO 152 152 -
TTDVDFYRWTQWIFLQIY SYL_MYCTU 164 164 -
TCTPEYYRWEQKFFTELY SYL_ECOLI 127 127 -
TCDPDFYKHTQKIFLALH SYLM_NEUCR 178 178 -
TCKPEYYKWEQWFFTELY SYL_HAEIN 127 127 -
TCLPDYYKWTQYLFIKLY SYLM_HUMAN 176 176 -
TSDPDYTKFEQRFFIDLW SYL_HELPY 123 123 -
TCEPEFFRWTQWIFCKLF Q23511 120 120 -

Motif 2 width=17
Element Seqn Id St Int Rpt
EVPVNWCPALGTVLANE SYL_BACSU 152 8 -
EAEINWDPVDMTVLANE SYLM_YEAST 167 8 -
EAEINWDPVDKTVLANE SYLM_SACDO 167 8 -
EMPVWYCPELGTVLANE O51267 156 8 -
EATVNWDPIDQTVLANE SYL_SYNY3 153 8 -
TAKVNWCPHDQTVLANE O66680 152 8 -
TAPINWCPSCKTGLANE SYL_TREPA 155 8 -
EIDVNWCAELGTVLANE SYL_MYCPN 151 8 -
DSMVNWCPGLGTVLANE SYL_MYCLE 237 55 -
DIDVNWCEQLGTVLANE SYL_MYCGE 151 8 -
DMAVNYCPELGTVLSNE O84211 152 8 -
EATVNWDPVDCTVLANE SYLM_SCHPO 178 8 -
DSLVNWCPGLGTVLANE SYL_MYCTU 237 55 -
TSAVNWCPNDQTVLANE SYL_ECOLI 153 8 -
EAEVNYDPVDKTVLANE SYLM_NEUCR 204 8 -
TSTVNWCPNDETVLANE SYL_HAEIN 153 8 -
EALVNWDPVDQTVLANE SYLM_HUMAN 202 8 -
KAMLNWCPNDKTVLANE SYL_HELPY 149 8 -
SSEVNWDPIDKTVLAAE Q23511 146 8 -

Motif 3 width=14
Element Seqn Id St Int Rpt
RRLMKQWMLKINAY SYL_BACSU 184 15 -
KKQLKQWFLGITKF SYLM_YEAST 200 16 -
KKQLKQWFLGITKF SYLM_SACDO 200 16 -
KKYLRQWVLKITKY O51267 192 19 -
RKLLRQWFLKITDY SYL_SYNY3 186 16 -
QKEVPSWFLRITAY O66680 184 15 -
RRGVRQWMVRITAY SYL_TREPA 187 15 -
KRKMKQWVLKITAF SYL_MYCPN 188 20 -
RKRLRQWMMRITAY SYL_MYCLE 270 16 -
KRKMKQWVLKITTF SYL_MYCGE 188 20 -
RRMLRQWVLRITAF O84211 184 15 -
KKNLRQWFLKISDY SYLM_SCHPO 211 16 -
RKRLRQWMMRITAY SYL_MYCTU 270 16 -
RKEIPQWFIKITAY SYL_ECOLI 185 15 -
KRKLKQWFLKISEF SYLM_NEUCR 237 16 -
QKEIPQWFIKITDY SYL_HAEIN 185 15 -
QKYLRQWFIKTTAY SYLM_HUMAN 235 16 -
QKELYQYYLKITNY SYL_HELPY 181 15 -
KKKLSQWMIETPKY Q23511 179 16 -

Motif 4 width=20
Element Seqn Id St Int Rpt
SIKDMQRNWIGRSEGAHVHF SYL_BACSU 213 15 -
NVKQMQKNWIGESVGAELVF SYLM_YEAST 230 16 -
NVKQMQKNWIGESIGAELVF SYLM_SACDO 230 16 -
SVKEMQRNWIGKSTGVEIEF O51267 221 15 -
RVKLMQSHWIGKSVGAYLEF SYL_SYNY3 216 16 -
RVIAQQRNWIGRSEGALIRF O66680 215 17 -
SVKQMQRNWIGKSCGAEIDF SYL_TREPA 216 15 -
SIKEMQTNWIGKSEGVEVTF SYL_MYCPN 217 15 -
QVKTMQRNWIGRSSGATVLF SYL_MYCLE 299 15 -
PIKEMQRNWIGKSKGVTINF SYL_MYCGE 217 15 -
SVKQLQKNWIGKSSGASVNF O84211 213 15 -
KVKKMQRNWIGRTTGFEISF SYLM_SCHPO 241 16 -
QVKTMQRNWIGRSTGAVALF SYL_MYCTU 299 15 -
TVKTMQRNWIGRSEGVEITF SYL_ECOLI 215 16 -
RVLAMQKNWLGKSKGATVKF SYLM_NEUCR 270 19 -
MVKTMQRNWIGRSEGVEITF SYL_HAEIN 215 16 -
GIKGMQAHWIGDCVGCHLDF SYLM_HUMAN 264 15 -
QVLIMQKNWIGKSSGLQFGF SYL_HELPY 212 17 -
EVADIQANWIGKCDVYRFML Q23511 209 16 -

Motif 5 width=19
Element Seqn Id St Int Rpt
RETNTMPQWAGSCWYFLRY SYL_BACSU 485 252 -
RETDTMDTFIDSSWYYFRF SYLM_YEAST 512 262 -
RETDTMDTFIDSSWYYFRF SYLM_SACDO 512 262 -
RETNTMPQWAGSCWYYLRY O51267 493 252 -
RETDTMDTFIDSSWYFLRY SYL_SYNY3 492 256 -
RETDTMDTFFDSSWYFLRF O66680 513 278 -
RETNTMPQWAGSCWYYLRY SYL_TREPA 509 273 -
RETSTMPQWAGSCWYYLGY SYL_MYCPN 472 235 -
RDTNVMPQWAGSSWYELRY SYL_MYCLE 602 283 -
RETNTMPQWAGSCWYYLGY SYL_MYCGE 472 235 -
RETHTMPQWAGSCWYYLRF O84211 477 244 -
RETDTMDTFVDSSWYYFRF SYLM_SCHPO 498 237 -
RDTNVMPQWAGSSWYELRY SYL_MYCTU 600 281 -
RETDTFDTFMESSWYYARY SYL_ECOLI 485 250 -
RDTDTMDTFVDSSWYYMRF SYLM_NEUCR 556 266 -
KETDTFDTFMESSWYYARY SYL_HAEIN 485 250 -
RETDTMDTFVDSAWYYFRY SYLM_HUMAN 515 231 -
RETDTMDTFIQSSWYFLRY SYL_HELPY 480 248 -
FDTDTLDTFFDSAWYYLRY Q23511 349 120 -

Motif 6 width=23
Element Seqn Id St Int Rpt
VDMYIGGAEHAVLHLLYARFWHK SYL_BACSU 524 20 -
VDIYIGGVEHAILHLLYSRFIAK SYLM_YEAST 551 20 -
VDIYIGGVEHAILHLLYSRFIAK SYLM_SACDO 551 20 -
VDLYIGGAEHTVLHLLYSRFWHK O51267 532 20 -
VDQYVGGIEHAILHLLYSRFFTK SYL_SYNY3 531 20 -
VDVYIGGIEHAVLHLLYARFFQK O66680 552 20 -
VALYVGGAEHAVLHLLYARFWHK SYL_TREPA 548 20 -
VDLYVGGAEHAVLHLLYARFWHQ SYL_MYCPN 517 26 -
VDLYVGGAEHAVLHLLYARFWHK SYL_MYCLE 654 33 -
VDLYVGGAEHAVLHLLYARFWHK SYL_MYCGE 517 26 -
VDLYIGGAEHAVLHLLYARFWHQ O84211 516 20 -
VDIYIGGVEHSILHLLYSRFFSK SYLM_SCHPO 537 20 -
VDLYVGGAEHAVLHLLYSRFWHK SYL_MYCTU 652 33 -
VDIYIGGIEHAIMHLLYFRFFHK SYL_ECOLI 524 20 -
VDLYIGGVEHAILHLLYSRFIYK SYLM_NEUCR 595 20 -
VDQYIGGIEHATMHLLYFRFFHK SYL_HAEIN 524 20 -
VDLYIGGKEHAVMHLFYARFFSH SYLM_HUMAN 554 20 -
VDTYIGGIEHAILHLLYARFFTK SYL_HELPY 521 22 -
VDVYVGGIEHAAVHMFFARFVAY Q23511 387 19 -

Motif 7 width=11
Element Seqn Id St Int Rpt
KEPFQKLYNQG SYL_BACSU 557 10 -
FEPFKKLVTQG SYLM_YEAST 588 14 -
FEPFRKLVTQG SYLM_SACDO 588 14 -
KEPFKKLINQG O51267 565 10 -
KEPFQKLLTQG SYL_SYNY3 564 10 -
DEPFEKLITQG O66680 585 10 -
KEPFARLVNQG SYL_TREPA 581 10 -
KEPFKKLINQG SYL_MYCPN 550 10 -
REPYRRLINQG SYL_MYCLE 687 10 -
KEPFQKLINQG SYL_MYCGE 550 10 -
PEPFKKLVNQG O84211 549 10 -
NEPFTQLITQG SYLM_SCHPO 574 14 -
REPYRRLVNQG SYL_MYCTU 685 10 -
DEPAKQLLCQG SYL_ECOLI 557 10 -
YEPFKRLITQG SYLM_NEUCR 643 25 -
EEPADKLLCQG SYL_HAEIN 557 10 -
REPFHKLLAQG SYLM_HUMAN 587 10 -
DEPFKQLITQG SYL_HELPY 554 10 -
AEPFTDLIPQG Q23511 420 10 -