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PR00959

Identifier
MEVGALKINASE  [View Relations]  [View Alignment]  
Accession
PR00959
No. of Motifs
4
Creation Date
17-AUG-1998  (UPDATE 21-JUN-1999)
Title
Mevalonate kinase family signature
Database References

PROSITE; PS00627 GHMP_KINASES_ATP
INTERPRO; IPR001459
Literature References
1. TSAY, Y.H. AND ROBINSON, G.W.
Cloning and characterization of ERG8, an essential gene of Saccharomyces
cerevisiae that encodes phosphomevalonate kinase.
MOL.CELL.BIOL. 11 620-631 (1991).

Documentation
Saccharomyces cerevisiae strains containing the erg8-1 mutation are 
temperature sensitive for growth due to a defect in phosphomevalonate 
kinase, an enzyme of isoprene and ergosterol biosynthesis [1]. Subcloning
and DNA sequencing have defined the functional ERG8 regulon as an 850bp 
upstream region and an adjacent 1,272bp open reading frame [1]. The deduced 
ERG8 protein contains 424 residues and shows no similarity to known proteins,
except within a putative ATP-binding domain present in many kinases [1].
Disruption studies have shown that the ERG8 gene is essential [1]. Its
expression in S.cerevisiae from the galactose-inducible galactokinase (GAL1)
promoter results in 1,000-fold-elevated levels of phosphomevalonate kinase
enzyme activity. 
 
Enzymes that share the N-terminal Gly/Ser-rich putative ATP-binding region
include: galactokinase, homoserine kinase, mevalonate kinase and phospho-
mevalonate kinase. 
 
MEVGALKINASE is a 4-element fingerprint that provides a signature for
mevalonate and galactokinases. The fingerprint was derived from an initial
alignment of 11 sequences: the motifs were drawn from short conserved 
regions spanning virtually the full alignment length - motif 2 includes the
region encoded by PROSITE pattern GHMP_KINASES_ATP (PS00627), which
describes the putative ATP-binding domain. Two iterations on OWL30.2 were
required to reach convergence, at which point a true set comprising 38
sequences was identified. Several partial matches were also found, all of
which are fragments or homologues that fail to match one or more motifs.
 
An update on SPTR37_9f identified a true set of 34 sequences, and 3
partial matches.
Summary Information
  34 codes involving  4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
434343434
31101
20000
1234
True Positives
GAL1_ACTPL    GAL1_BACSU    GAL1_CANAL    GAL1_CANPA    
GAL1_ECOLI GAL1_HAEIN GAL1_HUMAN GAL1_KLULA
GAL1_LACHE GAL1_SALTY GAL1_STRLI GAL1_STRMU
GAL1_YEAST GAL2_HUMAN GAL3_YEAST KIME_ARATH
KIME_HUMAN KIME_METJA KIME_METTH KIME_RAT
KIME_SCHPO KIME_YEAST O01969 O21998
O23461 O23723 O24021 O27995
O48936 O51631 O58107 O59291
O84902 O85253
True Positive Partials
Codes involving 3 elements
O83433
Sequence Titles
GAL1_ACTPL  GALACTOKINASE (EC 2.7.1.6) - ACTINOBACILLUS PLEUROPNEUMONIAE (HAEMOPHILUS PLEUROPNEUMONIAE). 
GAL1_BACSU GALACTOKINASE (EC 2.7.1.6) - BACILLUS SUBTILIS.
GAL1_CANAL GALACTOKINASE (EC 2.7.1.6) - CANDIDA ALBICANS (YEAST).
GAL1_CANPA GALACTOKINASE (EC 2.7.1.6) - CANDIDA PARAPSILOSIS (YEAST).
GAL1_ECOLI GALACTOKINASE (EC 2.7.1.6) - ESCHERICHIA COLI.
GAL1_HAEIN GALACTOKINASE (EC 2.7.1.6) - HAEMOPHILUS INFLUENZAE.
GAL1_HUMAN GALACTOKINASE 1 (EC 2.7.1.6) - HOMO SAPIENS (HUMAN).
GAL1_KLULA GALACTOKINASE (EC 2.7.1.6) - KLUYVEROMYCES LACTIS (YEAST).
GAL1_LACHE GALACTOKINASE (EC 2.7.1.6) - LACTOBACILLUS HELVETICUS.
GAL1_SALTY GALACTOKINASE (EC 2.7.1.6) - SALMONELLA TYPHIMURIUM.
GAL1_STRLI GALACTOKINASE (EC 2.7.1.6) - STREPTOMYCES LIVIDANS.
GAL1_STRMU GALACTOKINASE (EC 2.7.1.6) - STREPTOCOCCUS MUTANS.
GAL1_YEAST GALACTOKINASE (EC 2.7.1.6) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
GAL2_HUMAN GALACTOKINASE 2 (EC 2.7.1.6) - HOMO SAPIENS (HUMAN).
GAL3_YEAST GAL3 PROTEIN - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
KIME_ARATH MEVALONATE KINASE (EC 2.7.1.36) (MK) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
KIME_HUMAN MEVALONATE KINASE (EC 2.7.1.36) (MK) - HOMO SAPIENS (HUMAN).
KIME_METJA MEVALONATE KINASE (EC 2.7.1.36) (MK) - METHANOCOCCUS JANNASCHII.
KIME_METTH MEVALONATE KINASE (EC 2.7.1.36) (MK) - METHANOBACTERIUM THERMOAUTOTROPHICUM.
KIME_RAT MEVALONATE KINASE (EC 2.7.1.36) (MK) - RATTUS NORVEGICUS (RAT).
KIME_SCHPO PUTATIVE MEVALONATE KINASE (EC 2.7.1.36) (MK) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
KIME_YEAST MEVALONATE KINASE (EC 2.7.1.36) (MVK) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
O01969 SIMILAR TO GALACTOKINASE - CAENORHABDITIS ELEGANS.
O21998 GALACTOKINASE - BACTERIOPHAGE LAMBDA.
O23461 HYPOTHETICAL PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23723 PUTATIVE ARABINOSE KINASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O24021 GALACTOKINASE (EC 2.7.1.6) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O27995 MEVALONATE KINASE (MVK) - ARCHAEOGLOBUS FULGIDUS.
O48936 GALACTOKINASE (EC 2.7.1.6) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O51631 MEVALONATE KINASE - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
O58107 350AA LONG HYPOTHETICAL GALACTOKINASE - PYROCOCCUS HORIKOSHII.
O59291 335AA LONG HYPOTHETICAL PROTEIN - PYROCOCCUS HORIKOSHII.
O84902 GALACTOKINASE (EC 2.7.1.6) - LACTOBACILLUS CASEI.
O85253 GALACTOKINASE (EC 2.7.1.6) - THERMOTOGA NEAPOLITANA.

O83433 GALACTOKINASE - TREPONEMA PALLIDUM.
Scan History
OWL30_2    2  100  NSINGLE    
SPTR37_9f 2 70 NSINGLE
Initial Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
APGRVNLIGEHTDYNDGFVLPCAID GAL1_ECOLI 24 24 -
APGRVNLIGEHTDYNGGHVFPCALT GAL1_BACSU 24 24 -
SPGRVNLIGEHIDYCDFSVLPLAID GAL3_YEAST 44 44 -
APGRVNIIGEHIDYCGYSVLPMAVE GAL2_HUMAN 40 40 -
APGKVILHGEHAVVHGKVALAVALN KIME_RAT 10 10 -
APGKIILAGEHAVVHGSTAVAAAID KIME_ARATH 7 7 -
APGKVILHGEHAVVHGKVALAVSLN KIME_HUMAN 10 10 -
SPGKTILFGEHAVVYGATALAAAVS KIME_SCHPO 9 9 -
APGKVIIFGEHSAVYNKPAVAASVS KIME_YEAST 9 9 -
APAKAILFGEHAVVYSKPAIAAAID KIME_METTH 7 7 -
TPSKVILFGEHAVVYGYRAISMAID KIME_METJA 5 5 -

Motif 2 width=23
Element Seqn Id St Int Rpt
GNVPQGAGLSSSASLEVAVGTVL GAL1_ECOLI 118 69 -
GNIPNGAGLSSSASIELLMGVVL GAL1_BACSU 119 70 -
SDIPTGGGLSSAFTCAAALATIR GAL3_YEAST 154 85 -
GNIPPSSGLSSSSALVCCAGLVT GAL2_HUMAN 136 71 -
SELPPGAGLGSSAAYSVCVAAAL KIME_RAT 135 100 -
SELPYGSGLGSSAALCVALTAAL KIME_ARATH 138 106 -
SELPPGAGLGSSAAYSVCLAAAL KIME_HUMAN 135 100 -
SQVPLGAGLGSSATISVVVATSL KIME_SCHPO 130 96 -
STLPIGAGLGSSASISVSLALAM KIME_YEAST 138 104 -
MEIPAGSGLGSSAALTVALIGAL KIME_METTH 87 55 -
SKIPISCGLGSSASITIGTIKAV KIME_METJA 101 71 -

Motif 3 width=20
Element Seqn Id St Int Rpt
ENQFVGCNCGIMDQLISALG GAL1_ECOLI 161 20 -
ENHFIGVNCGIMDQFAIGMG GAL1_BACSU 162 20 -
AEHYVGVNNGGMDQATSVYG GAL3_YEAST 197 20 -
SERYIGTEGGGMDQSISFLA GAL2_HUMAN 178 19 -
ERVIHGNPSGVDNSVSTWGG KIME_RAT 193 35 -
EKIIHGKPSGIDNTVSAYGN KIME_ARATH 193 32 -
ERMIHGNPSGVDNAVSTWGG KIME_HUMAN 193 35 -
ECCIHGTPSGIDNAVATNGG KIME_SCHPO 184 31 -
EKCIHGTPSGIDNAVATYGN KIME_YEAST 191 30 -
EVDVQGAASPLDTAISTYGG KIME_METTH 130 20 -
EKEIQGKASITDTSTITYKG KIME_METJA 144 20 -

Motif 4 width=18
Element Seqn Id St Int Rpt
GVRMTGGGFGGCIVALIP GAL1_ECOLI 327 146 -
GSRMTGAGFGGCTISIVK GAL1_BACSU 333 151 -
GSRLTGAGWGGCTIHLVP GAL3_YEAST 454 237 -
GSRLTGAGWGGCTVSMVP GAL2_HUMAN 396 198 -
HSKLTGAGGGGCGITLLK KIME_RAT 328 115 -
VSKLTGAGGGGCVLTLLP KIME_ARATH 328 115 -
HSKLTGAGGGGCGITLLK KIME_HUMAN 328 115 -
WTKLTGAGGGGCTITLLT KIME_SCHPO 322 118 -
STKLTGAGGGGCSLTLLR KIME_YEAST 346 135 -
GSKITGAGGGGSIIAHCP KIME_METTH 259 109 -
GAKLTGAGGGGCVIILVN KIME_METJA 270 106 -
Final Motifs
Motif 1  width=25
Element Seqn Id St Int Rpt
APGRVNLIGEHTDYNDGFVLPCAID O21998 25 25 -
APGRVNLIGEHTDYNDGFVLPCAID GAL1_ECOLI 24 24 -
APGRVNLIGEHTDYNDGFVLPCAID GAL1_SALTY 25 25 -
APGRVNIIGEHTDYNDGFVMPCAIN GAL1_HAEIN 25 25 -
SPGRVNLIGEHIDYCDFSVLPLAID GAL1_YEAST 49 49 -
APGRINLIGEHTDYNGGHVFPCAIS O84902 24 24 -
APGRVNLIGEHTDYNGGHVFPCALT GAL1_BACSU 24 24 -
APGRVNLIGEHTDYNQGLVLPMALE GAL1_HUMAN 34 34 -
APGRVNIIGEHTDYNDGFVMPCAIN GAL1_ACTPL 25 25 -
SPGRVNLIGEHIDYCQFSVLPMAIE GAL1_KLULA 40 40 -
SPGRVNLIGEHTDYTYGYVMPMAIN O58107 6 6 -
SPGRVNLIGEHIDYEGYSVLPMAIR O48936 53 53 -
SPGRVNLIGEHIDYEGYSVLPMAIR O24021 53 53 -
SPGRVNLIGDHIDYNFFPVLPMAIS GAL1_CANAL 45 45 -
SPGRINLIGEHTDYNGGRVFPAAIT GAL1_STRMU 24 24 -
APGRINIIGEHTDYNDGYVLPFAVN O85253 5 5 -
SPGRVNLIGDHIDYNYFPVLPMAIE GAL1_CANPA 44 44 -
SPGRVNLIGEHIDYCDFSVLPLAID GAL3_YEAST 44 44 -
SPGRINVIGEHTDYNGGHVFPAPIS GAL1_LACHE 24 24 -
RAGRENLIGEHTDYNDGFVMPSALP GAL1_STRLI 27 27 -
APGRVNIIGEHIDYCGYSVLPMAVE GAL2_HUMAN 40 40 -
APGKVILHGEHAVVHGKVALAVALN KIME_RAT 10 10 -
APAKVILFGEHSVVYGKPAIASAIE O59291 9 9 -
APGKIILAGEHAVVHGSTAVAAAID KIME_ARATH 7 7 -
APGKVILHGEHAVVHGKVALAVSLN KIME_HUMAN 10 10 -
CAATVTFPGEHIDYHDYGVFPMAID O01969 7 7 -
APGRLDVMGGIADYSGSLVLQMPIR O23461 554 554 -
APGRLDVMGGIADYSGSLVLQMPIR O23723 504 504 -
SPGKTILFGEHAVVYGATALAAAVS KIME_SCHPO 9 9 -
APGKIILFGEHAVVYGRHAVVSAIN O27995 5 5 -
APGKVIIFGEHSAVYNKPAVAASVS KIME_YEAST 9 9 -
APAKAILFGEHAVVYSKPAIAAAID KIME_METTH 7 7 -
TPSKVILFGEHAVVYGYRAISMAID KIME_METJA 5 5 -
KPAKILFLGEHSAVYGFPVIGATVP O51631 6 6 -

Motif 2 width=23
Element Seqn Id St Int Rpt
GNVPQGAGLSSSASLEVAVGTVL O21998 119 69 -
GNVPQGAGLSSSASLEVAVGTVL GAL1_ECOLI 118 69 -
SNVPQGAGLSSSASLEVAVGTVF GAL1_SALTY 118 68 -
GNVPLSSGLSSSAALEVAVGKFC GAL1_HAEIN 118 68 -
GDVPTGSGLSSSAAFICAVALAV GAL1_YEAST 159 85 -
GNLPDGAGLSSSASLEMLVGTIL O84902 118 69 -
GNIPNGAGLSSSASIELLMGVVL GAL1_BACSU 119 70 -
SSVPLGGGLSSSASLEVATYTFL GAL1_HUMAN 131 72 -
SDVPMSSGLSSSAALEISIGKTA GAL1_ACTPL 118 68 -
GDIPSGGGLSSSAAFICAVSLAI GAL1_KLULA 148 83 -
GNLPLGAGLSSSASFEVGILETL O58107 94 63 -
GIVPTGSGLSSSAAFVCSATIAI O48936 156 78 -
GIVPTGSGLSSSAAFVCSATIAI O24021 156 78 -
GNVPTGGGLSSSAAFCVASTLAI GAL1_CANAL 150 80 -
GNIPNGSGLSSSSSLELLIGIMA GAL1_STRMU 119 70 -
SNLPMGAGLSSSAALEMATAYAI O85253 91 61 -
GTVPTGGGLSSSAAFCVASTLAV GAL1_CANPA 145 76 -
SDIPTGGGLSSAFTCAAALATIR GAL3_YEAST 154 85 -
ANLPSGSGLSSSAAIEMLMGIIL GAL1_LACHE 120 71 -
STVPSGAGLSSSAALEVRPLAMN GAL1_STRLI 119 67 -
GNIPPSSGLSSSSALVCCAGLVT GAL2_HUMAN 136 71 -
SELPPGAGLGSSAAYSVCVAAAL KIME_RAT 135 100 -
SQIPVGAGLGSSAAVAVATIGAV O59291 108 74 -
SELPYGSGLGSSAALCVALTAAL KIME_ARATH 138 106 -
SELPPGAGLGSSAAYSVCLAAAL KIME_HUMAN 135 100 -
GTIPPSSGLSSSSSLVCASALAT O01969 103 71 -
SAVPEGKGVSSSAAVEVASMSAI O23461 690 111 -
SAVPEGKGVSSSAAVEVASMSAI O23723 640 111 -
SQVPLGAGLGSSATISVVVATSL KIME_SCHPO 130 96 -
SEIPIGSGLGSSAAVIVATIAAL O27995 83 53 -
STLPIGAGLGSSASISVSLALAM KIME_YEAST 138 104 -
MEIPAGSGLGSSAALTVALIGAL KIME_METTH 87 55 -
SKIPISCGLGSSASITIGTIKAV KIME_METJA 101 71 -
SEIPIGVGLGSSASLSLCFAEYI O51631 76 45 -

Motif 3 width=20
Element Seqn Id St Int Rpt
ENQFVGCNCGIMDQLISALG O21998 162 20 -
ENQFVGCNCGIMDQLISALG GAL1_ECOLI 161 20 -
ENQFVGCNCGIMDQLISALG GAL1_SALTY 160 19 -
ENQFVGANCGNMDQLISALG GAL1_HAEIN 161 20 -
AEHYVGVNNGGMDQAASVCG GAL1_YEAST 204 22 -
ENDYIGVNSGVMDQFAIEMG O84902 161 20 -
ENHFIGVNCGIMDQFAIGMG GAL1_BACSU 162 20 -
EHSFAGMPCGIMDQFISLMG GAL1_HUMAN 174 20 -
ENKFVGANCGNMDQLTSALG GAL1_ACTPL 161 20 -
AEHHVGVNNGGMDQAASICG GAL1_KLULA 193 22 -
ENEFVGVPCGILDQFAVVFG O58107 137 20 -
CERHIGTQSGGMDQAISIMA O48936 198 19 -
CERHIGTQSGGMDQAISIMA O24021 198 19 -
CEHYVGVNTGGMDQCASVYG GAL1_CANAL 193 20 -
ENDFIGVNSGIMDQFAIGMG GAL1_STRMU 162 20 -
EVEFVGVRCGIMDQFTSAFG O85253 134 20 -
SEHYLGLNNGGMDQCASVYG GAL1_CANPA 188 20 -
AEHYVGVNNGGMDQATSVYG GAL3_YEAST 197 20 -
ENEFIGLNSGIMDQFACIMG GAL1_LACHE 163 20 -
ENVYVGAPVGIMDQTASACC GAL1_STRLI 161 19 -
SERYIGTEGGGMDQSISFLA GAL2_HUMAN 178 19 -
ERVIHGNPSGVDNSVSTWGG KIME_RAT 193 35 -
ELLVQGASSGIDPTVSAVGG O59291 151 20 -
EKIIHGKPSGIDNTVSAYGN KIME_ARATH 193 32 -
ERMIHGNPSGVDNAVSTWGG KIME_HUMAN 193 35 -
SEPLIGTLSGGMDQAAEVLA O01969 172 46 -
ENHIVGAPCGVMDQMTSSCG O23461 733 20 -
ENHIVGAPCGVMDQMTSSCG O23723 683 20 -
ECCIHGTPSGIDNAVATNGG KIME_SCHPO 184 31 -
EIDVQGRASGIDPFISTFGG O27995 126 20 -
EKCIHGTPSGIDNAVATYGN KIME_YEAST 191 30 -
EVDVQGAASPLDTAISTYGG KIME_METTH 130 20 -
EKEIQGKASITDTSTITYKG KIME_METJA 144 20 -
ENIFHGKSSGMDIRLIDLNG O51631 117 18 -

Motif 4 width=18
Element Seqn Id St Int Rpt
GVRMTGGGFGGCIVALIP O21998 328 146 -
GVRMTGGGFGGCIVALIP GAL1_ECOLI 327 146 -
GVRMTGGGFGGCVVALIP GAL1_SALTY 326 146 -
GARMTGGGFGGCIVALAP GAL1_HAEIN 330 149 -
GSRLTGAGWGGCTVHLVP GAL1_YEAST 461 237 -
GARMTGAGFGGCAIAIVN O84902 332 151 -
GSRMTGAGFGGCTISIVK GAL1_BACSU 333 151 -
GSRMTGGGFGGCTVTLLE GAL1_HUMAN 340 146 -
GARMTGGGFGGCIVCLVP GAL1_ACTPL 330 149 -
GSRLTGAGWGGCTVHLCS GAL1_KLULA 441 228 -
GARLTGAGFGGSAIALVD O58107 297 140 -
GARLTGAGWGGCAVALVK O48936 431 213 -
GARLTGAGWGGCAVALVK O24021 432 214 -
GSRITGAGWGGSIVHLTT GAL1_CANAL 447 234 -
GARMTGSGFGGCGIAIVA GAL1_STRMU 333 151 -
GARMVGGGFGGGVIVLSK O85253 296 142 -
GSRVTGAGWGGSIVHLTT GAL1_CANPA 434 226 -
GSRLTGAGWGGCTIHLVP GAL3_YEAST 454 237 -
GARMIGGGFGGSAIAIVK GAL1_LACHE 334 151 -
GRRMTGGGFGGSAIVLVE GAL1_STRLI 330 149 -
GSRLTGAGWGGCTVSMVP GAL2_HUMAN 396 198 -
HSKLTGAGGGGCGITLLK KIME_RAT 328 115 -
GAKLTGAGGGGCMYALAP O59291 284 113 -
VSKLTGAGGGGCVLTLLP KIME_ARATH 328 115 -
HSKLTGAGGGGCGITLLK KIME_HUMAN 328 115 -
GARLTGAGWGGCAVVLMA O01969 381 189 -
GAKITGGGSGGTVCVVGR O23461 974 221 -
GAKITGGGSGGTVCVVGR O23723 924 221 -
WTKLTGAGGGGCTITLLT KIME_SCHPO 322 118 -
NAKITGAGGGGCIFGLFK O27995 246 100 -
STKLTGAGGGGCSLTLLR KIME_YEAST 346 135 -
GSKITGAGGGGSIIAHCP KIME_METTH 259 109 -
GAKLTGAGGGGCVIILVN KIME_METJA 270 106 -
SGKLSGAGKGGAFIFLFE O51631 248 111 -