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PR00954

Identifier
FLGMOTORFLIG  [View Relations]  [View Alignment]  
Accession
PR00954
No. of Motifs
7
Creation Date
18-AUG-1998  (UPDATE 07-JUN-1999)
Title
Flagellar motor switch protein FliG signature
Database References

INTERPRO; IPR000090
Literature References
1. ROMAN, S.J., FRANTZ, B.B. AND MATSUMURA, P.
Gene sequence, overproduction, purification and determination of the
wild-type level of the Escherichia coli flagellar switch protein FliG. 
GENE 133 103-108 (1993). 
 
2. MARYKWAS, D.L. AND BERG, H.C.
A mutational analysis of the interaction between FliG and FliM, two
components of the flagellar motor of Escherichia coli. 
J.BACTERIOL. 178 1289-1294 (1996). 
 
3. KIHARA, M., HOMMA, M., KUTSUKAKE, K. AND MACNAB, R.M.
Flagellar switch of Salmonella typhimurium: gene sequences and deduced 
protein sequences. 
J.BACTERIOL. 171 3247-3257 (1989). 
 
4. FRANCIS, N.R., IRIKURA, V.M., YAMAGUCHI, S., DEROSIER, D.J.
AND MACNAB, R.M.
Localization of the Salmonella typhimurium flagellar switch protein FliG to
the cytoplasmic M-ring face of the basal body. 
PROC.NATL.ACAD.SCI.U.S.A. 89 6304-6308 (1992). 

Documentation
The flagellar motor switch in E.coli and S.typhimurium regulates the 
direction of flagellar rotation and hence controls swimming behaviour [1].
The switch is a complex apparatus that responds to signals transduced by the
chemotaxis sensory signalling system during chemotactic behaviour [1]. CheY,
the chemotaxis response regulator, is believed to act directly on the switch
to induce tumbles in the swimming pattern, but no physical interactions of 
CheY and switch proteins have yet been demonstrated. 
 
The switch complex comprises at least three proteins - FliG, FliM and FliN.
It has been shown that FliG interacts with FliM, FliM interacts with itself,
and FliM interacts with FliN [2]. Several residues within the middle third
of FliG appear to be strongly involved in the FliG-FliM interaction, with
residues near the N- or C-termini being less important [2]. Such clustering
suggests that FliG-FliM interaction plays a central role in switching. 
 
Analysis of the FliG, FliM and FliN sequences shows that none are especially
hydrophobic or appear to be integral membrane proteins [3]. This result is
consistent with other evidence suggesting that the proteins may be 
peripheral to the membrane, possibly mounted on the basal body M ring [3,4]. 
 
FLGMOTORFLIG is a 7-element fingerprint that provides a signature for
flagellar motor switch FliG proteins. The fingerprint was derived from
an initial alignment of 7 sequences: the motifs were drawn from short
conserved regions spanning the full alignment length. Two iterations on
OWL30.2 were required to reach convergence, at which point a true set
comprising 12 sequences was identified. Two partial matches were also
found, both of which are fragments.
 
An update on SPTR37_9f identified a true set of 14 sequences, and 1
partial match.
Summary Information
  14 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
714141414141414
60000000
50000000
40000000
30011001
20000000
1234567
True Positives
FLIG_AGRTU    FLIG_BACSU    FLIG_BORBU    FLIG_CAUCR    
FLIG_ECOLI FLIG_SALTY O25119 O51239
O54244 O66891 O83415 Q53152
Q56263 Q57369
True Positive Partials
Codes involving 3 elements
O83070
Sequence Titles
FLIG_AGRTU  FLAGELLAR MOTOR SWITCH PROTEIN FLIG - AGROBACTERIUM TUMEFACIENS. 
FLIG_BACSU FLAGELLAR MOTOR SWITCH PROTEIN FLIG - BACILLUS SUBTILIS.
FLIG_BORBU FLAGELLAR MOTOR SWITCH PROTEIN FLIG - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
FLIG_CAUCR FLAGELLAR MOTOR SWITCH PROTEIN FLIG - CAULOBACTER CRESCENTUS.
FLIG_ECOLI FLAGELLAR MOTOR SWITCH PROTEIN FLIG - ESCHERICHIA COLI.
FLIG_SALTY FLAGELLAR MOTOR SWITCH PROTEIN FLIG - SALMONELLA TYPHIMURIUM.
O25119 FLAGELLAR MOTOR SWITCH PROTEIN (FLIG) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
O51239 FLAGELLAR MOTOR SWITCH PROTEIN (FLIG-1) - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
O54244 FLAGELLAR C-RING PROTEIN - SINORHIZOBIUM MELILOTI.
O66891 FLAGELLAR SWITCH PROTEIN FLIG - AQUIFEX AEOLICUS.
O83415 FLAGELLAR MOTOR SWITCH PROTEIN (FLIG-2) - TREPONEMA PALLIDUM.
Q53152 FLIG PROTEIN - RHODOBACTER SPHAEROIDES (RHODOPSEUDOMONAS SPHAEROIDES).
Q56263 FLAGELLAR MOTOR SWITCH PROTEIN FLIG - TREPONEMA DENTICOLA.
Q57369 FLAGELLAR SWITCH PROTEIN - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).

O83070 FLAGELLAR MOTOR SWITCH PROTEIN (FLIG-1) - TREPONEMA PALLIDUM.
Scan History
OWL30_2    2  50   NSINGLE    
SPTR37_9f 2 19 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LTGRQKAAIFLVSLGGE TDU787765 21 21 -
LSGTDKSVILLMTIGED FLIG_SALTY 4 4 -
LTGKQKAAILMISLGLD FLIG_BACSU 9 9 -
LTGKQKAAILLVSIGSE FLIG_BORBU 15 15 -
LSGPEKAAIVLLALGED FLIG_CAUCR 13 13 -
LTGTDKSVILLMTIGED FLIG_ECOLI 4 4 -
LTGKQKAAILLVSIGSE BBU09711 15 15 -

Motif 2 width=27
Element Seqn Id St Int Rpt
EVQALSTAMANVRQISNKQLTDVLSEF FLIG_SALTY 33 12 -
EVQTLSAAMANVTQISNKQLTDVLAEF FLIG_ECOLI 33 12 -
EIESLTFEIAKLETITSELKDNVLLEF BBU09711 44 12 -
EVEKIVFEIARTETVEAELKDAVLQEF TDU787765 50 12 -
EIKEVSQAMAGLGTVSASVVEDLLVEF FLIG_CAUCR 41 11 -
EIESLTFEIAKLETITSELKDNVLLEF FLIG_BORBU 44 12 -
EIERLTLEISGVRSVDHQKKDEIIEEF FLIG_BACSU 38 12 -

Motif 3 width=24
Element Seqn Id St Int Rpt
EPQSAADLIRDEHPQIIATILVHL FLIG_SALTY 114 54 -
EPQSAADLIRDEHPQIIATILVHL FLIG_ECOLI 114 54 -
NEAVLANYLKNEYPQTVAVVLSKV FLIG_CAUCR 120 52 -
DPAHLLNFIQQEHPQTIALILAYL TDU787765 130 53 -
EPEQILNFIQQEHPQTMALILSYL FLIG_BACSU 118 53 -
DPANILNFIQQEHPQTIALILSYL FLIG_BORBU 124 53 -
DPANILNFIQQEHPQTIALILSYL BBU09711 124 53 -

Motif 4 width=26
Element Seqn Id St Int Rpt
GGVDNVVEIINMADRKTEKFIIESLE BBU09711 205 57 -
GGVDSIVEILNLVDRSSEKSIIESLE TDU787765 211 57 -
GGVDNVVEIINMADRKTEKFIIESLE FLIG_BORBU 205 57 -
GGIEAVVEVLNGVDRGTEKTILDSLE FLIG_BACSU 199 57 -
GGVRTAAEIINLMKTQQEEAVITAVR FLIG_SALTY 194 56 -
GGVRTAAEIINLMKTQQEEAVITAVR FLIG_ECOLI 194 56 -
DSHEMMAEIFNNFDRQTEARFIAALE FLIG_CAUCR 200 56 -

Motif 5 width=21
Element Seqn Id St Int Rpt
LAEEIKKKMFVFEDIVMLDDR TDU787765 242 5 -
LAEEIKKKMFVFEDIVLLDDR FLIG_BORBU 236 5 -
LAEEIKKRMFVFEDIVTLDNR FLIG_BACSU 230 5 -
LAQKIIDEMFLFENLVDVDDR FLIG_SALTY 225 5 -
LAQKIIDEMFLFENLVDVDDR FLIG_ECOLI 225 5 -
AAERIRALMFVFEDLSKLDPG FLIG_CAUCR 231 5 -
LAEEIKKKMFVFEDIVLLDDR BBU09711 236 5 -

Motif 6 width=21
Element Seqn Id St Int Rpt
IQTLLRGTPKEQLALALKGAS FLIG_CAUCR 253 1 -
ISKVLREVNNDEMAKALKQVD TDU787765 264 1 -
IQRVLREIDGQELAKALKSVD BBU09711 258 1 -
IQRVLREIDGQELAKALKSVD FLIG_BORBU 258 1 -
IQRVIRDVENDDLLLSLKVAS FLIG_BACSU 252 1 -
IQRLLQEVDSESLLIALKGAE FLIG_SALTY 247 1 -
IQRLLQEVDSESLLIALKGAE FLIG_ECOLI 247 1 -

Motif 7 width=27
Element Seqn Id St Int Rpt
QDKIFRNMSKRAGAMLRDEMEYMGPIR TDU787765 288 3 -
QEKIFKNMSKRAASMLKEDMEFLGPTR BBU09711 282 3 -
QEKIFKNMSKRAASMLKEDMEFLGPTR FLIG_BORBU 282 3 -
KEIVFNNMSQRMVETFKEEMEFMGPVR FLIG_BACSU 276 3 -
REKFLRNMSQRAADILRDDLANRGPVR FLIG_SALTY 271 3 -
REKFLRNMSQRAADILRDDLANRGPVR FLIG_ECOLI 271 3 -
RDLFFSNMSERAAKIMREDMDSMGPVR FLIG_CAUCR 277 3 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LTGKQKAAILLVSIGSE FLIG_BORBU 15 15 -
LTGKQKAAILLVSIGSE Q57369 15 15 -
LNGRQKAAIFLVSIGEE O83415 23 23 -
LTGRQKAAIFLVSLGGE Q56263 21 21 -
LTGKQKAAILMISLGLD FLIG_BACSU 9 9 -
LSGTDKSVILLMTIGED FLIG_SALTY 4 4 -
LTGTDKSVILLMTIGED FLIG_ECOLI 4 4 -
LTGTQKAAILLMLFGET Q53152 12 12 -
LSGPEKAAIVLLALGED FLIG_CAUCR 13 13 -
LSKAQKAAVLLLSLPEE O66891 8 8 -
LSMSEKIAILLIQVGED O25119 16 16 -
LSQADKAAAVLLAMGKG FLIG_AGRTU 16 16 -
LSQTEKAAAVLLAMGKS O54244 18 18 -
ESKVTKIAKYFLAIGLE O51239 79 79 -

Motif 2 width=27
Element Seqn Id St Int Rpt
EIESLTFEIAKLETITSELKDNVLLEF FLIG_BORBU 44 12 -
EIESLTFEIAKLETITSELKDNVLLEF Q57369 44 12 -
EIEKLVFEIARTESVDAELKDAVLEEF O83415 52 12 -
EVEKIVFEIARTETVEAELKDAVLQEF Q56263 50 12 -
EIERLTLEISGVRSVDHQKKDEIIEEF FLIG_BACSU 38 12 -
EVQALSTAMANVRQISNKQLTDVLSEF FLIG_SALTY 33 12 -
EVQTLSAAMANVTQISNKQLTDVLAEF FLIG_ECOLI 33 12 -
EVQHLGTAMYSVRGIDQDTLSLVLEDF Q53152 41 12 -
EIKEVSQAMAGLGTVSASVVEDLLVEF FLIG_CAUCR 41 11 -
ELQKLFALAKDLESVPEEEIENIAEEL O66891 37 12 -
SITEISKQIVQLNGTDKQIGAAVLEEF O25119 45 12 -
ELQMIISSAQTLRVIPPDELAQIVAEF FLIG_AGRTU 45 12 -
ELQAIIAAAQSLRAVPPHELEALVNEF O54244 47 12 -
DIILITREISKISYIAPEEKEHIIKEF O51239 108 12 -

Motif 3 width=24
Element Seqn Id St Int Rpt
DPANILNFIQQEHPQTIALILSYL FLIG_BORBU 124 53 -
DPANILNFIQQEHPQTIALILSYL Q57369 124 53 -
DPTHLLNFIQQEHPQTIALILAYL O83415 132 53 -
DPAHLLNFIQQEHPQTIALILAYL Q56263 130 53 -
EPEQILNFIQQEHPQTMALILSYL FLIG_BACSU 118 53 -
EPQSAADLIRDEHPQIIATILVHL FLIG_SALTY 114 54 -
EPQSAADLIRDEHPQIIATILVHL FLIG_ECOLI 114 54 -
DAPSIAELLVDEHPQIMALTVACL Q53152 121 53 -
NEAVLANYLKNEYPQTVAVVLSKV FLIG_CAUCR 120 52 -
DSRILASLLKNEHPQTIALFLSQL O66891 116 52 -
KPQQLADFIINEHPQTIALILAHM O25119 126 54 -
EPEFVGKFLLREHPQTIAYILSML FLIG_AGRTU 126 54 -
DPVIIAQLLAREHPQTIAYVLSMM O54244 128 54 -
EHEQLWALIKDENIKTLVILYNYL O51239 188 53 -

Motif 4 width=26
Element Seqn Id St Int Rpt
GGVDNVVEIINMADRKTEKFIIESLE FLIG_BORBU 205 57 -
GGVDNVVEIINMADRKTEKFIIESLE Q57369 205 57 -
GGVQNIVDILNLVDRSSEKSIVEALE O83415 213 57 -
GGVDSIVEILNLVDRSSEKSIIESLE Q56263 211 57 -
GGIEAVVEVLNGVDRGTEKTILDSLE FLIG_BACSU 199 57 -
GGVRTAAEIINLMKTQQEEAVITAVR FLIG_SALTY 194 56 -
GGVRTAAEIINLMKTQQEEAVITAVR FLIG_ECOLI 194 56 -
GGVKAAARIMNFTRTATEARILKDIR Q53152 203 58 -
DSHEMMAEIFNNFDRQTEARFIAALE FLIG_CAUCR 200 56 -
EGTAVAAELLNTLDKETRELILQSIG O66891 197 57 -
GGLRAVAEIFNRLGQKSAKTTLARIE O25119 206 56 -
GGSTKVADLMNELDKPQVDTLLSSLE FLIG_AGRTU 205 55 -
PGPAKIAEVMNELEKPQVDTLLASLE O54244 207 55 -
DGSKILVDILSYMDFNEEKNLLNNIN O51239 269 57 -

Motif 5 width=21
Element Seqn Id St Int Rpt
LAEEIKKKMFVFEDIVLLDDR FLIG_BORBU 236 5 -
LAEEIKKKMFVFEDIVLLDDR Q57369 236 5 -
LAEEIKKRMFVFEDIVMLDDR O83415 244 5 -
LAEEIKKKMFVFEDIVMLDDR Q56263 242 5 -
LAEEIKKRMFVFEDIVTLDNR FLIG_BACSU 230 5 -
LAQKIIDEMFLFENLVDVDDR FLIG_SALTY 225 5 -
LAQKIIDEMFLFENLVDVDDR FLIG_ECOLI 225 5 -
LMQAIQDNMFVFDNLIKSDDR Q53152 234 5 -
AAERIRALMFVFEDLSKLDPG FLIG_CAUCR 231 5 -
LEERIREKMFTFEDIRKLSDR O66891 228 5 -
LAGAIKEMMFTFEDIVKLDNF O25119 237 5 -
AANKVKPKIFLFDDLMFMPQR FLIG_AGRTU 236 5 -
SAKKVRPKIFLFDDILFMPQR O54244 238 5 -
VDSEIREKIFDINVILRIMDN O51239 302 7 -

Motif 6 width=21
Element Seqn Id St Int Rpt
IQRVLREIDGQELAKALKSVD FLIG_BORBU 258 1 -
IQRVLREIDGQELAKALKSVD Q57369 258 1 -
IQKVLREVNMEELAKALKVVD O83415 266 1 -
ISKVLREVNNDEMAKALKQVD Q56263 264 1 -
IQRVIRDVENDDLLLSLKVAS FLIG_BACSU 252 1 -
IQRLLQEVDSESLLIALKGAE FLIG_SALTY 247 1 -
IQRLLQEVDSESLLIALKGAE FLIG_ECOLI 247 1 -
LQTLLRAVDNETLVLALKGAD Q53152 256 1 -
IQTLLRGTPKEQLALALKGAS FLIG_CAUCR 253 1 -
IIEILKVVDKNTLMIALLGAP O66891 250 1 -
IREILKVADKKDLSLALKTST O25119 259 1 -
RVLLLNDVSADVLTMALRGAT FLIG_AGRTU 258 1 -
RVQLFNDVSADVITMALRGSA O54244 260 1 -
LHNILREFTDKEIAIIIKDKA O51239 324 1 -

Motif 7 width=27
Element Seqn Id St Int Rpt
QEKIFKNMSKRAASMLKEDMEFLGPTR FLIG_BORBU 282 3 -
QEKIFKNMSKRAASMLKEDMEFLGPTR Q57369 282 3 -
QDKIFRNMSKRAGSMLKEEMEYMGPTR O83415 290 3 -
QDKIFRNMSKRAGAMLRDEMEYMGPIR Q56263 288 3 -
KEIVFNNMSQRMVETFKEEMEFMGPVR FLIG_BACSU 276 3 -
REKFLRNMSQRAADILRDDLANRGPVR FLIG_SALTY 271 3 -
REKFLRNMSQRAADILRDDLANRGPVR FLIG_ECOLI 271 3 -
RAKILGCMSTRAAATVRDEMEALGPVR Q53152 280 3 -
RDLFFSNMSERAAKIMREDMDSMGPVR FLIG_CAUCR 277 3 -
KQKFLSNMSKRAAKLFLEDMEALGPVK O66891 274 3 -
TDKFLNNMSSRAAEQFVEEMQYLGAVK O25119 283 3 -
KECVLSSISPRQRRMIESDLAVPQASL FLIG_AGRTU 282 3 -
RESILASIGARQRRMIESDLAAGDAGI O54244 284 3 -
RDKMLSNVSKRRKEIILDEEAFLGKIS O51239 348 3 -