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PR00773

Identifier
GRPEPROTEIN  [View Relations]  [View Alignment]  
Accession
PR00773
No. of Motifs
4
Creation Date
24-AUG-1997  (UPDATE 07-JUN-1999)
Title
GrpE protein signature
Database References

PROSITE; PS01071 GRPE
PFAM; PF01025 GrpE
INTERPRO; IPR000740
Literature References
1. GEORGOPOULOS, C. AND WELCH, W.
Role of the major heat-shock proteins as molecular chaperones.
ANNU.REV.CELL BIOL. 9 601-635 (1993).
 
2. BOLLIGER, L., DELOCHE, O., GLICK, B.S., GEORGOPOULOS, C., JENOE, P.,
KRONIDOU, N., HORST, M., MORISHIMA, N. AND SCHATZ, G.
A mitochondrial homolog of bacterial grpE interacts with mitochondrial
hsp70 and is essential for viability.
EMBO J. 13 1998-2006 (1994).

Documentation
Acting in concert with dnaJ, the prokaryotic grpE protein stimulates
the ATPase activity of the dnaK chaperone [1]. The protein apparently
accelerate the release of ADP from dnaK, allowing dnaK to recycle more
efficiently. An evolutionarily related mitochondrial protein in yeast has
been shown to associate with the mitochondrial hsp70 protein; it thus 
plays a role in the import of proteins from the cytoplasm [2].
 
GRPEPROTEIN is a 4-element fingerprint that provides a signature for the
grpE protein family. The fingerprint was derived from an initial alignment
of 9 sequences: the motifs were drawn from short conserved regions spanning
the full alignment length - motifs 3 and 4 span the region encoded by
PROSITE pattern GRPE (PS01071), which lies at the C-terminus. Two
iterations on OWL29.4 were required to reach convergence, at which point
a true set comprising 23 sequences was identified. Several partial matches
were also found, all of which are fragments and family members that fail
to make significant matches with one or more motifs.
 
An update on SPTR37_9f identified a true set of 32 sequences, and 4
partial matches.
Summary Information
  32 codes involving  4 elements
3 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
432323232
31233
21010
1234
True Positives
GREP_FRATU    GRP1_CHLTR    GRP2_CHLTR    GRPE_BACST    
GRPE_BACSU GRPE_BORBU GRPE_CAUCR GRPE_CLOAB
GRPE_DROME GRPE_ECOLI GRPE_HAEIN GRPE_HELPY
GRPE_LACLA GRPE_LEGPN GRPE_METMA GRPE_MYCTU
GRPE_STAAU GRPE_STRCO GRPE_SYNY3 GRPE_THETH
GRPE_YEAST GRPS_MYXXA O27350 O43047
O68444 O69267 O69297 O83245
O84400 O87776 P97576 Q18421
True Positive Partials
Codes involving 3 elements
GRPE_STRMU O66745 Q96515
Codes involving 2 elements
O51868
Sequence Titles
GREP_FRATU  GRPE PROTEIN - FRANCISELLA TULARENSIS.        
GRP1_CHLTR GRPE-LIKE PROTEIN - CHLAMYDIA TRACHOMATIS.
GRP2_CHLTR GRPE-LIKE PROTEIN - CHLAMYDIA TRACHOMATIS.
GRPE_BACST GRPE PROTEIN - BACILLUS STEAROTHERMOPHILUS.
GRPE_BACSU GRPE PROTEIN - BACILLUS SUBTILIS.
GRPE_BORBU GRPE PROTEIN - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
GRPE_CAUCR GRPE PROTEIN - CAULOBACTER CRESCENTUS.
GRPE_CLOAB GRPE PROTEIN - CLOSTRIDIUM ACETOBUTYLICUM.
GRPE_DROME GRPE PROTEIN HOMOLOG PRECURSOR - DROSOPHILA MELANOGASTER (FRUIT FLY).
GRPE_ECOLI HEAT SHOCK PROTEIN GRPE (HEAT SHOCK PROTEIN B25.3) (HSP24) - ESCHERICHIA COLI.
GRPE_HAEIN GRPE PROTEIN - HAEMOPHILUS INFLUENZAE.
GRPE_HELPY GRPE PROTEIN - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
GRPE_LACLA GRPE PROTEIN - LACTOCOCCUS LACTIS (SUBSP. LACTIS) (STREPTOCOCCUS LACTIS).
GRPE_LEGPN GRPE PROTEIN - LEGIONELLA PNEUMOPHILA.
GRPE_METMA GRPE PROTEIN HOMOLOG - METHANOSARCINA MAZEI.
GRPE_MYCTU GRPE PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
GRPE_STAAU GRPE PROTEIN (HSP20) - STAPHYLOCOCCUS AUREUS.
GRPE_STRCO GRPE PROTEIN - STREPTOMYCES COELICOLOR.
GRPE_SYNY3 GRPE PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
GRPE_THETH GRPE PROTEIN - THERMUS AQUATICUS (SUBSP. THERMOPHILUS).
GRPE_YEAST GRPE PROTEIN HOMOLOG PRECURSOR - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
GRPS_MYXXA GRPS PROTEIN - MYXOCOCCUS XANTHUS.
O27350 HEAT SHOCK PROTEIN GRPE - METHANOBACTERIUM THERMOAUTOTROPHICUM.
O43047 PUTATIVE MITOCHONDRIAL IMPORT PROTEIN - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O68444 GRPE - AGROBACTERIUM TUMEFACIENS.
O69267 HEAT SHOCK PROTEIN - BACILLUS SPHAERICUS.
O69297 HEAT SHOCK PROTEIN GRPE - CAMPYLOBACTER JEJUNI.
O83245 GRPE PROTEIN (GRPE) - TREPONEMA PALLIDUM.
O84400 HSP-70 COFACTOR - CHLAMYDIA TRACHOMATIS.
O87776 HEAT SHOCK PROTEIN GRPE - LACTOBACILLUS SAKE.
P97576 MT-GRPE#1 PRECURSOR - RATTUS NORVEGICUS (RAT).
Q18421 C34C12.8 PROTEIN - CAENORHABDITIS ELEGANS.

GRPE_STRMU GRPE PROTEIN - STREPTOCOCCUS MUTANS.
O66745 HEAT SHOCK PROTEIN GRPE - AQUIFEX AEOLICUS.
Q96515 AR192 - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).

O51868 GRPE PROTEIN - LEPTOSPIRA INTERROGANS.
Scan History
OWL29_4    2  720  NSINGLE    
SPTR37_9f 2 39 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LRLYAEFENYKRRIQKE GRPE_STAAU 78 78 -
LRVQADFENYKRRSRLE GRPE_BACSU 56 56 -
LRSRAEIENLRRRTEQD GRPE_HAEIN 102 102 -
LRKQAEFENFRKRLEKE GRPE_BORBU 53 53 -
LRLSAEYENYRNRTAKE GRPE_CLOAB 74 74 -
QRVQADFANYRKRALRD GRPE_MYCTU 63 63 -
LRVKAEMENLRRRTELD GRPE_ECOLI 63 63 -
LRVSAEMQNVQRRGNEE GRPE_LACLA 53 53 -
LRSVADFRNLQQVTKKD GRPE_YEAST 89 89 -

Motif 2 width=16
Element Seqn Id St Int Rpt
VLTDILPAIDNIERAL GRPE_STAAU 106 11 -
IVTDLLPALDSFERAL GRPE_BACSU 84 11 -
FSKDILNTIDNLERAL GRPE_HAEIN 130 11 -
IMKDVVNFLDNLERAI GRPE_BORBU 81 11 -
VINEMLPTLDNLERAA GRPE_CLOAB 102 11 -
VVSQLLGVLDDLERAR GRPE_MYCTU 91 11 -
FINELLPVIDSLDRAL GRPE_ECOLI 91 11 -
LAKKILSSLDNLERAL GRPE_LACLA 81 11 -
FAKDLLESVDNFGHAL GRPE_YEAST 117 11 -

Motif 3 width=16
Element Seqn Id St Int Rpt
GEAFDPNIHQAVVQDD GRPE_STAAU 160 38 -
GQEFDPNLHQAVMQAE GRPE_BACSU 138 38 -
GEAFNPDLHQAISMQP GRPE_HAEIN 186 40 -
GENFDPSRHEAISIEE GRPE_BORBU 136 39 -
EGKFDPNLHNAVMHIE GRPE_CLOAB 152 34 -
GEDFDPVLHEAVQHEG GRPE_MYCTU 139 32 -
NVPLDPNVHQAIAMVE GRPE_ECOLI 146 39 -
YESFDHNLHMAVQTVP GRPE_LACLA 130 33 -
GEPFDPNKHEATFELP GRPE_YEAST 176 43 -

Motif 4 width=20
Element Seqn Id St Int Rpt
ELQKGYKLKDRVLRPSMVKV GRPE_STAAU 187 11 -
EMQKGYKLKDRVIRPSMVKV GRPE_BACSU 165 11 -
VLQKGYTLNGRVIRPAMVMV GRPE_HAEIN 213 11 -
VYQKGYCYNDRILRTAKVKV GRPE_BORBU 163 11 -
VLQKGYKRGDKVLRHSMVKV GRPE_CLOAB 179 11 -
VMRQGYQLGEQVLRHALVGV GRPE_MYCTU 167 12 -
IMQKGYTLNGRTIRAAMVTV GRPE_ECOLI 173 11 -
VFQKGYQLHERLLRPAMVVV GRPE_LACLA 158 12 -
VQQLGFTLNDRVIRPAKVGI GRPE_YEAST 203 11 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
LRLYADFENFRRRTRQE GRPE_BACST 91 91 -
LRLRADFDNMRRRQQLD O69267 68 68 -
LRLYAEFENYKRRIQKE GRPE_STAAU 78 78 -
LRVQADFENYKRRSRLE GRPE_BACSU 56 56 -
LRSQAEIKNIQMRNQKE O87776 69 69 -
VRALAELENVRRRMERE GRPE_LEGPN 69 69 -
LRSRAEIENLRRRTEQD GRPE_HAEIN 102 102 -
LRAKAEMENIRKRAERD GREP_FRATU 64 64 -
LRLAAEMDNLRRRTERD O68444 52 52 -
MRANAEFENIKKRMEKE O69297 47 47 -
LRKQAEFENFRKRLEKE GRPE_BORBU 53 53 -
VRHAADLENYRKRALKE GRPS_MYXXA 84 84 -
LRLLADFDNYRKRMEEE GRPE_THETH 43 43 -
LRYAAEAENTKRRAERE GRPE_CAUCR 27 27 -
LRLSAEYENYRNRTAKE GRPE_CLOAB 74 74 -
QRSLAETENVRRRGIKQ Q18421 98 98 -
LRKAADLENYRKRALRE O83245 69 69 -
RQSLADYRNLENRMKRD O43047 82 82 -
LMALAEAENSRKRLQKE GRP2_CHLTR 45 45 -
LMALAEAENSRKRLQKE O84400 45 45 -
QRVQADFANYRKRALRD GRPE_MYCTU 63 63 -
LMALAEAENSRKRLQKE GRP1_CHLTR 45 45 -
QRLQAEYQNYRRRVERD GRPE_STRCO 64 64 -
KRALADTENLRQRSQKL P97576 79 79 -
LRVKAEMENLRRRTELD GRPE_ECOLI 63 63 -
LRVSAEMQNVQRRGNEE GRPE_LACLA 53 53 -
LRVHADFENVKKRLERD GRPE_HELPY 58 58 -
KRSLADSENMRNRLNKQ GRPE_DROME 77 77 -
VALAAEFDNFRKRTQRE GRPE_SYNY3 101 101 -
LRSVADFRNLQQVTKKD GRPE_YEAST 89 89 -
QRLQADFDNYKKQMEKQ O27350 48 48 -
FRLAADFDNFRKRTARQ GRPE_METMA 71 71 -

Motif 2 width=16
Element Seqn Id St Int Rpt
LVSDLLPALDNFERAL GRPE_BACST 119 11 -
LLSDLLPVLDNFERAL O69267 96 11 -
VLTDILPAIDNIERAL GRPE_STAAU 106 11 -
IVTDLLPALDSFERAL GRPE_BACSU 84 11 -
LAKDVLPVLDNLERAL O87776 97 11 -
LISALLPVVDSLEQAL GRPE_LEGPN 97 11 -
FSKDILNTIDNLERAL GRPE_HAEIN 130 11 -
FAKELLPVIDSIEQAL GREP_FRATU 92 11 -
FARDMLAVSDNLRRAL O68444 80 11 -
FAKDLLDVLDALEAAV O69297 75 11 -
IMKDVVNFLDNLERAI GRPE_BORBU 81 11 -
LLKDLLPVMDNLDRAI GRPS_MYXXA 112 11 -
ALRALLPVLDDLDRAL GRPE_THETH 71 11 -
FARDLLGAADNLGRAT GRPE_CAUCR 55 11 -
VINEMLPTLDNLERAA GRPE_CLOAB 102 11 -
FCKDLLEVSDILDIAV Q18421 126 11 -
LLADIVAVLDDFDRAI O83245 97 11 -
LTKDLLDSVDNLERAL O43047 110 11 -
ALMDFLPSIESMEKAL GRP2_CHLTR 73 11 -
ALMDFLPPIESMEKAL O84400 73 11 -
VVSQLLGVLDDLERAR GRPE_MYCTU 91 11 -
ALLDFLPPMESMEKAL GRP1_CHLTR 73 11 -
LLSELLPVLDDVGRAR GRPE_STRCO 92 11 -
FCKDLLEVADILEKAT P97576 107 11 -
FINELLPVIDSLDRAL GRPE_ECOLI 91 11 -
LAKKILSSLDNLERAL GRPE_LACLA 81 11 -
IALDLLPVIDALLGAH GRPE_HELPY 86 11 -
FCKDLLEVADTLGHAT GRPE_DROME 105 11 -
TITELLPVVDNFERAR GRPE_SYNY3 129 11 -
FAKDLLESVDNFGHAL GRPE_YEAST 117 11 -
LILKLLDVYEDLERAI O27350 76 11 -
VLLDFVEVTDNFDRAI GRPE_METMA 99 11 -

Motif 3 width=16
Element Seqn Id St Int Rpt
GKPFDPHLHQAVMQVE GRPE_BACST 173 38 -
GEQFDPNIHQAVMQEQ O69267 150 38 -
GEAFDPNIHQAVVQDD GRPE_STAAU 160 38 -
GQEFDPNLHQAVMQAE GRPE_BACSU 138 38 -
GQAFDPTIHQAVQTVA O87776 148 35 -
GQTFDPQQHEAMSMQP GRPE_LEGPN 150 37 -
GEAFNPDLHQAISMQP GRPE_HAEIN 186 40 -
GEKFDPNLHEAMAMIP GREP_FRATU 146 38 -
GQKFDPNFHQAMFEVP O68444 140 44 -
EKEFDPNLHEAMFHVD O69297 128 37 -
GENFDPSRHEAISIEE GRPE_BORBU 136 39 -
GQVFDPRVHEAIQQVE GRPS_MYXXA 166 38 -
GEAFDPRYHEAVGLLP GRPE_THETH 123 36 -
GDKFDPHLHQAVTEQP GRPE_CAUCR 113 42 -
EGKFDPNLHNAVMHIE GRPE_CLOAB 152 34 -
NEKFDPNLHEAVFQIP Q18421 186 44 -
GERFDPNLHEALSMSP O83245 157 44 -
GEDFDPNIHEAVFQIP O43047 172 46 -
GELFNPYLHEAVEIEE GRP2_CHLTR 128 39 -
GELFNPYLHEAVEIEE O84400 128 39 -
GEDFDPVLHEAVQHEG GRPE_MYCTU 139 32 -
GELFNPYLHEAVEIEE GRP1_CHLTR 128 39 -
GEPFDPTIHEALMHSY GRPE_STRCO 139 31 -
GAKFDPYEHEALFHTP P97576 167 44 -
NVPLDPNVHQAIAMVE GRPE_ECOLI 146 39 -
YESFDHNLHMAVQTVP GRPE_LACLA 130 33 -
LEEFDPHFHNAIMQVK GRPE_HELPY 139 37 -
NQKFDPNQHEALFQKE GRPE_DROME 164 43 -
GKPFDPKYHEAMLREP GRPE_SYNY3 184 39 -
GEPFDPNKHEATFELP GRPE_YEAST 176 43 -
GEKFDPFLHEAVMVED O27350 124 32 -
AGEFDPHRHEAIHHIE GRPE_METMA 154 39 -

Motif 4 width=20
Element Seqn Id St Int Rpt
EFQKGYKLKDRVIRPAMVKV GRPE_BACST 200 11 -
ELQKGYILKDRVLRPTMVSV O69267 177 11 -
ELQKGYKLKDRVLRPSMVKV GRPE_STAAU 187 11 -
EMQKGYKLKDRVIRPSMVKV GRPE_BACSU 165 11 -
VFQKGYYLKDRVLRPAMVVV O87776 176 12 -
VFQKGYKLSDRVIRPARVIV GRPE_LEGPN 177 11 -
VLQKGYTLNGRVIRPAMVMV GRPE_HAEIN 213 11 -
VFQKGYMLNGRIVRAAKVVI GREP_FRATU 173 11 -
VIQAGFTIGDRVLRPAMVGV O68444 167 11 -
VLQKGYKIADRVIRPTKVSV O69297 155 11 -
VYQKGYCYNDRILRTAKVKV GRPE_BORBU 163 11 -
EVVRGFYLNERLVRPAMVVV GRPS_MYXXA 193 11 -
VFQRGFRMGEALVRPARVAV GRPE_THETH 147 8 -
VMQAGYELMGRLVRPAMVAV GRPE_CAUCR 140 11 -
VLQKGYKRGDKVLRHSMVKV GRPE_CLOAB 179 11 -
CTKIGYSLKERPIRPAQVGV Q18421 215 13 -
ELQKGYRVRNRILRHAKVMV O83245 184 11 -
CESKGFQLNGRVIRPAKVGV O43047 199 11 -
EFTKGYKIGDRPIRVAKVKV GRP2_CHLTR 155 11 -
EFTKGYKIGDRPIRVAKVKV O84400 155 11 -
VMRQGYQLGEQVLRHALVGV GRPE_MYCTU 167 12 -
EFTKGYKIGDRPIRVAKVKV GRP1_CHLTR 155 11 -
ILQPGYRIGERTIRPARVAV GRPE_STRCO 166 11 -
VSKVGYKLHGRTLRPALVGV P97576 194 11 -
IMQKGYTLNGRTIRAAMVTV GRPE_ECOLI 173 11 -
VFQKGYQLHERLLRPAMVVV GRPE_LACLA 158 12 -
VLQQGYKYKGRVLRPAMVSI GRPE_HELPY 166 11 -
VTKLGYKLHERCIRPALVGV GRPE_DROME 191 11 -
ELVRGYLLDDIVLRHSMVKV GRPE_SYNY3 211 11 -
VQQLGFTLNDRVIRPAKVGI GRPE_YEAST 203 11 -
ELSRGYRLNDRIIKHSIVKV O27350 151 11 -
IYKEGYALNEKVVRPALVSV GRPE_METMA 181 11 -