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PR00715

Identifier
MAN6PRECEPTR  [View Relations]  [View Alignment]  
Accession
PR00715
No. of Motifs
9
Creation Date
12-JUN-1997  (UPDATE 07-JUN-1999)
Title
Cation-dependent mannose-6-phosphate receptor signature
Database References

INTERPRO; IPR000296
Literature References
1. LUDWIG, T., RUTHER, U., METZGER, R., COPELAND, N.G., JENKINS, N.A.,
LOBEL, P. AND HOFLACK, B.
Gene and pseudogene of the mouse cation-dependent mannose 6-phosphate
receptor. Genomic organization, expression, and chromosomal localization.
J.BIOL.CHEM. 267(17) 12211-12219 (1992).
 
2. DAHMS N.M., LOBEL P., BREITMEYER J., CHIRGWIN J.M., KORNFELD S.
46 kD mannose 6-phosphate receptor: cloning, expression, and homology
to the 215 kD mannose 6-phosphate receptor. 
CELL 50(2) 181-192 (1987).
 
3. DAHMS, N.M. AND KORNFELD, S.
The cation-dependent mannose 6-phosphate receptor. Structural requirements
for mannose 6-phosphate binding and oligomerization.
J.BIOL.CHEM. 264(19) 11458-11467 (1989).

Documentation
The cation dependent mannose-6-phosphate (man-6-P) receptor is one of two
transmembrane proteins involved in the transport of lysosomal enzymes from
the Golgi complex and the cell surface to lysosomes [1]. Lysosomal enzymes
bearing phosphomannosyl residues bind specifically to man-6-P receptors in
the Golgi apparatus and the resulting receptor-ligand complex is transported
to an acidic prelyosomal compartment, where the low pH mediates dissociation
of the complex. Binding is optimal in the presence of divalent cations.
 
The amino acid sequence is a single polypeptide chain that contains a
putative signal sequence and a transmembrane domain [2]. Studies have
indicated that the receptor is a transmembrane protein with an
extracytoplasmic N-terminal domain [2]. This domain contains all the
information necessary both for ligand binding and for acid-dependent
ligand dissociation [3].
 
MAN6PRECEPTR is a 9-element fingerprint that provides a signature for
mannose-6-phosphate receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved regions
spanning the full alignment length - motifs 1-7 span the putative lumenal
domain; motif 8 lies in a putative TM domain; and motif 9 lies in the
proposed cytoplasmic domain. A single iteration on OWL29.3 was required
to reach convergence, no further sequences being identified beyond the
starting set. Three partial matches were found, all of which are
mannose-6-phosphate receptor fragments.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9333333333
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
MPRD_BOVIN    MPRD_HUMAN    MPRD_MOUSE    
Sequence Titles
MPRD_BOVIN  CATION-DEPENDENT MANNOSE-6-PHOSPHATE RECEPTOR PRECURSOR (CD MAN-6-P RECEPTOR) (CD-MPR) (MPR 46) - BOS TAURUS (BOVINE). 
MPRD_HUMAN CATION-DEPENDENT MANNOSE-6-PHOSPHATE RECEPTOR PRECURSOR (CD MAN-6-P RECEPTOR) (CD-MPR) (MPR 46) - HOMO SAPIENS (HUMAN).
MPRD_MOUSE CATION-DEPENDENT MANNOSE-6-PHOSPHATE RECEPTOR PRECURSOR (CD MAN-6-P RECEPTOR) (CD-MPR) (MPR 46) - MUS MUSCULUS (MOUSE).
Scan History
OWL29_3    1  50   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
VAVRESWQTEEKTCDLVGEKGKES MPRD_HUMAN 19 19 -
VAVRESWQIEEKSCDLVGEKDKES MPRD_MOUSE 20 20 -
VAVRESWQTEEKTCDLVGEKGKES MPRD_BOVIN 21 21 -

Motif 2 width=20
Element Seqn Id St Int Rpt
PLFNKSFESTVGQGSDTYIY MPRD_HUMAN 54 11 -
PLFNKSFESTVGQGSDTYSY MPRD_MOUSE 55 11 -
PLFNKSFESTVGQSPDMYSY MPRD_BOVIN 56 11 -

Motif 3 width=21
Element Seqn Id St Int Rpt
FRVCREAGNHTSGAGLVQINK MPRD_HUMAN 75 1 -
FRVCREASNHSSGAGLVQINK MPRD_MOUSE 76 1 -
FRVCREAGNHSSGAGLVQINK MPRD_BOVIN 77 1 -

Motif 4 width=19
Element Seqn Id St Int Rpt
KETVVGRLNETHIFNGSNW MPRD_HUMAN 99 3 -
KETVVGRINETHIFNGSNW MPRD_MOUSE 100 3 -
KETVVGRFNETQIFNGSNW MPRD_BOVIN 101 3 -

Motif 5 width=20
Element Seqn Id St Int Rpt
IMLIYKGGDEYDNHCGKEQR MPRD_HUMAN 118 0 -
IMLIYKGGDEYDNHCGKEQR MPRD_MOUSE 119 0 -
IMLIYKGGDEYDNHCGREQR MPRD_BOVIN 120 0 -

Motif 6 width=20
Element Seqn Id St Int Rpt
VMISCNRHTLADNFNPVSEE MPRD_HUMAN 141 3 -
VMISCNRHTLAANFNPVSEE MPRD_MOUSE 142 3 -
VMISCNRHTLADNFNPVSEE MPRD_BOVIN 143 3 -

Motif 7 width=20
Element Seqn Id St Int Rpt
KVQDCFYLFEMDSSLACSPE MPRD_HUMAN 163 2 -
KVQDCFYLFEMDSSLACSPE MPRD_MOUSE 164 2 -
KVQDCFYLFEMDSSLACSPE MPRD_BOVIN 165 2 -

Motif 8 width=22
Element Seqn Id St Int Rpt
ILLVTFASLVAVYVVGGFLYQR MPRD_HUMAN 191 8 -
ILLVIFASLVAVYIIGGFLYQR MPRD_MOUSE 192 8 -
ILLVTLASLVAVYIIGGFLYQR MPRD_BOVIN 193 8 -

Motif 9 width=19
Element Seqn Id St Int Rpt
YRGVGDDQLGEESEERDDH MPRD_HUMAN 255 42 -
YRGVGDDQLGEESEERDDH MPRD_MOUSE 256 42 -
YRGVGDDQLGEESEERDDH MPRD_BOVIN 257 42 -
Final Motifs
Motif 1  width=24
Element Seqn Id St Int Rpt
VAVRESWQTEEKTCDLVGEKGKES MPRD_HUMAN 19 19 -
VAVRESWQIEEKSCDLVGEKDKES MPRD_MOUSE 20 20 -
VAVRESWQTEEKTCDLVGEKGKES MPRD_BOVIN 21 21 -

Motif 2 width=20
Element Seqn Id St Int Rpt
PLFNKSFESTVGQGSDTYIY MPRD_HUMAN 54 11 -
PLFNKSFESTVGQGSDTYSY MPRD_MOUSE 55 11 -
PLFNKSFESTVGQSPDMYSY MPRD_BOVIN 56 11 -

Motif 3 width=21
Element Seqn Id St Int Rpt
FRVCREAGNHTSGAGLVQINK MPRD_HUMAN 75 1 -
FRVCREASNHSSGAGLVQINK MPRD_MOUSE 76 1 -
FRVCREAGNHSSGAGLVQINK MPRD_BOVIN 77 1 -

Motif 4 width=19
Element Seqn Id St Int Rpt
KETVVGRLNETHIFNGSNW MPRD_HUMAN 99 3 -
KETVVGRINETHIFNGSNW MPRD_MOUSE 100 3 -
KETVVGRFNETQIFNGSNW MPRD_BOVIN 101 3 -

Motif 5 width=20
Element Seqn Id St Int Rpt
IMLIYKGGDEYDNHCGKEQR MPRD_HUMAN 118 0 -
IMLIYKGGDEYDNHCGKEQR MPRD_MOUSE 119 0 -
IMLIYKGGDEYDNHCGREQR MPRD_BOVIN 120 0 -

Motif 6 width=20
Element Seqn Id St Int Rpt
VMISCNRHTLADNFNPVSEE MPRD_HUMAN 141 3 -
VMISCNRHTLAANFNPVSEE MPRD_MOUSE 142 3 -
VMISCNRHTLADNFNPVSEE MPRD_BOVIN 143 3 -

Motif 7 width=20
Element Seqn Id St Int Rpt
KVQDCFYLFEMDSSLACSPE MPRD_HUMAN 163 2 -
KVQDCFYLFEMDSSLACSPE MPRD_MOUSE 164 2 -
KVQDCFYLFEMDSSLACSPE MPRD_BOVIN 165 2 -

Motif 8 width=22
Element Seqn Id St Int Rpt
ILLVTFASLVAVYVVGGFLYQR MPRD_HUMAN 191 8 -
ILLVIFASLVAVYIIGGFLYQR MPRD_MOUSE 192 8 -
ILLVTLASLVAVYIIGGFLYQR MPRD_BOVIN 193 8 -

Motif 9 width=19
Element Seqn Id St Int Rpt
YRGVGDDQLGEESEERDDH MPRD_HUMAN 255 42 -
YRGVGDDQLGEESEERDDH MPRD_MOUSE 256 42 -
YRGVGDDQLGEESEERDDH MPRD_BOVIN 257 42 -