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PR00679

Identifier
PROHIBITIN  [View Relations]  [View Alignment]  
Accession
PR00679
No. of Motifs
7
Creation Date
05-MAR-1997  (UPDATE 23-JUN-1999)
Title
Prohibitin signature
Database References

PFAM; PF01145 Band_7
INTERPRO; IPR000163
Literature References
1. NUELL, M.J., STEWART, D.A., WALKER, L., FRIEDMAN, V., WOOD, C.M.,
OWENS, G.A., SMITH, J.R., SCHNEIDER, E.L., DELL' ORCO R., LUMPKIN C.K.,
DANNER, D.B. AND MCCLUNG, J.K.
Prohibitin, an evolutionarily conserved intracellular protein that
blocks DNA synthesis in normal fibroblasts and hela cells.
MOL.CELL.BIOL. 11(3) 1372-1381 (1991).
 
2. SATO T., SAITO H., SWENSEN J., OLIFANT A., WOOD C., DANNER D.,    
SAKAMOTO T., TAKITA K., KASUMI F. AND MIKI, Y.
The human prohibitin gene located on chromosome 17q21 is mutated in
sporadic breast cancer. 
CANCER RES. 52(6) 1643-1646 (1992).

Documentation
Genes that negatively regulate proliferation inside the cell are of
considerable interest because of the implications in processes such as
development and cancer [1]. Prohibitin, a novel cytoplasmic anti-
proliferative protein widely expressed in a variety of tissues, inhibits
DNA synthesis. Studies have suggested that prohibitin may be a suppressor
gene and is associated with tumor development and/or progression of at
least some breast cancers [2]. Sequence comparisons suggest that the
prohibitin gene is an analogue of Cc, a Drosophila gene that is vital for
normal development [1].
 
PROHIBITIN is a 7-element fingerprint that provides a signature for the
prohibitins. The fingerprint was derived from an initial alignment of
7 sequences: the motifs were drawn from the central portion of the
alignment - motif 2 contains Val and Arg residues known to be mutated in
sporadic breast cancer. A single iteration on OWL29.1 was required to reach 
convergence, no further sequences being identified beyond the starting set.
Two partial matches were found: L2CC_DROME is the Drosophila Cc analogue,
which matches motifs 1-3 and 6 and 7; and D9090073 is a C-terminal fragment
that matches motifs 6 and 7.
 
An update on SPTR37_9f identified a true set of 13 sequences, and 3
partial matches.
Summary Information
  13 codes involving  7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
713131313131313
61110111
50000000
40000000
30000000
20000011
1234567
True Positives
O01720        O04331        O04361        O35129        
O49460 O61074 PHB_HUMAN PHB_MOUSE
PHB_YEAST Q61336 Q99623 YG4W_YEAST
YWB1_CAEEL
True Positive Partials
Codes involving 6 elements
L2CC_DROME
Codes involving 2 elements
P72754
Sequence Titles
O01720      PROHIBITIN-LIKE MOLECULE TC-PRO-1 - TOXOCARA CANIS. 
O04331 PROHIBITIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O04361 PROHIBITIN - NICOTIANA TABACUM (COMMON TOBACCO).
O35129 THE SPLICE JUNCTIONS AT POSITIONS 91653 AND 91982 FOLLOW THE NONCANONICAL AT-AC RULE - MUS MUSCULUS (MOUSE).
O49460 PROHIBITIN-LIKE PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O61074 PROHIBITIN - TRYPANOSOMA BRUCEI RHODESIENSE.
PHB_HUMAN PROHIBITIN - HOMO SAPIENS (HUMAN).
PHB_MOUSE PROHIBITIN (B-CELL RECEPTOR ASSOCIATED PROTEIN 32) (BAP 32) - MUS MUSCULUS (MOUSE), AND RATTUS NORVEGICUS (RAT).
PHB_YEAST PROHIBITIN - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Q61336 BCR-ASSOCIATED PROTEIN 37 (IGM B-CELL RECEPTOR ASSOCIATED PROTEIN 37) (BAP) - MUS MUSCULUS (MOUSE).
Q99623 B-CELL RECEPTOR ASSOCIATED PROTEIN - HOMO SAPIENS (HUMAN).
YG4W_YEAST HYPOTHETICAL 34.9 KD PROTEIN IN SMI1-PHO81 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
YWB1_CAEEL HYPOTHETICAL 31.8 KD PROTEIN IN CHROMOSOME II - CAENORHABDITIS ELEGANS.

L2CC_DROME L(2)37CC PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY).

P72754 PROHIBITIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
Scan History
OWL29_1    1  50   NSINGLE    
SPTR37_9f 2 75 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
RTKPKSIATNTGTKDLQ PHB_YEAST 72 72 -
RAKPRNVASLTGTKDLQ YG4W_YEAST 101 101 -
RSRPRNVPVITGSKDLQ PHB_HUMAN 70 70 -
RSRPRNVPVITGSKDLQ PHB_RAT 70 70 -
RARPRKISSPTGSKDLQ S46996 83 83 -
RTKPKSIATNTGTKDLQ SCU16737 72 72 -
RARPNQIRSPTGSKDLQ YWB1_CAEEL 75 75 -

Motif 2 width=20
Element Seqn Id St Int Rpt
VNITCRVLSRPDVVQLPTIY YG4W_YEAST 119 1 -
VNITLRILFRPVASQLPRIF PHB_HUMAN 88 1 -
VSLTLRVLHRPEVLQLPAIY PHB_YEAST 90 1 -
VNITLRILFRPVASQLPRIY PHB_RAT 88 1 -
VNISLRVLSRPNAQELPSMY S46996 101 1 -
VSLTLRVLHRPEVLQLPAIY SCU16737 90 1 -
VNIGLRVLSRPNPEHLVHIY YWB1_CAEEL 93 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GEDYDERVLPSITTEILKS PHB_RAT 111 3 -
GLDYEERVLPSIVNEVLKS S46996 124 3 -
GLDYDERVLPSIGNEVLKS SCU16737 113 3 -
GEDYDERVLPSITTEILKS PHB_HUMAN 111 3 -
GQDYDERVLPSIVNEVLKA YG4W_YEAST 142 3 -
GQNWEERVLPSICNEVLKG YWB1_CAEEL 116 3 -
GLDYDERVLPSIGNEVLKS PHB_YEAST 113 3 -

Motif 4 width=17
Element Seqn Id St Int Rpt
FNASQLITQREKVSRLI YG4W_YEAST 165 4 -
FDAAELITQREIISQKI PHB_YEAST 136 4 -
FDAAELITQREIISQKI SCU16737 136 4 -
FNASQLITQRAQVSLLI S46996 147 4 -
FDAGELITQRELVSRQV PHB_RAT 134 4 -
FDAGELITQRELVSRQV PHB_HUMAN 134 4 -
FNASQLITQRQQVSMLV YWB1_CAEEL 139 4 -

Motif 5 width=20
Element Seqn Id St Int Rpt
RAATFGLILDDVSLTHLTFG PHB_HUMAN 157 6 -
RANEFGIKLEDVSITHMTFG PHB_YEAST 159 6 -
RANEFGIKLEDVSITHMTFG SCU16737 159 6 -
RAKDFSLILDDVAITELSFS S46996 170 6 -
RAATFGLILDDVSLTHLTFG PHB_RAT 157 6 -
RALDFNIILDDVSLTELAFS YWB1_CAEEL 162 6 -
RASKFNILLDDVSITYMTFS YG4W_YEAST 188 6 -

Motif 6 width=24
Element Seqn Id St Int Rpt
AVEAKQVAQQEAQRAQFLVEKAKQ S46996 195 5 -
AVEAKQVAQQEAERARFVVEKAEQ PHB_RAT 182 5 -
AVEAKQVAQQEAERARFVVEKAEQ PHB_HUMAN 182 5 -
AVEAKQIAQQDAQRAAFVVDKARQ YG4W_YEAST 213 5 -
AVEAKQVAAQEAQRATFYVERAKQ YWB1_CAEEL 187 5 -
AVEQKQIAQQDAERAKFLVEKAEQ PHB_YEAST 184 5 -
AVEQKQIAQQDAERAKFLVEKAEQ SCU16737 184 5 -

Motif 7 width=17
Element Seqn Id St Int Rpt
QERQASVIRAEGEAEGA SCU16737 207 -1 -
QQKKAAIISAEGDSKAA PHB_RAT 205 -1 -
QERQASVIRAEGEAESA PHB_YEAST 207 -1 -
QQKQEKIVQAEGEAESA YWB1_CAEEL 210 -1 -
QEKQGMVVRAQGEAKSA YG4W_YEAST 236 -1 -
QQKKAAIISAEGDSKAA PHB_HUMAN 205 -1 -
QEQRQKIVQAEGEAEAA S46996 218 -1 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
RARPRKISSPTGSKDLQ O35129 84 84 -
RARPRKISSPTGSKDLQ Q61336 83 83 -
RARPRKISSPTGSKDLQ Q99623 84 84 -
RSRPRNVPVITGSKDLQ PHB_MOUSE 70 70 -
RSRPRNVPVITGSKDLQ PHB_HUMAN 70 70 -
RTRPHTFSSVSGTKDLQ O04361 75 75 -
RAKPRNVASLTGTKDLQ YG4W_YEAST 101 101 -
RTKPHTFSSISGTKDLQ O04331 75 75 -
RARPNQIRSPTGSKDLQ YWB1_CAEEL 75 75 -
RTKPKSIATNTGTKDLQ PHB_YEAST 72 72 -
RARPYLVESTSGSRDLQ O49460 79 79 -
RSTPRAISTITGSKDLQ O01720 72 72 -
RIRPRVLKTMTGTKDLQ O61074 67 67 -

Motif 2 width=20
Element Seqn Id St Int Rpt
VNISLRVLSRPNAQELPSMY O35129 102 1 -
VNISLRVLSRPNAQELPSMY Q61336 101 1 -
VNISLRVLSRPNAQELPSMY Q99623 102 1 -
VNITLRILFRPVASQLPRIY PHB_MOUSE 88 1 -
VNITLRILFRPVASQLPRIF PHB_HUMAN 88 1 -
VHLTLRVLSRPEVARLPAIF O04361 93 1 -
VNITCRVLSRPDVVQLPTIY YG4W_YEAST 119 1 -
VNLTLRVLSRPEVSRLPYIF O04331 93 1 -
VNIGLRVLSRPNPEHLVHIY YWB1_CAEEL 93 1 -
VSLTLRVLHRPEVLQLPAIY PHB_YEAST 90 1 -
VKIGLRVLTRPMADQLPEIY O49460 97 1 -
VSITLRILHRPEPSKLPNIY O01720 90 1 -
VNISLRVLFRPQTDRLPQIY O61074 85 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GLDYEERVLPSIVNEVLKS O35129 125 3 -
GLDYEERVLPSIVNEVLKS Q61336 124 3 -
GLDYEERVLPSIVNEVLKS Q99623 125 3 -
GEDYDERVLPSITTEILKS PHB_MOUSE 111 3 -
GEDYDERVLPSITTEILKS PHB_HUMAN 111 3 -
GLEYDEKVLPSIGNEVLKA O04361 116 3 -
GQDYDERVLPSIVNEVLKA YG4W_YEAST 142 3 -
GLEYDEKVLPSIGNEVLKA O04331 116 3 -
GQNWEERVLPSICNEVLKG YWB1_CAEEL 116 3 -
GLDYDERVLPSIGNEVLKS PHB_YEAST 113 3 -
GENYSERVLPSIINETLKA O49460 120 3 -
GQDYAERVLPSITNEVLKA O01720 113 3 -
GMDYDERILPSISNEILKA O61074 108 3 -

Motif 4 width=17
Element Seqn Id St Int Rpt
FNASQLITQRAQVSLLI O35129 148 4 -
FNASQLITQRAQVSLLI Q61336 147 4 -
FNASQLITQRAQVSLLI Q99623 148 4 -
FDAGELITQRELVSRQV PHB_MOUSE 134 4 -
FDAGELITQRELVSRQV PHB_HUMAN 134 4 -
FNADQLLTERPQVSALV O04361 139 4 -
FNASQLITQREKVSRLI YG4W_YEAST 165 4 -
FNADQLLTERPHVSALV O04331 139 4 -
FNASQLITQRQQVSMLV YWB1_CAEEL 139 4 -
FDAAELITQREIISQKI PHB_YEAST 136 4 -
YNASQLITQREAVSREI O49460 143 4 -
FDAHEMITQRESVSHRV O01720 136 4 -
YKAEELIQKRDVVSARI O61074 131 4 -

Motif 5 width=20
Element Seqn Id St Int Rpt
RAKDFSLILDDVAITELSFS O35129 171 6 -
RAKDFSLILDDVAITELSFS Q61336 170 6 -
RAKDFSLILDDVAITELSFS Q99623 171 6 -
RAATFGLILDDVSLTHLTFG PHB_MOUSE 157 6 -
RAATFGLILDDVSLTHLTFG PHB_HUMAN 157 6 -
RAKDFNIVLDDVAITHLSYG O04361 162 6 -
RASKFNILLDDVSITYMTFS YG4W_YEAST 188 6 -
RAKDFNIVLDDVAITHLSYG O04331 162 6 -
RALDFNIILDDVSLTELAFS YWB1_CAEEL 162 6 -
RANEFGIKLEDVSITHMTFG PHB_YEAST 159 6 -
RAANFNVALDDVSITNLTFG O49460 166 6 -
RARQFGILLDDIAITHLSFG O01720 159 6 -
KVSQFGLVLEDLSLVDIQFG O61074 154 6 -

Motif 6 width=24
Element Seqn Id St Int Rpt
AVEAKQVAQQEAQRAQFLVEKAKQ O35129 196 5 -
AVEAKQVAQQEAQRAQFLVEKAKQ Q61336 195 5 -
AVEAKQVAQQEAQRAQFLVEKAKQ Q99623 196 5 -
AVEAKQVAQQEAERARFVVEKAEQ PHB_MOUSE 182 5 -
AVEAKQVAQQEAERARFVVEKAEQ PHB_HUMAN 182 5 -
AVEQKQVAQQEAERSKFVVMKAEQ O04361 187 5 -
AVEAKQIAQQDAQRAAFVVDKARQ YG4W_YEAST 213 5 -
AVEQKQVAQQEAERSKFVVMKADQ O04331 187 5 -
AVEAKQVAAQEAQRATFYVERAKQ YWB1_CAEEL 187 5 -
AVEQKQIAQQDAERAKFLVEKAEQ PHB_YEAST 184 5 -
AIEAKQVAAQEAERAKFIVEKAEQ O49460 191 5 -
AVEMKQVAQQEAEKARYLVETAEQ O01720 184 5 -
AVEQKQVAQQEAERFRYVVLENEQ O61074 179 5 -

Motif 7 width=17
Element Seqn Id St Int Rpt
QEQRQKIVQAEGEAEAA O35129 219 -1 -
QEQRQKIVQAEGEAEAA Q61336 218 -1 -
QEQRQKIVQAEGEAEAA Q99623 219 -1 -
QQKKAAIISAEGDSKAA PHB_MOUSE 205 -1 -
QQKKAAIISAEGDSKAA PHB_HUMAN 205 -1 -
QERRAAIIRAEGESESA O04361 210 -1 -
QEKQGMVVRAQGEAKSA YG4W_YEAST 236 -1 -
QERRAAVIRAEGESEAA O04331 210 -1 -
QQKQEKIVQAEGEAESA YWB1_CAEEL 210 -1 -
QERQASVIRAEGEAESA PHB_YEAST 207 -1 -
QDKRSAVIRAQGEAKSA O49460 214 -1 -
QMKIAAITTAEGDAQAA O01720 207 -1 -
QKRRAAVVRAEGEAESA O61074 202 -1 -