1. NUELL, M.J., STEWART, D.A., WALKER, L., FRIEDMAN, V., WOOD, C.M.,
OWENS, G.A., SMITH, J.R., SCHNEIDER, E.L., DELL' ORCO R., LUMPKIN C.K.,
DANNER, D.B. AND MCCLUNG, J.K.
Prohibitin, an evolutionarily conserved intracellular protein that
blocks DNA synthesis in normal fibroblasts and hela cells.
MOL.CELL.BIOL. 11(3) 1372-1381 (1991).
2. SATO T., SAITO H., SWENSEN J., OLIFANT A., WOOD C., DANNER D.,
SAKAMOTO T., TAKITA K., KASUMI F. AND MIKI, Y.
The human prohibitin gene located on chromosome 17q21 is mutated in
sporadic breast cancer.
CANCER RES. 52(6) 1643-1646 (1992).
Genes that negatively regulate proliferation inside the cell are of
considerable interest because of the implications in processes such as
development and cancer . Prohibitin, a novel cytoplasmic anti-
proliferative protein widely expressed in a variety of tissues, inhibits
DNA synthesis. Studies have suggested that prohibitin may be a suppressor
gene and is associated with tumor development and/or progression of at
least some breast cancers . Sequence comparisons suggest that the
prohibitin gene is an analogue of Cc, a Drosophila gene that is vital for
normal development .
PROHIBITIN is a 7-element fingerprint that provides a signature for the
prohibitins. The fingerprint was derived from an initial alignment of
7 sequences: the motifs were drawn from the central portion of the
alignment - motif 2 contains Val and Arg residues known to be mutated in
sporadic breast cancer. A single iteration on OWL29.1 was required to reach
convergence, no further sequences being identified beyond the starting set.
Two partial matches were found: L2CC_DROME is the Drosophila Cc analogue,
which matches motifs 1-3 and 6 and 7; and D9090073 is a C-terminal fragment
that matches motifs 6 and 7.
An update on SPTR37_9f identified a true set of 13 sequences, and 3