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PR00599

Identifier
MAPEPTIDASE  [View Relations]  [View Alignment]  
Accession
PR00599
No. of Motifs
4
Creation Date
08-SEP-1996  (UPDATE 10-JUN-1999)
Title
Methionine aminopeptidase-1 signature
Database References

PROSITE; PS00680 MAP_1; PS01202 MAP_2
INTERPRO; IPR001714
Literature References
1. ARFIN, S.M., KENDALL, R.L., HALL, L., WEAVER, L.H., STEWART, A.E.,
MATTHEWS, B.W. AND BRADSHAW, R.A.
Eukaryotic methionyl aminopeptidases: 2 classes of cobalt-dependent enzymes.
PROC.NATL.ACAD.SCI.U.S.A. 92 7714-7718 (1995).
 
2. KEELING, P.J. AND DOOLITTLE, W.F.
Methionine aminopeptidase-1: the map of the mitochondrion.
TRENDS BIOCHEM.SCI. 21(8) 285-286 (1996).
 
3. RODERICK, S.L. AND MATHEWS, B.W.
Structure of the cobalt-dependent methionine aminopeptidase from
Escherichia coli: a new type of proteolytic-enzyme.
BIOCHEMISTRY 32 3907-3912 (1993).

Documentation
Methionine aminopeptidase (MAP) removes the initiator, N-terminal,
methionine from nascent eukaryotic cytosolic and cytoplasmic prokaryotic
proteins where the penultimate amino acid is small and uncharged. MAP
enzymes are monomeric proteins that require cobalt ions for activity. Two
related families of MAPs have been described [1,2], which share sequence
similarity in the region of the cobalt-binding site [3]. The first of these
includes enzymes from prokaryotes as well as eukaryotic MAP-1. 
 
MAPEPTIDASE is a 4-element fingerprint that provides a signature for
methionine aminopeptidase 1. The fingerprint was derived from an initial
alignment of 8 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, motif 3 including part of the
region encoded by PROSITE pattern MAP_1 (PS00680), which contains residues
known to be involved in colbalt-binding. Two iterations on OWL28.1 were
required to reach convergence, at which point a true set comprising 15
sequences was identified. Several partial matches were also found,
including a number of fragments and X-PRO aminopeptidases.
 
An update on SPTR37_9f identified a true set of 33 sequences, and 19
partial matches.
Summary Information
  33 codes involving  4 elements
2 codes involving 3 elements
17 codes involving 2 elements
Composite Feature Index
433333333
32211
281619
1234
True Positives
AMP1_SYNY3    AMP1_YEAST    AMP2_HUMAN    AMP2_MOUSE    
AMP2_RAT AMP2_SYNY3 AMP2_YEAST AMP3_SYNY3
AMPM_ARCFU AMPM_BACSU AMPM_BORBU AMPM_ECOLI
AMPM_HAEIN AMPM_HELPY AMPM_MYCGE AMPM_MYCPN
AMPM_PYRFU AMPM_SALTY AMPM_SULSO AMPM_TREPA
O33008 O33343 O52353 O53797
O54208 O58362 O59730 O60085
O61540 O66489 O69937 O80587
O84859
True Positive Partials
Codes involving 3 elements
AMPM_METTH O34484
Codes involving 2 elements
AMPM_METJA AMPP_ECOLI O22523 O23182
O31689 O43846 O52579 O53048
O58691 O58885 O73806 O88076
P81535 PLS1_MOUSE Q96327 Y806_METJA
YQHT_BACSU
Sequence Titles
AMP1_SYNY3  PUTATIVE METHIONINE AMINOPEPTIDASE A (EC 3.4.11.18) (MAP) (PEPTIDASE M) - SYNECHOCYSTIS SP. (STRAIN PCC 6803). 
AMP1_YEAST METHIONINE AMINOPEPTIDASE 1 PRECURSOR (EC 3.4.11.18) (METAP 1) (PEPTIDASE M 1) (MAP) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
AMP2_HUMAN METHIONINE AMINOPEPTIDASE 2 (EC 3.4.11.18) (METAP 2) (PEPTIDASE M 2) (INITIATION FACTOR 2 ASSOCIATED 67 KD GLYCOPROTEIN) (P67) - HOMO SAPIENS (HUMAN).
AMP2_MOUSE METHIONINE AMINOPEPTIDASE 2 (EC 3.4.11.18) (METAP 2) (PEPTIDASE M 2) (INITIATION FACTOR 2 ASSOCIATED 67 KD GLYCOPROTEIN) (P67) - MUS MUSCULUS (MOUSE).
AMP2_RAT METHIONINE AMINOPEPTIDASE 2 (EC 3.4.11.18) (METAP 2) (PEPTIDASE M 2) (INITIATION FACTOR 2 ASSOCIATED 67 KD GLYCOPROTEIN) (P67) - RATTUS NORVEGICUS (RAT).
AMP2_SYNY3 PUTATIVE METHIONINE AMINOPEPTIDASE B (EC 3.4.11.18) (MAP) (PEPTIDASE M) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
AMP2_YEAST METHIONINE AMINOPEPTIDASE 2 (EC 3.4.11.18) (METAP 2) (PEPTIDASE M 2) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
AMP3_SYNY3 PUTATIVE METHIONINE AMINOPEPTIDASE C (EC 3.4.11.18) (MAP) (PEPTIDASE M) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
AMPM_ARCFU METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - ARCHAEOGLOBUS FULGIDUS.
AMPM_BACSU METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - BACILLUS SUBTILIS.
AMPM_BORBU METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
AMPM_ECOLI METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - ESCHERICHIA COLI.
AMPM_HAEIN METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - HAEMOPHILUS INFLUENZAE.
AMPM_HELPY METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
AMPM_MYCGE METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - MYCOPLASMA GENITALIUM.
AMPM_MYCPN METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - MYCOPLASMA PNEUMONIAE.
AMPM_PYRFU METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - PYROCOCCUS FURIOSUS.
AMPM_SALTY METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - SALMONELLA TYPHIMURIUM.
AMPM_SULSO METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - SULFOLOBUS SOLFATARICUS.
AMPM_TREPA METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - TREPONEMA PALLIDUM.
O33008 METHIONINE AMINOPEPTIDASE - MYCOBACTERIUM LEPRAE.
O33343 METHIONINE AMINOPEPTIDASE - MYCOBACTERIUM TUBERCULOSIS.
O52353 METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) - MYCOPLASMA GALLISEPTICUM.
O53797 METHIONINE AMINOPEPTIDASE - MYCOBACTERIUM TUBERCULOSIS, AND MYCOBACTERIUM BOVIS.
O54208 METHIONINE AMINOPEPTIDASE - STREPTOMYCES COELICOLOR.
O58362 295AA LONG HYPOTHETICAL METHIONYL AMINOPEPTIDASE - PYROCOCCUS HORIKOSHII.
O59730 PUTATIVE METHIONINE AMINOPEPTIDASE 1 - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O60085 METHIONINE AMINOPEPTIDASE - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O61540 METHIONINE AMINOPEPTIDASE - DROSOPHILA MELANOGASTER (FRUIT FLY).
O66489 METHIONYL AMINOPEPTIDASE - AQUIFEX AEOLICUS.
O69937 METHIONINE AMINOPEPTIDASE - STREPTOMYCES COELICOLOR.
O80587 PUTATIVE TRANSLATION INITIATION FACTOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O84859 METHIONINE AMINOPEPTIDASE - CHLAMYDIA TRACHOMATIS.

AMPM_METTH METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - METHANOBACTERIUM THERMOAUTOTROPHICUM.
O34484 YFLG PROTEIN - BACILLUS SUBTILIS.

AMPM_METJA METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) (MAP) (PEPTIDASE M) - METHANOCOCCUS JANNASCHII.
AMPP_ECOLI XAA-PRO AMINOPEPTIDASE (EC 3.4.11.9) (X-PRO AMINOPEPTIDASE) (AMINOPEPTIDASE P II) (APP-II) (AMINOACYLPROLINE AMINOPEPTIDASE) - ESCHERICHIA COLI.
O22523 DNA-BINDING PROTEIN GBP16 - ORYZA SATIVA (RICE).
O23182 HYPOTHETICAL 57.6 KD PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O31689 YKVY PROTEIN - BACILLUS SUBTILIS.
O43846 CELL CYCLE PROTEIN P38-2G4 HOMOLOG - HOMO SAPIENS (HUMAN).
O52579 AMINOPEPTIDASE - SHIGELLA FLEXNERI.
O53048 PROLIDASE-RELATED PROTEIN - LACTOBACILLUS DELBRUECKII.
O58691 356AA LONG HYPOTHETICAL DIPEPTIDASE - PYROCOCCUS HORIKOSHII.
O58885 351AA LONG HYPOTHETICAL X-PRO DIPEPTIDASE - PYROCOCCUS HORIKOSHII.
O73806 PAS1 - FUGU RUBRIPES (JAPANESE PUFFERFISH) (TAKIFUGU RUBRIPES).
O88076 METHIONINE AMINOPEPTIDASE A - ENTEROCOCCUS FAECALIS (STREPTOCOCCUS FAECALIS).
P81535 XAA-PRO DIPEPTIDASE (EC 3.4.13.9) (X-PRO DIPEPTIDASE) (PROLINE DIPEPTIDASE) (PROLIDASE) (IMIDODIPEPTIDASE) - PYROCOCCUS FURIOSUS.
PLS1_MOUSE PROLIFERATION-ASSOCIATED PROTEIN 1 (PROTEIN P38-2G4) - MUS MUSCULUS (MOUSE).
Q96327 PUTATIVE NUCLEAR DNA-BINDING PROTEIN G2P (ATG2) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Y806_METJA HYPOTHETICAL PEPTIDASE MJ0806 (EC 3.4.-.-) - METHANOCOCCUS JANNASCHII.
YQHT_BACSU PUTATIVE PEPTIDASE IN GCVT-SPOIIIAA INTERGENIC REGION (EC 3.4.-.-) - BACILLUS SUBTILIS.
Scan History
OWL28_1    2  100  NSINGLE    
SPTR37_9f 2 65 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
LCTSVNEVICHGVP AMPM_YEAST 192 192 -
TGCSLNNCAAHYTP I2A6_RAT 221 221 -
TGLSLNHCAAHFTP YBJ1_YEAST 92 92 -
TCISINEVVCHGIP AMPM_HAEIN 69 69 -
VCISINEVVCHGIP AMPM_ECOLI 69 69 -
ICVSVNEELVHGIP AMPM_BACSU 67 67 -
VCISINEVVCHGIP AMPM_SALTY 68 68 -
NCLSLNQTVIHGVA AMPM_MYCGE 67 67 -

Motif 2 width=17
Element Seqn Id St Int Rpt
DIVNIDVTVIKDGYFGD AMPM_HAEIN 92 9 -
DVMKVDYGVQVNGNIID YBJ1_YEAST 117 11 -
DICKIDFGTHISGRIID I2A6_RAT 246 11 -
DIVNLDVSLYYQGYHAD AMPM_YEAST 214 8 -
DKLTLDIGIDYHGYLCD AMPM_MYCGE 89 8 -
DIVNIDVTVIKDEFHGD AMPM_SALTY 91 9 -
DIISIDIGAKLNGYHGD AMPM_BACSU 89 8 -
DIVNIDVTVIKDGFHGD AMPM_ECOLI 92 9 -

Motif 3 width=13
Element Seqn Id St Int Rpt
VKPIRNLNGHSIG I2A6_RAT 322 59 -
FSVVREYCGHGVG AMPM_HAEIN 163 54 -
FSVVREYCGHGIG AMPM_ECOLI 162 53 -
FSVVREYVGHGVG AMPM_BACSU 159 53 -
FSVVREYCGHGIG AMPM_SALTY 161 53 -
YFLVKEFGGHGCG AMPM_MYCGE 160 54 -
CSVVRTYCGHGVG AMPM_YEAST 285 54 -
VKPCRNLCGHSIA YBJ1_YEAST 193 59 -

Motif 4 width=13
Element Seqn Id St Int Rpt
TKMEEGEHFAIET YBJ1_YEAST 224 18 -
VRLQEGMVICIEP AMPM_MYCGE 191 18 -
VVLQPGMTFTIEP AMPM_SALTY 192 18 -
PRLKPGMVLAIEP AMPM_BACSU 190 18 -
VVLKPGMTFTIEP AMPM_ECOLI 193 18 -
VILKPGMVFTIEP AMPM_HAEIN 194 18 -
TRMEEGEVYAIET I2A6_RAT 353 18 -
GVMKPGMVFTIEP AMPM_YEAST 316 18 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
TCISINEVVCHGIP AMPM_HAEIN 69 69 -
VCISINEVVCHGIP AMPM_ECOLI 69 69 -
ICVSVNEELVHGIP AMPM_BACSU 67 67 -
VCISINEVVCHGIP AMPM_SALTY 68 68 -
ICASVNEEVIHGIP AMPM_BORBU 69 69 -
ICTSINEVICHGIP AMP1_SYNY3 70 70 -
ICTSINEVVCHGIP AMP2_SYNY3 92 92 -
ICTSVNDRVIHGIP O33008 78 78 -
CCTSLNEVICHGIP O33343 104 104 -
ICTSVNEVVVHGIP O54208 67 67 -
ICASINDRVVHGIP O53797 78 78 -
ICTSLNEVICHGIP O84859 108 108 -
LCISINDEVVHGLP O66489 74 74 -
ACISINEEVIHGLP AMPM_TREPA 66 66 -
LCTSVNEVICHGVP AMP1_YEAST 192 192 -
ICASVNDAIVHGIP O69937 73 73 -
VCTSVNEIICHGIP O59730 182 182 -
VCMSLNEVVIHGIP AMPM_HELPY 68 68 -
TGCSLNNCAAHYTP AMP2_HUMAN 221 221 -
TGCSLNNCAAHYTP AMP2_MOUSE 221 221 -
TGCSLNNCAAHYTP AMP2_RAT 221 221 -
ICACVNNEVVHGIP AMP3_SYNY3 119 119 -
ANISINSDAAHFTP AMPM_ARCFU 55 55 -
VNLSINEIAAHYTP AMPM_PYRFU 52 52 -
NCLSLNETVIHGIA AMPM_MYCPN 67 67 -
ANLSLNEVAAHYTP O58362 52 52 -
TGCSLNNVAAHWTP O80587 174 174 -
NCLSLNQTVIHGVA AMPM_MYCGE 67 67 -
TGLSLNHCAAHFTP AMP2_YEAST 164 164 -
TGCSLNHCAAHYTP O61540 191 191 -
TGVSLNHCAAHYTP O60085 169 169 -
HCLSLNKTIIHGLA O52353 67 67 -
CNISINSEAAHYSP AMPM_SULSO 55 55 -

Motif 2 width=17
Element Seqn Id St Int Rpt
DIVNIDVTVIKDGYFGD AMPM_HAEIN 92 9 -
DIVNIDVTVIKDGFHGD AMPM_ECOLI 92 9 -
DIISIDIGAKLNGYHGD AMPM_BACSU 89 8 -
DIVNIDVTVIKDEFHGD AMPM_SALTY 91 9 -
DIVSIDCGVILDGFYSD AMPM_BORBU 91 8 -
DIINVDVTPIVDGYHGD AMP1_SYNY3 93 9 -
DIINIDVTLRLAGYHGD AMP2_SYNY3 115 9 -
DLVSIDCGAVLDGWHGD O33008 101 9 -
DIVNIDVTAYIGGVHGD O33343 126 8 -
DVISIDCGAIIDGWHGD O54208 90 9 -
DLVSIDCGAVLDGWHGD O53797 101 9 -
DIMNIDVSCIVDGFYGD O84859 130 8 -
DVVSIDFGAIYDGYAGD O66489 97 9 -
DLVSLDVGINLNGYISD AMPM_TREPA 88 8 -
DIVNLDVSLYYQGYHAD AMP1_YEAST 214 8 -
DLVSIDCGALLDGWAGD O69937 95 8 -
DIVNIDVSLYHNGFHGD O59730 204 8 -
DIIGLDLGVEVDGYYGD AMPM_HELPY 90 8 -
DICKIDFGTHISGRIID AMP2_HUMAN 246 11 -
DICKIDFGTHISGRIID AMP2_MOUSE 246 11 -
DICKIDFGTHISGRIID AMP2_RAT 246 11 -
DLLKVDTGAYFQGYHGD AMP3_SYNY3 142 9 -
DVVKLDVGAHIDGYIAD AMPM_ARCFU 80 11 -
DYLKIDVGVHIDGFIAD AMPM_PYRFU 77 11 -
DKLTLDIGINLNGYICD AMPM_MYCPN 89 8 -
DYLKIDLGVHIDGYIAD O58362 77 11 -
DVMKLDFGTHIDGHIVD O80587 199 11 -
DKLTLDIGIDYHGYLCD AMPM_MYCGE 89 8 -
DVMKVDYGVQVNGNIID AMP2_YEAST 189 11 -
DVCKIDFGTHIKGRIID O61540 216 11 -
DVMKVDIGVHVNGRIVD O60085 194 11 -
DKLTIDIGIELDNYYCD O52353 89 8 -
AVVKLDLGAHIDGFISD AMPM_SULSO 80 11 -

Motif 3 width=13
Element Seqn Id St Int Rpt
FSVVREYCGHGVG AMPM_HAEIN 163 54 -
FSVVREYCGHGIG AMPM_ECOLI 162 53 -
FSVVREYVGHGVG AMPM_BACSU 159 53 -
FSVVREYCGHGIG AMPM_SALTY 161 53 -
FGIVREYTGHGVG AMPM_BORBU 161 53 -
FSVVRDFVGHGIS AMP1_SYNY3 163 53 -
FSVVRDMVGHGIG AMP2_SYNY3 185 53 -
FGIVQGYGGHGIG O33008 177 59 -
YNVVRDFTGHGIG O33343 196 53 -
YGIIEDYGGHGIG O54208 167 60 -
FGIVAGYGGHGIG O53797 177 59 -
FSVVDQFVGHGVG O84859 200 53 -
FKTILRYGGHGVG O66489 167 53 -
FGVVYEYCGHGVG AMPM_TREPA 158 53 -
CSVVRTYCGHGVG AMP1_YEAST 285 54 -
YGIMEDFGGHGIG O69937 165 53 -
ISVVRTYCGHGIN O59730 280 59 -
FVPLKGFCGHGIG AMPM_HELPY 160 53 -
VKPIRNLNGHSIG AMP2_HUMAN 322 59 -
VKPIRNLNGHSIG AMP2_MOUSE 322 59 -
VKPIRNLNGHSIG AMP2_RAT 322 59 -
YTIVEEFTGHGVG AMP3_SYNY3 212 53 -
FKPIVNLTGHGLL AMPM_ARCFU 146 49 -
FKPIVNLSGHKIE AMPM_PYRFU 144 50 -
YYLLKQFGGHGCG AMPM_MYCPN 160 54 -
FNPIVNLSGHKIE O58362 144 50 -
VKSIRNLNGHSIG O80587 275 59 -
YFLVKEFGGHGCG AMPM_MYCGE 160 54 -
VKPCRNLCGHSIA AMP2_YEAST 265 59 -
IKAIRNLNGHSIS O61540 292 59 -
VKSIRNLCGHNLD O60085 270 59 -
YNVIKDFGGHGCG O52353 160 54 -
YKPIRNLGGHLIR AMPM_SULSO 147 50 -

Motif 4 width=13
Element Seqn Id St Int Rpt
VILKPGMVFTIEP AMPM_HAEIN 194 18 -
VVLKPGMTFTIEP AMPM_ECOLI 193 18 -
PRLKPGMVLAIEP AMPM_BACSU 190 18 -
VVLQPGMTFTIEP AMPM_SALTY 192 18 -
IRIQEGMVLAIEP AMPM_BORBU 193 19 -
KRLRPGMVFTIEP AMP1_SYNY3 194 18 -
LKLRPGMVFTVEP AMP2_SYNY3 216 18 -
PLLVPGSVLAIEP O33008 208 18 -
TIMQPGMTFTIEP O33343 227 18 -
PKLVPGFCLAIEP O54208 200 20 -
PLLAAGSVLAIEP O53797 208 18 -
IPLAPGMIFTIEP O84859 230 17 -
PRLRQGMVIAIEP O66489 200 20 -
PRFLPGMVVAIEP AMPM_TREPA 190 19 -
GVMKPGMVFTIEP AMP1_YEAST 316 18 -
LPLRHGMVLAIEP O69937 196 18 -
GIARPGMTFTIEP O59730 311 18 -
PKIKEGMVFCLEP AMPM_HELPY 195 22 -
TRMEEGEVYAIET AMP2_HUMAN 353 18 -
TRMEEGEVYAIET AMP2_MOUSE 353 18 -
TRMEEGEVYAIET AMP2_RAT 353 18 -
VKLKPGMTLAIEP AMP3_SYNY3 244 19 -
VTLEEGMVVAIEP AMPM_ARCFU 177 18 -
YVLKEGDVFAIEP AMPM_PYRFU 176 19 -
TKLEPGMVLCIEP AMPM_MYCPN 191 18 -
YVLQEGDVFAIEP O58362 176 19 -
TKMEEGELYAIET O80587 306 18 -
VRLQEGMVICIEP AMPM_MYCGE 191 18 -
TKMEEGEHFAIET AMP2_YEAST 296 18 -
TRMEEDEFYAIET O61540 323 18 -
IKMEEGEIFAIET O60085 301 18 -
ELLTPNMVICIEP O52353 191 18 -
GVIQSDSVYAIEP AMPM_SULSO 178 18 -