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PR00521

Identifier
ANDROGENR  [View Relations]  [View Alignment]  
Accession
PR00521
No. of Motifs
9
Creation Date
07-JUN-1996  (UPDATE 13-JUN-1999)
Title
Androgen receptor (3C nuclear receptor) signature
Database References
PRINTS; PR00398 STRDHORMONER
INTERPRO; IPR001103
Literature References
1. NUCLEAR RECEPTORS NOMENCLATURE COMMITTEE
A unified nomenclature system for the nuclear receptor superfamily.
CELL 97 161-163 (1999).
 
2. NISHIKAWA, J-I., KITAURA, M., IMAGAWA, M. AND NISHIHARA, T.
Vitamin D receptor contains multiple dimerisation interfaces that
are functionally different.
NUCLEIC ACIDS RES. 23(4) 606-611 (1995).
 
3. DE VOS, P., SCHMITT, J., VERHOEVEN, G. AND STUNNENBERG, G.
Human androgen receptor expressed in HeLa cells activates transcription
in vitro.
NUCLEIC ACIDS RES. 22(7) 1161-1166 (1994).
 
4. BATCH, J.A., WILLIAMS, D.M., DAVIES, H.R., BROWN, B.D., EVANS, B.A.J.,
HUGHES, I.A. AND PATTERSON, M.N.
Androgen receptor gene mutations identified by SSCP in fourteen subjects
with androgen insensitivity syndrome.
HUM.MOL.GENET. 1 497-503 (1992).
 
5. NAKAO, R., HAJI, M., YANASE, T., OGO, A., TAKAYANAGI, R., KATSUBE, T.,
FUKUMAKI, Y. AND NAWATA, H.
A single amino acid substitution (Met786----Val) in the steroid-binding
domain of human androgen receptor leads to complete androgen insensitivity
syndrome.
J.CLIN.ENDOCRINOL.METAB. 74 1152-1157 (1992).

Documentation
Steroid or nuclear hormone receptors (NRs) constitute an important super-
family of transcription regulators that are involved in widely diverse 
physiological functions, including control of embryonic development, cell
differentiation and homeostasis [1]. Members of the superfamily include the
steroid hormone receptors and receptors for thyroid hormone, retinoids, 
1,25-dihydroxy-vitamin D3 and a variety of other ligands. The proteins 
function as dimeric molecules in nuclei to regulate the transcription of 
target genes in a ligand-responsive manner [2,3]. In addition to C-terminal
ligand-binding domains, these nuclear receptors contain a highly-conserved,
N-terminal zinc-finger that mediates specific binding to target DNA 
sequences, termed ligand-responsive elements. In the absence of ligand,
steroid hormone receptors are thought to be weakly associated with nuclear
components; hormone binding greatly increases receptor affinity.
 
NRs are extremely important in medical research, a large number of them
being implicated in diseases such as cancer, diabetes, hormone resistance
syndromes, etc. [1]. While several NRs act as ligand-inducible transcription
factors, many do not yet have a defined ligand and are accordingly termed 
"orphan" receptors. During the last decade, more than 300 NRs have been
described, many of which are orphans, which cannot easily be named due to 
current nomenclature confusions in the literature. However, a new system 
has recently been introduced in an attempt to rationalise the increasingly 
complex set of names used to describe superfamily members [1].
 
The androgen receptor consists of 3 functional and structural domains:
an N-terminal (modulatory) domain; a DNA binding domain that mediates
specific binding to target DNA sequences (ligand-responsive elements);
and a hormone binding domain. The N-terminal domain is unique to the 
androgen receptors and spans approximately the first 530 residues; the
highly-conserved DNA-binding domain is smaller (around 65 residues) and
occupies the central portion of the protein; and the hormone binding
domain lies at the receptor C-terminus.
 
Defects in the androgen receptor cause testicular feminisation syndrome
and androgen insensibility syndrome (AIS) [4,5]. AIS may be complete (CAIS),
where external genitalia are phenotypically female; partial (PAIS), where
genitalia are substantively ambiguous; or mild (MAIS), where external
genitalia are normal male, or nearly so. Defects in the receptor also cause
X-linked spinal and bulbar muscular atrophy (also known as Kennedy's disease).
 
ANDROGENR is a 9-element fingerprint that provides a signature for the
androgen receptors. The fingerprint was derived from an initial alignment
of 3 sequences: the motifs span the N-terminal modulatory domain preceding
the highly-conserved zinc-binding region shared by the steroid hormone
receptor family. Two iterations on OWL28.0 were required to reach
convergence, at which point a true set comprising 5 sequences was
identified. Two partial matches were also found (HUMANRE and ANDR_RABIT), 
both of which are androgen receptor fragments.
 
An update on SPTR37_9f identified a true set of 4 sequences, and 3
partial matches.
Summary Information
   4 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
9444444444
8000000000
7000000000
6000111111
5000000000
4000000000
3000000000
2100000010
123456789
True Positives
ANDR_HUMAN    ANDR_MOUSE    ANDR_RAT      Q63049        
True Positive Partials
Codes involving 6 elements
Q13771
Codes involving 2 elements
P70048
Sequence Titles
ANDR_HUMAN  ANDROGEN RECEPTOR - HOMO SAPIENS (HUMAN).     
ANDR_MOUSE ANDROGEN RECEPTOR - MUS MUSCULUS (MOUSE).
ANDR_RAT ANDROGEN RECEPTOR - RATTUS NORVEGICUS (RAT).
Q63049 RAT ANDROGEN RECEPTOR - RATTUS NORVEGICUS (RAT).

Q13771 ANDROGEN RECEPTOR - HOMO SAPIENS (HUMAN).

P70048 ANDROGEN RECEPTOR ALPHA ISOFORM - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
Scan History
OWL28_0    2  25   NSINGLE    
SPTR37_9f 2 25 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
GLGRVYPRPPSKTYRGAFQN ANDR_RAT 6 6 -
GLGRVYPRPPSKTYRGAFQN ANDR_MOUSE 6 6 -
GLGRVYPRPPSKTYRGAFQN ANDR_HUMAN 6 6 -

Motif 2 width=19
Element Seqn Id St Int Rpt
FQSVREAIQNPGPRHPEAA ANDR_RAT 27 1 -
FQSVREAIQNPGPRHPEAA ANDR_MOUSE 27 1 -
FQSVREVIQNPGPRHPEAA ANDR_HUMAN 27 1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
PTFPGLSSCSADIKDILSEA ANDR_RAT 148 102 -
PTFPGLSSCSADIKDILNEA ANDR_MOUSE 148 102 -
PTFPGLSSCSADLKDILSEA ANDR_HUMAN 167 121 -

Motif 4 width=21
Element Seqn Id St Int Rpt
PSSSKDSYLGGNSTISDSAKE ANDR_RAT 214 46 -
PSSSKDSYLGGNSTISDSAKE ANDR_MOUSE 211 43 -
PTSSKDNYLGGTSTISDNAKE ANDR_HUMAN 216 29 -

Motif 5 width=21
Element Seqn Id St Int Rpt
RGDCMYASLLGGPPAVRPTPC ANDR_RAT 260 25 -
RGDCMYASLLGGPPAVRPTPC ANDR_MOUSE 257 25 -
RGDCMYAPLLGVPPAVRPTPC ANDR_HUMAN 262 25 -

Motif 6 width=21
Element Seqn Id St Int Rpt
KGTEETAEYSSFKGGYAKGLE ANDR_RAT 297 16 -
KSTEETAEYSSFKGGYAKGLE ANDR_MOUSE 294 16 -
KSTEDTAEYSPFKGGYTKGLE ANDR_HUMAN 299 16 -

Motif 7 width=23
Element Seqn Id St Int Rpt
SLYKSGAVDEAAAYQNRDYYNFP ANDR_RAT 342 24 -
SLYKSGALDEAAAYQNRDYYNFP ANDR_MOUSE 339 24 -
SLYKSGALDEAAAYQSRDYYNFP ANDR_HUMAN 344 24 -

Motif 8 width=20
Element Seqn Id St Int Rpt
HPHARIKLENPSDYGSAWAA ANDR_RAT 378 13 -
HPHARIKLENPLDYGSAWAA ANDR_MOUSE 375 13 -
HPHARIKLENPLDYGSAWAA ANDR_HUMAN 380 13 -

Motif 9 width=18
Element Seqn Id St Int Rpt
SSSWHTLFTAEEGQLYGP ANDR_RAT 428 30 -
SSSWHTLFTAEEGQLYGP ANDR_MOUSE 425 30 -
SSSWHTLFTAEEGQLYGP ANDR_HUMAN 430 30 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
GLGRVYPRPPSKTYRGAFQN ANDR_RAT 6 6 -
GLGRVYPRPPSKTYRGAFQN Q63049 6 6 -
GLGRVYPRPPSKTYRGAFQN ANDR_MOUSE 6 6 -
GLGRVYPRPPSKTYRGAFQN ANDR_HUMAN 6 6 -

Motif 2 width=19
Element Seqn Id St Int Rpt
FQSVREAIQNPGPRHPEAA ANDR_RAT 27 1 -
FQSVREAIQNPGPRHPEAA Q63049 27 1 -
FQSVREAIQNPGPRHPEAA ANDR_MOUSE 27 1 -
FQSVREVIQNPGPRHPEAA ANDR_HUMAN 27 1 -

Motif 3 width=20
Element Seqn Id St Int Rpt
PTFPGLSSCSADIKDILSEA ANDR_RAT 148 102 -
PTFPGLSSCSADIKDILSEA Q63049 148 102 -
PTFPGLSSCSADIKDILNEA ANDR_MOUSE 148 102 -
PTFPGLSSCSADLKDILSEA ANDR_HUMAN 167 121 -

Motif 4 width=21
Element Seqn Id St Int Rpt
PSSSKDSYLGGNSTISDSAKE ANDR_RAT 214 46 -
PSSSKDSYLGGNSTISDSAKE Q63049 213 45 -
PSSSKDSYLGGNSTISDSAKE ANDR_MOUSE 211 43 -
PTSSKDNYLGGTSTISDNAKE ANDR_HUMAN 216 29 -

Motif 5 width=21
Element Seqn Id St Int Rpt
RGDCMYASLLGGPPAVRPTPC ANDR_RAT 260 25 -
RGDCMYASLLGGPPAVRPTPC Q63049 259 25 -
RGDCMYASLLGGPPAVRPTPC ANDR_MOUSE 257 25 -
RGDCMYAPLLGVPPAVRPTPC ANDR_HUMAN 262 25 -

Motif 6 width=21
Element Seqn Id St Int Rpt
KGTEETAEYSSFKGGYAKGLE ANDR_RAT 297 16 -
KGTEETAEYSSFKGGYAKGLE Q63049 296 16 -
KSTEETAEYSSFKGGYAKGLE ANDR_MOUSE 294 16 -
KSTEDTAEYSPFKGGYTKGLE ANDR_HUMAN 299 16 -

Motif 7 width=23
Element Seqn Id St Int Rpt
SLYKSGAVDEAAAYQNRDYYNFP ANDR_RAT 342 24 -
SLYKSGAVDEAAAYQNRDYYNFP Q63049 341 24 -
SLYKSGALDEAAAYQNRDYYNFP ANDR_MOUSE 339 24 -
SLYKSGALDEAAAYQSRDYYNFP ANDR_HUMAN 344 24 -

Motif 8 width=20
Element Seqn Id St Int Rpt
HPHARIKLENPSDYGSAWAA ANDR_RAT 378 13 -
HPHARIKLENPSDYGSAWAA Q63049 377 13 -
HPHARIKLENPLDYGSAWAA ANDR_MOUSE 375 13 -
HPHARIKLENPLDYGSAWAA ANDR_HUMAN 380 13 -

Motif 9 width=18
Element Seqn Id St Int Rpt
SSSWHTLFTAEEGQLYGP ANDR_RAT 428 30 -
SSSWHTLFTAEEGQLYGP Q63049 427 30 -
SSSWHTLFTAEEGQLYGP ANDR_MOUSE 425 30 -
SSSWHTLFTAEEGQLYGP ANDR_HUMAN 430 30 -