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PR00481

Identifier
LAMNOPPTDASE  [View Relations]  [View Alignment]  
Accession
PR00481
No. of Motifs
5
Creation Date
20-MAR-1996  (UPDATE 07-JUN-1999)
Title
Cytosol aminopeptidase signature
Database References

PROSITE; PS00631 CYTOSOL_AP
PFAM; PF00883 Peptidase_M17
INTERPRO; IPR000819
PDB; 1BPM
SCOP; 1BPM
CATH; 1BPM
Literature References
1. BARTLING, D. AND WEILER, E.W.
Leucine aminopeptidase from Arabidopsis thaliana. Molecular evidence for a
phylogenetically conserved enzyme of protein turnover in higher polants.
EUR.J.BIOCHEMISTRY 205(1) 425-431 (1992).
 
2. BURLEY, S.K., DAVID, P.R., TAYLOR, A. AND LIPSCOMB, W.N.
Molecular structure of leucine aminopeptidase at 2.7-A resolution.
PROC.NATL.ACAD.SCI.U.S.A. 87 6878-6882 (1990).

Documentation
Aminopeptidases are exopeptidases involved in the processing and regular
turnover of intracellular proteins, although their precise role in cellular
metabolism is unclear [1,2]. Leucine aminopeptidases cleave leucine residues
from the N-terminal of polypeptide chains, but substantial rates are evident
for all amino acids [2]. 
 
The enzymes exist as homo-hexamers, comprising 2 trimers stacked on top of
one another [2]. Each monomer binds 2 zinc ions and folds into 2 alpha/beta-
type quasi-spherical globular domains, producing a comma-like shape [2]. The
N-terminal 150 residues form a 5-stranded beta-sheet with 4 parallel and 1
anti-parallel strand sandwiched between 4 alpha-helices [2]. An alpha-helix
extends into the C-terminal domain, which comprises a central 8-stranded 
saddle-shaped beta-sheet sandwiched between groups of helices, forming the
monomer hydrophobic core [2]. A 3-stranded beta-sheet resides on the surface
of the monomer, where it interacts with other members of the hexamer [2].
The 2 zinc ions and the active site are entirely located in the C-terminal
catalytic domain [2].
 
LAMNOPPTDASE is a 5-element fingerprint that provides a signature for
leucine aminopeptidases. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from the vicinity of the
active site in the C-terminal catalytic domain, motif 4 including the
region encoded by PROSITE pattern CYTOSOL_AP (PS00631), the aspartate and
glutamate of which are involved in zinc ion-binding. Two iterations on
OWL27.0 were required to reach convergence, at which point a true set
comprising 16 sequences was identified.
 
An update on SPTR37_9f identified a true set of 27 sequences.
Summary Information
27 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
52727272727
400000
300000
200000
12345
True Positives
AMP1_LYCES    AMP2_LYCES    AMPA_ECOLI    AMPA_HAEIN    
AMPA_HELPY AMPL_ARATH AMPL_BOVIN AMPL_MYCGE
AMPL_MYCPN AMPL_MYCSA AMPL_RICPR AMPL_SOLTU
AMPL_SYNY3 O24022 O32106 O32956
O65557 O67868 O68822 O84049
O86436 PEPB_ECOLI PEPB_MYCTU Q27245
Q96476 YA55_SCHPO YOJ6_CAEEL
Sequence Titles
AMP1_LYCES  CHLOROPLAST AMINOPEPTIDASE 1 PRECURSOR (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) (LEUCYL AMINOPEPTIDASE) (PROLINE AMINOPEPTIDASE) (EC 3.4.11.5) (PROLYL AMINOPEPTIDASE) - LYCOPERSICON ESCULENTUM (TOMATO). 
AMP2_LYCES CHLOROPLAST AMINOPEPTIDASE 2 PRECURSOR (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) (LEUCYL AMINOPEPTIDASE) (PROLINE AMINOPEPTIDASE) (EC 3.4.11.5) (PROLYL AMINOPEPTIDASE) - LYCOPERSICON ESCULENTUM (TOMATO).
AMPA_ECOLI AMINOPEPTIDASE A/I (EC 3.4.11.1) - ESCHERICHIA COLI.
AMPA_HAEIN AMINOPEPTIDASE A/I (EC 3.4.11.1) - HAEMOPHILUS INFLUENZAE.
AMPA_HELPY AMINOPEPTIDASE A/I (EC 3.4.11.1) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
AMPL_ARATH CYTOSOL AMINOPEPTIDASE (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) (LEUCYL AMINOPEPTIDASE) (PROLINE AMINOPEPTIDASE) (EC 3.4.11.5) (PROLYL AMINOPEPTIDASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
AMPL_BOVIN CYTOSOL AMINOPEPTIDASE (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) (LEUCYL AMINOPEPTIDASE) (PROLINE AMINOPEPTIDASE) (EC 3.4.11.5) (PROLYL AMINOPEPTIDASE) - BOS TAURUS (BOVINE).
AMPL_MYCGE PROBABLE CYTOSOL AMINOPEPTIDASE (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) - MYCOPLASMA GENITALIUM.
AMPL_MYCPN PROBABLE CYTOSOL AMINOPEPTIDASE (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) - MYCOPLASMA PNEUMONIAE.
AMPL_MYCSA PROBABLE CYTOSOL AMINOPEPTIDASE (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) - MYCOPLASMA SALIVARIUM.
AMPL_RICPR CYTOSOL AMINOPEPTIDASE (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) (LEUCYL AMINOPEPTIDASE) (PROLINE AMINOPEPTIDASE (EC 3.4.11.5) (PROLYL AMINOPEPTIDASE)) - RICKETTSIA PROWAZEKII.
AMPL_SOLTU CHLOROPLAST AMINOPEPTIDASE PRECURSOR (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) (LEUCYL AMINOPEPTIDASE) (PROLINE AMINOPEPTIDASE) (EC 3.4.11.5) (PROLYL AMINOPEPTIDASE) - SOLANUM TUBEROSUM (POTATO).
AMPL_SYNY3 PUTATIVE CYTOSOL AMINOPEPTIDASE (EC 3.4.11.1) (LEUCINE AMINOPEPTIDASE) (LAP) (LEUCYL AMINOPEPTIDASE) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
O24022 LAP17.1A PROTEIN - LYCOPERSICON ESCULENTUM (TOMATO).
O32106 YUIE PROTEIN - BACILLUS SUBTILIS.
O32956 AMINOPEPTIDASE - MYCOBACTERIUM LEPRAE.
O65557 LEUCYL AMINOPEPTIDASE - LIKE PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O67868 LEUCINE AMINOPEPTIDASE - AQUIFEX AEOLICUS.
O68822 PHPA - PSEUDOMONAS AERUGINOSA.
O84049 LEUCYL AMINOPEPTIDASE A - CHLAMYDIA TRACHOMATIS.
O86436 LEUCINE AMINOPEPTIDASE (EC 3.4.11.1) - PSEUDOMONAS PUTIDA.
PEPB_ECOLI PEPTIDASE B (EC 3.-.-.-) - ESCHERICHIA COLI.
PEPB_MYCTU PEPTIDASE B (EC 3.-.-.-) - MYCOBACTERIUM TUBERCULOSIS.
Q27245 W07G4.4 PROTEIN - CAENORHABDITIS ELEGANS.
Q96476 LAP17.1A PROTEIN - LYCOPERSICON ESCULENTUM (TOMATO).
YA55_SCHPO PUTATIVE AMINOPEPTIDASE C13A11.05 IN CHROMOSOME I (EC 3.4.11.-) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
YOJ6_CAEEL PUTATIVE AMINOPEPTIDASE ZK353.6 IN CHROMOSOME III (EC 3.4.11.-) - CAENORHABDITIS ELEGANS.
Scan History
OWL27_0    2  100  NSINGLE    
SPTR37_9f 2 28 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
IVLVGKGLTFDAGGISLK AMPA_HAEIN 258 258 -
LVFVGKGITFDSGGISIK AMPL_BOVIN 245 245 -
LGLVGKGVTFDSGGISIK YA55_SCHPO 275 275 -
IALVGKGVVYDTGGLQIK YOJ6_CAEEL 247 247 -
LVFVGKGITFDSGGISIK NRL_1BPM 242 242 -
LALVGKGLTFDSGGYNIK AMPL_ARATH 283 283 -
IVLVGKGLTFDSGGISIK AMPA_ECOLI 265 265 -

Motif 2 width=22
Element Seqn Id St Int Rpt
MDEMKYDMCGAASVFGTMKTIA AMPA_HAEIN 280 4 -
MKEMRADMGGAAVMLSSIYALE YA55_SCHPO 297 4 -
MDLMRADMGGAATICSAIVSAA NRL_1BPM 264 4 -
MPNMKRDMGGAAGMLEAYSALV YOJ6_CAEEL 269 4 -
IELMKFDMGGSAAVLGAAKAIG AMPL_ARATH 307 6 -
MDEMKYDMCGAAAVYGVMRMVA AMPA_ECOLI 287 4 -
MDLMRADMGGAATICSAIVSAA AMPL_BOVIN 267 4 -

Motif 3 width=22
Element Seqn Id St Int Rpt
ENMISGTGMRPGDVITASNGKT AMPL_ARATH 345 16 -
ENMPSGKANKPGDVVRARNGKT NRL_1BPM 301 15 -
ENMPGGRAYRPGDVLTTMSGQT AMPA_ECOLI 324 15 -
ENLPDGNAYRPGDILTTMNGLT AMPA_HAEIN 317 15 -
ENMPSGKANKPGDVVRARNGKT AMPL_BOVIN 304 15 -
ENLPSGSAAKPGDVIFMRNGLS YA55_SCHPO 334 15 -
ENNVSPIANKPDDIIKMLSGKT YOJ6_CAEEL 306 15 -

Motif 4 width=21
Element Seqn Id St Int Rpt
VEINNTDAEGRLILADGVFYA YOJ6_CAEEL 328 0 -
IEVNNTDAEGRLTLADALVYA AMPL_ARATH 367 0 -
VEVLNTDAEGRLVLCDVLTYV AMPA_ECOLI 346 0 -
IQVDNTDAEGRLILADALCYA NRL_1BPM 323 0 -
VEVLNTDAEGRLVLCDTLTYV AMPA_HAEIN 339 0 -
IQVDNTDAEGRLILADALCYA AMPL_BOVIN 326 0 -
VEIDNTDAEGRLILADAVHYV YA55_SCHPO 356 0 -

Motif 5 width=16
Element Seqn Id St Int Rpt
IINAATLTGAMDIALG AMPL_BOVIN 354 7 -
IFDMATLTGAQAWLSG YOJ6_CAEEL 357 8 -
IVDLATLTGACVIALG AMPL_ARATH 395 7 -
IINAATLTGAMDIALG NRL_1BPM 351 7 -
VIDVATLTGACVIALG AMPA_ECOLI 374 7 -
VIDVATLTGACVVALG AMPA_HAEIN 367 7 -
VIEASTLTGAMLVALG YA55_SCHPO 385 8 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
LALVGKGLTFDSGGYNLK AMP1_LYCES 337 337 -
LALVGKGLTFDSGGYNLK O24022 337 337 -
LALVGKGLTFDSGGYNLK Q96476 350 350 -
IALVGKGLTFDSGGYNLK AMPL_SOLTU 337 337 -
LALVGKGLTFDSGGYNIK AMPL_ARATH 283 283 -
IALVGKGLTFDSGGYNIK AMP2_LYCES 334 334 -
HVLVGKGITFDTGGISLK O68822 261 261 -
LALVGKMVFVHSGGYNIK O65557 288 288 -
FVLVGKGITFDTGGISLK O86436 262 262 -
IVLVGKGLTFDSGGISIK AMPA_ECOLI 265 265 -
IVLVGKGLTFDAGGISLK AMPA_HAEIN 258 258 -
LAIIGKSLTFDSGGLNIK AMPL_SYNY3 270 270 -
IALVGKGLTFDSGGLNIK O67868 252 252 -
LALVGKGVIFDTGGISLK AMPL_RICPR 260 260 -
VGLVGKGITFDTGGYSIK O32106 256 256 -
LVFVGKGITFDSGGISIK AMPL_BOVIN 245 245 -
LALVGKGITYDSGGMNIK AMPL_MYCPN 212 212 -
VALVGKGITFDTGGISIK O32956 283 283 -
VALVGKGITFDTGGISIK PEPB_MYCTU 274 274 -
ACLVGKGITFDSGGYSIK PEPB_ECOLI 190 190 -
IALVGKGLTYDCGGLSLK AMPA_HELPY 253 253 -
LAFVGKGITYDSGGMNIK AMPL_MYCGE 213 213 -
TVLVGKGITFDSGGYSLK AMPL_MYCSA 227 227 -
LGLVGKGVTFDSGGISIK YA55_SCHPO 275 275 -
TVLIGKGVTFDSGGLDLK O84049 258 258 -
IALVGKGVVYDTGGLQIK YOJ6_CAEEL 247 247 -
FFVVGKGVTIDTGGCDLK Q27245 266 266 -

Motif 2 width=22
Element Seqn Id St Int Rpt
IELMKNDMGGAAAVLGAAKALG AMP1_LYCES 361 6 -
IELMKNDMGGAAAVLGAAKALG O24022 361 6 -
IELMKNDMGGAAAVLGAAKALG Q96476 374 6 -
IELMKNDMGGAAAVLGAAKALG AMPL_SOLTU 361 6 -
IELMKFDMGGSAAVLGAAKAIG AMPL_ARATH 307 6 -
IELMKFDMGGAAAVLGAAKALG AMP2_LYCES 358 6 -
MDEMKFDMCGAASVFGTFRAVL O68822 283 4 -
IELMKFDMGGSAAVLGAAKAIG O65557 312 6 -
MDEMKYDMCGAASVFGTLRAVL O86436 284 4 -
MDEMKYDMCGAAAVYGVMRMVA AMPA_ECOLI 287 4 -
MDEMKYDMCGAASVFGTMKTIA AMPA_HAEIN 280 4 -
IETMKMDMGGGGATLGAAKAIA AMPL_SYNY3 293 5 -
MRTMKMDKSGACAVLGIMRAIA O67868 274 4 -
MHLMRYDMAGSAAVVGTIIALA AMPL_RICPR 282 4 -
IVGMKSDMGGAAAVLGAMETIG O32106 278 4 -
MDLMRADMGGAATICSAIVSAA AMPL_BOVIN 267 4 -
MRGMKYDMSGAAIVCSTVLALA AMPL_MYCPN 234 4 -
MHHMTSDMAGAAAVIATITLAA O32956 305 4 -
MHHMTSDMGGAAAVIATVTLAA PEPB_MYCTU 296 4 -
MDSMKSDMGGAATVTGALAFAI PEPB_ECOLI 212 4 -
MVTMKADKGGGSAVIGLLNALA AMPA_HELPY 275 4 -
MRGMKYDMSGAAIVCSTVLALA AMPL_MYCGE 235 4 -
MLGMKYDMSGSAIVAAVMKAIA AMPL_MYCSA 249 4 -
MKEMRADMGGAAVMLSSIYALE YA55_SCHPO 297 4 -
MITMKEDMAGAATVLGIFSALA O84049 280 4 -
MPNMKRDMGGAAGMLEAYSALV YOJ6_CAEEL 269 4 -
MFGMCRDKYGSAVVGGFFKAID Q27245 288 4 -

Motif 3 width=22
Element Seqn Id St Int Rpt
ENMISAEGMRPGDIVTASNGKT AMP1_LYCES 399 16 -
ENMISAEGMRPGDIVTASNGKT O24022 399 16 -
ENMISAEGMRPGDIVTASNGKT Q96476 412 16 -
ENMISGAGMRPGDIVTASNGKT AMPL_SOLTU 399 16 -
ENMISGTGMRPGDVITASNGKT AMPL_ARATH 345 16 -
ENMISGTGMRPGDIITASNGKT AMP2_LYCES 396 16 -
ENMPSGGATRPGDIVTTMSGQT O68822 320 15 -
ENMISGTGMRPGDVLTASNGKT O65557 350 16 -
ENMPSGGATRPGDIVTTMSGQT O86436 321 15 -
ENMPGGRAYRPGDVLTTMSGQT AMPA_ECOLI 324 15 -
ENLPDGNAYRPGDILTTMNGLT AMPA_HAEIN 317 15 -
ENMISGTAMHPGDILTASNGKT AMPL_SYNY3 330 15 -
ENMPDGNAYRPDDVIKAKNGKY O67868 311 15 -
ENMQSGNAQRPGDVVVTMSGQT AMPL_RICPR 319 15 -
DNMISGGAMKPDDVIVSLSGKT O32106 315 15 -
ENMPSGKANKPGDVVRARNGKT AMPL_BOVIN 304 15 -
ENLPGPHAQRPDDIQTAYNGKT AMPL_MYCPN 271 15 -
ENMPSGTAQRPGDVLTQYGGIT O32956 342 15 -
ENMPSATAQRPGDVLTQYGGTT PEPB_MYCTU 333 15 -
DNLISGNAFKLGDIITYRNGKK PEPB_ECOLI 249 15 -
ENMIGPAAYKPDDILISKEGKS AMPA_HELPY 312 15 -
ENLPGAKAQRPDDIKIAYNGKS AMPL_MYCGE 272 15 -
DNRINGDASLPDSVYTSMSGKT AMPL_MYCSA 286 15 -
ENLPSGSAAKPGDVIFMRNGLS YA55_SCHPO 334 15 -
ENAIGSAAYKMGDVYVGMTGLS O84049 317 15 -
ENNVSPIANKPDDIIKMLSGKT YOJ6_CAEEL 306 15 -
RNSIGSHAYTCDEVITSRSGKR Q27245 326 16 -

Motif 4 width=21
Element Seqn Id St Int Rpt
IEVNNTDAEGRLTLADALIYA AMP1_LYCES 421 0 -
IEVNNTDAEGRLTLADALIYA O24022 421 0 -
IEVNNTDAEGRLTLADALIYA Q96476 434 0 -
IEVNNTDAEGRLTLADALIYA AMPL_SOLTU 421 0 -
IEVNNTDAEGRLTLADALVYA AMPL_ARATH 367 0 -
IEVNNTDAEGRLTLSVGISCN AMP2_LYCES 418 0 -
VEILNTDAEGRLVLCDALTYA O68822 342 0 -
IEVNNTDAEGRLTLADALVYA O65557 372 0 -
VEILNTDAEGRLVLCDTLTYA O86436 343 0 -
VEVLNTDAEGRLVLCDVLTYV AMPA_ECOLI 346 0 -
VEVLNTDAEGRLVLCDTLTYV AMPA_HAEIN 339 0 -
IEVNNTDAEGRLTLADALVFA AMPL_SYNY3 352 0 -
IEIDNTDAEGRVTLADVLSYA O67868 333 0 -
AEVLNTDAEGRLVLADTVWYV AMPL_RICPR 341 0 -
IEILNTDAEGRLVLADGITYA O32106 337 0 -
IQVDNTDAEGRLILADALCYA AMPL_BOVIN 326 0 -
VEIDNTDAEGRLVLADAISYA AMPL_MYCPN 293 0 -
VEVLNTDAEGRLILADAIVRA O32956 364 0 -
VEVLNTDAEGRLILADAIVRA PEPB_MYCTU 355 0 -
VEVMNTDAEGRLVLADGLIDA PEPB_ECOLI 271 0 -
IEVRNTDAEGRLVLADCLSYA AMPA_HELPY 334 0 -
VEIDNTDAEGRLVLADAITYA AMPL_MYCGE 294 0 -
VEVNNTDAEGRLVLADRLYYG AMPL_MYCSA 308 0 -
VEIDNTDAEGRLILADAVHYV YA55_SCHPO 356 0 -
VEIGSTDAEGRLILADAISYA O84049 339 0 -
VEINNTDAEGRLILADGVFYA YOJ6_CAEEL 328 0 -
IHIYNTDAEGRLTMLDPLTLA Q27245 348 0 -

Motif 5 width=16
Element Seqn Id St Int Rpt
IIDLATLTGAIMVALG AMP1_LYCES 449 7 -
IIDLATLTGAIMVALG O24022 449 7 -
IIDLATLTGAIMVALG Q96476 462 7 -
IIDLATLTGAIVTALG AMPL_SOLTU 449 7 -
IVDLATLTGACVIALG AMPL_ARATH 395 7 -
IVDLATLTGACVVALG AMP2_LYCES 444 5 -
VIDIATLTGACIVALG O68822 370 7 -
VVDLATLTGACIIALG O65557 400 7 -
VIDIATLTGACIVALG O86436 371 7 -
VIDVATLTGACVIALG AMPA_ECOLI 374 7 -
VIDVATLTGACVVALG AMPA_HAEIN 367 7 -
IVDLATLTGACIVALG AMPL_SYNY3 380 7 -
IIDMATLTGACMVALG O67868 361 7 -
VIDVATLTGAITVALG AMPL_RICPR 370 8 -
LVDVATLTGGVVVALG O32106 365 7 -
IINAATLTGAMDIALG AMPL_BOVIN 354 7 -
IIDVATLTGLMSYILS AMPL_MYCPN 322 8 -
LIETSTLTGAQTVALG O32956 392 7 -
LIETSTLTGAQTVALG PEPB_MYCTU 383 7 -
IIDAATLTGAAKTALG PEPB_ECOLI 299 7 -
IVDFATLTGACVVGLG AMPA_HELPY 362 7 -
IIDVATLTGLMSYILS AMPL_MYCGE 323 8 -
LIDTATLTGTMLTALG AMPL_MYCSA 337 8 -
VIEASTLTGAMLVALG YA55_SCHPO 385 8 -
IIDFATLTGAMVVSLG O84049 368 8 -
IFDMATLTGAQAWLSG YOJ6_CAEEL 357 8 -
LFTVATLTGHEVLSYG Q27245 380 11 -