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PR00473

Identifier
GALCTOKINASE  [View Relations]  [View Alignment]  
Accession
PR00473
No. of Motifs
4
Creation Date
14-FEB-1996  (UPDATE 12-FEB-2000)
Title
Galactokinase signature
Database References

PROSITE; PS00106 GALACTOKINASE
PFAM; PF00288 GHMP_kinases
INTERPRO; IPR000705
Literature References
1. LEE, R.T., PETERSON, C.L., CALMAN, A.F., HERSKOWITZ, I.
AND O'DONNELL, J.J.
Cloning of a human galactokinase gene (GK2) on chromosome 15 by
complementation in yeast.
PROC.NATL.ACAD.SCI.U.S.A. 89 10887-10891 (1992).
 
2. BAJWA, W., TORCHIA, T.E. AND HOPPER, J.E.
Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common
with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly 
similar to yeast and Escherichia coli galactokinases.
MOL.CELL.BIOL. 8 3439-3447 (1988).

Documentation
Galactokinase catalyses the first reaction in the galactose metabolism
pathway, the ATP-dependent phosphorylation of galactose, yielding
galactose-1-phosphate [1,2]. Deficiency in this enzyme results in the
disease galactosemia, which is responsible for the formation of cataracts
in newborn babies, and is possibly responsible for presenile cataracts in
adults [1].
 
In yeast, the GAL3 gene product is required for the GAL4-mediated induction 
of other enzymes involved in galactose metabolism [2]. The induction of 
GAL1 production then reinforces this process, increasing the expression of
other galactose-inducible genes [2]. GAL3 has been shown to be similar to 
the GAL1 protein [2].
 
GALCTOKINASE is a 4-element fingerprint that provides a signature for the
galactokinase family. The fingerprint was derived from an initial alignment
of 6 sequences: the motifs were drawn mainly from the N-terminal portion of
the alignment, motif 1 including the region encoded by PROSITE pattern 
GALACTOKINASE (PS00106), and motif 3 encoding a possible ATP-binding site.
Two iterations on OWL27.0 were required to reach convergence, at which point
a true set comprising 15 sequences was identified. Two partial matches were 
also found: KLGAL is a galactokinase fragment; and S78870 is a galactokinase
from Streptococcus thermophilus F410 that fails to match motif 1. 
 
An update on SPTR37_10f identified a true set of 20 sequences, and 5
partial matches.
Summary Information
  20 codes involving  4 elements
3 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
420202020
33033
20220
1234
True Positives
GAL1_ACTPL    GAL1_BACSU    GAL1_CANAL    GAL1_CANPA    
GAL1_ECOLI GAL1_HAEIN GAL1_HUMAN GAL1_KLULA
GAL1_LACHE GAL1_SALTY GAL1_STRLI GAL1_STRMU
GAL1_YEAST GAL2_HUMAN GAL3_YEAST O21998
O58107 O84902 O85253 Q9ZB10
True Positive Partials
Codes involving 3 elements
O01969 O24021 O48936
Codes involving 2 elements
O23461 O23723
Sequence Titles
GAL1_ACTPL  GALACTOKINASE (EC 2.7.1.6) - ACTINOBACILLUS PLEUROPNEUMONIAE (HAEMOPHILUS PLEUROPNEUMONIAE). 
GAL1_BACSU GALACTOKINASE (EC 2.7.1.6) - BACILLUS SUBTILIS.
GAL1_CANAL GALACTOKINASE (EC 2.7.1.6) - CANDIDA ALBICANS (YEAST).
GAL1_CANPA GALACTOKINASE (EC 2.7.1.6) - CANDIDA PARAPSILOSIS (YEAST).
GAL1_ECOLI GALACTOKINASE (EC 2.7.1.6) - ESCHERICHIA COLI.
GAL1_HAEIN GALACTOKINASE (EC 2.7.1.6) - HAEMOPHILUS INFLUENZAE.
GAL1_HUMAN GALACTOKINASE 1 (EC 2.7.1.6) - HOMO SAPIENS (HUMAN).
GAL1_KLULA GALACTOKINASE (EC 2.7.1.6) - KLUYVEROMYCES LACTIS (YEAST).
GAL1_LACHE GALACTOKINASE (EC 2.7.1.6) - LACTOBACILLUS HELVETICUS.
GAL1_SALTY GALACTOKINASE (EC 2.7.1.6) - SALMONELLA TYPHIMURIUM.
GAL1_STRLI GALACTOKINASE (EC 2.7.1.6) - STREPTOMYCES LIVIDANS.
GAL1_STRMU GALACTOKINASE (EC 2.7.1.6) - STREPTOCOCCUS MUTANS.
GAL1_YEAST GALACTOKINASE (EC 2.7.1.6) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
GAL2_HUMAN GALACTOKINASE 2 (EC 2.7.1.6) - HOMO SAPIENS (HUMAN).
GAL3_YEAST GAL3 PROTEIN - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
O21998 GALACTOKINASE - BACTERIOPHAGE LAMBDA.
O58107 350AA LONG HYPOTHETICAL GALACTOKINASE - PYROCOCCUS HORIKOSHII.
O84902 GALACTOKINASE (EC 2.7.1.6) - LACTOBACILLUS CASEI.
O85253 GALACTOKINASE (EC 2.7.1.6) - THERMOTOGA NEAPOLITANA.
Q9ZB10 GALACTOKINASE (EC 2.7.1.6) - STREPTOCOCCUS THERMOPHILUS.

O01969 SIMILAR TO GALACTOKINASE - CAENORHABDITIS ELEGANS.
O24021 GALACTOKINASE (EC 2.7.1.6) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O48936 GALACTOKINASE (EC 2.7.1.6) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).

O23461 HYPOTHETICAL PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23723 PUTATIVE ARABINOSE KINASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Scan History
OWL27_0    2  100  NSINGLE    
SPTR37_9f 3 24 NSINGLE
SPTR37_10f 3 40 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
GRVNLIGEHTDYNDGFVLP GAL1_ECOLI 26 26 -
GRINVIGEHTDYNGGHVFP GAL1_LACHE 26 26 -
GRVNLIGEHIDYCQFSVLP GAL1_KLULA 42 42 -
GRVNLIGEHTDYNGGHVFP GAL1_BACSU 26 26 -
GRVNLIGEHIDYCDFSVLP GAL3_YEAST 46 46 -
GRVNIIGEHIDYCGYSVLP GAL2_HUMAN 42 42 -

Motif 2 width=12
Element Seqn Id St Int Rpt
WANYVRGVVKHL GAL1_ECOLI 91 46 -
WANYFKGMITYL GAL1_LACHE 92 47 -
WSNYFKCGLLVA GAL1_KLULA 114 53 -
WANYPKGVIYEF GAL1_BACSU 91 46 -
WSNYFKCGLHVA GAL3_YEAST 117 52 -
WHNYFLCGLKGI GAL2_HUMAN 107 46 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GVDMVISGNVPQGAGLSSS GAL1_ECOLI 111 8 -
GFNLYIEANLPSGSGLSSS GAL1_LACHE 113 9 -
GMEIYVKGDIPSGGGLSSS GAL1_KLULA 141 15 -
GFDIVFSGNIPNGAGLSSS GAL1_BACSU 112 9 -
GAQIFCQSDIPTGGGLSSA GAL3_YEAST 147 18 -
GMNCLVDGNIPPSSGLSSS GAL2_HUMAN 129 10 -

Motif 4 width=15
Element Seqn Id St Int Rpt
KRVRHILTENARTVE GAL1_ECOLI 262 132 -
KRARHAVSENQRTLR GAL1_LACHE 270 138 -
QRAKHVYSEALRVLK GAL1_KLULA 375 215 -
RRAKHAVYENHRAIK GAL1_BACSU 269 138 -
QRAKHVYSESLRVLK GAL3_YEAST 387 221 -
QRAKHVYSEAARVLQ GAL2_HUMAN 330 182 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
GRINLIGEHTDYNGGRVFP GAL1_STRMU 26 26 -
GRVNIIGEHTDYNDGFVMP GAL1_HAEIN 27 27 -
GRVNLIGEHTDYNDGFVLP GAL1_ECOLI 26 26 -
GRVNLIGEHTDYNDGFVLP O21998 27 27 -
GRVNLIGEHTDYNDGFVLP GAL1_SALTY 27 27 -
GRVNIIGEHTDYNDGFVMP GAL1_ACTPL 27 27 -
GRVNLIGEHIDYCDFSVLP GAL1_YEAST 51 51 -
GRINVIGEHTDYNGGHVFP GAL1_LACHE 26 26 -
GRVNLIGEHIDYCQFSVLP GAL1_KLULA 42 42 -
GRVNLIGDHIDYNFFPVLP GAL1_CANAL 47 47 -
GRVNLIGDHIDYNYFPVLP GAL1_CANPA 46 46 -
GRVNLIGEHTDYNGGHVFP GAL1_BACSU 26 26 -
GRINLIGEHTDYNGGNVLP Q9ZB10 26 26 -
GRVNLIGEHIDYCDFSVLP GAL3_YEAST 46 46 -
GRVNLIGEHTDYNQGLVLP GAL1_HUMAN 36 36 -
GRINLIGEHTDYNGGHVFP O84902 26 26 -
GRINIIGEHTDYNDGYVLP O85253 7 7 -
GRVNIIGEHIDYCGYSVLP GAL2_HUMAN 42 42 -
GRVNLIGEHTDYTYGYVMP O58107 8 8 -
GRENLIGEHTDYNDGFVMP GAL1_STRLI 29 29 -

Motif 2 width=12
Element Seqn Id St Int Rpt
WTNYAKGVIKYL GAL1_STRMU 91 46 -
WANYVRGVVKFI GAL1_HAEIN 90 44 -
WANYVRGVVKHL GAL1_ECOLI 91 46 -
WANYVRGVVKHL O21998 92 46 -
WSNYVRGVVKHL GAL1_SALTY 92 46 -
WANYVRGVVKYI GAL1_ACTPL 90 44 -
WSNYFKCGLHVA GAL1_YEAST 122 52 -
WANYFKGMITYL GAL1_LACHE 92 47 -
WSNYFKCGLLVA GAL1_KLULA 114 53 -
WANYFKCALIVA GAL1_CANAL 117 51 -
WANYFKCGLIVA GAL1_CANPA 113 48 -
WANYPKGVIYEF GAL1_BACSU 91 46 -
WTNYPKGVLHFL Q9ZB10 91 46 -
WSNYFKCGLHVA GAL3_YEAST 117 52 -
WANYVKGVIQYY GAL1_HUMAN 106 51 -
WTDYFQGMARVM O84902 90 45 -
WTDYISGVIKAF O85253 64 38 -
WHNYFLCGLKGI GAL2_HUMAN 107 46 -
WIDYVKGIFWVL O58107 67 40 -
WTAYPSGVLWAL GAL1_STRLI 92 44 -

Motif 3 width=19
Element Seqn Id St Int Rpt
GMDVYVFGNIPNGSGLSSS GAL1_STRMU 112 9 -
GANLVISGNVPLSSGLSSS GAL1_HAEIN 111 9 -
GVDMVISGNVPQGAGLSSS GAL1_ECOLI 111 8 -
GVDMVDHGNVPQGAGLSSS O21998 112 8 -
GGVDMVISNVPQGAGLSSS GAL1_SALTY 111 7 -
GADLAMTSDVPMSSGLSSS GAL1_ACTPL 111 9 -
GLQVFCEGDVPTGSGLSSS GAL1_YEAST 152 18 -
GFNLYIEANLPSGSGLSSS GAL1_LACHE 113 9 -
GMEIYVKGDIPSGGGLSSS GAL1_KLULA 141 15 -
GMKLTFDGNVPTGGGLSSS GAL1_CANAL 143 14 -
GMNITFSGTVPTGGGLSSS GAL1_CANPA 138 13 -
GFDIVFSGNIPNGAGLSSS GAL1_BACSU 112 9 -
GMDIYIYGNIPNGSGLSSS Q9ZB10 112 9 -
GAQIFCQSDIPTGGGLSSA GAL3_YEAST 147 18 -
GFSAVVVSSVPLGGGLSSS GAL1_HUMAN 124 6 -
GLNVYINGNLPDGAGLSSS O84902 111 9 -
PVKISVSSNLPMGAGLSSS O85253 84 8 -
GMNCLVDGNIPPSSGLSSS GAL2_HUMAN 129 10 -
GIKGRVSGNLPLGAGLSSS O58107 87 8 -
GADVHLASTVPSGAGLSSS GAL1_STRLI 112 8 -

Motif 4 width=15
Element Seqn Id St Int Rpt
KRARHAVSENQRTLK GAL1_STRMU 269 138 -
KRARHVVTENQRVLD GAL1_HAEIN 265 135 -
KRVRHILTENARTVE GAL1_ECOLI 262 132 -
KRVRHILTENARTVE O21998 263 132 -
KRVRHVLSENARTVE GAL1_SALTY 261 131 -
KRAKHIISENQRVLE GAL1_ACTPL 265 135 -
QRAKHVYSESLRVLK GAL1_YEAST 391 220 -
KRARHAVSENQRTLR GAL1_LACHE 270 138 -
QRAKHVYSEALRVLK GAL1_KLULA 375 215 -
QRAKHVYQESLRVLE GAL1_CANAL 372 210 -
ERALHVYRESLRVLK GAL1_CANPA 366 209 -
RRAKHAVYENHRAIK GAL1_BACSU 269 138 -
KRARHVVLENQRTLQ Q9ZB10 269 138 -
QRAKHVYSESLRVLK GAL3_YEAST 387 221 -
RRARHVVGEIRRTAQ GAL1_HUMAN 276 133 -
KRARHAVFENQRTLK O84902 268 138 -
KRAQHVLEENERVLK O85253 232 129 -
QRAKHVYSEAARVLQ GAL2_HUMAN 330 182 -
KFFGYIVRENARVLE O58107 234 128 -
RLVRHVVTEDERVER GAL1_STRLI 267 136 -