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PR00412

Identifier
EPOXHYDRLASE  [View Relations]  [View Alignment]  
Accession
PR00412
No. of Motifs
6
Creation Date
07-AUG-1995  (UPDATE 14-JUN-1999)
Title
Epoxide hydrolase signature
Database References

INTERPRO; IPR000639
UMBBD; e0033; e0397; e0003
PDB; 2DHC
SCOP; 2DHC
Literature References
1. OLLIS, D.L., CHEAH, E., CYGLER, M., DIJKSTRA, B., FROLOW, F., FRANKEN,
S.M., HAREL, M., REMINGTON, S.J., SILMAN, I., SCHRAG, J., SUSSMAN, J.L.,
VERSCHUEREN, K.H.G. AND GOLDMAN, A.
The alpha/beta hydrolase fold.
PROTEIN ENG. 5(3) 197-211 (1992).
  
2. BEETHAM, J.K., GRANT, D.F., ARAND, M., GARBARINO, J., KIYOSUE, T., 
PINOT, F., OESCH, F., BELKNAP, W.R., SHINOZAKI, K. AND HAMMOCK, B.D. 
Gene evolution of epoxide hydrolases and recommended nomenclature.
DNA CELL BIOL. 14(1) 61-71 (1995).
 
3. FRANKEN, S.M., ROZEBOOM, H.J., KALK, K.H. AND DIJKSTRA, B.W.
Crystal structure of haloalkane dehalogenase: an enzyme to detoxify 
halogenated alkanes.
EMBO J. 10 1297-1302 (1991).
 
4. VERSCHUEREN, K.H.G., SELJEE, F., ROZEBOOM, H.J., KALK, K.H. AND
DIJKSTRA, B.W.
Crystallographic analysis of the catalytic mechanism of haloalkane
dehalogenase.
NATURE 363 693-698 (1993).
 
5. PINOT, F., GRANT, D.F., BEETHAM, J.K., PARKER, A.G., BORHAN, B., 
LANDT, S., JONES, A.D. AND HAMMOCK, B.D.
Molecular and biochemical evidence for the involvement of the Asp-333-
His-523 pair in the catalytic mechanism of soluble epoxide hydrolase. 
J.BIOL.CHEM. 270 7968-7974 (1995).

Documentation
The alpha/beta hydrolase fold is common to a number of hydrolytic enzymes
of widely differing phylogenetic origin and catalytic function. The core
of each enzyme is an alpha/beta-sheet (rather than a barrel), containing 8
strands connected by helices [1]. The enzymes are believed to have diverged
from a common ancestor, preserving the arrangement of the catalytic
residues. All have a catalytic triad, the elements of which are borne on
loops, which are the best conserved structural features of the fold. 
 
The epoxide hydrolases (EH) add water to epoxides, forming the corresponding
diol. On the basis of sequence similarity, it has been proposed that the
mammalian soluble EHs contain 2 evolutionarily distinct domains [2]: the 
N-terminal domain is similar to bacterial haloacid dehalogenase; while the
C-terminal domain is similar to soluble plant EH, microsomal EH, and
bacterial haloalkane dehalogenase (HLD). 
 
The crystal structure of HLD has been resolved to 1.9A [3]. The enzyme 
contains 2 domains: domain I, has an alpha/beta type structure with a
central 8-stranded beta-sheet, and contains the nucleophile-acid-His
catalytic triad; in the linear sequence, domain I is interrupted by domain 
II (residues 156-229), which consists of alpha-helices connected by loops,
and is responsible for substrate binding and specificity. The similarity of 
EH to HLD is important for deducing a catalytic mechanism for EH. The
mechanism of HLD, established by X-ray crystallographic analysis of an
HDL-substrate intermediate [4], involves nucleophilic attack of Asp-124 on
the halogen-substituted terminal carbon of the substrate, forming a
covalently-bound ester intermediate. The Asp-260/His-289 pair activate a
water molecule that hydrolyses the ester intermediate to release the
product. Mutagenesis experiments on murine soluble EH confirmed the crucial
role of nucleophile Asp-333 and His-523 in the catalytic mechanism and 
the importance of conserved His-263 and His-332 [5].
 
EPOXHYDRLASE is a 6-element fingerprint that provides a signature for the
epoxide hydrolase family. The fingerprint was derived from an initial 
alignment of 9 sequences: the motifs were drawn from conserved regions
within domain I - motif 1 contains highly conserved residues that are 
putatively involved in forming an oxyanion hole, which stabilises an 
enzyme-substrate tetrahedral intermediate [1,4]; motif 3 includes an
invariant Asn (Asp-124 in HDL); motifs 5 and 6 contain a conserved
acidic residue and an invariant His, respectively, corresponding to the
Asp-260/His-289 pair of HDL (motifs 2-5 correspond to ABHYDROLASE motifs
1-4). Two iterations on OWL26.0 were required to reach convergence, at
which point a true set comprising 16 sequences was identified. Several
partial matches were also found, most of which are hydrolases. The epoxide
hydrolase from Stigmatella aurantiaca (HYEP_STIAU), which shows only
marginal similarity to the EH family, matches only motifs 5 and 6.
 
An update on SPTR37_9f identified a true set of 22 sequences, and 133
partial matches.
Summary Information
  22 codes involving  6 elements
6 codes involving 5 elements
15 codes involving 4 elements
31 codes involving 3 elements
81 codes involving 2 elements
Composite Feature Index
6222222222222
5665355
41415134014
32226910422
2195611181246
123456
True Positives
HALO_XANAU    HYEP_HUMAN    HYEP_RABIT    HYEP_RAT      
HYES_HUMAN HYES_MOUSE HYES_RAT O06266
O48789 O49857 O81299 P79381
P97869 Q16764 Q25489 Q39856
Q41412 Q41413 Q41414 Q41415
Q42566 Q94806
True Positive Partials
Codes involving 5 elements
DEH1_MORSP O23445 O33550 O44124
P96811 Y0DA_MYCTU
Codes involving 4 elements
LINB_PSEPA LUCI_RENRE O06576 O23444
O31243 O31581 O52866 O66382
O69638 O86546 P73490 P73598
P95276 Q21277 Q23068
Codes involving 3 elements
BCHO_RHOCA BPA2_STRAU O05119 O05235
O05863 O06648 O18391 O23227
O53321 O82821 O82828 O82829
P71702 P73096 P73114 P73115
P73384 P73482 P91141 P95959
PRXC_PSEFL PRXC_STRAU PRXC_SYNY3 Q18638
Q50642 Q53042 Q54227 Q55390
Q59695 TPES_PSEPU YU14_MYCTU
Codes involving 2 elements
ACOC_ALCEU BPHD_BURCE DMPD_PSESP ESTE_PSEFL
LIP1_PSYIM LIP3_MORSP MHPC_ECOLI O05327
O06157 O06338 O06420 O06575
O06734 O07214 O14973 O15007
O22761 O22975 O22977 O27948
O28567 O28735 O29396 O31266
O31386 O32873 O32958 O33745
O33766 O34312 O41892 O51858
O52809 O54172 O60135 O66664
O67895 O67982 O69263 O69356
O69794 O73957 O85289 O85736
O85983 O86787 O86901 O87637
O88499 P91143 P93732 P96446
P96688 P96851 PHAB_PSEOL PIP_NEIGO
PLDB_HAEIN PRXC_PSEPY PRXC_STRLI Q07646
Q21147 Q45289 Q47858 Q49926
Q52011 Q52036 Q52532 Q52897
Q53540 Q59093 Q59248 Q59324
Q92571 RRPL_MUMPM THCF_RHOER TODF_PSEPU
XYLF_PSEPU Y00P_MYCTU Y0D9_MYCTU YHN5_YEAST
YN93_YEAST
Sequence Titles
HALO_XANAU  HALOALKANE DEHALOGENASE (EC 3.8.1.5) - XANTHOBACTER AUTOTROPHICUS. 
HYEP_HUMAN EPOXIDE HYDROLASE (EC 3.3.2.3) (MICROSOMAL EPOXIDE HYDROLASE) (EPOXIDE HYDRATASE) - HOMO SAPIENS (HUMAN).
HYEP_RABIT EPOXIDE HYDROLASE (EC 3.3.2.3) (MICROSOMAL EPOXIDE HYDROLASE) (EPOXIDE HYDRATASE) - ORYCTOLAGUS CUNICULUS (RABBIT).
HYEP_RAT EPOXIDE HYDROLASE (EC 3.3.2.3) (MICROSOMAL EPOXIDE HYDROLASE) (EPOXIDE HYDRATASE) - RATTUS NORVEGICUS (RAT).
HYES_HUMAN SOLUBLE EPOXIDE HYDROLASE (SEH) (EC 3.3.2.3) (EPOXIDE HYDRATASE) (CYTOSOLIC EPOXIDE HYDROLASE) (CEH) - HOMO SAPIENS (HUMAN).
HYES_MOUSE SOLUBLE EPOXIDE HYDROLASE (SEH) (EC 3.3.2.3) (EPOXIDE HYDRATASE) (CYTOSOLIC EPOXIDE HYDROLASE) (CEH) - MUS MUSCULUS (MOUSE).
HYES_RAT SOLUBLE EPOXIDE HYDROLASE (SEH) (EC 3.3.2.3) (EPOXIDE HYDRATASE) (CYTOSOLIC EPOXIDE HYDROLASE) (CEH) - RATTUS NORVEGICUS (RAT).
O06266 REGION B OF COSMID SCY07H7 - MYCOBACTERIUM TUBERCULOSIS.
O48789 PUTATIVE SOLUBLE EPOXIDE HYDROLASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O49857 ASPARAGINE-BOND SPECIFIC CYSTEINE ENDOPROTEASE - GLYCINE MAX (SOYBEAN).
O81299 T14P8.15 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
P79381 EPOXIDE HYDROLASE - SUS SCROFA (PIG).
P97869 MICROSOMAL EPOXIDE HYDROLASE (EC 3.3.2.3) (EPOXIDE HYDRATASE) (ARENE-OXIDE HYDRATASE) - MUS MUSCULUS (MOUSE).
Q16764 EPOXIDE HYDROLASE (EC 3.3.2.3) (EPOXIDE HYDRATASE) (ARENE-OXIDE HYDRATASE) - HOMO SAPIENS (HUMAN).
Q25489 JUVENILE HORMONE-SPECIFIC EPOXIDE HYDROLASE (EC 3.3.2.3) (MICROSOMAL EPOXIDE HYDROLASE) (EPOXIDE HYDRATASE) - MANDUCA SEXTA (TOBACCO HAWKMOTH) (TOBACCO HORNWORM).
Q39856 EPOXIDE HYDROLASE - GLYCINE MAX (SOYBEAN).
Q41412 EPOXIDE HYDROLASE - SOLANUM TUBEROSUM (POTATO).
Q41413 EPOXIDE HYDROLASE - SOLANUM TUBEROSUM (POTATO).
Q41414 EPOXIDE HYDROLASE - SOLANUM TUBEROSUM (POTATO).
Q41415 EPOXIDE HYDROLASE - SOLANUM TUBEROSUM (POTATO).
Q42566 ATSEH (EC 3.3.2.3) (EPOXIDE HYDROLASE) (EPOXIDE HYDRATASE) (ARENE-OXIDE HYDRATASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q94806 MICROSOMAL EPOXIDE HYDROLASE - TRICHOPLUSIA NI (CABBAGE LOOPER).

DEH1_MORSP HALOACETATE DEHALOGENASE H-1 (EC 3.8.1.3) - MORAXELLA SP.
O23445 SIMILARITY TO ATSEH - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O33550 BCHO PROTEIN - RHODOBACTER SPHAEROIDES (RHODOPSEUDOMONAS SPHAEROIDES).
O44124 EPOXIDE HYDROLASE - TRICHOPLUSIA NI (CABBAGE LOOPER).
P96811 HYPOTHETICAL 33.8 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
Y0DA_MYCTU HYPOTHETICAL 32.2 KD PROTEIN CY1A11.10 - MYCOBACTERIUM TUBERCULOSIS.

LINB_PSEPA 1,3,4,6-TETRACHLORO-1,4-CYCLOHEXADIENE HYDROLASE (EC 3.8.1.-) (1,4- TCDN CHLOROHYDROLASE) - PSEUDOMONAS PAUCIMOBILIS (SPHINGOMONAS PAUCIMOBILIS).
LUCI_RENRE RENILLA-LUCIFERIN 2-MONOOXYGENASE (EC 1.13.12.5) (RENILLA-TYPE LUCIFERASE) - RENILLA RENIFORMIS (SEA PANSY).
O06576 HYPOTHETICAL 34.7 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O23444 ATSEH HOMOLOG - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O31243 EPOXIDE HYDROLASE (EC 3.3.2.3) (EPOXIDE HYDRATASE) (ARENE-OXIDE HYDRATASE) - AGROBACTERIUM RADIOBACTER.
O31581 YFHM PROTEIN - BACILLUS SUBTILIS.
O52866 SOLUBLE EPOXIDE HYDROLASE (SEH) (EC 3.3.2.3) (EPOXIDE HYDRATASE) (CYTOSOLIC EPOXIDE HYDROLASE) (EH) - CORYNEBACTERIUM SP.
O66382 ESTERASE2 - ACETOBACTER PASTEURIANUS.
O69638 PUTATIVE EPOXIDE HYDROLASE - MYCOBACTERIUM TUBERCULOSIS.
O86546 PUTATIVE HYDROLASE - STREPTOMYCES COELICOLOR.
P73490 2-HYDROXY-6-OXOHEPTA-2,4-DIENOATE HYDROLASE - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P73598 HYPOTHETICAL 32.9 KD PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P95276 HYPOTHETICAL 39.3 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
Q21277 K07C5.5 PROTEIN - CAENORHABDITIS ELEGANS.
Q23068 SIMILAR TO EPOXIDE HYDROLASE - CAENORHABDITIS ELEGANS.

BCHO_RHOCA MAGNESIUM-CHELATASE 30 KD SUBUNIT - RHODOBACTER CAPSULATUS (RHODOPSEUDOMONAS CAPSULATA).
BPA2_STRAU NON-HAEM BROMOPEROXIDASE BPO-A2 (EC 1.11.1.-) (BROMIDE PEROXIDASE) (BPO2) - STREPTOMYCES AUREOFACIENS.
O05119 PUTATIVE GLYCERATE KINASE AND PYRUVATE KINASE (PYKA) GENES, COMPLETE CDS (PYKA) - METHYLOBACTERIUM EXTORQUENS.
O05235 HYPOTHETICAL 31.3 KD PROTEIN - BACILLUS SUBTILIS.
O05863 LIPV PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O06648 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) - PSEUDOMONAS AZELAICA.
O18391 KRAKEN GENE - DROSOPHILA MELANOGASTER (FRUIT FLY).
O23227 HYPOTHETICAL 41.9 KD PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O53321 HYPOTHETICAL 32.1 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O82821 2-HYDROXY-6-OXO-6-(2'-AMINOPHENYL)HEXA-2, 4-DIENOATE HYDROLASE - PSEUDOMONAS STUTZERI (PSEUDOMONAS PERFECTOMARINA).
O82828 ETBD1 - RHODOCOCCUS SP.
O82829 ETBD2 - RHODOCOCCUS SP.
P71702 HYPOTHETICAL 32.1 KD PROTEIN CY21D4.08C - MYCOBACTERIUM TUBERCULOSIS.
P73096 HYPOTHETICAL 31.9 KD PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P73114 ESTERASE - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P73115 HYPOTHETICAL 33.2 KD PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P73384 HYPOTHETICAL 29.7 KD PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P73482 HYPOTHETICAL 32.8 KD PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P91141 SIMILAR TO ACETYLTRANSFERASES - CAENORHABDITIS ELEGANS.
P95959 ORF C04020 - SULFOLOBUS SOLFATARICUS.
PRXC_PSEFL NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) (CPO-F) (CHLOROPEROXIDASE F) - PSEUDOMONAS FLUORESCENS.
PRXC_STRAU NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) (CPO-T) (CHLOROPEROXIDASE T) - STREPTOMYCES AUREOFACIENS.
PRXC_SYNY3 PUTATIVE NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
Q18638 C45B11.2 PROTEIN - CAENORHABDITIS ELEGANS.
Q50642 HYPOTHETICAL 33.7 KD PROTEIN CY227.22C - MYCOBACTERIUM TUBERCULOSIS.
Q53042 HALOALKANE DEHALOGENASE - RHODOCOCCUS RHODOCHROUS.
Q54227 MACROTETROLIDE ANTIBIOTIC-RESISTANCE PROTEIN - STREPTOMYCES GRISEUS.
Q55390 HYPOTHETICAL 38.3 KD PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
Q59695 DIHYDROLIPOAMIDE ACETYLTRANSFERASE (EC 2.3.1.12) (DIHYDROLIPOAMIDE S-ACETYLTRANSFERASE) (LIPOATE ACETYLTRANSFERASE) (THIOLTRANSACETYLASE A) - PSEUDOMONAS PUTIDA.
TPES_PSEPU TROPINESTERASE (EC 3.1.1.10) (ATROPINESTERASE) (ATROPINE ACYLHYDROLASE) - PSEUDOMONAS PUTIDA.
YU14_MYCTU PUTATIVE HALOALKANE DEHALOGENASE (EC 3.8.1.5) - MYCOBACTERIUM TUBERCULOSIS.

ACOC_ALCEU PROBABLE DIHYDROLIPOAMIDE ACETYLTRANSFERASE (EC 2.3.1.12) (FAST- MIGRATING PROTEIN) (FMP) - ALCALIGENES EUTROPHUS.
BPHD_BURCE 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) - BURKHOLDERIA CEPACIA (PSEUDOMONAS CEPACIA).
DMPD_PSESP 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE (EC 3.1.1.-) (HMSH) - PSEUDOMONAS SP. (STRAIN CF600).
ESTE_PSEFL ARYLESTERASE (EC 3.1.1.2) (ARYL-ESTER HYDROLASE) - PSEUDOMONAS FLUORESCENS.
LIP1_PSYIM LIPASE 1 PRECURSOR (EC 3.1.1.3) (TRIACYLGLYCEROL LIPASE) - PSYCHROBACTER IMMOBILIS.
LIP3_MORSP LIPASE 3 PRECURSOR (EC 3.1.1.3) (TRIACYLGLYCEROL LIPASE) - MORAXELLA SP.
MHPC_ECOLI 2-HYDROXY-6-KETONONA-2,4-DIENEDIOIC ACID HYDROLASE (EC 3.7.1.-) - ESCHERICHIA COLI.
O05327 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE - PSEUDOMONAS SP.
O06157 HYPOTHETICAL 28.5 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O06338 HYPOTHETICAL 28.2 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O06420 HYPOTHETICAL 28.3 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O06575 HYPOTHETICAL 32.5 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O06734 PUTATIVE - PROBABLE ESTERASE - BACILLUS SUBTILIS.
O07214 HYPOTHETICAL 36.9 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O14973 PEG1/MEST - HOMO SAPIENS (HUMAN).
O15007 PEG1/MEST GENE MRNA - HOMO SAPIENS (HUMAN).
O22761 HYPOTHETICAL 128.8 KD PROTEIN.
O22975 T19F06.4 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O22977 T19F06.6 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O27948 CARBOXYLESTERASE (EST-3) - ARCHAEOGLOBUS FULGIDUS.
O28567 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID HYDROLASE (PCBD) - ARCHAEOGLOBUS FULGIDUS.
O28735 CARBOXYLESTERASE (EST-2) - ARCHAEOGLOBUS FULGIDUS.
O29396 CARBOXYLESTERASE (EST-1) - ARCHAEOGLOBUS FULGIDUS.
O31266 1H-3-HYDROXY-4-OXOQUINALDINE 2,4-DIOXYGENASE - ARTHROBACTER SP.
O31386 B-KETOADIPATE ENOL-LACTONE HYDROLASE (EC 3.1.1.24) (3-OXOADIPATE ENOL-LACTONASE) (CARBOXYMETHYLBUTENOLIDE LACTONASE) - BRADYRHIZOBIUM JAPONICUM.
O32873 HYPOTHETICAL 31.8 KD PROTEIN - MYCOBACTERIUM LEPRAE.
O32958 OXIDOREDUCTASE - MYCOBACTERIUM LEPRAE.
O33745 THIOSTERASE - STREPTOMYCES SP.
O33766 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE - SPHINGOMONAS SP.
O34312 YTXM - BACILLUS SUBTILIS.
O41892 POLYPROTEIN PRECURSOR - HEPATITIS GB VIRUS A.
O51858 2-HYDROXY-6-OXO-7-METHYLOCTA-2,4-DIENOATE HYDROLASE - PSEUDOMONAS PUTIDA.
O52809 PCZA361.30 - AMYCOLATOPSIS ORIENTALIS.
O54172 HYDROLASE - STREPTOMYCES COELICOLOR.
O60135 FATTY ACID COA LIGASE - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O66664 HYPOTHETICAL 29.5 KD PROTEIN - AQUIFEX AEOLICUS.
O67895 HYPOTHETICAL 23.9 KD PROTEIN - AQUIFEX AEOLICUS.
O67982 3-OXOADIPATE ENOL-LACTONE HYDROLASE/4-CARBOXYMUCONOLACTONE DECARBOXYLASE (EC 3.1.1.24) (3-OXOADIPATE ENOL-LACTONASE) (CARBOXYMETHYLBUTENOLIDE LACTONASE) - RHODOCOCCUS OPACUS.
O69263 2-HYDROXY-6-OXO-2,4-HEPTADIENOATE HYDROLASE - BURKHOLDERIA SP.
O69356 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE - RHODOCOCCUS ERYTHROPOLIS.
O69794 2-HYDROXY-6-OXOHEPTA-2,4-DIENOATE HYDROLASE - RALSTONIA SP.
O73957 LIPOLYTIC ENZYME - SULFOLOBUS ACIDOCALDARIUS.
O85289 HYDROLASE - SPHINGOMONAS SP. CB3.
O85736 NOND - STREPTOMYCES GRISEUS SUBSP. GRISEUS.
O85983 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE - SPHINGOMONAS AROMATICIVORANS.
O86787 HYPOTHETICAL 44.0 KD PROTEIN - STREPTOMYCES COELICOLOR.
O86901 HYDROLASE, DXNB PROTEIN - SPHINGOMONAS SP.
O87637 LACTONE-SPECIFIC ESTERASE - PSEUDOMONAS FLUORESCENS.
O88499 SARCOSINE DEHYDROGENASE (EC 1.5.99.1) - RATTUS NORVEGICUS (RAT).
P91143 SIMILAR TO ACETYLTRANSFERASES - CAENORHABDITIS ELEGANS.
P93732 PROLINE IMINOPEPTIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
P96446 EXPA5 - SINORHIZOBIUM MELILOTI.
P96688 CARBOXYLESTERASE NP - BACILLUS SUBTILIS.
P96851 HYPOTHETICAL 31.9 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
PHAB_PSEOL POLY(3-HYDROXYALKANOATE) DEPOLYMERASE (EC 3.1.1.-) (PHA DEPOLYMERASE) (PHB DEPOLYMERASE) - PSEUDOMONAS OLEOVORANS.
PIP_NEIGO PROLINE IMINOPEPTIDASE (EC 3.4.11.5) (PROLYL AMINOPEPTIDASE) - NEISSERIA GONORRHOEAE.
PLDB_HAEIN PROBABLE LYSOPHOSPHOLIPASE L2 (EC 3.1.1.5) (LECITHINASE B) - HAEMOPHILUS INFLUENZAE.
PRXC_PSEPY NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) (CPO-P) (CHLOROPEROXIDASE P) - PSEUDOMONAS PYRROCINIA.
PRXC_STRLI NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) (CPO-L) (CHLOROPEROXIDASE L) - STREPTOMYCES LIVIDANS.
Q07646 PEG1/MEST PROTEIN - MUS MUSCULUS (MOUSE).
Q21147 SIMILAR TO EPOXIDE HYDROLASE - CAENORHABDITIS ELEGANS.
Q45289 GLUTARYL 7-ACA ACYLASE PRECURSOR - BREVIBACILLUS LATEROSPORUS.
Q47858 HRCO, HRCP, HRCQ, HRCR, HRCS, HRCT & HRCU GENES - ERWINIA HERBICOLA.
Q49926 TPEA - MYCOBACTERIUM LEPRAE.
Q52011 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID HYDROLASE - PSEUDOMONAS PSEUDOALCALIGENES.
Q52036 2-HYDROXY-6-PHENYLHEXA-2 - PSEUDOMONAS PUTIDA.
Q52532 PCBD - PSEUDOMONAS SP.
Q52897 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) (HOPD HYDROLASE) - RHODOCOCCUS SP.
Q53540 ESTERASE - PSEUDOMONAS PUTIDA.
Q59093 3-OXOADIPATE ENOL-LACTONASE I (EC 3.1.1.24) (ENOL-LACTONE HYDROLASE I) (BETA-KETOADIPATE ENOL-LACTONE HYDROLASE I) - ACINETOBACTER CALCOACETICUS.
Q59248 CARBOXYLESTERASE NP (EC 3.1.1.1) (ALI-ESTERASE) (B-ESTERASE) (MONOBUTYRASE) (COCAINE ESTERASE) (PROCAINE ESTERASE) (METHYLBUTYRASE) - BACILLUS SUBTILIS.
Q59324 BETA-D-GALACTOSIDASE (EC 3.2.1.23) (BETA-GALACTOSIDASE) (LACTASE) - COMAMONAS TESTOSTERONI (PSEUDOMONAS TESTOSTERONI).
Q92571 MEST PROTEIN - HOMO SAPIENS (HUMAN).
RRPL_MUMPM RNA POLYMERASE BETA SUBUNIT (EC 2.7.7.48) (LARGE STRUCTURAL PROTEIN) (L PROTEIN) - MUMPS VIRUS (STRAIN MIYAHARA VACCINE).
THCF_RHOER NON-HEME HALOPEROXIDASE (EC 1.11.1.-) - RHODOCOCCUS ERYTHROPOLIS.
TODF_PSEPU 2-HYDROXY-6-OXO-2,4-HEPTADIENOATE HYDROLASE (HOHH) (EC 3.1.1.-) - PSEUDOMONAS PUTIDA.
XYLF_PSEPU 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE (EC 3.1.1.-) (HMSH) - PSEUDOMONAS PUTIDA.
Y00P_MYCTU PROBABLE OXIDOREDUCTASE EPHD (EC 1.-.-.-) - MYCOBACTERIUM TUBERCULOSIS.
Y0D9_MYCTU HYPOTHETICAL 31.7 KD PROTEIN CY1A11.09C - MYCOBACTERIUM TUBERCULOSIS.
YHN5_YEAST HYPOTHETICAL 44.9 KD PROTEIN IN ERG7-NMD2 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
YN93_YEAST HYPOTHETICAL 32.8 KD PROTEIN IN BIO3-HXT17 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Scan History
OWL26_0    2  1000 NSINGLE    
SPTR37_9f 2 400 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
HGEPTWSYLYRKMIPVFAE HALO_XANAU 54 54 -
HGFPQNRAMWARVAPQLAE DEH1_MORSP 33 33 -
HGFPELWYSWRHQMVYLAE STU02494 31 31 -
HGFPESWFSWRYQIPALAQ HYES_RAT 263 263 -
HGFPESWFSWRYQIPALAQ HYES_MOUSE 262 262 -
HGWPGSFYEFYKIIPLLTD HYEP_HUMAN 148 148 -
HGWPGSFYEFYKIIPLLTD HYEP_RAT 148 148 -
HGFPESWYSWRYQIPALAQ HYES_HUMAN 264 264 -
HGWPGSFFEFYKIIPLLTD HYEP_RABIT 148 148 -

Motif 2 width=16
Element Seqn Id St Int Rpt
GYRVLAMDMKGYGESS HYES_HUMAN 284 1 -
GFRVLAIDMKGYGDSS HYES_RAT 283 1 -
GFRVLAIDMKGYGDSS HYES_MOUSE 282 1 -
HHTVVCADLRGYGDSD DEH1_MORSP 52 0 -
VFEVICPSIPGYGYSE HYEP_RAT 177 10 -
GYRAVAPDLRGYGDTT STU02494 51 1 -
VFEVICPSIPGYGFSE HYEP_HUMAN 177 10 -
GARVIAPDFFGFGKSD HALO_XANAU 74 1 -
IFEVICPSIPGYGFSQ HYEP_RABIT 177 10 -

Motif 3 width=14
Element Seqn Id St Int Rpt
FIGHDWAGVMVWNM HYES_MOUSE 328 30 -
VVAHDWGALIAWHL STU02494 101 34 -
LVGHDRGGRTGHRM DEH1_MORSP 101 33 -
FIGHDWAGVLVWNM HYES_RAT 329 30 -
IQGGDWGSLICTNM HYEP_HUMAN 222 29 -
IQGGDWGSLICTNM HYEP_RAT 222 29 -
FIGHDWGGMLVWYM HYES_HUMAN 330 30 -
IQGGDWGALVCTNM HYEP_RABIT 222 29 -
LVVQDWGGFLGLTL HALO_XANAU 120 30 -

Motif 4 width=14
Element Seqn Id St Int Rpt
AQMVPNHVKGLHLN HYEP_RAT 236 0 -
ALDHPEAVLSLTVM DEH1_MORSP 115 0 -
CLFRPDKVKALVNL STU02494 115 0 -
PMADPSRFKRLIIM HALO_XANAU 134 0 -
AQLVPSHVKGLHLN HYEP_RABIT 236 0 -
ALFYPERVRAVASL HYES_HUMAN 344 0 -
ALFHPERVRAVASL HYES_RAT 343 0 -
ALFYPERVRAVASL HYES_MOUSE 342 0 -
AQLVPSHVKGLHLN HYEP_HUMAN 236 0 -

Motif 5 width=17
Element Seqn Id St Int Rpt
VPALMVTAEKDIVLRPE HYES_RAT 485 128 -
VPALMVTAEKDIVLRPE HYES_MOUSE 484 128 -
VPTGFSAFPFELLHTPE HYEP_HUMAN 394 144 -
VPTGFSAFPSELLHAPE HYEP_RAT 394 144 -
IPALMVTAEKDFVLVPQ HYES_HUMAN 485 127 -
VPTGFAAFPCEIMHVPE HYEP_RABIT 394 144 -
GQTFMAIGMKDKLLGPD HALO_XANAU 250 102 -
VFYGSKGQMGQLFDIPA DEH1_MORSP 238 109 -
VPTKFIVGEFDLVYHIP STU02494 255 126 -

Motif 6 width=23
Element Seqn Id St Int Rpt
MVRGGHFAAFEEPELLAQDIRKF HYEP_HUMAN 426 15 -
IADAGHFVQEFGEQVAREALKHF HALO_XANAU 284 17 -
MPRGGHFAAFEEPELLARDICKF HYEP_RABIT 426 15 -
IEDCGHWTQMDKPTEVNQILIKW HYES_HUMAN 518 16 -
MERGGHFAAFEEPKLLAQDIRKF HYEP_RAT 426 15 -
IEDCGHWTQIEKPTEVNQILIKW HYES_MOUSE 517 16 -
IEDCGHWTQIEKPAEVNQILIKW HYES_RAT 518 16 -
SLPGGHFFVDQFPAETSEILLKF DEH1_MORSP 267 12 -
LEGSAHFVNQERPHEISKHIYDF STU02494 295 23 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
HGFPELWYSWRHQIPGLAA Q42566 30 30 -
HGFPELWYSWRHQISGLAA O48789 30 30 -
HGFPELWYSWRHQMVYLAE Q41412 31 31 -
HGFPELWYSWRHQMVYLAE Q41415 31 31 -
HGFPELWYSWRHQMVYLAE Q41413 31 31 -
HGFPELWYSWRHQMVYLAE Q41414 31 31 -
HGFPELWYSWRHQILSLSS O49857 56 56 -
HGFPELWYSWRHQILSLSS Q39856 56 56 -
HGFPDLWYSWRHQLVSFAA O81299 31 31 -
HGFPESWFSWRYQIPALAQ HYES_RAT 263 263 -
HGFPESWFSWRYQIPALAQ HYES_MOUSE 262 262 -
HGWPGSFYEFYKIIPLLTD HYEP_HUMAN 148 148 -
HGWPGSFYEFYKIIPLLTD HYEP_RAT 148 148 -
HGFPESWYSWRYQIPALAQ HYES_HUMAN 264 264 -
HGFPESWYSWRYQIPALAQ Q16764 265 265 -
HGWPGCFYEFYKIIPLLTD P79381 148 148 -
HGWPGSFYEFYKIIPLLTD P97869 148 148 -
HGFPELAYSWRHQIPALAD O06266 33 33 -
HGWPGSFFEFYKIIPLLTD HYEP_RABIT 148 148 -
HGWPGSVREFYESIPLLTA Q94806 151 151 -
HGWPSSSKEFDKVIPILTT Q25489 152 152 -
HGEPTWSYLYRKMIPVFAE HALO_XANAU 54 54 -

Motif 2 width=16
Element Seqn Id St Int Rpt
GYRAVAPDLRGYGDSD Q42566 50 1 -
GYRAVAPDLRGYGDSD O48789 50 1 -
GYRAVAPDLRGYGDTT Q41412 51 1 -
GYRAVAPDLRGYGDTT Q41415 51 1 -
GYRAVAPDLRGYGDTT Q41413 51 1 -
GYRAVAPVLRGYGDTT Q41414 51 1 -
GYRAVAPDLRGYGDTE O49857 76 1 -
GYRAVAPDLRGYGDTE Q39856 76 1 -
GYRAIAPDLRGYGDSD O81299 51 1 -
GFRVLAIDMKGYGDSS HYES_RAT 283 1 -
GFRVLAIDMKGYGDSS HYES_MOUSE 282 1 -
VFEVICPSIPGYGFSE HYEP_HUMAN 177 10 -
VFEVICPSIPGYGYSE HYEP_RAT 177 10 -
GYRVLAMDMKGYGESS HYES_HUMAN 284 1 -
GYRVLAMDMKGYGESS Q16764 285 1 -
VFEVICPSIPGYGFSE P79381 177 10 -
VFEVICPSIPGYGFSE P97869 177 10 -
GYHVLAPDQRGYGGSS O06266 53 1 -
IFEVICPSIPGYGFSQ HYEP_RABIT 177 10 -
ALEVIVPSLPGYGFSD Q94806 177 7 -
VFEVVAVDLPGYGFSE Q25489 178 7 -
GARVIAPDFFGFGKSD HALO_XANAU 74 1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
VVGHDWGALIAWYL Q42566 99 33 -
VVGHDWGALIAWYL O48789 98 32 -
VVAHDWGALIAWHL Q41412 101 34 -
VVAHDWGALIAWHL Q41415 101 34 -
VVAHDWGALIAWHL Q41413 101 34 -
VVAHDWGALIAWHL Q41414 101 34 -
LVAHDWGAIIGWYL O49857 122 30 -
LVAHDWGAIIGWYL Q39856 122 30 -
LVGHDWGAIVAWWL O81299 97 30 -
FIGHDWAGVLVWNM HYES_RAT 329 30 -
FIGHDWAGVMVWNM HYES_MOUSE 328 30 -
IQGGDWGSLICTNM HYEP_HUMAN 222 29 -
IQGGDWGSLICTNM HYEP_RAT 222 29 -
FIGHDWGGMLVWYM HYES_HUMAN 330 30 -
FIGHDWGGMLVWYM Q16764 331 30 -
LQGGDWGSLICTNM P79381 222 29 -
IQGGDWGSLICTNI P97869 222 29 -
WVGHDWGAVVVWNA O06266 99 30 -
IQGGDWGALVCTNM HYEP_RABIT 222 29 -
VQGGDWGSVIGTSL Q94806 222 29 -
IQAGDWGSQCATHM Q25489 223 29 -
LVVQDWGGFLGLTL HALO_XANAU 120 30 -

Motif 4 width=14
Element Seqn Id St Int Rpt
CLFRPDRVKALVNL Q42566 113 0 -
CLFRPDKVKALVNL O48789 112 0 -
CLFRPDKVKALVNL Q41412 115 0 -
CLFRPDKVKALVNL Q41415 115 0 -
CLFRPDKVKALVNL Q41413 115 0 -
CLFRPDKVKALVNS Q41414 115 0 -
CMFRPDKVKAYVCL O49857 136 0 -
CMFRPDKVKAYVCL Q39856 136 0 -
CMIRPDRVNALVNT O81299 111 0 -
ALFHPERVRAVASL HYES_RAT 343 0 -
ALFYPERVRAVASL HYES_MOUSE 342 0 -
AQLVPSHVKGLHLN HYEP_HUMAN 236 0 -
AQMVPNHVKGLHLN HYEP_RAT 236 0 -
ALFYPERVRAVASL HYES_HUMAN 344 0 -
ALFYPERVRAVASL Q16764 345 0 -
AQLVPSHVKGLHLN P79381 236 0 -
AQMVPNHVKGLHLN P97869 236 0 -
PLLHADRVAAVAAL O06266 113 0 -
AQLVPSHVKGLHLN HYEP_RABIT 236 0 -
ATFFPEEVLGYHAN Q94806 236 0 -
ATLFPDQVLGLHTN Q25489 237 0 -
PMADPSRFKRLIIM HALO_XANAU 134 0 -

Motif 5 width=17
Element Seqn Id St Int Rpt
VPTKFVIGELDLVYYMP Q42566 255 128 -
VPTKFVIGELDLVYYMP O48789 254 128 -
VPTKFIVGEFDLVYHIP Q41412 255 126 -
VPTKFIVGEFDLVYHIP Q41415 255 126 -
VPTKFIVGEFDLAYHMR Q41413 255 126 -
VPTKFIVGEFDLVYHIP Q41414 255 126 -
VPVKYITGELDMVYNSL O49857 275 125 -
VPVKYITGELDMVYNSL Q39856 275 125 -
VPVKFIVGDLDITYNIP O81299 250 125 -
VPALMVTAEKDIVLRPE HYES_RAT 485 128 -
VPALMVTAEKDIVLRPE HYES_MOUSE 484 128 -
VPTGFSAFPFELLHTPE HYEP_HUMAN 394 144 -
VPTGFSAFPSELLHAPE HYEP_RAT 394 144 -
IPALMVTAEKDFVLVPQ HYES_HUMAN 485 127 -
IPALMVTAEKDFVLVPQ Q16764 486 127 -
VPTGFAAFPSEVLHCPE P79381 393 143 -
VPTGYSAFPSEILHAPE P97869 394 144 -
VPSLFIAGTADPVLTFT O06266 259 132 -
VPTGFAAFPCEIMHVPE HYEP_RABIT 394 144 -
VPSWFIQGKYEIAYQPP Q94806 393 143 -
VPTAGINFKYEVLYQPD Q25489 391 140 -
GQTFMAIGMKDKLLGPD HALO_XANAU 250 102 -

Motif 6 width=23
Element Seqn Id St Int Rpt
MEGVAHFINQEKPQEILQIILDF Q42566 295 23 -
MEGVAHFLNQEKPQEILQIILDF O48789 294 23 -
LEGSAHFVNQERPHEISKHIYDF Q41412 295 23 -
LEGAAHFVSQERPHEISKHIYDF Q41415 295 23 -
LEGAAHFVNQERPHEISKHIYDF Q41413 295 23 -
LEGAAHFVNQERPHEISKHIYDF Q41414 295 23 -
QKGVAHFNNQEAAEEIDNYIYDF O49857 315 23 -
QKGVAHFNNQEAAEEIDNYIYDF Q39856 315 23 -
MEGVGHFLHQEKPDEVTDHIYGF O81299 290 23 -
IEDCGHWTQIEKPAEVNQILIKW HYES_RAT 518 16 -
IEDCGHWTQIEKPTEVNQILIKW HYES_MOUSE 517 16 -
MVRGGHFAAFEEPELLAQDIRKF HYEP_HUMAN 426 15 -
MERGGHFAAFEEPKLLAQDIRKF HYEP_RAT 426 15 -
IEDCGHWTQMDKPTEVNQILIKW HYES_HUMAN 518 16 -
IEDCGHWTQMDKPTEVNQILIKW Q16764 519 16 -
MARGGHFAAFEEPELLAQDIRKF P79381 425 15 -
MERGGHFAAFEEPKLLAQDIRKF P97869 426 15 -
IDGAGHWLQQERPGEVTAALLEF O06266 293 17 -
MPRGGHFAAFEEPELLARDICKF HYEP_RABIT 426 15 -
LDDGGHFFAFELPEVFSKDVLKA Q94806 425 15 -
LDFGGHFAALHTPQALADDIFAS Q25489 423 15 -
IADAGHFVQEFGEQVAREALKHF HALO_XANAU 284 17 -