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PR00410

Identifier
PHEHYDRXLASE  [View Relations]  [View Alignment]  
Accession
PR00410
No. of Motifs
6
Creation Date
30-OCT-1995  (UPDATE 30-JUN-1999)
Title
Phenol hydroxylase reductase family signature
Database References

INTERPRO; IPR001221
UMBBD; e0199; e0117; e0172
PDB; 1FNR
SCOP; 1FNR
CATH; 1FNR
Literature References
1. HYDE, G.E., CRAWFORD, N.M. AND CAMPBELL, W.H.
The sequence of squash NADH:nitrate reductase and its relationship to the
sequences of other flavoprotein oxidoreductases. A family of flavoprotein
pyridine nucleotide cytochrome reductases.
J.BIOL.CHEM. 266 23542-23547 (1991).
 
2. HARAYAMA, S., KOK, M. AND NEIDLE, E.L.
Functional and evolutionary relationships among diverse oxygenases.
ANNU.REV.MICROBIOL. 46 565-601 (1992).
 
3. NORDLUND I., POWLOWSKI J. AND SHINGLER V.
Complete nucleotide sequence and polypeptide analysis of multicomponent 
phenol hydroxylase from Pseudomonas sp. strain CF600.
J.BACTERIOL. 172 6826-6833 (1990).
 
4. NEIDLE, E.L., HARTNETT, C., ORNSTON, N.L., BAIROCH, A., REKIK, M. AND 
HARAYAMA, S.
Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for 
benzoate 1,2-dioxygenase reveal evolutionary relationships among 
multicomponent oxygenases.
J.BACTERIOL. 173 5385-5395 (1991).
 
5. HARAYAMA, S., REKIK, M., BAIROCH, A., NEIDLE, E.L. AND ORNSTON, L.N.
Potential DNA slippage structures acquired during evolutionary divergence 
of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida 
TOL pWW0 plasmid xylXYZ, genes encoding benzoate dioxygenases.
J.BACTERIOL. 173 7540-7548 (1991).
 
6. SUZUKI, M., HAYAKAWA, T., SHAW, J.P., REKIK, M. AND HARAYAMA, S.
Primary structure of xylene monooxygenase: similarities to and differences
from the alkane hydroxylation system.
J.BACTERIOL. 173 1690-1695 (1991).
 
7. STAINTHORPE, A.C., LEES, V., SALMOND, G.P., DALTON, H. AND MURRELL, J.C.
The methane monooxygenase gene cluster of Methylococcus capsulatus (Bath).
GENE 91 27-34 (1990).
 
8. KESSLER, A.C., HAASE, A. AND REEVES, P.R.
Molecular analysis of the 3,6-dideoxyhexose pathway genes of Yersinia 
pseudotuberculosis serogroup IIA.
J.BACTERIOL. 175 1412-1422 (1993).
 
9. JIANG, X.-M., NEAL, B., SANTIAGO, F., LEE, S.J., ROMANA, L.K. AND 
REEVES, P.R.
Structure and sequence of the rfb (O antigen) gene cluster of Salmonella 
serovar typhimurium (strain LT2).
MOL.MICROBIOL. 5 695-713 (1991).
 
10. CORRELL, C.C., LUDWIG, M.L., BRUNS, C.M. AND KARPLUS, P.A.
Structural prototypes for an extended family of flavoprotein reductases:
comparison of phthalate dioxygenase reductase with ferredoxin reductase
and ferredoxin.
PROTEIN SCI. 2 2112-2133 (1993).

Documentation
Flavoprotein pyridine nucleotide cytochrome reductases [1] (FPNCR) catalyse
the interchange of reducing equivalents between one-electron carriers and
the two-electron-carrying nicotinamide dinucleotides. The enzymes include
ferredoxin:NADP+ reductases (FNR); plant and fungal NAD(P)H:nitrate
reductases; NADH:cytochrome b5 reductases; NADPH:P450 reductases;
NADPH:sulfite reductases; nitric oxide synthases; phthalate dioxygenase 
reductase; and various other flavoproteins.
 
Dioxygen can be incorporated directly into organic compounds in reactions
catalysed by enzymes termed oxygenases or hydroxylases: oxygenases that
catalyse the incorporation of both atoms of dioxygen into substrates are
known as dioxygenases; those that catalyse the incorporation of only one
atom of dioxygen are termed monooxygenases, or mixed-function oxygenases.
The second atom of dioxygen is reduced to H2O either by the substrates
themselves or by a co-substrate reductant [2].
 
Electron transfer components of a number of bacterial multi-component 
aromatic-ring and alkyl-group oxygenases (including phenol hydroxylase [3],
benzoate 1,2-dioxygenase [4], toluate 1,2-dioxygenase [5], xylene 
monooxygenase [6] and methane monooxygenase [7]), belong to family of 
[2Fe-2S] ferredoxin/FPNCR fusion proteins. Other members of the family are 
Yersinia pseudotuberculosis CDP-6-deoxy-3,4-glucoseen reductase [8] and 
S.typhimurium rfbI protein [9]. All contain a [2Fe-2S] ferredoxin domain
fused to the N-terminus of an FPNCR domain. The direction of electron flow
is from flavin to the [2Fe-2S] center. 
 
PHEHYDRXLASE is a 6-element fingerprint that provides a signature for the
phenol hydroxylase family. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn solely from the FPNCR
domain - motifs 1-3 span the flavin-binding domain, and motifs 4-6 encode
the NAD(H)-binding domain. Motifs 1, 2, 4, 5 and 6 correspond to FPNCR
motifs 1, 2, 4, 5 and 8, respectively. Motif 2 appears to be involved in
binding the isoalloxazine ring of FAD; motif 4 includes residues involved
in binding the diphosphate group of the pyridine nucleotide; and motif 6
contains a conserved Cys residue that may interact with NAD(H) and is
necessary for efficient electron transfer [10]. Two iterations on OWL26.2
were required to reach convergence, at which point a true set comprising 11
sequences was identified. Several partial matches were also found:
reductase components of Pseudomonas putida naphthalene dioxygenase (JN0640,
JN0642) and polycyclic aromatic hydrocarbon dioxygenase (A55217) lack motif
2; and Pseudomonas pickettii toluene-3-monooxygenase reductase (PPU04052)
and Vibrio alginolyticus Na+-translocating NADH-quinone reductase 
(VIBNQR363) lack motif 5. Surprisingly, the mmoC component of Methylosinus 
trichosporium methane monooxygenase (C48360), which is functionally 
identical to the mmoC protein of Methylococcus capsulatus (MEMC_METCA), 
lacks motifs 5 and 6 (motif 6 is well conserved among most FPNCRs).
Pseudomonas cepacia 2-halobenzoate 1,2-dioxygenase reductase (PCCBDABC3)
lacks motifs 2 and 5; Pseudomonas mendocina toluene-4-monooxygenase
component tmoF (A47016) and Pseudomonas pseudoalcaligenes phenoxybenzoate
dioxygenase pobB (S44172) lack motifs 3 and 5. All these proteins are
[2Fe-2S] ferredoxin/FPNCR fusion enzymes, except S44172, which has a
[2Fe-2S] ferredoxin domain at its C-terminus. 
 
An update on SPTR37_9f identified a true set of 19 sequences, and 146
partial matches.
Summary Information
  19 codes involving  6 elements
14 codes involving 5 elements
11 codes involving 4 elements
16 codes involving 3 elements
84 codes involving 2 elements
Composite Feature Index
6191919191919
51491314812
410361159
3141001437
21430281635
123456
True Positives
ASCD_YERPS    BENC_ACICA    DMPP_PSESP    O30866        
O31003 O32433 O33457 O52644
O84963 O85971 P95461 Q17574
Q43983 Q52166 Q52168 Q52175
Q53028 RFBI_SALTY XYLA_PSEPU
True Positive Partials
Codes involving 5 elements
MEMC_METCA NDOR_PSEPU O05933 O52378
O69183 O85675 O87803 P95561
Q51492 Q51944 Q52140 Q53563
Q56584 XYLZ_PSEPU
Codes involving 4 elements
HMPA_ALCEU LUXG_PHOLE O05012 O85219
O87617 P95277 P96096 Q45692
Q51603 Q52574 TMOF_PSEME
Codes involving 3 elements
FENR_CYAPA FENR_SPISP FENR_SYNP2
FENR_SYNY3 LUXG_VIBHA O05875 O32476
O82824 O84745 O86363 P95533
POBB_PSEPS Q44253 Q47914 UBIB_VIBHA
Codes involving 2 elements
CADF_HUMAN CYSJ_ECOLI CYSJ_SALTY
FENR_ANASO FENR_ANAVA FENR_AZOVI FENR_CHLRE
FENR_ORYSA FENR_PEA FENR_SPIOL FENR_VICFA
FENS_ORYSA FENS_PEA FENT_ORYSA FHP_CANNO
HMPA_ERWCH HMPA_VIBPA LUXG_VIBFI MT10_YEAST
NC5R_BOVIN NC5R_HUMAN NC5R_RAT NCPR_CAVPO
NCPR_HUMAN NCPR_PHAAU NCPR_PIG NCPR_RABIT
NCPR_RAT NIA1_ARATH NIA1_BRANA NIA1_HORVU
NIA1_ORYSA NIA1_PHAVU NIA1_SOYBN NIA1_TOBAC
NIA2_ARATH NIA2_BRANA NIA2_PHAVU NIA2_SOYBN
NIA2_TOBAC NIA3_MAIZE NIA_CICIN NIA_CUCMA
NIA_LOTJA NIA_LYCES NIA_PETHY NIA_SPIOL
NIA_USTMA O04926 O16521 O16522
O24390 O24425 O29722 O32214
O59014 O68591 O74183 O84442
O84985 O86347 P75824 P85B_RAT
P94680 PDR_BURCE Q09590 Q12683
Q12684 Q26240 Q41641 Q42497
Q43042 Q43235 Q58841 Q59668
Q60451 Q92237 UBIB_ECOLI UBIB_VIBFI
VANB_ACICA VANB_PSEPU VANB_PSES9 VANB_PSESP
Sequence Titles
ASCD_YERPS  CDP-6-DEOXY-DELTA-3,4-GLUCOSEEN REDUCTASE (EC 1.17.1.-) (E3) - YERSINIA PSEUDOTUBERCULOSIS. 
BENC_ACICA BENZOATE 1,2-DIOXYGENASE ELECTRON TRANSFER COMPONENT [INCLUDES: FERREDOXIN; FERREDOXIN--NAD(+) REDUCTASE (EC 1.18.1.3)] - ACINETOBACTER CALCOACETICUS.
DMPP_PSESP PHENOL HYDROXYLASE P5 PROTEIN (EC 1.14.13.7) (PHENOL 2-MONOOXYGENASE P5 COMPONENT) - PSEUDOMONAS SP. (STRAIN CF600).
O30866 XYLENE MONOOXYGENASE ELECTRON TRANSFER COMPONENT - PSEUDOMONAS PUTIDA.
O31003 DDHD - VIBRIO ANGUILLARUM.
O32433 DMS OXYGENASE COMPONENT - ACINETOBACTER SP.
O33457 P-CYMENE MONOOXYGENASE REDUCTASE SUBUNIT - PSEUDOMONAS PUTIDA.
O52644 REDUCTASE COMPONENT OF NITROTOLUENE MONOXYGENASE NTNMA - PSEUDOMONAS SP.
O84963 PHENOL HYDROXYLASE COMPONENT - RALSTONIA SP. E2.
O85971 XYLENE MONOOXYGENASE ELECTRON TRANSFER SUBUNIT - SPHINGOMONAS AROMATICIVORANS.
P95461 P-CYMENE METHYL HYDROXYLASE - PSEUDOMONAS AUREOFACIENS.
Q17574 C01G6.6 PROTEIN - CAENORHABDITIS ELEGANS.
Q43983 PHENOLHYDROXYLASE COMPONENT - ACINETOBACTER CALCOACETICUS.
Q52166 PHENOL HYDROXYLASE P5 PROTEIN (EC 1.14.13.7) (PHENOL 2-MONOOXYGENASE P5 COMPONENT) - PSEUDOMONAS PUTIDA.
Q52168 ONE COMPONENT OF PHENOL HYDROXYLASE - PSEUDOMONAS PUTIDA.
Q52175 PHENOL HYDROXYLASE P5 PROTEIN (EC 1.14.13.7) (PHENOL 2-MONOOXYGENASE P5 COMPONENT) - PSEUDOMONAS PUTIDA.
Q53028 REDUCTASE - NOCARDIA CORALLINA.
RFBI_SALTY RFBI PROTEIN - SALMONELLA TYPHIMURIUM.
XYLA_PSEPU XYLENE MONOOXYGENASE ELECTRON TRANSFER COMPONENT [INCLUDES: FERREDOXIN; FERREDOXIN--NAD(+) REDUCTASE (EC 1.18.1.3)] - PSEUDOMONAS PUTIDA.

MEMC_METCA METHANE MONOOXYGENASE COMPONENT C (EC 1.14.13.25) (METHANE HYDROXYLASE) - METHYLOCOCCUS CAPSULATUS.
NDOR_PSEPU NAPHTHALENE 1,2-DIOXYGENASE SYSTEM FERREDOXIN--NAD(+) REDUCTASE COMPONENT (EC 1.18.1.3) - PSEUDOMONAS PUTIDA.
O05933 2-OXO-1,2-DIHYDROQUINOLINE 8-MONOOXYGENASE, REDUCTASE COMPONENT - PSEUDOMONAS PUTIDA.
O52378 FERREDOXIN REDUCTASE - PSEUDOMONAS SP.
O69183 PUTATIVE ELECTRON TRANSFER COMPONENT - ALCALIGENES EUTROPHUS.
O85675 ANTHRANILATE DIOXYGENASE REDUCTASE - ACINETOBACTER SP. ADP1.
O87803 OXIDOREDUCTASE - PSEUDOMONAS STUTZERI (PSEUDOMONAS PERFECTOMARINA).
P95561 REDUCTASE 2NT - PSEUDOMONAS SP.
Q51492 REDUCTASE - PSEUDOMONAS AERUGINOSA.
Q51944 TOLUENE-3-MONOOXYGENASE OXIDOREDUCTASE - BURKHOLDERIA PICKETTII (PSEUDOMONAS PICKETTII).
Q52140 FERREDOXIN REDUCTASE (EC 1.-.-.-) - PSEUDOMONAS PUTIDA.
Q53563 PROTEIN C - METHYLOSINUS TRICHOSPORIUM.
Q56584 NADH-UNIQUINONE OXIDOREDUCTASE SUBUNIT F (EC 1.6.5.3) (BETA SUBUNIT) - VIBRIO ALGINOLYTICUS.
XYLZ_PSEPU TOLUATE 1,2-DIOXYGENASE ELECTRON TRANSFER COMPONENT [INCLUDES: FERREDOXIN; FERREDOXIN--NAD(+) REDUCTASE (EC 1.18.1.3)] - PSEUDOMONAS PUTIDA.

HMPA_ALCEU FLAVOHEMOPROTEIN (HEMOGLOBIN-LIKE PROTEIN) (FLAVOHEMOGLOBIN) - ALCALIGENES EUTROPHUS.
LUXG_PHOLE PROBABLE FLAVIN REDUCTASE (EC 1.-.-.-) - PHOTOBACTERIUM LEIOGNATHI.
O05012 PHENOLHYDROXYLASE COMPONENT - HAEMOPHILUS INFLUENZAE.
O85219 PROBABLE FLAVIN REDUCTASE - PHOTOBACTERIUM LEIOGNATHI.
O87617 O-HALOBENZOATE DIOXYGENASE REDUCTASE - PSEUDOMONAS AERUGINOSA.
P95277 HYPOTHETICAL 93.4 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
P96096 NADH REDUCTASE - THIOBACILLUS FERROOXIDANS.
Q45692 FERREDOXIN OXIDOREDUCTASE - BURKHOLDERIA SP.
Q51603 2-HALOBENZOATE 1,2-DIOXYGENASE - BURKHOLDERIA CEPACIA (PSEUDOMONAS CEPACIA).
Q52574 OXIDOREDUCTASE - PSEUDOMONAS SP.
TMOF_PSEME TOLUENE-4-MONOOXYGENASE ELECTRON TRANSFER COMPONENT [INCLUDES: FERREDOXIN; FERREDOXIN--NAD(+) REDUCTASE (EC 1.18.1.3)] - PSEUDOMONAS MENDOCINA.


FENR_CYAPA FERREDOXIN--NADP REDUCTASE PRECURSOR (EC 1.18.1.2) (FNR) - CYANOPHORA PARADOXA.
FENR_SPISP FERREDOXIN--NADP REDUCTASE (EC 1.18.1.2) (FNR) - SPIRULINA SP.
FENR_SYNP2 FERREDOXIN--NADP REDUCTASE (EC 1.18.1.2) (FNR) - SYNECHOCOCCUS SP. (STRAIN PCC 7002) (AGMENELLUM QUADRUPLICATUM).
FENR_SYNY3 FERREDOXIN--NADP REDUCTASE (EC 1.18.1.2) (FNR) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
LUXG_VIBHA PROBABLE FLAVIN REDUCTASE (EC 1.-.-.-) - VIBRIO HARVEYI.
O05875 HYPOTHETICAL 40.8 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O32476 FERREDOXIN REDUCTASE COMPONENT OF CARBAZOLE 1,9A- DIOXYGENASE - PSEUDOMONAS SP.
O82824 FERREDOXIN REDUCTASE - PSEUDOMONAS STUTZERI (PSEUDOMONAS PERFECTOMARINA).
O84745 PHENOLHYDROLASE/NADH UBIQUINONE OXIDOREDUCTASE - CHLAMYDIA TRACHOMATIS.
O86363 HYPOTHETICAL 43.3 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
P95533 ELECTRON TRANSFER PROTEIN - PSEUDOMONAS PUTIDA.
POBB_PSEPS PHENOXYBENZOATE DIOXYGENASE BETA SUBUNIT (EC 1.-.-.-) (4-CARBOXYDIPHENYL ETHER;PHENOXYBENZOATE DIOXYGENASE BETA SUBUNIT) - PSEUDOMONAS PSEUDOALCALIGENES.
Q44253 ANILINE DIOXYGENASE REDUCTASE COMPONENT - ACINETOBACTER SP.
Q47914 PENTACHLOROPHENOL 4-MONOOXYGENASE REDUCTASE - FLAVOBACTERIUM SP.
UBIB_VIBHA NAD(P)H-FLAVIN REDUCTASE (EC 1.6.8.-) - VIBRIO HARVEYI.


CADF_HUMAN MUSCLE-CADHERIN PRECURSOR (M-CADHERIN) (CADHERIN-15) (CADHERIN-14) - Homo sapiens (Human).
CYSJ_ECOLI SULFITE REDUCTASE [NADPH] FLAVOPROTEIN ALPHA-COMPONENT (EC 1.8.1.2) (SIR-FP) - ESCHERICHIA COLI.
CYSJ_SALTY SULFITE REDUCTASE [NADPH] FLAVOPROTEIN ALPHA-COMPONENT (EC 1.8.1.2) (SIR-FP) - SALMONELLA TYPHIMURIUM.
FENR_ANASO FERREDOXIN--NADP REDUCTASE (EC 1.18.1.2) (FNR) - ANABAENA SP. (STRAIN PCC 7119).
FENR_ANAVA FERREDOXIN--NADP REDUCTASE (EC 1.18.1.2) (FNR) - ANABAENA VARIABILIS.
FENR_AZOVI FERREDOXIN--NADP REDUCTASE (EC 1.18.1.2) (FNR) (PROTEIN X) - AZOTOBACTER VINELANDII.
FENR_CHLRE FERREDOXIN--NADP REDUCTASE PRECURSOR (EC 1.18.1.2) (FNR) - CHLAMYDOMONAS REINHARDTII.
FENR_ORYSA FERREDOXIN--NADP REDUCTASE, LEAF ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - ORYZA SATIVA (RICE).
FENR_PEA FERREDOXIN--NADP REDUCTASE, LEAF ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - PISUM SATIVUM (GARDEN PEA).
FENR_SPIOL FERREDOXIN--NADP REDUCTASE PRECURSOR (EC 1.18.1.2) (FNR) - SPINACIA OLERACEA (SPINACH).
FENR_VICFA FERREDOXIN--NADP REDUCTASE PRECURSOR (EC 1.18.1.2) (FNR) - VICIA FABA (BROAD BEAN).
FENS_ORYSA FERREDOXIN--NADP REDUCTASE, ROOT ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - ORYZA SATIVA (RICE).
FENS_PEA FERREDOXIN--NADP REDUCTASE, ROOT ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - PISUM SATIVUM (GARDEN PEA).
FENT_ORYSA FERREDOXIN--NADP REDUCTASE, EMBRYO ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - ORYZA SATIVA (RICE).
FHP_CANNO FLAVOHEMOPROTEIN (HEMOGLOBIN-LIKE PROTEIN) (FLAVOHEMOGLOBIN) (DIHYDROPTERIDINE REDUCTASE (EC 1.6.99.7)) - CANDIDA NORVEGENSIS (YEAST) (CANDIDA MYCODERMA).
HMPA_ERWCH FLAVOHEMOPROTEIN (HEMOGLOBIN-LIKE PROTEIN) (FLAVOHEMOGLOBIN) (HEMOPROTEIN X) - ERWINIA CHRYSANTHEMI.
HMPA_VIBPA FLAVOHEMOPROTEIN (HEMOGLOBIN-LIKE PROTEIN) (FLAVOHEMOGLOBIN) - VIBRIO PARAHAEMOLYTICUS.
LUXG_VIBFI PROBABLE FLAVIN REDUCTASE (EC 1.-.-.-) - VIBRIO FISCHERI.
MT10_YEAST SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT (EC 1.8.1.2) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
NC5R_BOVIN NADH-CYTOCHROME B5 REDUCTASE (EC 1.6.2.2) - BOS TAURUS (BOVINE).
NC5R_HUMAN NADH-CYTOCHROME B5 REDUCTASE (EC 1.6.2.2) (B5R) - HOMO SAPIENS (HUMAN).
NC5R_RAT NADH-CYTOCHROME B5 REDUCTASE (EC 1.6.2.2) - RATTUS NORVEGICUS (RAT).
NCPR_CAVPO NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - CAVIA PORCELLUS (GUINEA PIG).
NCPR_HUMAN NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - HOMO SAPIENS (HUMAN).
NCPR_PHAAU NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - PHASEOLUS AUREUS (MUNG BEAN) (VIGNA RADIATA).
NCPR_PIG NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - SUS SCROFA (PIG).
NCPR_RABIT NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - ORYCTOLAGUS CUNICULUS (RABBIT).
NCPR_RAT NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - RATTUS NORVEGICUS (RAT).
NIA1_ARATH NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR1) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
NIA1_BRANA NITRATE REDUCTASE, CLONE PBNBR1405 (EC 1.6.6.1) (NR) - BRASSICA NAPUS (RAPE).
NIA1_HORVU NITRATE REDUCTASE (EC 1.6.6.1) (NR) - HORDEUM VULGARE (BARLEY).
NIA1_ORYSA NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR1) - ORYZA SATIVA (RICE).
NIA1_PHAVU NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR-1) - PHASEOLUS VULGARIS (KIDNEY BEAN) (FRENCH BEAN).
NIA1_SOYBN INDUCIBLE NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR) - GLYCINE MAX (SOYBEAN).
NIA1_TOBAC NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR1) - NICOTIANA TABACUM (COMMON TOBACCO).
NIA2_ARATH NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR2) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
NIA2_BRANA NITRATE REDUCTASE, CLONE PBNBR1412 (EC 1.6.6.1) (NR) - BRASSICA NAPUS (RAPE).
NIA2_PHAVU NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR-2) - PHASEOLUS VULGARIS (KIDNEY BEAN) (FRENCH BEAN).
NIA2_SOYBN INDUCIBLE NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR) - GLYCINE MAX (SOYBEAN).
NIA2_TOBAC NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR2) - NICOTIANA TABACUM (COMMON TOBACCO).
NIA3_MAIZE NITRATE REDUCTASE 3 (EC 1.6.6.1) (NR) - ZEA MAYS (MAIZE).
NIA_CICIN NITRATE REDUCTASE (EC 1.6.6.1) (NR) - CICHORIUM INTYBUS (CHICORY).
NIA_CUCMA NITRATE REDUCTASE (EC 1.6.6.1) (NR) - CUCURBITA MAXIMA (PUMPKIN) (WINTER SQUASH).
NIA_LOTJA NITRATE REDUCTASE (EC 1.6.6.1) (NR) - LOTUS JAPONICUS.
NIA_LYCES NITRATE REDUCTASE (EC 1.6.6.1) (NR) - LYCOPERSICON ESCULENTUM (TOMATO).
NIA_PETHY NITRATE REDUCTASE (EC 1.6.6.1) (NR) - PETUNIA HYBRIDA (PETUNIA).
NIA_SPIOL NITRATE REDUCTASE (EC 1.6.6.1) (NR) - SPINACIA OLERACEA (SPINACH).
NIA_USTMA NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - USTILAGO MAYDIS (SMUT FUNGUS).
O04926 NADH NITRATE REDUCTASE (EC 1.6.6.3) (NITRATE REDUCTASE (NADPH)) - SOLANUM TUBEROSUM (POTATO).
O16521 T05H4.5 PROTEIN - CAENORHABDITIS ELEGANS.
O16522 T05H4.4 PROTEIN - CAENORHABDITIS ELEGANS.
O24390 NADH NITRATE REDUCTASE (EC 1.6.6.3) - SOLANUM TUBEROSUM (POTATO).
O24425 NADPH:FERRIHEMOPROTEIN OXIDOREDUCTASE (EC 1.6.2.4) - ESCHSCHOLZIA CALIFORNICA (CALIFORNIA POPPY).
O29722 CYTOCHROME-C3 HYDROGENASE, SUBUNIT GAMMA - ARCHAEOGLOBUS FULGIDUS.
O32214 YVGR PROTEIN - BACILLUS SUBTILIS.
O59014 292AA LONG HYPOTHETICAL CYTOCHROME-C3 HYDROGENASE GAMMA CHAIN - PYROCOCCUS HORIKOSHII.
O68591 IRON-UPTAKE FACTOR - PSEUDOMONAS AERUGINOSA.
O74183 FLAVOHEMOGLOBIN - FUSARIUM OXYSPORUM.
O84442 SULFITE REDUCTASE - CHLAMYDIA TRACHOMATIS.
O84985 PHAI - PSEUDOMONAS PUTIDA.
O86347 HYPOTHETICAL 33.5 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
P75824 FROM BASES 910309 TO 921580 (SECTION 79 OF 400) OF THE COMPLETE GENOME (SECTION 79 OF 400) - ESCHERICHIA COLI.
P85B_RAT PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY BETA SUBUNIT (PI3-KINASE P85-BETA SUBUNIT) (PTDINS-3-KINASE P85-BETA) - RATTUS NORVEGICUS (RAT).
P94680 TOLUENESULFONATE METHYL-MONOOXYGENASE REDUCTASE COMPONENT - COMAMONAS TESTOSTERONI (PSEUDOMONAS TESTOSTERONI).
PDR_BURCE PHTHALATE DIOXYGENASE REDUCTASE (EC 1.-.-.-) (PDR) - BURKHOLDERIA CEPACIA (PSEUDOMONAS CEPACIA).
Q09590 PROBABLE NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - CAENORHABDITIS ELEGANS.
Q12683 SULFITE REDUCTASE ALPHA SUBUNIT (EC 1.8.1.2) (SULFITE REDUCTASE (NADPH)) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Q12684 ASSIMILATORY SULFITE REDUCTASE (EC 1.8.1.2) (SULFITE REDUCTASE (NADPH)) - SACCHAROMYCES PASTORIANUS (YEAST).
Q26240 NITRIC-OXIDE SYNTHASE (EC 1.14.13.39) - RHODNIUS PROLIXUS.
Q41641 FERREDOXIN-NADP+ REDUCTASE - VOLVOX CARTERI.
Q42497 NITRATE REDUCTASE - CHLORELLA VULGARIS.
Q43042 NITRATE REDUCTASE APOENZYME (EC 1.6.6.1) (NITRATE REDUCTASE (NADH)) (ASSIMILATORY NITRATE REDUCTASE) - PETUNIA HYBRIDA (PETUNIA).
Q43235 NADPH-FERRIHEMOPROTEIN REDUCTASE (EC 1.6.2.4) (NADPH--FERRIHEMOPROTEIN REDUCTASE) (NADPH--CYTOCHROME P450 REDUCTASE) (TPNH(2) CYTOCHROME C REDUCTASE) (FERRIHEMOPROTEIN P450 REDUCTASE) - VICIA SATIVA (SPRING VETCH) (TARE).
Q58841 HYPOTHETICAL PROTEIN MJ1446 - METHANOCOCCUS JANNASCHII.
Q59668 HYDROGENASE (EC 1.12.2.1) (CYTOCHROME-C3 HYDROGENASE) - PYROCOCCUS FURIOSUS.
Q60451 NADPH-CYTOCHROME P450 OXIDOREDUCTASE - CRICETULUS GRISEUS (CHINESE HAMSTER).
Q92237 NITRATE REDUCTASE - FUSARIUM MONILIFORME (GIBBERELLA FUJIKUROI).
UBIB_ECOLI NAD(P)H-FLAVIN REDUCTASE (EC 1.6.8.-) (FERRISIDEROPHORE REDUCTASE C) - ESCHERICHIA COLI.
UBIB_VIBFI NAD(P)H-FLAVIN REDUCTASE (EC 1.6.8.-) - VIBRIO FISCHERI.
VANB_ACICA VANILLATE O-DEMETHYLASE OXIDOREDUCTASE (EC 1.14.13.-) (VANILLATE DEGRADATION FERREDOXIN-LIKE PROTEIN) - ACINETOBACTER CALCOACETICUS.
VANB_PSEPU VANILLATE O-DEMETHYLASE OXIDOREDUCTASE (EC 1.14.13.-) (VANILLATE DEGRADATION FERREDOXIN-LIKE PROTEIN) - PSEUDOMONAS PUTIDA.
VANB_PSES9 VANILLATE O-DEMETHYLASE OXIDOREDUCTASE (EC 1.14.13.-) (VANILLATE DEGRADATION FERREDOXIN-LIKE PROTEIN) - PSEUDOMONAS SP. (STRAIN ATCC 19151).
VANB_PSESP VANILLATE O-DEMETHYLASE OXIDOREDUCTASE (EC 1.14.13.-) (VANILLATE DEGRADATION FERREDOXIN-LIKE PROTEIN) - PSEUDOMONAS SP. (STRAIN HR199 / DSM 7063).
Scan History
OWL26_2    2  500  NSINGLE    
SPTR37_9f 2 500 NSINGLE
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
YLAGQYIDLIING ASCD_YERPS 129 129 -
FLPGQYINLHYKG Q17574 126 126 -
FLAGQYVNVTLPG BENC_ACICA 149 149 -
FYPGQYADVECAE XYLA_PSEPU 144 144 -
FLPGQYVNLQVPG XYLZ_PSEPU 136 136 -
FEPGQFMDLTIPG MEMC_METCA 142 142 -
FQAGQYVNLALPG DMPP_PSEPU 131 131 -

Motif 2 width=8
Element Seqn Id St Int Rpt
RSYSFSSQ BENC_ACICA 166 4 -
RAFSLANP DMPP_PSEPU 148 4 -
RSYSFSAP XYLA_PSEPU 161 4 -
RSYSIANA ASCD_YERPS 144 2 -
RAYSFSSL XYLZ_PSEPU 153 4 -
RSYSPANL MEMC_METCA 159 4 -
RSYSIANS Q17574 141 2 -

Motif 3 width=10
Element Seqn Id St Int Rpt
APYGQFGLHE XYLA_PSEPU 205 36 -
GPYGQFFVRD DMPP_PSEPU 192 36 -
GPLGAFYLRE XYLZ_PSEPU 196 35 -
GPLGVFGLKE MEMC_METCA 203 36 -
GPFGSFYLRD BENC_ACICA 209 35 -
GPCGTFFIRE Q17574 184 35 -
GPQGTFFVRE ASCD_YERPS 188 36 -

Motif 4 width=20
Element Seqn Id St Int Rpt
IFLAGGTGFAPVKSMVEHLI Q17574 199 5 -
LMLAGGTGIAPFLSMLQVLE BENC_ACICA 224 5 -
YFVAGGTGLAPVVSMVRQMQ MEMC_METCA 219 6 -
VCVAGGTGLAPIKCVLQSMT XYLA_PSEPU 220 5 -
LLLAGGTGLAPFTAMLEKIA XYLZ_PSEPU 211 5 -
IFIAGGSGLSSPQSMILDLL DMPP_PSEPU 208 6 -
VFLAGGTGFAPVKSMVEALI ASCD_YERPS 203 5 -

Motif 5 width=10
Element Seqn Id St Int Rpt
NETRIYFGVN MEMC_METCA 244 5 -
HPLHLIYGVT XYLZ_PSEPU 236 5 -
RRITLFQGAR DMPP_PSEPU 233 5 -
RDVLLFFGAR XYLA_PSEPU 245 5 -
HPVRLVFGVT BENC_ACICA 249 5 -
REIYIYWGMQ Q17574 224 5 -
RQVHIYWGMP ASCD_YERPS 228 5 -

Motif 6 width=9
Element Seqn Id St Int Rpt
DIYLCGPPG MEMC_METCA 310 56 -
DIYLCGPPP XYLZ_PSEPU 300 54 -
KAYLCGPPP DMPP_PSEPU 301 58 -
EGYLCGPPP XYLA_PSEPU 312 57 -
NVYACGSLA ASCD_YERPS 292 54 -
DIYACGSPV Q17574 288 54 -
DVYLCGPVP BENC_ACICA 313 54 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
FQAGQYINLTLPG Q52175 132 132 -
FQAGQYINLTLPG Q52166 132 132 -
FQAGQYVNLALPG Q52168 132 132 -
FQAGQYINIQLPN O32433 132 132 -
FQAGQYINIQLPN Q43983 132 132 -
FQAGQYLNLQVPG O84963 133 133 -
YLAGQYANLRVPG O33457 142 142 -
FYPGQYADVECAE O30866 143 143 -
FQAGQYVNLALPG DMPP_PSESP 131 131 -
FTPGQYADLTAPG O85971 137 137 -
FTAGQFVNVEVPG Q53028 135 135 -
FYPGQYADVECAE O52644 144 144 -
FYPGQYADVECAE XYLA_PSEPU 144 144 -
YLAGRYANVRVPG P95461 142 142 -
FLAGQYVNVTLPG BENC_ACICA 149 149 -
FLPGQFVDIEIPG RFBI_SALTY 141 141 -
FLPGQYINLHYKG Q17574 126 126 -
YLAGQYIDLIING ASCD_YERPS 129 129 -
YLPGQYVDLSFRG O31003 123 123 -

Motif 2 width=8
Element Seqn Id St Int Rpt
RAFSLANP DMPP_PSESP 148 4 -
RAFSLANP Q52175 149 4 -
RAFSLANP Q52166 149 4 -
RAFSLANP Q52168 149 4 -
RAFSIANT O32433 149 4 -
RAFSIANT Q43983 149 4 -
RAFSLANA O84963 150 4 -
RSYSFANA O33457 159 4 -
RSYSFATP O30866 160 4 -
RSYSFAFA O85971 154 4 -
RTFSLANA Q53028 152 4 -
RSYSFSAP O52644 161 4 -
RSYSFSAP XYLA_PSEPU 161 4 -
RCYSFANA P95461 159 4 -
RSYSFSSQ BENC_ACICA 166 4 -
RSYSMASV RFBI_SALTY 158 4 -
RSYSIANS Q17574 141 2 -
RSYSIANA ASCD_YERPS 144 2 -
RSYSIANA O31003 138 2 -

Motif 3 width=10
Element Seqn Id St Int Rpt
GPYGQFFVRD DMPP_PSESP 192 36 -
GPYGQFFVRD Q52175 193 36 -
GPYGQFFVRG Q52166 193 36 -
GPYGQFFVRD Q52168 193 36 -
GPYGQFFVRK O32433 193 36 -
GPYGQFFVRK Q43983 193 36 -
APYGRFFVRR O84963 193 35 -
APQGTFHLHG O33457 205 38 -
APYGQFGLHE O30866 204 36 -
APYGQFALKD O85971 200 38 -
GPYGQLKIRL Q53028 196 36 -
APYGQFGLHE O52644 205 36 -
APYGQFGLHE XYLA_PSEPU 205 36 -
APQGTFHLHG P95461 205 38 -
GPFGSFYLRD BENC_ACICA 209 35 -
GPAGSFSLHK RFBI_SALTY 201 35 -
GPCGTFFIRE Q17574 184 35 -
GPQGTFFVRE ASCD_YERPS 188 36 -
GPKGTFFVRD O31003 182 36 -

Motif 4 width=20
Element Seqn Id St Int Rpt
LCIAGGSGLAPLVSILEHAR P95461 220 5 -
IFIAGGSGLSSPQSMILDLL DMPP_PSESP 208 6 -
IFIAGGSGLSSPQSMIFDLF Q52175 209 6 -
IFIAGGSGLSSPQSMVFDLL Q52166 209 6 -
IFIAGGSGLSSPQSMILNLL Q52168 209 6 -
IFIAGGSGLSSPQSMILDLL O32433 209 6 -
IFIAGGSGLSSLQSMILDLL Q43983 209 6 -
LFLAGGSGLSSPRAMIRELL O84963 209 6 -
VCIAGGSGLAPLISILQHAR O33457 220 5 -
VCVAGGTGLAPIKCVLQSMT O30866 219 5 -
LCIAGGSGLAPIISILEQAL O85971 215 5 -
LMIAGGSGLAPLLSMLRDLA Q53028 211 5 -
VCVAGGTGLAPIKCVLQSMT O52644 220 5 -
VCVAGGTGLAPIKCVLQSMT XYLA_PSEPU 220 5 -
LMLAGGTGIAPFLSMLQVLE BENC_ACICA 224 5 -
FFVAGGTGLSPVLSMIRQLH RFBI_SALTY 216 5 -
IFLAGGTGFAPVKSMVEHLI Q17574 199 5 -
VFLAGGTGFAPVKSMVEALI ASCD_YERPS 203 5 -
IFIATGTGIAPIKAIVEELI O31003 197 5 -

Motif 5 width=10
Element Seqn Id St Int Rpt
RRITLFQGAR DMPP_PSESP 233 5 -
RQITLFQGAR Q52175 234 5 -
RQITLFQGAR Q52166 234 5 -
RRITLFQGAR Q52168 234 5 -
RIIYLFQGAR O32433 234 5 -
RIIYLFQGAR Q43983 234 5 -
LPITLVQGAR O84963 234 5 -
RDCTLLFGAR O33457 245 5 -
RDVLLFFGAR O30866 244 5 -
RAVHLLYGAR O85971 240 5 -
RPVSMFFGAR Q53028 236 5 -
RDVLLFFGAR O52644 245 5 -
RDCILLFGAR P95461 245 5 -
HPVRLVFGVT BENC_ACICA 249 5 -
RDVLLFFGAR XYLA_PSEPU 245 5 -
QPATLFFGVT RFBI_SALTY 241 5 -
REIYIYWGMQ Q17574 224 5 -
RQVHIYWGMP ASCD_YERPS 228 5 -
RNVYIYWGMR O31003 222 5 -

Motif 6 width=9
Element Seqn Id St Int Rpt
NVYACGSLA ASCD_YERPS 292 54 -
KAYLCGPPP DMPP_PSESP 301 58 -
KAYLCGPPP Q52175 302 58 -
KAYLCGPPP Q52166 302 58 -
KAYLCGPPP Q52168 302 58 -
KAYLCGPPP O32433 302 58 -
KAYLCGPPP Q43983 302 58 -
KAYLCGPPP O84963 307 63 -
EGYLCGPPP O33457 311 56 -
EGYLCGPPP O30866 311 57 -
EAYLCGPPA O85971 307 57 -
DVYLCGPPP Q53028 301 55 -
EGYLCGPPP O52644 312 57 -
EGYLCGPPP XYLA_PSEPU 312 57 -
EGYLCGPPP P95461 311 56 -
DVYLCGPVP BENC_ACICA 313 54 -
DIYLCGPPG RFBI_SALTY 307 56 -
DIYACGSPV Q17574 288 54 -
EVYACGSSK O31003 287 55 -