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PR00406

Identifier
CYTB5RDTASE  [View Relations]  [View Alignment]  
Accession
PR00406
No. of Motifs
6
Creation Date
03-OCT-1995  (UPDATE 14-JUN-1999)
Title
Cytochrome B5 reductase signature
Database References

PROSITE; PS00191 CYTOCHROME_B5
INTERPRO; IPR001834
PDB; 1CND; 1NDH
SCOP; 1NDH
Literature References
1. HYDE, G.E., CRAWFORD, N.M. AND CAMPBELL, W.H.
The sequence of squash NADH:nitrate reductase and its relationship to the
sequences of other flavoprotein oxidoreductases. A family of flavoprotein
pyridine nucleotide cytochrome reductases.
J.BIOL.CHEM. 266 23542-23547 (1991).
 
2. KARPLUS, P.A. AND BRUNS, C.M.
Structure-function relations for ferredoxin reductase.
J.BIOENERG.BIOMEMBR. 26 89-99 (1994).
 
3. CAMPBELL, W.H. AND KINGHORN, J.R.
Functional domains of assimilatory nitrate and nitrite reductases.
TRENDS BIOCHEM.SCI. 15 315-319 (1990).
 
4. TAMURA, M., YUBISUI, T., TAKESHITA, M., KAWABATA, S., MIYATA, T. AND
IWANAGA, S.
Structural comparison of bovine erythrocyte, brain, and liver 
NADH-cytochrome b5 reductase by HPLC mapping.
J.BIOCHEM.(TOKYO) 101 1147-1159 (1987).
 
5. OZOLS, J., CARR, S.A. AND STRITTMATTER, P. 
Identification of the NH2-terminal blocking group of NADH-cytochrome b5
reductase as myristic acid and the complete amino acid sequence of the 
membrane-binding domain. 
J.BIOL.CHEM. 259 13349-13354 (1984).
 
6. YUBISUI, T., MURAKAMI, K., SHIRABE, K., TAKESHITA, M., ZENNO, S., 
TOMATSU, S. AND FUKUMAKI, Y.
Structural analysis of NADH-cytochrome b5 reductase in relation to 
hereditary methemoglobinemia.
PROG.CLIN.BIOL.RES. 319 107-119 (1989).
 
7. LU, G., CAMPBELL, W.H., SCHNEIDER, G. AND LINDQVIST, Y.
Crystal structure of the FAD-containing fragment of corn nitrate reductase
at 2.5 A resolution: relationship to other flavoprotein reductases.
STRUCTURE 2 809-821 (1994).
 
8. NISHIDA, H., INAKA, K., YAMANAKA, M., KAIDA, S., KOBAYASHI, K. AND 
MIKI, K.
Crystal structure of NADH-cytochrome b5 reductase from pig liver at 2.4A
resolution. 
BIOCHEMISTRY 34 2763-2767 (1995). 

Documentation
Flavoprotein pyridine nucleotide cytochrome reductases [1] (FPNCR) catalyse
the interchange of reducing equivalents between one-electron carriers and
the two-electron-carrying nicotinamide dinucleotides. The enzymes include
ferredoxin:NADP+ reductases (FNR) [2]; plant and fungal NAD(P)H:nitrate
reductases [1,3]; NADH:cytochrome b5 reductases [4]; NADPH:P450 reductases;
NADPH:sulphite reductases; nitric oxide synthases; phthalate dioxygenase 
reductase; and various other flavoproteins.
 
NADH:cytochrome b5 reductase (CBR) serves as electron donor for cytochrome
b5, a ubiquitous electron carrier (see signature CYTOCHROMEB5), thus 
participating in a variety of metabolic pathways (including steroid 
biosynthesis, desaturation and elongation of fatty acids, P450-dependent
reactions, methaemoglobin reduction, etc.). A membrane-bound form of CBR is
located on the cytosolic side of the endoplasmic reticulum, while a soluble
form is found in erythrocytes [4]. In the membrane-bound form, the N-
terminal residue is myristoylated [5]. Deficiency of the erythrocyte form
causes hereditary methaemoglobinemia [6].
 
In biological nitrate assimilation, reduction of nitrate to nitrite is
catalysed by the multidomain redox enzyme NAD(P)H:nitrate reductase (NR).
Three forms of NR are known: an NADH-specific enzyme found in higher plants
and algae (EC 1.6.6.1); an NAD(P)H-bispecific enzyme found in higher plants,
algae and fungi (EC 1.6.6.2); and an NADPH-specific enzyme found only in
fungi (EC 1.6.6.3) [3]. NR can be divided into 3 structure/function domains:
the molybdopterin cofactor binds in the N-terminal domain; the central
region is the cytochrome b domain, which is similar to animal cytochrome b5
(see signature CYTOCHROMEB5); and the C-terminal portion of the protein is
occupied by the FAD/NAD(P)H binding domain, which is similar to CBR [3].
The catalytic reduction of nitrate to nitrite can be viewed as a single
polypeptide electron transport chain with electron flow from NAD(P)H ->
FAD -> cytochrome b5 -> molybdopterin -> NO(3). Thus, the flavin domain of
NR is functionally identical to CBR.  
 
To date, the 3D-structures of the flavoprotein domain of corn nitrate
reductase [7] and of pig NADH:cytochrome b5 reductase [8] have been solved.
The overall fold is similar to that of ferredoxin:NADP+ reductase [2]: 
the FAD-binding domain (N-terminal) has the topology of an anti-parallel 
beta-barrel, while the NAD(P)-binding domain (C-terminal) has the topology 
of a classical pyridine dinucleotide-binding fold (i.e., a central parallel 
beta-sheet flanked by 2 helices on each side).
 
CYTB5RDTASE is a 6-element fingerprint that provides a signature for the
cytochrome b5 reductase family. The fingerprint was derived from an initial
alignment of 12 sequences - the motifs were drawn from short conserved
regions spanning virtually the full alignment length - motifs 1-3 lie
in the beta-barrel domain, and motifs 4-6 in the NAD(P)-binding domain. 
Motif 2 is involved in binding the isoalloxazine ring of FAD (cf. motif 2
of FPNCR); motif 4 includes residues involved in binding the diphosphate
group of the pyridine nucleotide (cf. motif 4 of FPNCR); motif 5
corresponds to motif 6 of FPNCR; and motif 6 contains a conserved Cys
residue that may interact with NAD(P)H, and is necessary for efficient
electron transfer (cf. motif 8 of FPNCR). Two iterations on OWL26.2 were
required to reach convergence, at which point a true set comprising 47
sequences was identified. Several partial matches were also found: a
hypothetical yeast protein (S54059), identified as a putative CBR, matches
motifs 2-5; 2 hypothetical yeast proteins, similar to NR (YKP0_YEAST and
S49640), match motifs 2, 4, 5, 6 and 3-6, respectively; NIA_LOTTE is a
C-terminal fragment of NR matching motifs 4-6; FNR from Anabaena 
(FENR_ANASO) matches motifs 3-5; 2 [2Fe-2S] ferredoxin/FNR fusion proteins
(MEMC_METCA and XYLZ_PSEPU) match motifs 3, 4 and 6; plant FNRs 
(FENR_ORYSA, FENS_ORYSA, FENR_PEA, FENR_VICFA, FENR_MESCR and S53305),
bacterial [2Fe-2S] ferredoxin/FNR fusion proteins (C48360 and PPU040525),
and the reductase component of the phenoxybenzoate dioxygenase system 
(S44172) match motifs 3 and 4; animal P450 reductases (NCPR_RAT, RATNCPR6,
NCPR_CAVPO, NCPR_HUMAN, NCPR_MOUSE, NCPR_PIG and NCPR_RABIT) match motifs
4 and 5; cytochrome-c3 hydrogenase (S48834) matches motifs 3 and 5; and
[2Fe-2S] ferredoxin/FNR fusion protein (PCCBDABC3) matches motifs 4 and 6.
 
An update on SPTR37_9f identified a true set of 48 sequences, and 23
partial matches.
Summary Information
  48 codes involving  6 elements
1 codes involving 5 elements
1 codes involving 4 elements
3 codes involving 3 elements
18 codes involving 2 elements
Composite Feature Index
6484848484848
5110111
4001111
3012321
20491409
123456
True Positives
NC5R_BOVIN    NC5R_HUMAN    NC5R_RAT      NC5R_YEAST    
NIA1_ARATH NIA1_BRANA NIA1_HORVU NIA1_ORYSA
NIA1_PHAVU NIA1_SOYBN NIA1_TOBAC NIA2_ARATH
NIA2_BRANA NIA2_PHAVU NIA2_SOYBN NIA2_TOBAC
NIA3_MAIZE NIA7_HORVU NIA_ASPNG NIA_BEABA
NIA_BETVE NIA_CICIN NIA_CUCMA NIA_EMENI
NIA_FUSOX NIA_LEPMC NIA_LOTJA NIA_LYCES
NIA_NEUCR NIA_PETHY NIA_PHYIN NIA_PICAN
NIA_SPIOL NIA_USTMA NIA_VOLCA O00101
O04926 O13486 O16521 O16522
O24390 O74557 Q00303 Q01696
Q12561 Q42497 Q43042 Q92237
True Positive Partials
Codes involving 5 elements
Q00284
Codes involving 4 elements
YMI7_YEAST
Codes involving 3 elements
MCR1_YEAST XYLZ_PSEPU YMM5_YEAST
Codes involving 2 elements
FENS_ORYSA FENS_PEA FENS_TOBAC FENT_ORYSA
MEMC_METCA O05933 O53013 O86347
P76081 P77233 P78476 P78477
P94680 P95533 Q51603 Q52574
Q53028 TTC3_HUMAN
Sequence Titles
NC5R_BOVIN  NADH-CYTOCHROME B5 REDUCTASE (EC 1.6.2.2) - BOS TAURUS (BOVINE). 
NC5R_HUMAN NADH-CYTOCHROME B5 REDUCTASE (EC 1.6.2.2) (B5R) - HOMO SAPIENS (HUMAN).
NC5R_RAT NADH-CYTOCHROME B5 REDUCTASE (EC 1.6.2.2) - RATTUS NORVEGICUS (RAT).
NC5R_YEAST PUTATIVE NADH-CYTOCHROME B5 REDUCTASE (EC 1.6.2.2) (P35) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
NIA1_ARATH NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR1) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
NIA1_BRANA NITRATE REDUCTASE, CLONE PBNBR1405 (EC 1.6.6.1) (NR) - BRASSICA NAPUS (RAPE).
NIA1_HORVU NITRATE REDUCTASE (EC 1.6.6.1) (NR) - HORDEUM VULGARE (BARLEY).
NIA1_ORYSA NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR1) - ORYZA SATIVA (RICE).
NIA1_PHAVU NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR-1) - PHASEOLUS VULGARIS (KIDNEY BEAN) (FRENCH BEAN).
NIA1_SOYBN INDUCIBLE NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR) - GLYCINE MAX (SOYBEAN).
NIA1_TOBAC NITRATE REDUCTASE 1 (EC 1.6.6.1) (NR1) - NICOTIANA TABACUM (COMMON TOBACCO).
NIA2_ARATH NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR2) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
NIA2_BRANA NITRATE REDUCTASE, CLONE PBNBR1412 (EC 1.6.6.1) (NR) - BRASSICA NAPUS (RAPE).
NIA2_PHAVU NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR-2) - PHASEOLUS VULGARIS (KIDNEY BEAN) (FRENCH BEAN).
NIA2_SOYBN INDUCIBLE NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR) - GLYCINE MAX (SOYBEAN).
NIA2_TOBAC NITRATE REDUCTASE 2 (EC 1.6.6.1) (NR2) - NICOTIANA TABACUM (COMMON TOBACCO).
NIA3_MAIZE NITRATE REDUCTASE 3 (EC 1.6.6.1) (NR) - ZEA MAYS (MAIZE).
NIA7_HORVU NITRATE REDUCTASE [NAD(P)H] (EC 1.6.6.2) - HORDEUM VULGARE (BARLEY).
NIA_ASPNG NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - ASPERGILLUS NIGER.
NIA_BEABA NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - BEAUVERIA BASSIANA (TRITIRACHIUM SHIOTAE).
NIA_BETVE NITRATE REDUCTASE [NAD(P)H] (EC 1.6.6.2) (NR) - BETULA VERRUCOSA (WHITE BIRCH) (BETULA PENDULA).
NIA_CICIN NITRATE REDUCTASE (EC 1.6.6.1) (NR) - CICHORIUM INTYBUS (CHICORY).
NIA_CUCMA NITRATE REDUCTASE (EC 1.6.6.1) (NR) - CUCURBITA MAXIMA (PUMPKIN) (WINTER SQUASH).
NIA_EMENI NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - EMERICELLA NIDULANS (ASPERGILLUS NIDULANS).
NIA_FUSOX NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - FUSARIUM OXYSPORUM.
NIA_LEPMC NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - LEPTOSPHAERIA MACULANS (BLACKLEG FUNGUS).
NIA_LOTJA NITRATE REDUCTASE (EC 1.6.6.1) (NR) - LOTUS JAPONICUS.
NIA_LYCES NITRATE REDUCTASE (EC 1.6.6.1) (NR) - LYCOPERSICON ESCULENTUM (TOMATO).
NIA_NEUCR NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - NEUROSPORA CRASSA.
NIA_PETHY NITRATE REDUCTASE (EC 1.6.6.1) (NR) - PETUNIA HYBRIDA (PETUNIA).
NIA_PHYIN NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - PHYTOPHTHORA INFESTANS (POTATO LATE BLIGHT FUNGUS).
NIA_PICAN NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - PICHIA ANGUSTA (YEAST) (HANSENULA POLYMORPHA).
NIA_SPIOL NITRATE REDUCTASE (EC 1.6.6.1) (NR) - SPINACIA OLERACEA (SPINACH).
NIA_USTMA NITRATE REDUCTASE [NADPH] (EC 1.6.6.3) (NR) - USTILAGO MAYDIS (SMUT FUNGUS).
NIA_VOLCA NITRATE REDUCTASE (EC 1.6.6.1) (NR) - VOLVOX CARTERI.
O00101 NITRATE REDUCTASE (NADPH) (EC 1.6.6.3) - PHAEOSPHAERIA NODORUM (SEPTORIA NODORUM).
O04926 NADH NITRATE REDUCTASE (EC 1.6.6.3) (NITRATE REDUCTASE (NADPH)) - SOLANUM TUBEROSUM (POTATO).
O13486 NITRATE REDUCTASE - METARHIZIUM ANISOPLIAE.
O16521 T05H4.5 PROTEIN - CAENORHABDITIS ELEGANS.
O16522 T05H4.4 PROTEIN - CAENORHABDITIS ELEGANS.
O24390 NADH NITRATE REDUCTASE (EC 1.6.6.3) - SOLANUM TUBEROSUM (POTATO).
O74557 PUTATIVE NADH-CYTOCHROME B5 REDUCTASE - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
Q00303 NITRATE REDUCTASE - BOTRYTIS CINEREA (BOTRYOTINIA FUCKELIANA).
Q01696 NITRATE REDUCTASE - PENICILLIUM CHRYSOGENUM.
Q12561 NITRATE REDUCTASE (EC 1.7.99.4) - ASPERGILLUS ORYZAE.
Q42497 NITRATE REDUCTASE - CHLORELLA VULGARIS.
Q43042 NITRATE REDUCTASE APOENZYME (EC 1.6.6.1) (NITRATE REDUCTASE (NADH)) (ASSIMILATORY NITRATE REDUCTASE) - PETUNIA HYBRIDA (PETUNIA).
Q92237 NITRATE REDUCTASE - FUSARIUM MONILIFORME (GIBBERELLA FUJIKUROI).

Q00284 NITRATE REDUCTASE - ASPERGILLUS PARASITICUS.

YMI7_YEAST HYPOTHETICAL 35.8 KD PROTEIN IN RPM2-TUB1 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).

MCR1_YEAST NADH-CYTOCHROME B5 REDUCTASE PRECURSOR (EC 1.6.2.2) (P34/P32) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
XYLZ_PSEPU TOLUATE 1,2-DIOXYGENASE ELECTRON TRANSFER COMPONENT [INCLUDES: FERREDOXIN; FERREDOXIN--NAD(+) REDUCTASE (EC 1.18.1.3)] - PSEUDOMONAS PUTIDA.
YMM5_YEAST HYPOTHETICAL 35.3 KD PROTEIN IN HMGS-TUB3 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).

FENS_ORYSA FERREDOXIN--NADP REDUCTASE, ROOT ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - ORYZA SATIVA (RICE).
FENS_PEA FERREDOXIN--NADP REDUCTASE, ROOT ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - PISUM SATIVUM (GARDEN PEA).
FENS_TOBAC FERREDOXIN--NADP REDUCTASE, ROOT-TYPE ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - NICOTIANA TABACUM (COMMON TOBACCO).
FENT_ORYSA FERREDOXIN--NADP REDUCTASE, EMBRYO ISOZYME PRECURSOR (EC 1.18.1.2) (FNR) - ORYZA SATIVA (RICE).
MEMC_METCA METHANE MONOOXYGENASE COMPONENT C (EC 1.14.13.25) (METHANE HYDROXYLASE) - METHYLOCOCCUS CAPSULATUS.
O05933 2-OXO-1,2-DIHYDROQUINOLINE 8-MONOOXYGENASE, REDUCTASE COMPONENT - PSEUDOMONAS PUTIDA.
O53013 FERREDOXIN REDUCTASE ELECTRON TRANSFER COMPONENT - ESCHERICHIA COLI.
O86347 HYPOTHETICAL 33.5 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
P76081 FROM BASES 1449468 TO 1461540 (SECTION 126 OF 400) OF THE COMPLETE GENOME (SECTION 126 OF 400) - ESCHERICHIA COLI.
P77233 FERREDOXIN - ESCHERICHIA COLI.
P78476 TETRATRICOPEPTIDE REPEAT DOMAIN 3 (TPR REPEAT PROTEIN D) - HOMO SAPIENS (HUMAN).
P78477 TETRATRICOPEPTIDE REPEAT DOMAIN 3 (TPR REPEAT PROTEIN D) - HOMO SAPIENS (HUMAN).
P94680 TOLUENESULFONATE METHYL-MONOOXYGENASE REDUCTASE COMPONENT - COMAMONAS TESTOSTERONI (PSEUDOMONAS TESTOSTERONI).
P95533 ELECTRON TRANSFER PROTEIN - PSEUDOMONAS PUTIDA.
Q51603 2-HALOBENZOATE 1,2-DIOXYGENASE - BURKHOLDERIA CEPACIA (PSEUDOMONAS CEPACIA).
Q52574 OXIDOREDUCTASE - PSEUDOMONAS SP.
Q53028 REDUCTASE - NOCARDIA CORALLINA.
TTC3_HUMAN TETRATRICOPEPTIDE REPEAT DOMAIN 3 (TPR REPEAT PROTEIN D) - HOMO SAPIENS (HUMAN).
Scan History
OWL26_2    2  500  NSINGLE    
SPTR37_9f 2 250 NSINGLE
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
LGLPVGKHIFVC NIA1_ORYSA 687 687 -
LGLPVGKHVFLC NIA_BETVE 673 673 -
LGLPVGKHVFVC NIA1_ARATH 691 691 -
IGLPVGKHVFTY NIA_VOLCA 637 637 -
LGLPVGQHIYLS NC5R_HUMAN 70 70 -
LGLPVGQHIYLS NC5R_BOVIN 70 70 -
LGLPIGQHIYLS NC5R_RAT 70 70 -
LGLPTGQHLMIR NIA_LEPMC 672 672 -
LGLPTGQHLMLK NIA_EMENI 647 647 -
LGLPIGQHLMIK NIA_ASPNG 646 646 -
LGLPWPAALRLS NIA_USTMA 648 648 -
LGLPIGQHIVIK NC5R_YEAST 107 107 -

Motif 2 width=8
Element Seqn Id St Int Rpt
RAYTPTSM NIA1_ORYSA 708 9 -
RAYTPTST NIA_BETVE 694 9 -
RAYTPTSA NIA1_ARATH 712 9 -
RAYTPISG NIA_VOLCA 658 9 -
RPYTPISS NC5R_HUMAN 91 9 -
RPYTPVSS NC5R_BOVIN 91 9 -
RPYTPVSS NC5R_RAT 91 9 -
RSYTPISQ NIA_LEPMC 695 11 -
RSYTPISP NIA_EMENI 672 13 -
RSYTPISD NIA_ASPNG 669 11 -
RAYTPYSG NIA_USTMA 673 13 -
RSYTPTSL NC5R_YEAST 128 9 -

Motif 3 width=15
Element Seqn Id St Int Rpt
GAYIDVKGPLGHVEY NIA1_ORYSA 753 37 -
GSVLHVKGPLGHVEY NIA_BETVE 739 37 -
GSMIDIKGPLGHIEY NIA1_ARATH 757 37 -
GDTVEFKGPLGHFVY NIA_VOLCA 703 37 -
GDTIEFRGPSGLLVY NC5R_HUMAN 136 37 -
GDTIEFRGPNGLLVY NC5R_BOVIN 136 37 -
GDTIEFRGPNGLLVY NC5R_RAT 136 37 -
GHWVDMKGPIGKFEY NIA_LEPMC 737 34 -
GSVIECKGPTGRFEY NIA_EMENI 714 34 -
GSEIDCKGPTGRFEY NIA_ASPNG 711 34 -
DLTIELKGPLGSFTY NIA_USTMA 727 46 -
GDSIQIKGPRGNYHY NC5R_YEAST 165 29 -

Motif 4 width=20
Element Seqn Id St Int Rpt
AMIAGGSGITPMYQVIQSVL NIA1_ORYSA 786 18 -
AMVAGGTGITPIYQVIQAIL NIA_BETVE 772 18 -
AMLAGGTGITPIYQIIQSIL NIA1_ARATH 790 18 -
SFVAGGTGITPCYAVIKAAL NIA_VOLCA 737 19 -
GMIAGGTGITPMLQVIRAIM NC5R_HUMAN 175 24 -
GMIAGGTGITPMLQVIRAIM NC5R_BOVIN 175 24 -
GMIAGGTGITPMLQVIRAVL NC5R_RAT 175 24 -
KMICGGSGITPIYQVLRAIL NIA_LEPMC 770 18 -
VMICGGTGITPVFQVLRAVM NIA_EMENI 747 18 -
KMICGGTGITPIFQVLRAVM NIA_ASPNG 744 18 -
AMIAGGSGITPIWSTLKAIA NIA_USTMA 761 19 -
GMIAGGTGIAPMYQIMKAIA NC5R_YEAST 188 8 -

Motif 5 width=12
Element Seqn Id St Int Rpt
DDILLRDELDRW NIA1_ORYSA 825 19 -
DDILLREELDDW NIA_BETVE 810 18 -
DDILVREELEGW NIA1_ARATH 828 18 -
EDILLREELDEL NIA_VOLCA 775 18 -
KDILLRPELEEL NC5R_HUMAN 213 18 -
KDILLRPELEEL NC5R_BOVIN 213 18 -
KDILLRPELEEL NC5R_RAT 213 18 -
EDILCREDLDRF NIA_LEPMC 808 18 -
EDILLKNELDEF NIA_EMENI 785 18 -
EDILCRADLDAY NIA_ASPNG 782 18 -
QDILIREELDAL NIA_USTMA 809 28 -
EDILLKKELEAL NC5R_YEAST 226 18 -

Motif 6 width=9
Element Seqn Id St Int Rpt
LALACGPPP NIA1_ORYSA 884 47 -
LALACGAPS NIA_BETVE 866 44 -
LALACGPPP NIA1_ARATH 885 45 -
LALMCGPPA NIA_VOLCA 833 46 -
LVLMCGPPP NC5R_HUMAN 269 44 -
LVLMCGPPP NC5R_BOVIN 269 44 -
LILMCGPPP NC5R_RAT 269 44 -
LVLVCGPEG NIA_LEPMC 863 43 -
MVLVCGPEA NIA_EMENI 843 46 -
MVLICGPEA NIA_ASPNG 837 43 -
LALVCGPPP NIA_USTMA 877 56 -
QILICGPPA NC5R_YEAST 284 46 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
LGLPVGKHIFLC NIA1_TOBAC 678 678 -
LGLPVGKHIFLC NIA2_TOBAC 678 678 -
LGLPVGKHIFLC NIA_LOTJA 675 675 -
LGLPVGKHIFLC O24390 685 685 -
LGLPVGKHIFLC O04926 685 685 -
LGLPVGKHIFLC NIA_PETHY 683 683 -
LGLPVGKHIFLC Q43042 689 689 -
LGLPVGKHIFLC NIA_LYCES 685 685 -
LGLPVGKHIFIC NIA_CUCMA 693 693 -
LGLPVGKHIFLC NIA2_PHAVU 665 665 -
LGLPVGKHIFLC NIA_SPIOL 701 701 -
MGLPVGKHIFLC NIA2_SOYBN 665 665 -
LGLPVGKHIFLC NIA1_BRANA 685 685 -
MGLAVGKHIFLC NIA1_SOYBN 661 661 -
MGLPVGNHVFLC NIA1_PHAVU 656 656 -
LGLPVGKHIFVC NIA1_ORYSA 687 687 -
LGLPVGKHIFLC NIA2_BRANA 685 685 -
LGLPVGKHIFVC NIA3_MAIZE 661 661 -
LGLPVGKHIFVC NIA1_HORVU 685 685 -
LGLPVGKHVFLC NIA_BETVE 673 673 -
LGLPVGKHVFVC NIA1_ARATH 691 691 -
LGLPVGKHIFLC NIA2_ARATH 691 691 -
LGLPVGKHVFVC NIA_CICIN 694 694 -
LGLPVGKHVYVC NIA7_HORVU 661 661 -
IGLPVGKHVFTY NIA_VOLCA 637 637 -
FGLPVGKHVFLY Q42497 654 654 -
LGLPVGQHIYLS NC5R_HUMAN 70 70 -
SGLPTGKHLFLR NIA_PICAN 633 633 -
LGLPVGQHIYLS NC5R_BOVIN 70 70 -
LGLPVGQHLMIK Q12561 646 646 -
LGLPIGQHIYLS NC5R_RAT 70 70 -
LGLPIGQHVYLT O16521 79 79 -
LGLPTGQHLMIR NIA_LEPMC 672 672 -
LGLPTGQHLMLK NIA_EMENI 647 647 -
IGLPVGQHLMMR Q00303 671 671 -
IGLPTGQHLMMR Q92237 681 681 -
LGLPIGHHVYLS O16522 73 73 -
VGLPVGQHLMMR O13486 668 668 -
LGLPIGQHLMIK Q01696 643 643 -
LGLPIGQHLMIK NIA_ASPNG 646 646 -
IGLPTGQHLMMR NIA_FUSOX 679 679 -
IGLPTGQHLLVR NIA_BEABA 669 669 -
LGLPTGQHLMIR O00101 668 668 -
LGLPWPAALRLS NIA_USTMA 648 648 -
LGLPIGNHVFLY NIA_PHYIN 668 668 -
LGLPIGQHIVIK NC5R_YEAST 107 107 -
IGLPVGQHLMMR NIA_NEUCR 752 752 -
LGLPIGQHLKVF O74557 90 90 -

Motif 2 width=8
Element Seqn Id St Int Rpt
RAYTPTST NIA1_TOBAC 699 9 -
RAYTPTST NIA2_TOBAC 699 9 -
RAYTPTSG NIA_LOTJA 696 9 -
RAYTPTST O24390 706 9 -
RAYTPTST O04926 706 9 -
RAYTPTST NIA_PETHY 704 9 -
RAYTPTST Q43042 710 9 -
RAYTPTST NIA_LYCES 706 9 -
RAYTPTSS NIA_CUCMA 714 9 -
RAYTPTSS NIA2_PHAVU 686 9 -
RAYTPSST NIA_SPIOL 722 9 -
RAYTPTSS NIA2_SOYBN 686 9 -
RAYTPTST NIA1_BRANA 706 9 -
RAYTPTSS NIA1_SOYBN 682 9 -
RAYTPTSS NIA1_PHAVU 677 9 -
RAYTPTSM NIA1_ORYSA 708 9 -
RAYTPTST NIA2_BRANA 706 9 -
RAYTPTSS NIA3_MAIZE 682 9 -
RAYTPTSM NIA1_HORVU 706 9 -
RAYTPTST NIA_BETVE 694 9 -
RAYTPTSA NIA1_ARATH 712 9 -
RAYTPSST NIA2_ARATH 712 9 -
RAYTPTST NIA_CICIN 715 9 -
RAYTPTSS NIA7_HORVU 682 9 -
RAYTPISG NIA_VOLCA 658 9 -
RAYTPSSS Q42497 675 9 -
RPYTPISS NC5R_HUMAN 91 9 -
RAYTPKSS NIA_PICAN 655 10 -
RPYTPVSS NC5R_BOVIN 91 9 -
RSYTPISE Q12561 670 12 -
RPYTPVSS NC5R_RAT 91 9 -
RPYTPVSS O16521 100 9 -
RSYTPISQ NIA_LEPMC 695 11 -
RSYTPISP NIA_EMENI 672 13 -
RSYTPLSL Q00303 694 11 -
RSYTRYSD Q92237 704 11 -
RSYTPVSC O16522 94 9 -
RAYTPISE O13486 691 11 -
RSYTPMSD Q01696 666 11 -
RSYTPISD NIA_ASPNG 669 11 -
RSYTPYSD NIA_FUSOX 702 11 -
RAYTPLSE NIA_BEABA 692 11 -
ASYTPISQ O00101 691 11 -
RAYTPYSG NIA_USTMA 673 13 -
RAYTPISS NIA_PHYIN 689 9 -
RSYTPTSL NC5R_YEAST 128 9 -
RAYTPISD NIA_NEUCR 776 12 -
RSYTPLSS O74557 111 9 -

Motif 3 width=15
Element Seqn Id St Int Rpt
GSFLDVKGPLGHIEY NIA1_TOBAC 744 37 -
GSFLDVKGPLGHIEY NIA2_TOBAC 744 37 -
GSDLDVKGPLGHIEY NIA_LOTJA 741 37 -
GAFLDVKGPLGHIEY O24390 751 37 -
GAFLDVKGPLGHIEY O04926 751 37 -
GAFVDVKGPLGHIEY NIA_PETHY 749 37 -
GAFVDVKGPLGHIEY Q43042 755 37 -
GAFLDVKGPLGHIEY NIA_LYCES 751 37 -
GSTLDVKGPLGHIEY NIA_CUCMA 759 37 -
GSMLDVKGPLGHIEY NIA2_PHAVU 731 37 -
GSIVDVKGPLGHIEY NIA_SPIOL 767 37 -
GSVLDVKGPLGHIVY NIA2_SOYBN 731 37 -
GAVLDIKGPLGHIEY NIA1_BRANA 751 37 -
GSVLDVKGPLGHIEY NIA1_SOYBN 727 37 -
GSVVDVKGPLGHIEY NIA1_PHAVU 722 37 -
GAYIDVKGPLGHVEY NIA1_ORYSA 753 37 -
GSVLNIKGPLGHIEY NIA2_BRANA 751 37 -
GAHVDVKGPLGHVEY NIA3_MAIZE 727 37 -
SSYIDVKGPLGHVEY NIA1_HORVU 752 38 -
GSVLHVKGPLGHVEY NIA_BETVE 739 37 -
GSMIDIKGPLGHIEY NIA1_ARATH 757 37 -
GSTLEIKGPLGHVEY NIA2_ARATH 757 37 -
GSSIEIKGPLGHIEY NIA_CICIN 760 37 -
GAPVDIKGPVGHIEY NIA7_HORVU 727 37 -
GDTVEFKGPLGHFVY NIA_VOLCA 703 37 -
GDFMEVKGPLGHVHY Q42497 720 37 -
GDTIEFRGPSGLLVY NC5R_HUMAN 136 37 -
GNQIEVKGPVGEFEY NIA_PICAN 698 35 -
GDTIEFRGPNGLLVY NC5R_BOVIN 136 37 -
GSVVECKGPTGRFEY Q12561 712 34 -
GDTIEFRGPNGLLVY NC5R_RAT 136 37 -
GDTINFRGPQGNIVY O16521 145 37 -
GHWVDMKGPIGKFEY NIA_LEPMC 737 34 -
GSVIECKGPTGRFEY NIA_EMENI 714 34 -
GHSIDFKGPIGKFEY Q00303 736 34 -
GHWVDFKGPTGKFVY Q92237 746 34 -
GDTVSFRGPHGSIIY O16522 139 37 -
GHFVDFKGPVGKFEY O13486 733 34 -
GSKIDCKGPTGRFEY Q01696 708 34 -
GSEIDCKGPTGRFEY NIA_ASPNG 711 34 -
GHWVDFKGPTGKFVY NIA_FUSOX 744 34 -
GHFVDIKGPVGKFEY NIA_BEABA 734 34 -
GHFVDFKGPIGKFEY O00101 733 34 -
DLTIELKGPLGSFTY NIA_USTMA 727 46 -
GQQIQIKGPLGHFTY NIA_PHYIN 736 39 -
GDSIQIKGPRGNYHY NC5R_YEAST 165 29 -
GKAVEFKGPVGKFVY NIA_NEUCR 821 37 -
GDTIGVRGPKGNWKH O74557 147 28 -

Motif 4 width=20
Element Seqn Id St Int Rpt
AMIAGGTGITPVYQVMQAIL NIA1_TOBAC 777 18 -
AMIAGGTGITPVYQVMQAIL NIA2_TOBAC 777 18 -
AMLAGGTGITPIYQVAQAIL NIA_LOTJA 774 18 -
AMIAGGTGITPVYQVIESIL O24390 784 18 -
AMIAGGTGITPVYQVIESIL O04926 784 18 -
AMLAGGTGITPVYQVMQAIL NIA_PETHY 782 18 -
AMLAGGTGITPVYQVMQAIL Q43042 788 18 -
AMIAGGTGITPVYQVMQSIL NIA_LYCES 784 18 -
AMLAGGTGITPIYQVVQAIL NIA_CUCMA 792 18 -
AMLAGGTGITPIYQVAQAIL NIA2_PHAVU 764 18 -
AMISGGTGITPIYQVMQAIL NIA_SPIOL 800 18 -
AMLAGGTGITPIYQVVRAIL NIA2_SOYBN 764 18 -
AMLAGGTGITPIYQVIQSIL NIA1_BRANA 784 18 -
AMLAGGTGITPIYQVVQAIL NIA1_SOYBN 760 18 -
TMLAGGTGITPIYQVVQAIL NIA1_PHAVU 755 18 -
AMIAGGSGITPMYQVIQSVL NIA1_ORYSA 786 18 -
AMLAGGPGITPIYQVIQSIL NIA2_BRANA 784 18 -
AMVAGGSGITPIYQVIQAVL NIA3_MAIZE 760 18 -
AMICGGSGITPMYQVIQAVL NIA1_HORVU 785 18 -
AMVAGGTGITPIYQVIQAIL NIA_BETVE 772 18 -
AMLAGGTGITPIYQIIQSIL NIA1_ARATH 790 18 -
AMLAGGTGITPVYQIIQAIL NIA2_ARATH 790 18 -
AMFAGGTGITPDLSSDASYL NIA_CICIN 793 18 -
AMVAGGTGITPVYQVIQAVL NIA7_HORVU 760 18 -
SFVAGGTGITPCYAVIKAAL NIA_VOLCA 737 19 -
SMIAGGTGITPMLQVIKAVL Q42497 753 18 -
GMIAGGTGITPMLQVIRAIM NC5R_HUMAN 175 24 -
VMISGGSGITPTYQVLQAIF NIA_PICAN 731 18 -
GMIAGGTGITPMLQVIRAIM NC5R_BOVIN 175 24 -
KMICGGTGITPIFQVLRAVV Q12561 745 18 -
GMIAGGTGITPMLQVIRAVL NC5R_RAT 175 24 -
SMIAGGTGITPMLQVIAAIL O16521 184 24 -
KMICGGSGITPIYQVLRAIL NIA_LEPMC 770 18 -
VMICGGTGITPVFQVLRAVM NIA_EMENI 747 18 -
VMICGGSGVTPIFSVLRAVL Q00303 769 18 -
IMVCGGSGITPIRQVLRAIM Q92237 779 18 -
SMIAGGTGITPMLQVIAAIL O16522 178 24 -
NMICGGSGITPIFQVFRAMI O13486 766 18 -
KMICGGTGITPVFQVLRAVM Q01696 741 18 -
KMICGGTGITPIFQVLRAVM NIA_ASPNG 744 18 -
IMVCGGSGITPIRQVLRAVI NIA_FUSOX 777 18 -
VMICAGSGVTPIFQVLRAVT NIA_BEABA 767 18 -
YMVCGGSGITPIFQVLRAVM O00101 766 18 -
AMIAGGSGITPIWSTLKAIA NIA_USTMA 761 19 -
GFVAGGTGITPVYQVMRAIL NIA_PHYIN 769 18 -
GMIAGGTGIAPMYQIMKAIA NC5R_YEAST 188 8 -
VMVCGGSGVTPIYQVAEAVA NIA_NEUCR 854 18 -
GMIAGGTGITPMLQIIRAVL O74557 170 8 -

Motif 5 width=12
Element Seqn Id St Int Rpt
DDILLKEELDSW NIA1_TOBAC 815 18 -
DDILLKEELDSW NIA2_TOBAC 815 18 -
DDILLREELDTW NIA_LOTJA 812 18 -
DDILLKEELDAW O24390 822 18 -
DDILLKEELDAW O04926 822 18 -
DDILLKDELDSW NIA_PETHY 820 18 -
DDILLKDELDSW Q43042 826 18 -
DDILLKDELDAW NIA_LYCES 822 18 -
DDILLRDELDTW NIA_CUCMA 830 18 -
DDILLREELDTW NIA2_PHAVU 802 18 -
EDILLREELDKW NIA_SPIOL 838 18 -
DDILLKEELDEW NIA2_SOYBN 802 18 -
DDILVREELEGW NIA1_BRANA 822 18 -
DDILLKEELDEW NIA1_SOYBN 798 18 -
DDILLKEELDEW NIA1_PHAVU 793 18 -
DDILLRDELDRW NIA1_ORYSA 825 19 -
DDILVREELEGW NIA2_BRANA 822 18 -
DDILLRAELDRW NIA3_MAIZE 799 19 -
DDILLRDELDRW NIA1_HORVU 824 19 -
DDILLREELDDW NIA_BETVE 810 18 -
DDILVREELEGW NIA1_ARATH 828 18 -
EDILLREELDGW NIA2_ARATH 828 18 -
DDILLREELDAW NIA_CICIN 831 18 -
DDMLLREEIDRW NIA7_HORVU 799 19 -
EDILLREELDEL NIA_VOLCA 775 18 -
DDILLREELDAL Q42497 791 18 -
KDILLRPELEEL NC5R_HUMAN 213 18 -
DDILLREELDHI NIA_PICAN 769 18 -
KDILLRPELEEL NC5R_BOVIN 213 18 -
EDILCRAELDGF Q12561 783 18 -
KDILLRPELEEL NC5R_RAT 213 18 -
DDILCRQELDDL O16521 222 18 -
EDILCREDLDRF NIA_LEPMC 808 18 -
EDILLKNELDEF NIA_EMENI 785 18 -
EDILCREELDDL Q00303 809 20 -
DDILCKEELDQL Q92237 817 18 -
DDILCRKELDEL O16522 216 18 -
EDILCRLELDQM O13486 804 18 -
EDILCRSDLDAY Q01696 779 18 -
EDILCRADLDAY NIA_ASPNG 782 18 -
NDILCMEELEEL NIA_FUSOX 815 18 -
KDILCREELDAM NIA_BEABA 805 18 -
EDILCREDLDVF O00101 804 18 -
QDILIREELDAL NIA_USTMA 809 28 -
RDLLLRKELETL NIA_PHYIN 807 18 -
EDILLKKELEAL NC5R_YEAST 226 18 -
GDILMKSELDEL NIA_NEUCR 892 18 -
GDIVLRDEIDAL O74557 208 18 -

Motif 6 width=9
Element Seqn Id St Int Rpt
LALACGPPP NIA1_TOBAC 872 45 -
LALACGPPP NIA2_TOBAC 872 45 -
LALACGPPP NIA_LOTJA 868 44 -
LALACGPPP O24390 879 45 -
LALACGPPP O04926 879 45 -
LALACGPPP NIA_PETHY 877 45 -
LALACGPPP Q43042 883 45 -
LALACGPPP NIA_LYCES 879 45 -
LALACGPPA NIA_CUCMA 886 44 -
LAMTCGPPP NIA2_PHAVU 858 44 -
LALTCGPPP NIA_SPIOL 894 44 -
LALTCGPPP NIA2_SOYBN 858 44 -
LALACGPPP NIA1_BRANA 879 45 -
LALTCGPPP NIA1_SOYBN 854 44 -
LALTCGPPP NIA1_PHAVU 849 44 -
LALACGPPP NIA1_ORYSA 884 47 -
LALACGPPP NIA2_BRANA 879 45 -
LALLCGPPS NIA3_MAIZE 857 46 -
LALACGPPP NIA1_HORVU 883 47 -
LALACGAPS NIA_BETVE 866 44 -
LALACGPPP NIA1_ARATH 885 45 -
LAMACGPPP NIA2_ARATH 885 45 -
LALACGPPP NIA_CICIN 888 45 -
LALVCGPPA NIA7_HORVU 859 48 -
LALMCGPPA NIA_VOLCA 833 46 -
ICCLCGPPP Q42497 846 43 -
LVLMCGPPP NC5R_HUMAN 269 44 -
MLLVCGPPG NIA_PICAN 829 48 -
LVLMCGPPP NC5R_BOVIN 269 44 -
MVLICGPPA Q12561 838 43 -
LILMCGPPP NC5R_RAT 269 44 -
AVLLCGPPP O16521 278 44 -
LVLVCGPEG NIA_LEPMC 863 43 -
MVLVCGPEA NIA_EMENI 843 46 -
LVLICGPEA Q00303 877 56 -
LVLVCGPPP Q92237 877 48 -
AVLLCGPPA O16522 272 44 -
IVLVCGPEQ O13486 862 46 -
MVLVCGPGP Q01696 834 43 -
MVLICGPEA NIA_ASPNG 837 43 -
LLLVCGPPP NIA_FUSOX 875 48 -
MVLVCGPAA NIA_BEABA 864 47 -
LVLICGPEA O00101 861 45 -
LALVCGPPP NIA_USTMA 877 56 -
DVCMCGPEG NIA_PHYIN 870 51 -
QILICGPPA NC5R_YEAST 284 46 -
MVLLCGPEG NIA_NEUCR 952 48 -
KVLICGPTP O74557 263 43 -