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PR00369

Identifier
FLAVODOXIN  [View Relations]  [View Alignment]  
Accession
PR00369
No. of Motifs
4
Creation Date
03-MAR-1995  (UPDATE 24-JUN-1999)
Title
Flavodoxin signature
Database References

PROSITE; PS00201 FLAVODOXIN
INTERPRO; IPR001094
PDB; 2FX2
SCOP; 2FX2
CATH; 2FX2
Literature References
1. VERVOORT, J., HEERING, D., PEELEN, S. AND VAN BERKEL, W.
Flavodoxins.
METHODS ENZYMOL. 243 188-203 (1994).
 
2. WAKABAYASHI, S., KIMURA, T., FUKUYAMA, K., MATSUBARA, H. AND 
ROGERS, L.J.
The amino acid sequence of a flavodoxin from the eukaryotic red alga 
Chondrus crispus.
BIOCHEM.J. 263 981-984 (1989).
 
3. PORTER, T.D. AND KASPER, C.B.
NADPH-cytochrome P-450 oxidoreductase: flavin mononucleotide and 
flavin adenine dinucleotide domains evolved from different flavoproteins.
BIOCHEMISTRY 25 1682-1687 (1986).
 
4. OSTROWSKI, J., BARBER, M.J., RUEGER, D.C., MILLER, B.E., SIEGEL, L.M.
AND KREDICH, N.M.
Characterization of the flavoprotein moieties of NADPH-sulfite reductase 
from Salmonella typhimurium and Escherichia coli. Physicochemical and 
catalytic properties, amino acid sequence deduced from DNA sequence 
of cysJ, and comparison with NADPH-cytochrome P-450 reductase.
J.BIOL.CHEM. 264 15796-15808 (1989).
 
5. BREDT, D.S., HWANG, P.M., GLATT, C.E., LOWENSTEIN, C., REED, R.R. 
AND SNYDER, S.H.
Cloned and expressed nitric oxide synthase structurally resembles 
cytochrome P-450 reductase. 
NATURE 351 714-718 (1991). 
 
6. RAO, S.T., SHAFFIE, F., YU, C., SATYSHUR, K.A., STOCKMAN, B.J.,
MARKLEY, J.L. AND SUNDARALINGAM, M.
Structure of the oxidized long-chain flavodoxin from Anabaena 7120
at 2 A resolution.
PROTEIN SCI. 1 1413-1427 (1992).

Documentation
Flavodoxins act in various electron-transport systems as functional
analogues of ferredoxins [1]. Although flavodoxins are found only in 
certain bacteria and algae [2], the proteins share similarity with a 
number of protein domains of both prokaryotic and eukaryotic origin, 
in particular to the FMN-binding domain in NADPH:P450 reductases [3]. 
The flavoprotein component of bacterial NADPH-sulfite reductase [4] and 
the C-terminal domain of mammalian nitric oxide synthases (NOS) [5]
show pronounced sequence similarity to NADPH:P450 reductases.
 
The 3D-structures of several flavodoxins from bacteria and algae have been 
solved. All of them have a doubly-wound alternating alpha/beta fold 
consisting of a five-stranded parallel beta-sheet core with a pair of 
flanking alpha-helices [6]. FMN binds at one end of the sheet, with its
dimethyl benzene edge exposed to solvent.  
 
FLAVODOXIN is a 4-element fingerprint that provides a signature for
flavodoxins. The fingerprint was derived from an initial alignment of 24
sequences: motif 1 spans the N-terminal region encoded by PROSITE pattern
FLAVODOXIN (PS00201), which is involved in binding the FMN phosphate group
- within the 3D-structure of bacterial flavodoxin (2FX2), this motif
corresponds to the loop between beta-strand 2 and helix 1; motif 2 spans
part of beta-strand 3 and the adjacent beta-turn; motif 3 coincides with
beta-strand 4; and motif 4 includes helix 3. Five iterations on OWL25.1 
were required to reach convergence, at which point a true set comprising
66 sequences was identified. Several partial matches were also found,
including various flavodoxins, a bacterial P450 reductase, E.coli MIOC
protein, and a number of hypothetical proteins.
 
An update on SPTR37_9f identified a true set of 64 sequences, and 36
partial matches.
Summary Information
  64 codes involving  4 elements
20 codes involving 3 elements
5 codes involving 2 elements
Composite Feature Index
464646464
31916205
24411
1234
True Positives
CYSJ_ECOLI    CYSJ_SALTY    CYSJ_THIRO    FLAV_AZOVI    
FLAV_DESGI FLAV_DESSA FLAV_DESVH FLAV_DESVM
FLAV_SYNY3 FLAW_ECOLI NCPR_CANMA NCPR_CANTR
NCPR_CATRO NCPR_CAVPO NCPR_HUMAN NCPR_MOUSE
NCPR_MUSDO NCPR_PHAAU NCPR_PIG NCPR_RABIT
NCPR_RAT NCPR_SCHPO NCPR_YEAST NOS1_HUMAN
NOS1_RAT NOS2_CHICK NOS2_HUMAN NOS2_MOUSE
NOS2_RAT NOS3_BOVIN NOS3_HUMAN O14167
O19132 O24424 O24425 O32214
O48937 O48938 O54705 O60471
O60591 O60604 O60757 O61309
O61608 O70515 O70516 O77104
P70313 P70594 P91655 Q00141
Q09590 Q12181 Q17574 Q26240
Q27571 Q27597 Q28969 Q39035
Q39036 Q43235 Q60451 YJ9F_YEAST
True Positive Partials
Codes involving 3 elements
CPXB_BACME FLAV_ANASP FLAV_AZOCH FLAV_BACSU
FLAV_ECOLI FLAV_ENTAG FLAV_HAEIN FLAV_RHOCA
FLAV_SYNP2 FLAV_SYNP7 FLAW_BACSU FLAW_DESGI
FLAW_ENTAG FLAW_KLEPN MIOC_HAEIN O08336
O08394 O59761 O68591 Q46917
Codes involving 2 elements
FLAV_CHOCR FLAV_HELPY MIOC_ECOLI O68226
Q47418
Sequence Titles
CYSJ_ECOLI  SULFITE REDUCTASE [NADPH] FLAVOPROTEIN ALPHA-COMPONENT (EC 1.8.1.2) (SIR-FP) - ESCHERICHIA COLI. 
CYSJ_SALTY SULFITE REDUCTASE [NADPH] FLAVOPROTEIN ALPHA-COMPONENT (EC 1.8.1.2) (SIR-FP) - SALMONELLA TYPHIMURIUM.
CYSJ_THIRO SULFITE REDUCTASE [NADPH] FLAVOPROTEIN ALPHA-COMPONENT (EC 1.8.1.2) (SIR-FP) - THIOCAPSA ROSEOPERSICINA.
FLAV_AZOVI FLAVODOXIN 2 - AZOTOBACTER VINELANDII.
FLAV_DESGI FLAVODOXIN - DESULFOVIBRIO GIGAS.
FLAV_DESSA FLAVODOXIN - DESULFOVIBRIO SALEXIGENS.
FLAV_DESVH FLAVODOXIN - DESULFOVIBRIO VULGARIS (STRAIN HILDENBOROUGH).
FLAV_DESVM FLAVODOXIN - DESULFOVIBRIO VULGARIS (STRAIN MIYAZAKI).
FLAV_SYNY3 FLAVODOXIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
FLAW_ECOLI FLAVODOXIN 2 - ESCHERICHIA COLI.
NCPR_CANMA NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - CANDIDA MALTOSA (YEAST).
NCPR_CANTR NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - CANDIDA TROPICALIS (YEAST).
NCPR_CATRO NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) - CATHARANTHUS ROSEUS (ROSY PERIWINKLE) (MADAGASCAR PERIWINKLE).
NCPR_CAVPO NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - CAVIA PORCELLUS (GUINEA PIG).
NCPR_HUMAN NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - HOMO SAPIENS (HUMAN).
NCPR_MOUSE NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - MUS MUSCULUS (MOUSE).
NCPR_MUSDO NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) - MUSCA DOMESTICA (HOUSE FLY).
NCPR_PHAAU NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - PHASEOLUS AUREUS (MUNG BEAN) (VIGNA RADIATA).
NCPR_PIG NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - SUS SCROFA (PIG).
NCPR_RABIT NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - ORYCTOLAGUS CUNICULUS (RABBIT).
NCPR_RAT NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - RATTUS NORVEGICUS (RAT).
NCPR_SCHPO NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
NCPR_YEAST NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
NOS1_HUMAN NITRIC-OXIDE SYNTHASE, BRAIN (EC 1.14.13.39) (NOS, TYPE I) (NEURONAL NOS) (NNOS) - HOMO SAPIENS (HUMAN).
NOS1_RAT NITRIC-OXIDE SYNTHASE, BRAIN (EC 1.14.13.39) (NOS, TYPE I) (NEURONAL NOS) (NNOS) - RATTUS NORVEGICUS (RAT).
NOS2_CHICK NITRIC OXIDE SYNTHASE, INDUCIBLE (EC 1.14.13.39) (NOS, TYPE II) (INDUCIBLE NOS) (INOS) (MACROPHAGE NOS) - GALLUS GALLUS (CHICKEN).
NOS2_HUMAN NITRIC OXIDE SYNTHASE, INDUCIBLE (EC 1.14.13.39) (NOS, TYPE II) (INDUCIBLE NOS) (INOS) (HEPATHOCYTE NOS) (HEP-NOS) - HOMO SAPIENS (HUMAN).
NOS2_MOUSE NITRIC OXIDE SYNTHASE, INDUCIBLE (EC 1.14.13.39) (NOS, TYPE II) (INDUCIBLE NOS) (INOS) (MACROPHAGE NOS) (MAC-NOS) - MUS MUSCULUS (MOUSE).
NOS2_RAT NITRIC OXIDE SYNTHASE, INDUCIBLE (EC 1.14.13.39) (NOS, TYPE II) (INDUCIBLE NOS) (INOS) - RATTUS NORVEGICUS (RAT).
NOS3_BOVIN NITRIC-OXIDE SYNTHASE, ENDOTHELIAL (EC 1.14.13.39) (EC-NOS) (NOS, TYPE III) (ENDOTHELIAL NOS) (ENOS) - BOS TAURUS (BOVINE).
NOS3_HUMAN NITRIC-OXIDE SYNTHASE, ENDOTHELIAL (EC 1.14.13.39) (EC-NOS) (NOS, TYPE III) (ENDOTHELIAL NOS) (ENOS) - HOMO SAPIENS (HUMAN).
O14167 PUTATIVE SULFITE REDUCTASE (FERREDOXIN) (EC 1.8.7.1) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O19132 NITRIC-OXIDE SYNTHASE, BRAIN (EC 1.14.13.39) (NOS, TYPE I) (NEURONAL NOS) (NNOS) (CONSTITUTIVE NOS) (NC-NOS) (BNOS) - ORYCTOLAGUS CUNICULUS (RABBIT).
O24424 NADPH:FERRIHEMOPROTEIN OXIDOREDUCTASE (EC 1.6.2.4) - PAPAVER SOMNIFERUM (OPIUM POPPY).
O24425 NADPH:FERRIHEMOPROTEIN OXIDOREDUCTASE (EC 1.6.2.4) - ESCHSCHOLZIA CALIFORNICA (CALIFORNIA POPPY).
O32214 YVGR PROTEIN - BACILLUS SUBTILIS.
O48937 NADPH CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (NADPH--FERRIHEMOPROTEIN REDUCTASE) (NADPH--CYTOCHROME P450 REDUCTASE) (TPNH(2) CYTOCHROME C REDUCTASE) (FERRIHEMOPROTEIN P450 REDUCTASE) - PETROSELINUM CRISPUM (PARSLEY) (PETROSELINUM HORTENSE).
O48938 NADPH CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (NADPH--FERRIHEMOPROTEIN REDUCTASE) (NADPH--CYTOCHROME P450 REDUCTASE) (TPNH(2) CYTOCHROME C REDUCTASE) (FERRIHEMOPROTEIN P450 REDUCTASE) - PETROSELINUM CRISPUM (PARSLEY) (PETROSELINUM HORTENSE).
O54705 NITRIC OXIDE SYNTHASE, INDUCIBLE (EC 1.14.13.39) (NOS, TYPE II) (INDUCIBLE NOS) (INOS) - CAVIA PORCELLUS (GUINEA PIG).
O60471 METHIONINE SYNTHASE REDUCTASE - HOMO SAPIENS (HUMAN).
O60591 NITRIC OXIDE SYNTHASE, INDUCIBLE (EC 1.14.13.39) (NOS, TYPE II) (INDUCIBLE NOS) (INOS) - HOMO SAPIENS (HUMAN).
O60604 NITRIC OXIDE SYNTHASE, INDUCIBLE (EC 1.14.13.39) (NOS, TYPE II) (INDUCIBLE NOS) (INOS) - HOMO SAPIENS (HUMAN).
O60757 INDUCIBLE NITRIC OXIDE SYNTHASE - HOMO SAPIENS (HUMAN).
O61309 NITRIC-OXIDE SYNTHASE (EC 1.14.13.39) (NOS, TYPE I) (NEURONAL NOS) (NNOS) - LYMNAEA STAGNALIS (GREAT POND SNAIL).
O61608 NITRIC-OXIDE SYNTHASE (EC 1.14.13.39) - ANOPHELES STEPHENSI (INDO-PAKISTAN MALARIA MOSQUITO).
O70515 NITRIC OXIDE SYNTHASE 2 - MUS MUSCULUS (MOUSE).
O70516 NITRIC OXIDE SYNTHASE 2 - MUS MUSCULUS (MOUSE).
O77104 NITRIC OXIDE SYNTHASE - MANDUCA SEXTA (TOBACCO HAWKMOTH) (TOBACCO HORNWORM).
P70313 NITRIC-OXIDE SYNTHASE, ENDOTHELIAL (EC 1.14.13.39) (EC-NOS) (NOS, TYPE III) (ENDOTHELIAL NOS) (ENOS) (CONSTITUTIVE NOS) (CNOS) - MUS MUSCULUS (MOUSE).
P70594 NEURONAL NITRIC OXIDE SYNTHASE - RATTUS NORVEGICUS (RAT).
P91655 NADPH-CYTOCHROME P450 REDUCTASE - DROSOPHILA METTLERI (FRUIT FLY).
Q00141 NADPH CYTOCHROME P450 OXIDOREDUCTASE - ASPERGILLUS NIGER.
Q09590 PROBABLE NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4) (CPR) - CAENORHABDITIS ELEGANS.
Q12181 HYPOTHETICAL 72.3 KD PROTEIN YPR044W - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
Q17574 C01G6.6 PROTEIN - CAENORHABDITIS ELEGANS.
Q26240 NITRIC-OXIDE SYNTHASE (EC 1.14.13.39) - RHODNIUS PROLIXUS.
Q27571 NITRIC-OXIDE SYNTHASE (EC 1.14.13.39) (DNOS) - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q27597 NADPH--FERRIHEMOPROTEIN REDUCTASE (EC 1.6.2.4) (NADPH--CYTOCHROME P450 REDUCTASE) (TPNH(2) CYTOCHROME C REDUCTASE) (FERRIHEMOPROTEIN P450 REDUCTASE) - DROSOPHILA MELANOGASTER (FRUIT FLY).
Q28969 NITRIC-OXIDE SYNTHASE, ENDOTHELIAL (EC 1.14.13.39) (EC-NOS) (NOS, TYPE III) (ENDOTHELIAL NOS) (ENOS) (CONSTITUTIVE NOS) (CNOS) - SUS SCROFA (PIG).
Q39035 NADPH-FERRIHEMOPROTEIN REDUCTASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q39036 NADPH-FERRIHEMOPROTEIN REDUCTASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q43235 NADPH-FERRIHEMOPROTEIN REDUCTASE (EC 1.6.2.4) (NADPH--FERRIHEMOPROTEIN REDUCTASE) (NADPH--CYTOCHROME P450 REDUCTASE) (TPNH(2) CYTOCHROME C REDUCTASE) (FERRIHEMOPROTEIN P450 REDUCTASE) - VICIA SATIVA (SPRING VETCH) (TARE).
Q60451 NADPH-CYTOCHROME P450 OXIDOREDUCTASE - CRICETULUS GRISEUS (CHINESE HAMSTER).
YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN IN NMD5-HOM6 INTERGENIC REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).

CPXB_BACME CYTOCHROME P450(BM-3) (P450BM-3) [INCLUDES: CYTOCHROME P450 102 (EC 1.14.14.1); NADPH-CYTOCHROME P450 REDUCTASE (EC 1.6.2.4)] - BACILLUS MEGATERIUM.
FLAV_ANASP FLAVODOXIN - ANABAENA SP. (STRAIN PCC 7120), AND ANABAENA SP. (STRAIN PCC 7119).
FLAV_AZOCH FLAVODOXIN B (FLDB) - AZOTOBACTER CHROOCOCCUM MCD 1.
FLAV_BACSU PROBABLE FLAVODOXIN 1 - BACILLUS SUBTILIS.
FLAV_ECOLI FLAVODOXIN 1 - ESCHERICHIA COLI.
FLAV_ENTAG FLAVODOXIN - ENTEROBACTER AGGLOMERANS.
FLAV_HAEIN FLAVODOXIN - HAEMOPHILUS INFLUENZAE.
FLAV_RHOCA FLAVODOXIN 2 - RHODOBACTER CAPSULATUS (RHODOPSEUDOMONAS CAPSULATA).
FLAV_SYNP2 FLAVODOXIN - SYNECHOCOCCUS SP. (STRAIN PCC 7002) (AGMENELLUM QUADRUPLICATUM).
FLAV_SYNP7 FLAVODOXIN - SYNECHOCOCCUS SP. (STRAIN PCC 7942) (ANACYSTIS NIDULANS R2).
FLAW_BACSU PROBABLE FLAVODOXIN 2 - BACILLUS SUBTILIS.
FLAW_DESGI FLAVODOXIN - DESULFOVIBRIO GIGAS.
FLAW_ENTAG FLAVODOXIN - ENTEROBACTER AGGLOMERANS.
FLAW_KLEPN FLAVODOXIN - KLEBSIELLA PNEUMONIAE.
MIOC_HAEIN MIOC PROTEIN HOMOLOG - HAEMOPHILUS INFLUENZAE.
O08336 CYTOCHROME P450 102 (EC 1.14.14.1) - BACILLUS SUBTILIS.
O08394 PROBABLE CYTOCHROME P450 (YFNJ) (EC 1.14.14.1) - BACILLUS SUBTILIS.
O59761 CONSERVED HYPOTHETICAL PROTEIN - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O68591 IRON-UPTAKE FACTOR - PSEUDOMONAS AERUGINOSA.
Q46917 CORRESPONDS TO HYPOTHETICAL PROTEIN FROM E. CAROTOVORA PIR ACCESSION NUMBER S45107 - ESCHERICHIA COLI.

FLAV_CHOCR FLAVODOXIN - CHONDRUS CRISPUS (CARRAGHEEN).
FLAV_HELPY FLAVODOXIN - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
MIOC_ECOLI MIOC PROTEIN - ESCHERICHIA COLI.
O68226 FLAVODOXIN A - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
Q47418 (SCRI193) - ERWINIA CAROTOVORA.
Scan History
OWL25_1    5  250  NSINGLE    
SPTR37_9f 6 500 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
GLFYGTQTGNTETI FLAV_SYNY3 5 5 -
GLFFGSNTGKTRKV FLAV_AZOVI 4 4 -
LIVYGSTTGNTEGV FLAV_DESGI 5 5 -
LVLYASQTGTAEDY NCPR_YEAST 61 61 -
AVFFGSQTGTAEDF NCPR_SCHPO 54 54 -
TIFFGTQTGTAEGF NCPR_PHAAU 83 83 -
TILYATETGKSQAY NOS1_RAT 756 756 -
TILFATETGKSEAL NOS2_HUMAN 540 540 -
TILYASETGRAQSY NOS3_BOVIN 522 522 -
VVFYGSQTGTAEEF NCPR_RABIT 82 82 -
IVFYGSQTGTAEEF NCPR_PIG 80 80 -
VVFYGSQTGTAEEF NCPR_MUSDO 78 78 -
TIFFGTQTGTAEGF HTU2NFR 93 93 -
VVFYGSQTGTGEEF NCPR_SALTR 26 26 -
LVLYGSNMGTAEGT CPXB_BACME 483 483 -
GIFFGSDTGNTENI FLAV_ECOLI 4 4 -
GLFFGTQTGNTEEL FLAV_SYNP2 4 4 -
GLFYGTQTGKTESV FLAV_ANASP 5 5 -
GLFFGSNTGKTRKV FLAV_AZOCH 4 4 -
GIFFGTDTGKTRKI O07026 4 4 -
GIFFGSDTGQTRKV FLAV_ENTAG 5 5 -
GLFYGTQTGVTQTI FLAV_SYNP7 4 4 -
EIVYWSGTGNTEAM P00321 3 3 -
LIVFGSSTGNTESI P26492 5 5 -

Motif 2 width=12
Element Seqn Id St Int Rpt
FFFLATYGDGEP HTU2NFR 149 42 -
IFCMATYGEGDP NCPR_SALTR 79 39 -
VTASYNGHPPDN CPXB_BACME 532 35 -
LLGIPTWYYGEA FLAV_ECOLI 50 32 -
IIGCPTWNVGEL FLAV_SYNP2 51 33 -
IIGCPTWNIGEL FLAV_ANASP 51 32 -
ILGTPTLGEGEL FLAV_AZOCH 51 33 -
LLGTPTLGDGQL O07026 50 32 -
LLGTPTLGDGEL FLAV_ENTAG 51 32 -
IIGCPTWNVGEL FLAV_SYNP7 51 33 -
LLGCPAMGSEEL P00321 51 34 -
LFGCSAWGMEDL P26492 54 35 -
VFCLATYGEGDP NCPR_MUSDO 131 39 -
VFCMATYGEGDP NCPR_PIG 133 39 -
VFCMATYGEGDP NCPR_RABIT 135 39 -
LVVTSTFGNGDP NOS3_BOVIN 569 33 -
LVVTSTFGNGDC NOS2_HUMAN 587 33 -
LVVTSTFGNGDP NOS1_RAT 803 33 -
FFMLATYGDGEP NCPR_PHAAU 139 42 -
VFFLATYGEGEP NCPR_SCHPO 106 38 -
SIFISTYGEGDF NCPR_YEAST 111 36 -
LLGCSTWGDDEI FLAV_DESGI 54 35 -
ILGTPTLGEGEL FLAV_AZOVI 51 33 -
IIGCPTWNVGEL FLAV_SYNY3 52 33 -

Motif 3 width=11
Element Seqn Id St Int Rpt
GRKVAAFASGD P26492 85 19 -
LFGSYGWGSGE P00321 85 22 -
GKKVAYFGAGD FLAV_SYNP7 80 17 -
GKTVALFGLGD FLAV_ENTAG 88 25 -
GKTVALFGLGD O07026 87 25 -
GKTVALFGLGD FLAV_AZOCH 88 25 -
GKLVAYFGTGD FLAV_ANASP 80 17 -
GKTIAYFGAGD FLAV_SYNP2 80 17 -
GKLVALFGCGD FLAV_ECOLI 79 17 -
GVRYSVFGCGD CPXB_BACME 561 17 -
GVNYPVFALGD NCPR_SALTR 110 19 -
KLHYGVFGLGN HTU2NFR 183 22 -
GLNYAVFGLGN NCPR_MUSDO 162 19 -
GVKYAVFGLGN NCPR_PIG 164 19 -
GVKYAVFGLGN NCPR_RABIT 166 19 -
TLRFCVFGLGS NOS3_BOVIN 645 64 -
KFRYAVFGLGS NOS2_HUMAN 618 19 -
NVRFSVFGLGS NOS1_RAT 876 61 -
KLTYGVFGLGN NCPR_PHAAU 174 23 -
GIRYAIFGLGN NCPR_SCHPO 148 30 -
NLRYNMFGLGN NCPR_YEAST 143 20 -
DKKVGVFGCGD FLAV_DESGI 85 19 -
GKTVALFGLGD FLAV_AZOVI 88 25 -
GKKVAYFGAGD FLAV_SYNY3 81 17 -

Motif 4 width=20
Element Seqn Id St Int Rpt
DEGLTEQGAKRFVPVGLGDD HTU2NFR 207 13 -
DKRLEELGAKRVFDLGMGDD NCPR_SALTR 134 13 -
DETLAAKGAENIADRGEADA CPXB_BACME 586 14 -
EYFCDALGTIRDIIEPRGAT FLAV_ECOLI 95 5 -
DNFQDAMGVLEEKITSLGGK FLAV_SYNP2 96 5 -
DNFQDAIGILEEKISQRGGK FLAV_ANASP 96 5 -
ENFLDAMGELHSFFTERGAK FLAV_AZOCH 104 5 -
DNFVSGMRPLFDALSARGAQ O07026 103 5 -
KNFVSAMRILYDLVIARGAC FLAV_ENTAG 104 5 -
DNFQDAMGILEEKISSLGSQ FLAV_SYNP7 96 5 -
WMDAWKQRTEDTGATVIGTA P00321 96 0 -
EHFCGAVPAIEERAKELGAT P26492 99 3 -
DKRLEELGATRVFELGLGDD NCPR_MUSDO 186 13 -
DKRLEQLGAQRIFDLGLGDD NCPR_PIG 188 13 -
DQRLEQLGAQRIFELGMGDD NCPR_RABIT 190 13 -
DTRLEELGGERLLQLGQGDE NOS3_BOVIN 669 13 -
DQKLSHLGASQLTPMGEGDE NOS2_HUMAN 642 13 -
DTLLEELGGERILKMREGDE NOS1_RAT 900 13 -
DEELAEQGAKRLVAVGLGDD NCPR_PHAAU 198 13 -
DAAMTRLGATRVGNLGLGDD NCPR_SCHPO 172 13 -
EKHLSAAGAIRLGKLGEADD NCPR_YEAST 167 13 -
TYFCGAVDVIEKKAEELGAT FLAV_DESGI 99 3 -
ENYLDALGELYSFFKDRGAK FLAV_AZOVI 104 5 -
DNFQDAMGILEEKISGLGGK FLAV_SYNY3 97 5 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
VVFYGSQTGTAEEF NCPR_MUSDO 78 78 -
IVFYGSQTGTAEEF NCPR_CAVPO 80 80 -
IVFYGSQTGTAEEF NCPR_PIG 80 80 -
IVFYGSQTGTAEEF NCPR_MOUSE 80 80 -
IVFYGSQTGTAEEF Q60451 70 70 -
IVFYGSQTGTAEEF NCPR_RAT 80 80 -
IVFYGSQTGTAEEF NCPR_HUMAN 80 80 -
VVFYGSQTGTAEEF NCPR_RABIT 82 82 -
VVFYGSQTGTGEEF Q27597 85 85 -
VVFYGSQTGTGEEF P91655 86 86 -
TILYGSETGRAQSY NOS3_HUMAN 520 520 -
TILYGSETGRAQSY P70313 520 520 -
TILYASETGRAQSY NOS3_BOVIN 522 522 -
TVLFATETGKSEAL NOS2_MOUSE 534 534 -
TVLFATETGKSEAL O70516 186 186 -
TVLFATETGKSEAL O70515 534 534 -
TVLFATETGKSEAL O60604 537 537 -
TVLFATETGKSEAL NOS2_RAT 537 537 -
TILFATETGKSEAL O54705 539 539 -
TILFATETGKSEAL NOS2_HUMAN 540 540 -
TILFATETGKSEAL O60757 540 540 -
LIMYGSQTGTAEEM Q09590 74 74 -
TVLFATETGKSEAL O60591 537 537 -
TIFFGTQTGTAEGF Q39036 106 106 -
TIFYGTQTGTAEGF O48938 92 92 -
TIFFGTQTGTAEGF O24425 96 96 -
TILYATETGKSQAY P70594 756 756 -
TILYATETGKSQAY O19132 762 762 -
TILYATETGKSQAY NOS1_RAT 756 756 -
TILYATETGKSQAY NOS1_HUMAN 761 761 -
TVIYATETGKSETL NOS2_CHICK 537 537 -
TIFFGTQTGTAEGF NCPR_PHAAU 83 83 -
TIFFGTQTGTAEGF O24424 81 81 -
TILFATETGKSEMY Q26240 506 506 -
SILYASETVRAQSY Q28969 523 523 -
TVFFGTQTGTAEGF O48937 75 75 -
LLLFGSQTGTAEDY NCPR_CANMA 61 61 -
TIFFGTQTGTAEGF NCPR_CATRO 105 105 -
TVFYGTQTGTAEGF Q43235 84 84 -
TVLYATETGRSEQY O61608 568 568 -
TVLYATETGKSEQY Q27571 673 673 -
TILYATETGKSEQY O77104 520 520 -
LLLFGSQTGTAEDY NCPR_CANTR 61 61 -
AVFFGSQTGTAEDF NCPR_SCHPO 54 54 -
SIFYATETGRSERF O61309 428 428 -
SIFFGTQTGTAEGF Q39035 86 86 -
VIFYGSQTGTAEDY Q00141 67 67 -
TILFGSQTGNAKAV CYSJ_THIRO 61 61 -
LVLYASQTGTAEDY NCPR_YEAST 61 61 -
TVLYGSQTGNAQGL O32214 69 69 -
TIISASQTGNARRV CYSJ_ECOLI 64 64 -
TLISASQTGNARRV CYSJ_SALTY 64 64 -
TILFASDGGTAENV O14167 729 729 -
HVYYASDGGNAANL YJ9F_YEAST 683 683 -
LLLYATQQGQAKAI O60471 5 5 -
LIAFGSQTGQAETI Q17574 5 5 -
LIVYGSTTGNTETA FLAV_DESSA 5 5 -
LIVYGSTTGNTEGV FLAV_DESGI 5 5 -
VILYGSETGNAHDF Q12181 8 8 -
GLFFGSNTGKTRKV FLAV_AZOVI 4 4 -
LIVYGSTTGNTAWV FLAV_DESVM 5 5 -
GLFYGTQTGNTETI FLAV_SYNY3 5 5 -
LIVYGSTTGNTEYT FLAV_DESVH 5 5 -
GLFYGSSTCYTEMA FLAW_ECOLI 4 4 -

Motif 2 width=12
Element Seqn Id St Int Rpt
VFCLATYGEGDP NCPR_MUSDO 131 39 -
VFCMATYGEGDP NCPR_CAVPO 133 39 -
VFCMATYGEGDP NCPR_PIG 133 39 -
VFCMATYGEGDP NCPR_MOUSE 133 39 -
VFCMATYGEGDP Q60451 123 39 -
VFCMATYGEGDP NCPR_RAT 133 39 -
VFCMATYGEGDP NCPR_HUMAN 133 39 -
VFCMATYGEGDP NCPR_RABIT 135 39 -
VFCLATYGEGDP Q27597 138 39 -
VFCLATYGEGDP P91655 139 39 -
LVVTSTFGNGDP NOS3_HUMAN 567 33 -
LVVTSTFGNGDP P70313 567 33 -
LVVTSTFGNGDP NOS3_BOVIN 569 33 -
LVVTSTFGNGDC NOS2_MOUSE 581 33 -
LVVTSTFGNGDC O70516 233 33 -
LVVTSTFGNGDC O70515 581 33 -
LVVTSTFGNGDC O60604 584 33 -
LVVTSTFGNGDC NOS2_RAT 584 33 -
LVVTSTFGNGDC O54705 586 33 -
LVVTSTFGNGDC NOS2_HUMAN 587 33 -
LVVTSTFGNGDC O60757 587 33 -
VLCIATYGEGDP Q09590 127 39 -
LVVTSTFGNGDC O60591 584 33 -
FFFLATYGDGEP Q39036 162 42 -
FFFLATYGDGEP O48938 148 42 -
LFFLATYGDGEP O24425 152 42 -
LVVTSTFGNGDP P70594 803 33 -
LVVTSTFGNGDP O19132 809 33 -
LVVTSTFGNGDP NOS1_RAT 803 33 -
LVVTSTFGNGDP NOS1_HUMAN 808 33 -
LVVTSTFGNGDS NOS2_CHICK 584 33 -
FFMLATYGDGEP NCPR_PHAAU 139 42 -
FFMVATYGDGEP O24424 137 42 -
LVVASTFGNGDP Q26240 553 33 -
LVVTSTFGNGDP Q28969 570 33 -
FFFLATYGDGEP O48937 131 42 -
FFIVATYGEGEP NCPR_CANMA 113 38 -
FFILATYGDGEP NCPR_CATRO 161 42 -
FFMLATYGDGEP Q43235 140 42 -
LVVASTFGNGDP O61608 615 33 -
IVVASTFGNGDP Q27571 720 33 -
LIVTSTFGNGEP O77104 567 33 -
FFIVATYGEGEP NCPR_CANTR 113 38 -
VFFLATYGEGEP NCPR_SCHPO 106 38 -
MVITSTFGNGEP O61309 475 33 -
FFCVATYGDGEP Q39035 142 42 -
FFVLATYGEGEP Q00141 119 38 -
LIVVSTHGEGEP CYSJ_THIRO 109 34 -
SIFISTYGEGDF NCPR_YEAST 111 36 -
LIVVSTHGEGEP O32214 117 34 -
IVVTSTQGEGEP CYSJ_ECOLI 112 34 -
VIVTSTQGEGEP CYSJ_SALTY 112 34 -
VVLVSTAGQGEF O14167 777 34 -
VFITSTAGQGEF YJ9F_YEAST 731 34 -
VVVVSTTGTGDP O60471 54 35 -
AIVVSSTGDGDA Q17574 54 35 -
LFGCSTWGEEEI FLAV_DESSA 54 35 -
LLGCSTWGDDEI FLAV_DESGI 54 35 -
FIICSTTGQGEL Q12181 56 34 -
ILGTPTLGEGEL FLAV_AZOVI 51 33 -
LFGCSTWGDDEI FLAV_DESVM 54 35 -
IIGCPTWNVGEL FLAV_SYNY3 52 33 -
LLGCSTWGDDSI FLAV_DESVH 54 35 -
ILGIPTWDFGEI FLAW_ECOLI 50 32 -

Motif 3 width=11
Element Seqn Id St Int Rpt
GLNYAVFGLGN NCPR_MUSDO 162 19 -
GVKYAVFGLGN NCPR_CAVPO 164 19 -
GVKYAVFGLGN NCPR_PIG 164 19 -
GVKFAVFGLGN NCPR_MOUSE 164 19 -
GVKFAVFGLGN Q60451 154 19 -
GVKFAVFGLGN NCPR_RAT 164 19 -
GVKFAVFGLGN NCPR_HUMAN 164 19 -
GVKYAVFGLGN NCPR_RABIT 166 19 -
GLNYAVFGLGN Q27597 169 19 -
GLNYAVFGLGN P91655 170 19 -
TLRFCVFGLGS NOS3_HUMAN 643 64 -
TLRFCVFGLGS P70313 643 64 -
TLRFCVFGLGS NOS3_BOVIN 645 64 -
TFRYAVFGLGS NOS2_MOUSE 612 19 -
TFRYAVFGLGS O70516 264 19 -
TFRYAVFGLGS O70515 612 19 -
TFRYAVFGLGS O60604 615 19 -
TFRYAVFGLGS NOS2_RAT 615 19 -
TFRYAVFGLGS O54705 617 19 -
KFRYAVFGLGS NOS2_HUMAN 618 19 -
KFRYAVFGLGS O60757 618 19 -
GVRFAVFGLGN Q09590 158 19 -
TFRYAVFGLGS O60591 615 19 -
NLKYGVFGLGN Q39036 196 22 -
NLQYGVFGLGN O48938 182 22 -
NLQFGVFGLGN O24425 186 22 -
NVRFSVFGLGS P70594 910 95 -
NVRFSVFGLGS O19132 882 61 -
NVRFSVFGLGS NOS1_RAT 876 61 -
NVRFSVFGLGS NOS1_HUMAN 881 61 -
NIRYAVFGLGS NOS2_CHICK 615 19 -
KLTYGVFGLGN NCPR_PHAAU 174 23 -
QLTYGVFGLGN O24424 171 22 -
NVRFAVFALGS Q26240 634 69 -
TLRFCVFGLGS Q28969 646 64 -
KLQYGVFGLGN O48937 165 22 -
TLRYTVFGLGN NCPR_CANMA 144 19 -
NLQYGVFGLGN NCPR_CATRO 195 22 -
QLTYGVFALGN Q43235 175 23 -
NVRFAVFALGS O61608 707 80 -
NVRFAVFALGS Q27571 810 78 -
NVCFAVFALGS O77104 661 82 -
TLKYTVFGLGN NCPR_CANTR 144 19 -
GIRYAIFGLGN NCPR_SCHPO 148 30 -
NVRFAVFGLGS O61309 551 64 -
QLAYGVFALGN Q39035 176 22 -
KLKYVAFGLGN Q00141 160 29 -
QLPFAVLGLGD CYSJ_THIRO 141 20 -
NLRYNMFGLGN NCPR_YEAST 143 20 -
DLRFSVLALGD O32214 149 20 -
NTAFAVFSLGD CYSJ_ECOLI 144 20 -
NTAFAVFSLGD CYSJ_SALTY 144 20 -
ELKYGVFGFGD O14167 808 19 -
SLNVAVFGLGD YJ9F_YEAST 763 20 -
HLRYGLLGLGD O60471 88 22 -
NLDYVLLGLGD Q17574 88 22 -
GKKVSVFGCGD FLAV_DESSA 85 19 -
DKKVGVFGCGD FLAV_DESGI 85 19 -
HIQTAMLGLGD Q12181 98 30 -
GKTVALFGLGD FLAV_AZOVI 88 25 -
KGRAACFGCGD FLAV_DESVM 85 19 -
GKKVAYFGAGD FLAV_SYNY3 81 17 -
GRKVACFGCGD FLAV_DESVH 85 19 -
GKIVALYGLGD FLAW_ECOLI 79 17 -

Motif 4 width=20
Element Seqn Id St Int Rpt
DKRLEELGATRVFELGLGDD NCPR_MUSDO 186 13 -
DQRLEQLGAQRIFELGLGDD NCPR_CAVPO 188 13 -
DKRLEQLGAQRIFDLGLGDD NCPR_PIG 188 13 -
DQRLEQLGAQRIFELGLGDD NCPR_MOUSE 188 13 -
DQRLEQLGAQRIFELGLGDD Q60451 178 13 -
DQRLEQLGAQRIFELGLGDD NCPR_RAT 188 13 -
DKRLEQLGAQRIFELGLGDD NCPR_HUMAN 188 13 -
DQRLEQLGAQRIFELGMGDD NCPR_RABIT 190 13 -
DKRLEELGANRVFELGLGDD Q27597 193 13 -
DKRLEELGANRVFELGLGDD P91655 194 13 -
DTRLEELGGERLLQLGQGDE NOS3_HUMAN 667 13 -
DTRLEELGGERLLQLGQGDE P70313 667 13 -
DTRLEELGGERLLQLGQGDE NOS3_BOVIN 669 13 -
DQKLSHLGASQLAPTGEGDE NOS2_MOUSE 636 13 -
DQKLSHLGASQLAPTGEGDE O70516 288 13 -
DQKLSHLGASQLAPTGEGDE O70515 636 13 -
DQKLSHLGASQLAPTGEGDE O60604 639 13 -
DQKLSHLGASQLAPTGEGDE NOS2_RAT 639 13 -
DIKLSQLGASQLTPVGEGDE O54705 641 13 -
DQKLSHLGASQLTPMGEGDE NOS2_HUMAN 642 13 -
DQKLSHLGASQLTPMGEGDE O60757 642 13 -
DKQLEKLGAKRIFHLGLGDD Q09590 182 13 -
DPKLSHLGASQLAPTGEGDE O60591 639 13 -
DDILVEQGAQRLVQVGLGDD Q39036 220 13 -
DDGLVEQGAKRLVPVGMGDD O48938 206 13 -
DEILTEQGGKRIVPVGLGDD O24425 210 13 -
DTLLEELGGERILKMREGDE P70594 934 13 -
DTLLEELGGERILKMREGDE O19132 906 13 -
DTLLEELGGERILKMREGDE NOS1_RAT 900 13 -
DTLLEELGGERILKMREGDE NOS1_HUMAN 905 13 -
DQKLSQLGALQLTPVGEGDE NOS2_CHICK 639 13 -
DEELAEQGAKRLVAVGLGDD NCPR_PHAAU 198 13 -
DEQLGKQGAKRIVQVGLGDD O24424 195 13 -
DNLLGELGGERLVKLTTGDE Q26240 658 13 -
DTRLEELGGERLLQLGQGDE Q28969 670 13 -
DDGLADQGAKRIVEVGMGDD O48937 189 13 -
DRLLEEKGGERFADYGEGDD NCPR_CANMA 168 13 -
DEKVAEQGGKRIVPLVLGDD NCPR_CATRO 219 13 -
DEDLTEQGAKRLVPVGLGDD Q43235 199 13 -
DNILGELGGERLMKMATGDE O61608 731 13 -
DNILGELGGERLLRVAYGDE Q27571 834 13 -
DKVLGDLGGERILELACGDE O77104 685 13 -
DRLLGEKGGDRFAEYGEGDD NCPR_CANTR 168 13 -
DAAMTRLGATRVGNLGLGDD NCPR_SCHPO 172 13 -
YLMLQELGAERLVNYCAGDA O61309 575 13 -
DEELCKKGAKRLIEVGLGDD Q39035 200 13 -
DAAFQKLGPQRIGSAGEGDD Q00141 184 13 -
DRRLAELGAQRILPLQCCAL CYSJ_THIRO 165 13 -
EKHLSAAGAIRLGKLGEADD NCPR_YEAST 167 13 -
DQRLEELGGKRISPRVDCDL O32214 173 13 -
DSKLAELGGERLLDRVDADV CYSJ_ECOLI 168 13 -
DSKLAELGGERLLDRVDADV CYSJ_SALTY 168 13 -
DARFVELGAAPLVTLGLGND O14167 839 20 -
FKRLELLSAKALIPLGLGDD YJ9F_YEAST 794 20 -
DKRLQELGARHFYDTGHADD O60471 112 13 -
DKQLTALGANRLFDRAEADD Q17574 112 13 -
EEKLEKMGAVVIGDSLKIDG FLAV_DESSA 109 13 -
TYFCGAVDVIEKKAEELGAT FLAV_DESGI 99 3 -
QRIVTQLGANELFDRLEADD Q12181 123 14 -
ENYLDALGELYSFFKDRGAK FLAV_AZOVI 104 5 -
EERLSGLGADIVADSLKIDG FLAV_DESVM 109 13 -
DNFQDAMGILEEKISGLGGK FLAV_SYNY3 97 5 -
EEKLKNLGAEIVQDGLRIDG FLAV_DESVH 109 13 -
EWFLDALGMLHDKLSTKGVK FLAW_ECOLI 95 5 -