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PR00111

Identifier
ABHYDROLASE  [View Relations]  [View Alignment]  
Accession
PR00111
No. of Motifs
4
Creation Date
28-JUL-1992  (UPDATE 13-JUN-1999)
Title
Alpha/beta hydrolase fold signature
Database References

PFAM; PF00561 abhydrolase
INTERPRO; IPR000073
UMBBD; e0033; e0139; e0397; e0223
PDB; 2DHC
SCOP; 2HDC
Literature References
1. OLLIS, D.L., CHEAH, E., CYGLER, M., DIJKSTRA, B., FROLOW, F., FRANKEN,
S.M., HAREL, M., REMINGTON, S.J., SILMAN, I., SCHRAG, J., SUSSMAN, J.L.,
VERSCHUEREN, K.H.G. AND GOLDMAN, A.
The alpha/beta hydrolase fold.
PROTEIN ENG. 5(3) 197-211 (1992).

Documentation
The alpha/beta hydrolase fold is common to a number of hydrolytic enzymes
of widely differing phylogenetic origin and catalytic function. The core
of each enzyme is an alpha/beta-sheet (rather than a barrel), containing 8
strands connected by helices [1]. The enzymes are believed to have diverged
from a common ancestor, preserving the arrangement of the catalytic
residues. All have a catalytic triad, the elements of which are borne on
loops, which are the best conserved structural features of the fold. 
 
ABHYDROLASE is a 4-element fingerprint that provides a signature for 
members of this diverse family of enzymes. The fingerprint was derived 
from an initial alignment of 3 sequences: motifs 2 and 4 include elements
of the catalytic triad. Three iterations on OWL17.0 were required to reach
convergence, at which point a true set comprising 10 sequences was
identified: this included hydrolases, dehalogenases, lipases, and a
depolymerase. Several partial matches were also found, including magnesium
chelatase, chloroperoxidase, atropine acylhydrolase, haloacetate
dehalogenase, and so on, all of which are likely to be family members. The
low level of similarity between the different sequence types means that the
fingerprint has not performed well, and has missed sequences that are
probably family members (e.g. epoxide hydrolases). It does, however,
provide a rough diagnostic tool.
 
An update on SPTR37_9f identified a true set of 29 sequences, and 44
partial matches.
Summary Information
  29 codes involving  4 elements
12 codes involving 3 elements
32 codes involving 2 elements
Composite Feature Index
429292929
3111078
216121422
1234
True Positives
ACOC_ALCEU    BPHD_BURCE    BPHD_PSES1    DMPD_PSESP    
LIP1_PSYIM LIP3_MORSP MHPC_ECOLI O05149
O05327 O06648 O33766 O51858
O69263 O69794 O82828 O82829
O85983 O86901 P96965 PHAB_PSEOL
Q43936 Q51514 Q52011 Q52036
Q52532 Q59324 Q59695 TODF_PSEPU
XYLF_PSEPU
True Positive Partials
Codes involving 3 elements
O07214 O24830 O82821 O85289
O86011 O87637 P73490 P91141
P96851 PRXC_STRLI Q52897 Q59093
Codes involving 2 elements
BCHO_RHOCA ESTE_PSEFL HALO_XANAU O05119
O05145 O05235 O06734 O23227
O28735 O29396 O31386 O33550
O53321 O54172 O66382 O67982
O69356 O73957 O86608 P74484
P77026 PRXC_PSEFL PRXC_PSEPY PRXC_SYNY3
PRXH_BPMD2 Q51980 Q53540 Q57172
THCF_RHOER YE63_SCHPO YKD7_CAEEL YU14_MYCTU
Sequence Titles
ACOC_ALCEU  PROBABLE DIHYDROLIPOAMIDE ACETYLTRANSFERASE (EC 2.3.1.12) (FAST- MIGRATING PROTEIN) (FMP) - ALCALIGENES EUTROPHUS. 
BPHD_BURCE 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) - BURKHOLDERIA CEPACIA (PSEUDOMONAS CEPACIA).
BPHD_PSES1 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) - PSEUDOMONAS SP. (STRAIN KKS102).
DMPD_PSESP 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE (EC 3.1.1.-) (HMSH) - PSEUDOMONAS SP. (STRAIN CF600).
LIP1_PSYIM LIPASE 1 PRECURSOR (EC 3.1.1.3) (TRIACYLGLYCEROL LIPASE) - PSYCHROBACTER IMMOBILIS.
LIP3_MORSP LIPASE 3 PRECURSOR (EC 3.1.1.3) (TRIACYLGLYCEROL LIPASE) - MORAXELLA SP.
MHPC_ECOLI 2-HYDROXY-6-KETONONA-2,4-DIENEDIOIC ACID HYDROLASE (EC 3.7.1.-) - ESCHERICHIA COLI.
O05149 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) (HOPD) - RHODOCOCCUS SP.
O05327 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE - PSEUDOMONAS SP.
O06648 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) - PSEUDOMONAS AZELAICA.
O33766 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE - SPHINGOMONAS SP.
O51858 2-HYDROXY-6-OXO-7-METHYLOCTA-2,4-DIENOATE HYDROLASE - PSEUDOMONAS PUTIDA.
O69263 2-HYDROXY-6-OXO-2,4-HEPTADIENOATE HYDROLASE - BURKHOLDERIA SP.
O69794 2-HYDROXY-6-OXOHEPTA-2,4-DIENOATE HYDROLASE - RALSTONIA SP.
O82828 ETBD1 - RHODOCOCCUS SP.
O82829 ETBD2 - RHODOCOCCUS SP.
O85983 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE - SPHINGOMONAS AROMATICIVORANS.
O86901 HYDROLASE, DXNB PROTEIN - SPHINGOMONAS SP.
P96965 2-HYDROXY-6-OXO-7-METHYLOCTA-2,4-DIENOATE HYDROLASE - PSEUDOMONAS FLUORESCENS.
PHAB_PSEOL POLY(3-HYDROXYALKANOATE) DEPOLYMERASE (EC 3.1.1.-) (PHA DEPOLYMERASE) (PHB DEPOLYMERASE) - PSEUDOMONAS OLEOVORANS.
Q43936 3-OXOADIPATE ENOL-LACTONASE II (EC 3.1.1.24) (ENOL-LACTONE HYDROLASE II) (BETA-KETOADIPATE ENOL-LACTONE HYDROLASE II) - ACINETOBACTER CALCOACETICUS.
Q51514 PHA-DEPOLYMERASE - PSEUDOMONAS AERUGINOSA.
Q52011 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID HYDROLASE - PSEUDOMONAS PSEUDOALCALIGENES.
Q52036 2-HYDROXY-6-PHENYLHEXA-2 - PSEUDOMONAS PUTIDA.
Q52532 PCBD - PSEUDOMONAS SP.
Q59324 BETA-D-GALACTOSIDASE (EC 3.2.1.23) (BETA-GALACTOSIDASE) (LACTASE) - COMAMONAS TESTOSTERONI (PSEUDOMONAS TESTOSTERONI).
Q59695 DIHYDROLIPOAMIDE ACETYLTRANSFERASE (EC 2.3.1.12) (DIHYDROLIPOAMIDE S-ACETYLTRANSFERASE) (LIPOATE ACETYLTRANSFERASE) (THIOLTRANSACETYLASE A) - PSEUDOMONAS PUTIDA.
TODF_PSEPU 2-HYDROXY-6-OXO-2,4-HEPTADIENOATE HYDROLASE (HOHH) (EC 3.1.1.-) - PSEUDOMONAS PUTIDA.
XYLF_PSEPU 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE (EC 3.1.1.-) (HMSH) - PSEUDOMONAS PUTIDA.

O07214 HYPOTHETICAL 36.9 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O24830 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE - ACINETOBACTER SP.
O82821 2-HYDROXY-6-OXO-6-(2'-AMINOPHENYL)HEXA-2, 4-DIENOATE HYDROLASE - PSEUDOMONAS STUTZERI (PSEUDOMONAS PERFECTOMARINA).
O85289 HYDROLASE - SPHINGOMONAS SP. CB3.
O86011 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE - SPHINGOMONAS AROMATICIVORANS.
O87637 LACTONE-SPECIFIC ESTERASE - PSEUDOMONAS FLUORESCENS.
P73490 2-HYDROXY-6-OXOHEPTA-2,4-DIENOATE HYDROLASE - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P91141 SIMILAR TO ACETYLTRANSFERASES - CAENORHABDITIS ELEGANS.
P96851 HYPOTHETICAL 31.9 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
PRXC_STRLI NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) (CPO-L) (CHLOROPEROXIDASE L) - STREPTOMYCES LIVIDANS.
Q52897 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 3.7.1.-) (HOPD HYDROLASE) - RHODOCOCCUS SP.
Q59093 3-OXOADIPATE ENOL-LACTONASE I (EC 3.1.1.24) (ENOL-LACTONE HYDROLASE I) (BETA-KETOADIPATE ENOL-LACTONE HYDROLASE I) - ACINETOBACTER CALCOACETICUS.

BCHO_RHOCA MAGNESIUM-CHELATASE 30 KD SUBUNIT - RHODOBACTER CAPSULATUS (RHODOPSEUDOMONAS CAPSULATA).
ESTE_PSEFL ARYLESTERASE (EC 3.1.1.2) (ARYL-ESTER HYDROLASE) - PSEUDOMONAS FLUORESCENS.
HALO_XANAU HALOALKANE DEHALOGENASE (EC 3.8.1.5) - XANTHOBACTER AUTOTROPHICUS.
O05119 PUTATIVE GLYCERATE KINASE AND PYRUVATE KINASE (PYKA) GENES, COMPLETE CDS (PYKA) - METHYLOBACTERIUM EXTORQUENS.
O05145 2-HYDROXY-6-KETONONA-2,4-DIENOATE HYDROLASE - RHODOCOCCUS GLOBERULUS.
O05235 HYPOTHETICAL 31.3 KD PROTEIN - BACILLUS SUBTILIS.
O06734 PUTATIVE - PROBABLE ESTERASE - BACILLUS SUBTILIS.
O23227 HYPOTHETICAL 41.9 KD PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O28735 CARBOXYLESTERASE (EST-2) - ARCHAEOGLOBUS FULGIDUS.
O29396 CARBOXYLESTERASE (EST-1) - ARCHAEOGLOBUS FULGIDUS.
O31386 B-KETOADIPATE ENOL-LACTONE HYDROLASE (EC 3.1.1.24) (3-OXOADIPATE ENOL-LACTONASE) (CARBOXYMETHYLBUTENOLIDE LACTONASE) - BRADYRHIZOBIUM JAPONICUM.
O33550 BCHO PROTEIN - RHODOBACTER SPHAEROIDES (RHODOPSEUDOMONAS SPHAEROIDES).
O53321 HYPOTHETICAL 32.1 KD PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
O54172 HYDROLASE - STREPTOMYCES COELICOLOR.
O66382 ESTERASE2 - ACETOBACTER PASTEURIANUS.
O67982 3-OXOADIPATE ENOL-LACTONE HYDROLASE/4-CARBOXYMUCONOLACTONE DECARBOXYLASE (EC 3.1.1.24) (3-OXOADIPATE ENOL-LACTONASE) (CARBOXYMETHYLBUTENOLIDE LACTONASE) - RHODOCOCCUS OPACUS.
O69356 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE - RHODOCOCCUS ERYTHROPOLIS.
O73957 LIPOLYTIC ENZYME - SULFOLOBUS ACIDOCALDARIUS.
O86608 3-OXOADIPATE ENOL-LACTONE HYDROLASE/4-CARBOXYMUCONOLACTONE DECARBOXYLASE - STREPTOMYCES COELICOLOR.
P74484 HYPOTHETICAL 25.5 KD PROTEIN - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
P77026 MACROLIDE 2'-PHOSPHOTRANSFERASE II - ESCHERICHIA COLI.
PRXC_PSEFL NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) (CPO-F) (CHLOROPEROXIDASE F) - PSEUDOMONAS FLUORESCENS.
PRXC_PSEPY NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) (CPO-P) (CHLOROPEROXIDASE P) - PSEUDOMONAS PYRROCINIA.
PRXC_SYNY3 PUTATIVE NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
PRXH_BPMD2 PUTATIVE NON-HEME HALOPEROXIDASE (EC 1.11.1.-) - MYCOBACTERIOPHAGE D29.
Q51980 HOMODA HYDROLASE - PSEUDOMONAS PUTIDA.
Q53540 ESTERASE - PSEUDOMONAS PUTIDA.
Q57172 HOMOLOGY TO HYDROLASES - ACINETOBACTER CALCOACETICUS.
THCF_RHOER NON-HEME HALOPEROXIDASE (EC 1.11.1.-) - RHODOCOCCUS ERYTHROPOLIS.
YE63_SCHPO HYPOTHETICAL 48.7 KD PROTEIN C6G10.03C IN CHROMOSOME I - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
YKD7_CAEEL HYPOTHETICAL 88.4 KD PROTEIN B0464.7 IN CHROMOSOME III - CAENORHABDITIS ELEGANS.
YU14_MYCTU PUTATIVE HALOALKANE DEHALOGENASE (EC 3.8.1.5) - MYCOBACTERIUM TUBERCULOSIS.
Scan History
OWL17_1    3  200  NSINGLE    
OWL18_0 1 200 NSINGLE
OWL19_1 1 200 NSINGLE
OWL26_0 1 100 NSINGLE
SPTR37_9f 2 100 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
GYRVLLPDAPGFNKSD BPHD_PSES1 62 62 -
GARVIAPDFFGFGKSD HALO_XANAU 73 73 -
SRRVIAPDMLGFGYSE DMPD_PSEPU 57 57 -

Motif 2 width=14
Element Seqn Id St Int Rpt
LVGNSMGGAGALNF BPHD_PSES1 107 29 -
IVVQDWGGFLGLTL HALO_XANAU 119 30 -
LVGNSFGGGIALAL DMPD_PSEPU 102 29 -

Motif 3 width=14
Element Seqn Id St Int Rpt
AIRHPERVRRLVLM DMPD_PSEPU 116 0 -
ALEYPERTGKLILM BPHD_PSES1 121 0 -
PMADPSRFKRLIIM HALO_XANAU 133 0 -

Motif 4 width=15
Element Seqn Id St Int Rpt
TLVTWGRDDRFVPLD BPHD_PSES1 228 93 -
TLVIHGREDQIIPLQ DMPD_PSEPU 219 89 -
TFMAIGMKDKLLGPD HALO_XANAU 251 104 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
SRRVIAPDMLGFGYSE DMPD_PSESP 58 58 -
RRRVIAPDMVGFGFTQ O69794 56 56 -
TRRVIAPDMLGFGYSE XYLF_PSEPU 57 57 -
HRRVIAPDMVGFGFTQ TODF_PSEPU 56 56 -
RRRVIAPDMVGFGFTE O69263 56 56 -
GYRVILKDSPGFNKSD Q59324 63 63 -
GYRVILKDSPGFNKSD Q52011 63 63 -
RFRVLAPDMVGFGGTE O82828 56 56 -
RFRVLAPDMVGFGGTE O82829 56 56 -
GYRVLLPDAPGFNKSD BPHD_PSES1 62 62 -
GYRVILKDSPGFNKSD Q52036 63 63 -
GFRVILLDCPGFNKTD O06648 63 63 -
GYRVILKDSPGFNKSD BPHD_BURCE 63 63 -
DFRVIAPDMFGFGYSD O85983 61 61 -
DFRVIAPDMFGFGYSD O05327 64 64 -
DFRVIAPDMFGFGYSD O33766 61 61 -
FYRVIAPDMVGFGFTD P96965 54 54 -
NFRVIAADIVGFGFTD O51858 54 54 -
NFFVVAPDLIGFGQSE O05149 58 58 -
DLEVIAFDVPGVGGSS PHAB_PSEOL 54 54 -
ELEVIAFDVPGVGGSS Q51514 54 54 -
ERRVIALDLPGHGESA Q59695 159 159 -
GYHLIIPDLLGFGESS LIP1_PSYIM 92 92 -
GYHLIIPDLLGFGNSS LIP3_MORSP 92 92 -
DRRVIALDMVGFGYSD O86901 54 54 -
GYRVILLDCPGWGKSD MHPC_ECOLI 86 86 -
AYTVVALDLPGHGQSS ACOC_ALCEU 160 160 -
GYRVLLIDSPGFNKSY Q52532 63 63 -
KYFVICYDTRGHGASS Q43936 53 53 -

Motif 2 width=14
Element Seqn Id St Int Rpt
LVGNSFGGGIALAL DMPD_PSESP 103 29 -
LVGNSFGGALSLAF O69794 101 29 -
IVGNSFGGGLALAL XYLF_PSEPU 102 29 -
LVGNSFGGALSLAF TODF_PSEPU 101 29 -
FVGNSFGGGLSLAF O69263 101 29 -
LVGNSMGGATALNF Q59324 108 29 -
LVGNSMGGATALNF Q52011 108 29 -
LVGNSFGGAIALRV O82828 101 29 -
LVGNSFGGAIALRV O82829 101 29 -
LVGNSMGGAGALNF BPHD_PSES1 107 29 -
LVGNSMGGATALNF Q52036 108 29 -
LVGNSMGGATALNF O06648 108 29 -
LVGNSMGGATALNF BPHD_BURCE 108 29 -
IVGNSFGGGITLAF O85983 105 28 -
MVGNSFGGGITLAF O05327 108 28 -
MVGNSFGGGITLAF O33766 105 28 -
IVGNSFGGGLAIAT P96965 99 29 -
IVGNSFGGALAIAI O51858 99 29 -
IVGNSMGGAVTLQL O05149 107 33 -
VIGVSWGGALAQQF PHAB_PSEOL 98 28 -
AIGVSWGGALAQQF Q51514 98 28 -
LAGHSMGGAVSLNV Q59695 203 28 -
VGGNSMGGAISVAY LIP1_PSYIM 138 30 -
VGGNSMGGAISVAY LIP3_MORSP 138 30 -
IIGNSMGGRTTLAT O86901 105 35 -
LLGNSMGGHSSVAF MHPC_ECOLI 131 29 -
VVGHSMGGGVAAQL ACOC_ALCEU 204 28 -
MIGNSMGGATAMRM Q52532 107 28 -
FCGISMGGLTGQWL Q43936 97 28 -

Motif 3 width=14
Element Seqn Id St Int Rpt
AIRHPERVRRLVLM DMPD_PSESP 117 0 -
AIRFPHRVRRLVLM O69794 115 0 -
AIRHPERVRRLVLM XYLF_PSEPU 116 0 -
AIRFPHRVRRLVLM TODF_PSEPU 115 0 -
AIRFPHRVRRLVLM O69263 115 0 -
AIEYPERLGKMILM Q59324 122 0 -
AIEYPERIGKLILM Q52011 122 0 -
ATQHPERVGRLALM O82828 115 0 -
ATQHPERVERLALM O82829 115 0 -
ALEYPERTGKLILM BPHD_PSES1 121 0 -
AIEYPDRIGKLILM Q52036 122 0 -
ALEFPDRLDRLVLM O06648 122 0 -
ALEYPDRIGKLILM BPHD_BURCE 122 0 -
MIAHPDRVERAVLM O85983 119 0 -
MIAHPDRVERAVLM O05327 122 0 -
MIAHPDRVERAVLM O33766 119 0 -
ALRYSERVDRMVLM P96965 113 0 -
AIRHPGRIDRMVLM O51858 113 0 -
VVEAPERFDKVALM O05149 121 0 -
AHDYPERCKKLVLA PHAB_PSEOL 112 0 -
AHDYPERCKKLILA Q51514 112 0 -
AGLAPQRVASLSLI Q59695 217 0 -
AAKYPKDVKSLWLV LIP1_PSYIM 152 0 -
AAKYPKEIKSLWLV LIP3_MORSP 152 0 -
AIRRPDLVGDMVLM O86901 119 0 -
TLKWPERVGKLVLM MHPC_ECOLI 145 0 -
AVDAPQRVLSVALV ACOC_ALCEU 218 0 -
AVDYPEMVGKLVMM Q52532 121 0 -
AIHFPERFNQVIVA Q43936 111 0 -

Motif 4 width=15
Element Seqn Id St Int Rpt
TLVIHGREDQIIPLQ DMPD_PSESP 220 89 -
TLIVHGRDDRVIPLE O69794 218 89 -
TLVIHGREDRIIPLQ XYLF_PSEPU 219 89 -
TLILHGRDDRVIPLE TODF_PSEPU 218 89 -
TLILHGRDDRVVPLE O69263 218 89 -
TLVTWGRDDRFVPLD Q59324 229 93 -
TFITWGRDDRFVPLD Q52011 229 93 -
TLVIHGREDRVVPLS O82828 218 89 -
TLVIHGREDRVVPLS O82829 218 89 -
TLVTWGRDDRFVPLD BPHD_PSES1 228 93 -
TFITWGRDDRFVPLD Q52036 229 93 -
TLVTWGRDDRFVPLD O06648 228 92 -
TFITWGRDDRFVPLD BPHD_BURCE 229 93 -
TLILHGIADQVIPLD O85983 223 90 -
TLILHGIADQVIPLD O05327 226 90 -
TLILHGIADQVIPLD O33766 223 90 -
TLIIHGREDQVVPLS P96965 216 89 -
TLIIHGREDQVVPLS O51858 216 89 -
VLVFHGRQDRIVPLD O05149 228 93 -
TLVLAGDDDPLIPLI PHAB_PSEOL 213 87 -
TLVLAGDDDPIIPLI Q51514 213 87 -
ALVVWGGKDAIIPAS Q59695 317 86 -
TLVVWGDKDQIIKPE LIP1_PSYIM 256 90 -
TLVVWGDKDQVIKPE LIP3_MORSP 256 90 -
TLVVNGKADKVVPPE O86901 220 87 -
TLIVWGRNDRFVPMD MHPC_ECOLI 252 93 -
VLVVWGGQDQIIPAA ACOC_ALCEU 320 88 -
TMIIWGRDDRFVPLD Q52532 227 92 -
VLVIAGAQDPVTTVA Q43936 210 85 -