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PR00084

Identifier
MTLDHDRGNASE  [View Relations]  [View Alignment]  
Accession
PR00084
No. of Motifs
4
Creation Date
01-MAR-1995  (UPDATE 28-JUL-1999)
Title
Mannitol dehydrogenase signature
Database References

PROSITE; PS00974 MANNITOL_DHGENASE
PFAM; PF01232 Mannitol_dh
INTERPRO; IPR000669
Literature References
1. FISCHER, R., VAN STRANDMANN, R.P. AND HENGSTENBERG, W.
Mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system
of Enterococcus faecalis: molecular cloning and nucleotide sequences of the
enzyme IIIMtl gene and the mannitol-1-phosphate dehydrogenase gene,
expression in Escherichia coli, and comparison of the gene products with 
similar enzymes.
J.BACTERIOL. 173 3709-3715 (1991).
 
2. HONEYMAN, A.L. AND CURTISS, R.
Isolation, characterization, and nucleotide sequence of the Streptococcus
mutans mannitol-phosphate dehydrogenase gene and the mannitol-specific
factor III gene of the phosphoenolpyruvate phosphotransferase system.
INFECT.IMMUN. 60 3369-3375 (1992).
 
3. SCNEIDER, K.H., GIFFHORN, F. AND KAPLAN, S.
Cloning, nucleotide sequence and characterization of the mannitol
dehydrogenase gene from Rhodobacter sphaeroides.
J.GEN.MICROBIOL. 139 2475-2484 (1993).

Documentation
Mannitol-1-phosphate 5-dehydrogenase catalyses the NAD-dependent reduction
of mannitol-1-phosphate to fructose-6-phosphate [1] as part of the
phosphoenolpyruvate-dependent phosphotransferase system (PTS) [2]. The PTS
facilitates the vectorial translocation of metabolisable carbohydrates to
form the corresponding sugar phosphates, which are then converted to 
glycolytic intermediates [2]. Mannitol 2-dehydrogenase catalyses the 
NAD-dependent reduction of mannitol to fructose [3]. The E.coli yeiQ
protein shares sequence similarity with these 2 types of dehydrogenase,
suggesting an evolutionary relationship.
 
MTLDHDRGNASE is a 4-element fingerprint that provides a signature for
mannitol dehydrogenases. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions in
the first half of the alignment, motif 1 including a putative NAD-binding
site, and motif 3 containing the region encoded by PROSITE pattern
MANNITOL_DHGENASE (PS00974). Two iterations on OWL25.1 were required to
reach convergence, at which point a true set comprising 7 sequences was
identified. A single partial match was also found, ECMTLD1, an E.coli
mannitol-1-phosphate dehydrogenase that fails to make significant matches
with motifs 2 and 4.
 
An update on SPTR37_9f identified a true set of 14 sequences, and 1
partial match.
Summary Information
  14 codes involving  4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
414141414
31011
20000
1234
True Positives
MTLD_BACST    MTLD_BACSU    MTLD_ECOLI    MTLD_ENTFA    
MTLD_STRMU MTLK_RHOSH O08355 O52720
O65992 O68111 UXUB_ECOLI YDFI_ECOLI
YEI0_YEAST YEIQ_ECOLI
True Positive Partials
Codes involving 3 elements
O34354
Sequence Titles
MTLD_BACST  MANNITOL-1-PHOSPHATE 5-DEHYDROGENASE (EC 1.1.1.17) - BACILLUS STEAROTHERMOPHILUS. 
MTLD_BACSU MANNITOL-1-PHOSPHATE 5-DEHYDROGENASE (EC 1.1.1.17) - BACILLUS SUBTILIS.
MTLD_ECOLI MANNITOL-1-PHOSPHATE 5-DEHYDROGENASE (EC 1.1.1.17) - ESCHERICHIA COLI.
MTLD_ENTFA MANNITOL-1-PHOSPHATE 5-DEHYDROGENASE (EC 1.1.1.17) - ENTEROCOCCUS FAECALIS (STREPTOCOCCUS FAECALIS).
MTLD_STRMU MANNITOL-1-PHOSPHATE 5-DEHYDROGENASE (EC 1.1.1.17) - STREPTOCOCCUS MUTANS.
MTLK_RHOSH MANNITOL 2-DEHYDROGENASE (EC 1.1.1.67) (MDH) - RHODOBACTER SPHAEROIDES (RHODOPSEUDOMONAS SPHAEROIDES).
O08355 MANNITOL 2-DEHYDROGENASE (EC 1.1.1.67) (MDH) - PSEUDOMONAS FLUORESCENS.
O52720 D-ARABINITOL DEHYDROGENASE - KLEBSIELLA PNEUMONIAE.
O65992 MTLD (EC 1.1.1.17) - CLOSTRIDIUM ACETOBUTYLICUM.
O68111 MANNITOL 2-DEHYDROGENASE (EC 1.1.1.67) - RHODOBACTER CAPSULATUS (RHODOPSEUDOMONAS CAPSULATA).
UXUB_ECOLI D-MANNONATE OXIDOREDUCTASE (EC 1.1.1.57) (FRUCTURONATE REDUCTASE) - ESCHERICHIA COLI.
YDFI_ECOLI HYPOTHETICAL 53.7 KD PROTEIN IN DCP-NOHA INTERGENIC REGION - ESCHERICHIA COLI.
YEI0_YEAST HYPOTHETICAL 56.5 KD PROTEIN IN HXT8 5'REGION AND IN PAU6 5'REGION - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
YEIQ_ECOLI HYPOTHETICAL 54.0 KD PROTEIN IN FRUB-RTN INTERGENIC REGION - ESCHERICHIA COLI.

O34354 HYPOTHETICAL ALTRONATE OXIDOREDUCTASE - BACILLUS SUBTILIS.
Scan History
OWL25_1    2  100  NSINGLE    
SPTR37_9f 3 50 NSINGLE
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
IVHIGVGNFHR MTLK_RHOSH 18 18 -
AVHFGAGNIGR MTLD_STRMU 4 4 -
AVHFGAGNIGR MTLD_ENTFA 3 3 -
ALHFGAGNIGR MTLD_ECOLI 3 3 -

Motif 2 width=14
Element Seqn Id St Int Rpt
PFTVMSCDNLPGNG MTLK_RHOSH 170 141 -
PLDIIACENMIGGS MTLD_STRMU 117 102 -
PLDIIACENMIGGS MTLD_ENTFA 117 103 -
PLNIIACENMVRGT MTLD_ECOLI 114 100 -

Motif 3 width=14
Element Seqn Id St Int Rpt
VAFPNGMVDRITPA MTLK_RHOSH 209 25 -
IGFPNAAVDRIVPA MTLD_STRMU 153 22 -
IGFPDAAVDRIVPL MTLD_ENTFA 151 20 -
VGFVDSAVDRIVPP MTLD_ECOLI 150 22 -

Motif 4 width=16
Element Seqn Id St Int Rpt
DPVPVTCEPFRQWVIE MTLK_RHOSH 238 15 -
DPLFVVVEPFSEWVVE MTLD_STRMU 171 4 -
DPLFVQVEPFCEWVID MTLD_ENTFA 169 4 -
DPLEVTVETFSEWIVD MTLD_ECOLI 170 6 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
IVHIGVGNFHR MTLK_RHOSH 18 18 -
IVHLGFGAFHR YDFI_ECOLI 24 24 -
AVHFGAGNIGR MTLD_STRMU 4 4 -
IVHIGLGNFHR O68111 19 19 -
IVHFGFGAFHR YEIQ_ECOLI 27 27 -
AVHFGAGNIGR MTLD_BACST 3 3 -
IVHLGCGAFHR UXUB_ECOLI 25 25 -
AVHFGAGNIGR MTLD_ENTFA 3 3 -
IVHLGVGAFHR YEI0_YEAST 38 38 -
ALHFGAGNIGR MTLD_BACSU 3 3 -
IAHIGVGGFHR O08355 29 29 -
ALHFGAGNIGR MTLD_ECOLI 3 3 -
ALHFGAGNIGR O65992 3 3 -
WLHIGLGSFHR O52720 7 7 -

Motif 2 width=14
Element Seqn Id St Int Rpt
PFTVMSCDNLPGNG MTLK_RHOSH 170 141 -
AFTVMSCDNMPENG YDFI_ECOLI 176 141 -
PLDIIACENMIGGS MTLD_STRMU 117 102 -
PFTVMCCDNVPHNG O68111 172 142 -
PFTVLSCDNIPDNG YEIQ_ECOLI 179 141 -
PLHIIACENMIGGS MTLD_BACST 115 101 -
AFTVMSCDNVRENG UXUB_ECOLI 179 143 -
PLDIIACENMIGGS MTLD_ENTFA 117 103 -
PFTIMSCDNMPQNG YEI0_YEAST 191 142 -
TLNIIACENMIGGS MTLD_BACSU 113 99 -
AFTVMSCDNLPHNG O08355 183 143 -
PLNIIACENMVRGT MTLD_ECOLI 114 100 -
PLDIIACENALFAS O65992 114 100 -
PLTLLNCDNVRHNG O52720 157 139 -

Motif 3 width=14
Element Seqn Id St Int Rpt
VAFPNGMVDRITPA MTLK_RHOSH 209 25 -
VTFPSTMVDRIVPA YDFI_ECOLI 215 25 -
IGFPNAAVDRIVPA MTLD_STRMU 153 22 -
VAFPNSMVDRITPA O68111 211 25 -
VSFPGTMVDRIVPA YEIQ_ECOLI 218 25 -
YGFLNCAVDRIVPN MTLD_BACST 151 22 -
VTFPCTMVDRIVPA UXUB_ECOLI 218 25 -
IGFPDAAVDRIVPL MTLD_ENTFA 151 20 -
VTSPNSMVDRVTPR YEI0_YEAST 231 26 -
LGFPNSAVDRIVPI MTLD_BACSU 149 22 -
VSFPNAMVDRITPM O08355 222 25 -
VGFVDSAVDRIVPP MTLD_ECOLI 150 22 -
VGFPNCTVDRIVPN O65992 150 22 -
TTCPNTMVDRITPR O52720 197 26 -

Motif 4 width=16
Element Seqn Id St Int Rpt
DPVPVTCEPFRQWVIE MTLK_RHOSH 238 15 -
DPAGVACEPFRQWVIE YDFI_ECOLI 245 16 -
DPLFVVVEPFSEWVVE MTLD_STRMU 171 4 -
DAAPVTCETFRQWVIE O68111 240 15 -
DPCAISCEPFIQWVVE YEIQ_ECOLI 248 16 -
DPLSVVVEPFFEWVIE MTLD_BACST 169 4 -
DPCAIACEPFRQWVIE UXUB_ECOLI 248 16 -
DPLFVQVEPFCEWVID MTLD_ENTFA 169 4 -
DQCPVVAEPFIQWVLE YEI0_YEAST 261 16 -
DPLKVSVEPFFEWVID MTLD_BACSU 167 4 -
DAWPVVCEPFVQWVLE O08355 252 16 -
DPLEVTVETFSEWIVD MTLD_ECOLI 170 6 -
LPIDVAVEDFYEWDIE O65992 170 6 -
DKAPVMGETFIQWVVE O52720 227 16 -