WORKLIST ENTRIES (1):
WNT8PROTEIN View alignment Wnt-8 protein signature
Type of fingerprint: COMPOUND with 4 elements
Links:
PRINTS; PR01349 WNTPROTEIN
PRINTS; PR01841 WNT1PROTEIN; PR01842 WNT2PROTEIN; PR01843 WNT3PROTEIN
PRINTS; PR01844 WNT4PROTEIN; PR01845 WNT6PROTEIN; PR01891 WNT7PROTEIN
PRINTS; PR01893 WNT10PROTEIN; PR01894 WNT14PROTEIN; PR01895 WNT16PROTEIN
MIM; 606360; 601396
Creation date 13-MAY-2003
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.REV. 33 1-12 (2000).
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
7. CHRISTIAN, J.L., MCMAHON, J.A., MCMAHON, A.P. AND MOON, R.T.
Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing
growth factors, may play a role in ventral mesodermal patterning during
embryogenesis.
DEVELOPMENT 111 1045-1055 (1991).
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidney,
regulation of mammary gland proliferation, and Alzheimer's disease [3,4].
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been
identified in vertebrates, with multiple subtypes within some classes.
Wnt-8 cDNA was first isolated from Xenopus, with orthologues later being
identified in several other species including human, mouse and zebrafish.
Alternative exon splicing gives rise to several Wnt-8 isoforms [7].
WNT8PROTEIN is a 4-element fingerprint that provides a signature for Wnt-8
proteins. The fingerprint was derived from an initial alignment of 10
sequences: the motifs were drawn from conserved regions spanning the full
alignment length, focusing on those sections that characterise Wnt-8
proteins but distinguish them from other Wnt subtypes - motif 1 lies at the
N-terminus, following the putative signal peptide; motifs 2 and 3 reside
between conserved cysteines; and motif 4 includes conserved cysteines
13-15. Two iterations on SPTR40_22f were required to reach convergence,
at which point a true set comprising 12 sequences was identified.
SUMMARY INFORMATION
12 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 12 12 12 12
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
Q90ZA3 WNT8_BRARE WN8B_XENLA WN8C_CHICK
WN8B_MOUSE WNT8_XENLA WN8B_HUMAN Q90YJ8
Q90YL8 WN8B_BRARE WN8A_HUMAN WN8A_MOUSE
PROTEIN TITLES
Q90ZA3 Secreted factor Axwnt-8 - Ambystoma mexicanum (Axolotl).
WNT8_BRARE Wnt-8 protein precursor - Brachydanio rerio (Zebrafish) (Dan
WN8B_XENLA WNT-8B protein precursor (XWNT-8B) - Xenopus laevis (African
WN8C_CHICK WNT-8C protein precursor (CWNT-8) - Gallus gallus (Chicken).
WN8B_MOUSE WNT-8B protein precursor - Mus musculus (Mouse).
WNT8_XENLA WNT-8 protein precursor (XWNT-8) - Xenopus laevis (African c
WN8B_HUMAN WNT-8B protein precursor - Homo sapiens (Human).
Q90YJ8 Wnt8-like protein 2 - Brachydanio rerio (Zebrafish) (Danio r
Q90YL8 Wnt8-like protein 2 - Brachydanio rerio (Zebrafish) (Danio r
WN8B_BRARE Wnt-8b protein precursor - Brachydanio rerio (Zebrafish) (Da
WN8A_HUMAN WNT-8A protein precursor (WNT-8D) - Homo sapiens (Human).
WN8A_MOUSE WNT-8A protein precursor (WNT-8D) (Stimulated by retinoic ac
SCAN HISTORY
SPTR40_22f 2 100 NSINGLE
INITIAL MOTIF SETS
WNT8PROTEIN1 Length of motif = 13 Motif number = 1
Wnt-8 protein motif I - 1
PCODE ST INT
LMTGPKAYLIYSS WN8B_BRARE 30 30
LMTGPKAYLIYSS WN8B_XENLA 29 29
LITGPKAYLTYTT WN8A_HUMAN 29 29
LMTGPKAYLTYSS WN8C_CHICK 30 30
LMTGPKAYLIYSS WN8B_HUMAN 29 29
LITGPKAYLTYTA WN8A_MOUSE 29 29
LMTGPKAYLAYTS WNT8_BRARE 30 30
LMTGPKAYLVYSS WN8B_MOUSE 28 28
LMTGPKAYLTYSA WNT8_XENLA 30 30
LITGPKAYLTYTT WN8A_HUMAN 29 29
WNT8PROTEIN2 Length of motif = 10 Motif number = 2
Wnt-8 protein motif II - 1
PCODE ST INT
QRGGQGWLWG WN8B_BRARE 122 79
QLGGQGWLWG WN8B_XENLA 194 152
KTGGHGWIWG WN8A_HUMAN 121 79
RVGGRGWVWG WN8C_CHICK 122 79
QLGGQGWLWG WN8B_HUMAN 121 79
KTGGHGWIWG WN8A_MOUSE 121 79
KMGGRGWVWG WNT8_BRARE 122 79
QLGGQGWLWG WN8B_MOUSE 120 79
RIGGRGWVWG WNT8_XENLA 122 79
KTGGHGWIWG WN8A_HUMAN 121 79
WNT8PROTEIN3 Length of motif = 15 Motif number = 3
Wnt-8 protein motif III - 1
PCODE ST INT
AGNSAASRGAIAETF WN8B_BRARE 225 93
AGNSAASRGAIAETF WN8B_XENLA 297 93
AGNSAEGHWVPAEAF WN8A_HUMAN 227 96
AGNSADSRGATAETF WN8C_CHICK 228 96
AGNSAAARGAIADTF WN8B_HUMAN 224 93
AGNRAEGRWALTEAF WN8A_MOUSE 227 96
AGNSADNRGAIADTF WNT8_BRARE 228 96
AGNSAAGRGAIADTF WN8B_MOUSE 223 93
SGNSADNRGAIADAF WNT8_XENLA 228 96
AGNSAEGHWVPAEAF WN8A_HUMAN 227 96
WNT8PROTEIN4 Length of motif = 15 Motif number = 4
Wnt-8 protein motif IV - 1
PCODE ST INT
SKWEKRTCKRLCGDC WN8B_BRARE 281 41
SKWEKRSCRRLCGDC WN8B_XENLA 353 41
SRWERRSCGRLCTEC WN8A_HUMAN 283 41
SQWERRSCRRLSTEC WN8C_CHICK 284 41
GRWELRSCRRLCGDC WN8B_HUMAN 280 41
SRREQRSCGRLCTEC WN8A_MOUSE 283 41
SQWERRSCRRLCHEC WNT8_BRARE 284 41
GRWERRSCRRLCGDC WN8B_MOUSE 279 41
SQWERRSCKRLCTDC WNT8_XENLA 284 41
SRWERRSCGRLCTEC WN8A_HUMAN 283 41
FINAL MOTIF SETS
WNT8PROTEIN1 Length of motif = 13 Motif number = 1
Wnt-8 protein motif I - 2
PCODE ST INT
LMTGPKAYLTYST Q90ZA3 30 30
LMTGPKAYLAYTS WNT8_BRARE 30 30
LMTGPKAYLIYSS WN8B_XENLA 29 29
LMTGPKAYLTYSS WN8C_CHICK 30 30
LMTGPKAYLVYSS WN8B_MOUSE 28 28
LMTGPKAYLTYSA WNT8_XENLA 30 30
LMTGPKAYLIYSS WN8B_HUMAN 29 29
LITGPKAYLTYAN Q90YJ8 29 29
LITGPKAYLTYAN Q90YL8 29 29
LMTGPKAYLIYSS WN8B_BRARE 30 30
LITGPKAYLTYTT WN8A_HUMAN 29 29
LITGPKAYLTYTA WN8A_MOUSE 29 29
WNT8PROTEIN2 Length of motif = 10 Motif number = 2
Wnt-8 protein motif II - 2
PCODE ST INT
RMGGRGWVWG Q90ZA3 122 79
KMGGRGWVWG WNT8_BRARE 122 79
QLGGQGWLWG WN8B_XENLA 194 152
RVGGRGWVWG WN8C_CHICK 122 79
QLGGQGWLWG WN8B_MOUSE 120 79
RIGGRGWVWG WNT8_XENLA 122 79
QLGGQGWLWG WN8B_HUMAN 121 79
RLGGRGWLWG Q90YJ8 121 79
RLGGRGWLWG Q90YL8 121 79
QRGGQGWLWG WN8B_BRARE 122 79
KTGGHGWIWG WN8A_HUMAN 121 79
KTGGHGWIWG WN8A_MOUSE 121 79
WNT8PROTEIN3 Length of motif = 15 Motif number = 3
Wnt-8 protein motif III - 2
PCODE ST INT
AGNSADNRGAIAEAF Q90ZA3 228 96
AGNSADNRGAIADTF WNT8_BRARE 228 96
AGNSAASRGAIAETF WN8B_XENLA 297 93
AGNSADSRGATAETF WN8C_CHICK 228 96
AGNSAAGRGAIADTF WN8B_MOUSE 223 93
SGNSADNRGAIADAF WNT8_XENLA 228 96
AGNSAAARGAIADTF WN8B_HUMAN 224 93
AGNSADNRVTMTDAF Q90YJ8 227 96
AGNSADNRVTMTDAF Q90YL8 227 96
AGNSAASRGAIAETF WN8B_BRARE 225 93
AGNSAEGHWVPAEAF WN8A_HUMAN 227 96
AGNRAEGRWALTEAF WN8A_MOUSE 227 96
WNT8PROTEIN4 Length of motif = 15 Motif number = 4
Wnt-8 protein motif IV - 2
PCODE ST INT
SQWEKRSCRRLCTEC Q90ZA3 284 41
SQWERRSCRRLCHEC WNT8_BRARE 284 41
SKWEKRSCRRLCGDC WN8B_XENLA 353 41
SQWERRSCRRLSTEC WN8C_CHICK 284 41
GRWERRSCRRLCGDC WN8B_MOUSE 279 41
SQWERRSCKRLCTDC WNT8_XENLA 284 41
GRWELRSCRRLCGDC WN8B_HUMAN 280 41
SQWERRSCRRLCHEC Q90YJ8 283 41
SQWERRSCRRLCHEC Q90YL8 283 41
SKWEKRTCKRLCGDC WN8B_BRARE 281 41
SRWERRSCGRLCTEC WN8A_HUMAN 283 41
SRREQRSCGRLCTEC WN8A_MOUSE 283 41
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