WORKLIST ENTRIES (1):
WNT7PROTEIN View alignment Wnt-7 protein signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR01349 WNTPROTEIN
PRINTS; PR01841 WNT1PROTEIN; PR01842 WNT2PROTEIN; PR01843 WNT3PROTEIN
PRINTS; PR01844 WNT4PROTEIN; PR01845 WNT6PROTEIN; PR01892 WNT8PROTEIN
PRINTS; PR01893 WNT10PROTEIN; PR01894 WNT14PROTEIN; PR01895 WNT16PROTEIN
MIM; 601570; 601967
Creation date 12-MAY-2003
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.REV. 33 1-12 (2000).
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
6. MOON, R.T.
An introduction to non-canonical Wnt and frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
7. GAVIN, B.J., MCMAHON, J.A. AND MCMAHON, A.P.
Expression of multiple novel Wnt-1/int-1-related genes during fetal and
adult mouse development.
GENES DEV. 4 2319-2332 (1990).I
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidney,
regulation of mammary gland proliferation, and Alzheimer's disease [3,4].
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been
identified in vertebrates, with multiple subtypes within some classes.
Wnt-7 cDNA was isolated from mouse using a PCR-based strategy, where it was
found to be expressed in adult tissues, particularly in brain and lung [7].
Two subtypes are known to exist, designated Wnt-7A and B. Wnt-7A has been
implicated in development of the uterus, cerebellum and limbs; Wnt-7B is
believed to play a role in lung and placental development.
WNT7PROTEIN is a 5-element fingerprint that provides a signature for Wnt-7
proteins. The fingerprint was derived from an initial alignment of 7
sequences: the motifs were drawn from conserved regions spanning the full
alignment length, focusing on those sections that characterise Wnt-6
proteins but distinguish them from other Wnt subtypes - motif 1 lies near
the N-terminus; motifs 2-4 are situated in the central portion of the
alignment, between conserved cysteines; and motif 5 resides at the C-
terminus. Two iterations on SPTR40_22f were required to reach convergence,
at which point a true set comprising 9 sequences was identified.
SUMMARY INFORMATION
9 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 9 9 9 9 9
4| 0 0 0 0 0
3| 0 0 0 0 0
2| 0 0 0 0 0
--+--------------------------
| 1 2 3 4 5
True positives..
Q9DBY3 WN7A_MOUSE Q96H90 Q9DEB8
WN7A_HUMAN O42258 WN7B_MOUSE O13266
WN7B_HUMAN
PROTEIN TITLES
Q9DBY3 Wingless-related MMTV integration site 7A - Mus musculus (Mo
WN7A_MOUSE WNT-7A protein precursor - Mus musculus (Mouse).
Q96H90 Hypothetical protein - Homo sapiens (Human).
Q9DEB8 Wnt-7a - Gallus gallus (Chicken).
WN7A_HUMAN WNT-7A protein precursor - Homo sapiens (Human).
O42258 Wnt7B - Xenopus laevis (African clawed frog).
WN7B_MOUSE WNT-7B protein precursor - Mus musculus (Mouse).
O13266 Wnt-7a - Pleurodeles waltlii (Iberian ribbed newt).
WN7B_HUMAN WNT-7B protein precursor - Homo sapiens (Human).
SCAN HISTORY
SPTR40_22f 2 100 NSINGLE
INITIAL MOTIF SETS
WNT7PROTEIN1 Length of motif = 15 Motif number = 1
Wnt-7 protein motif I - 1
PCODE ST INT
GIIYMKLGALSSVVA O42258 17 17
GIVYIKIGGFSSVVA Q9DEB8 17 17
GVLYVKLGALSSVVA WN7B_MOUSE 17 17
GIVYLRIGGFSSVVA WN7A_MOUSE 17 17
GIVYIKIGGFSSVVA O13266 17 17
GVLYVKLGALSSVVA WN7B_HUMAN 17 17
GMVCLRIGGFSSVVA WN7A_HUMAN 17 17
WNT7PROTEIN2 Length of motif = 12 Motif number = 2
Wnt-7 protein motif II - 1
PCODE ST INT
DREKQGYYNQEE O42258 134 102
DKEKQGQYHKEE Q9DEB8 134 102
DREKQGYYNQAE WN7B_MOUSE 134 102
DKEKQGQYHWDE WN7A_MOUSE 134 102
DKGKQGQFHIEE O13266 133 101
DREKQGYYNQAE WN7B_HUMAN 134 102
DKEKQGQYHRDE WN7A_HUMAN 134 102
WNT7PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-7 protein motif III - 1
PCODE ST INT
KVLEERMKLE O42258 190 44
KILEENMKLE Q9DEB8 190 44
KVLEDRMKLE WN7B_MOUSE 190 44
KILEENMKLE WN7A_MOUSE 190 44
KILSENLRLE O13266 189 44
KVLEDRMQLE WN7B_HUMAN 190 44
KILEENMKLE WN7A_HUMAN 190 44
WNT7PROTEIN4 Length of motif = 11 Motif number = 4
Wnt-7 protein motif IV - 1
PCODE ST INT
RSYQKPMETDL O42258 258 58
LSYRKPMDTDL Q9DEB8 258 58
RSYQKPMETDL WN7B_MOUSE 258 58
LSYRKPMDTDL WN7A_MOUSE 258 58
LSYLKPMDTDL O13266 257 58
RSYQKPMETDL WN7B_HUMAN 258 58
LSYRKPMDTDL WN7A_HUMAN 258 58
WNT7PROTEIN5 Length of motif = 10 Motif number = 5
Wnt-7 protein motif V - 1
PCODE ST INT
THQYTKVWQC O42258 315 46
THQYSRVWQC Q9DEB8 315 46
THQYTKVWQC WN7B_MOUSE 315 46
THQYARVWQC WN7A_MOUSE 315 46
THQYSRVWKC O13266 314 46
THQYTKVWQC WN7B_HUMAN 315 46
THQYARVWQC WN7A_HUMAN 315 46
FINAL MOTIF SETS
WNT7PROTEIN1 Length of motif = 15 Motif number = 1
Wnt-7 protein motif I - 2
PCODE ST INT
GIVYLRIGGFSSVVA Q9DBY3 17 17
GIVYLRIGGFSSVVA WN7A_MOUSE 17 17
GMVYLRIGGFSSVVA Q96H90 17 17
GIVYIKIGGFSSVVA Q9DEB8 17 17
GMVCLRIGGFSSVVA WN7A_HUMAN 17 17
GIIYMKLGALSSVVA O42258 17 17
GVLYVKLGALSSVVA WN7B_MOUSE 17 17
GIVYIKIGGFSSVVA O13266 17 17
GVLYVKLGALSSVVA WN7B_HUMAN 17 17
WNT7PROTEIN2 Length of motif = 12 Motif number = 2
Wnt-7 protein motif II - 2
PCODE ST INT
DKEKQGQYHRDE Q9DBY3 134 102
DKEKQGQYHWDE WN7A_MOUSE 134 102
DKEKQGQYHRDE Q96H90 134 102
DKEKQGQYHKEE Q9DEB8 134 102
DKEKQGQYHRDE WN7A_HUMAN 134 102
DREKQGYYNQEE O42258 134 102
DREKQGYYNQAE WN7B_MOUSE 134 102
DKGKQGQFHIEE O13266 133 101
DREKQGYYNQAE WN7B_HUMAN 134 102
WNT7PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-7 protein motif III - 2
PCODE ST INT
KILEENMKLE Q9DBY3 190 44
KILEENMKLE WN7A_MOUSE 190 44
KILEENMKLE Q96H90 190 44
KILEENMKLE Q9DEB8 190 44
KILEENMKLE WN7A_HUMAN 190 44
KVLEERMKLE O42258 190 44
KVLEDRMKLE WN7B_MOUSE 190 44
KILSENLRLE O13266 189 44
KVLEDRMQLE WN7B_HUMAN 190 44
WNT7PROTEIN4 Length of motif = 11 Motif number = 4
Wnt-7 protein motif IV - 2
PCODE ST INT
LSYRKPMDTDL Q9DBY3 258 58
LSYRKPMDTDL WN7A_MOUSE 258 58
LSYRKPMDTDL Q96H90 258 58
LSYRKPMDTDL Q9DEB8 258 58
LSYRKPMDTDL WN7A_HUMAN 258 58
RSYQKPMETDL O42258 258 58
RSYQKPMETDL WN7B_MOUSE 258 58
LSYLKPMDTDL O13266 257 58
RSYQKPMETDL WN7B_HUMAN 258 58
WNT7PROTEIN5 Length of motif = 10 Motif number = 5
Wnt-7 protein motif V - 2
PCODE ST INT
THQYARVWQC Q9DBY3 315 46
THQYARVWQC WN7A_MOUSE 315 46
THQYARVWQC Q96H90 315 46
THQYSRVWQC Q9DEB8 315 46
THQYARVWQC WN7A_HUMAN 315 46
THQYTKVWQC O42258 315 46
THQYTKVWQC WN7B_MOUSE 315 46
THQYSRVWKC O13266 314 46
THQYTKVWQC WN7B_HUMAN 315 46
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