WORKLIST ENTRIES (1):
WNT4PROTEIN View alignment Wnt-4 protein signature
Type of fingerprint: COMPOUND with 4 elements
Links:
PRINTS; PR01349 WNTPROTEIN
PRINTS; PR01841 WNT1PROTEIN; PR01842 WNT2PROTEIN; PR01843 WNT3PROTEIN
PRINTS; PR01845 WNT6PROTEIN; PR01891 WNT7PROTEIN; PR01892 WNT8PROTEIN
PRINTS; PR01893 WNT10PROTEIN; PR01894 WNT14PROTEIN; PR01895 WNT16PROTEIN
MIM; 603490
Creation date 11-MAR-2003
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.BRAIN RES.REV. 33 1-12 (2000).
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
7. GAVIN, B.J., MCMAHON, J.A. AND MCMAHON, A.P.
Expression of multiple novel Wnt-1/int-1-related genes during fetal and
adult mouse development.
GENES DEV. 4 2319-2332 (1990).
8. MCMAHON, J.A., MCMAHON, A.P. AND WEINBERG, R.A.
Essential function of Wnt-4 in mammary gland development downstream of
progesterone signaling.
GENES DEV. 14 650-654 (2000).
9. STARK, K., VAINIO, S., VASSILEVA, G. AND MCMAHON, A.P.
Epithelial transformation of metanephric mesenchyme in the developing
kidney regulated by Wnt-4.
NATURE 372 679-683 (1994).
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidneys,
regulation of mammary gland proliferation and Alzheimer's disease [3,4].
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been
identified in vertebrates, with multiple subtypes within some classes.
Wnt-4 cDNA was isolated from mouse using a PCR-based strategy, where it was
found to be expressed in adult tissues, particularly in brain and lung [7].
Wnt-4 is believed to act downstream of progesterone signalling to play
an important role in mammary gland development [8]. Furthermore, mutations
in the Wnt-4 gene have been linked to kidney defects [9].
WNT4PROTEIN is a 4-element fingerprint that provides a signature for Wnt-4
proteins. The fingerprint was derived from an initial alignment of 7
sequences: the motifs were drawn from conserved regions spanning the N-
terminal half of the alignment, focusing on those sections that characterise
Wnt-4 proteins but distinguish them from related Wnt subtypes - motifs 1 and
2 lie between the putative signal peptide and the first conserved cysteine;
and motifs 3 and 4 reside in regions between other well-conserved N-terminal
cysteines. Two iterations on SPTR40_20f were required to reach convergence,
at which point a true set comprising 9 sequences was identified.
SUMMARY INFORMATION
9 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 9 9 9 9
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
Q96T81 WNT4_MOUSE WNT4_HUMAN WNT4_CHICK
WNT4_RAT WNT4_XENLA WNT4_BRARE WNT4_DROME
Q9VM29
PROTEIN TITLES
Q96T81 SIGNALING PROTEIN WNT-4 - Homo sapiens (Human).
WNT4_MOUSE WNT-4 protein precursor - Mus musculus (Mouse).
WNT4_HUMAN WNT-4 protein precursor - Homo sapiens (Human).
WNT4_CHICK WNT-4 protein precursor - Gallus gallus (Chicken).
WNT4_RAT WNT-4 protein precursor - Rattus norvegicus (Rat).
WNT4_XENLA WNT-4 protein precursor (XWNT-4) - Xenopus laevis (African c
WNT4_BRARE WNT-4 protein precursor - Brachydanio rerio (Zebrafish) (Zeb
WNT4_DROME WNT-4 protein precursor (DWNT-4) - Drosophila melanogaster (
Q9VM29 WNT4 PROTEIN - Drosophila melanogaster (Fruit fly).
SCAN HISTORY
SPTR40_20f 2 300 NSINGLE
INITIAL MOTIF SETS
WNT4PROTEIN1 Length of motif = 11 Motif number = 1
Wnt-4 protein motif I - 1
PCODE ST INT
YLAKLSSVGSI WNT4_XENLA 27 27
YLAKLSSVGSI WNT4_RAT 27 27
YLAKLSSVGSI WNT4_HUMAN 27 27
YLAKLSSVGSI WNT4_MOUSE 27 27
YGGTMLNGGGV WNT4_DROME 32 32
YGGTMLNGGGV Q9VM29 32 32
YLAKLSSVGSI WNT4_CHICK 27 27
WNT4PROTEIN2 Length of motif = 13 Motif number = 2
Wnt-4 protein motif II - 1
PCODE ST INT
SEEETCEKLKGPI WNT4_XENLA 38 0
SEEETCEKLKGLI WNT4_RAT 38 0
SEEETCEKLKGLI WNT4_HUMAN 38 0
SEEETCEKLKGLI WNT4_MOUSE 38 0
SNTNNMDMQQGLY WNT4_DROME 54 11
SNTNNMDMQQGLY Q9VM29 54 11
SEEETCEKLKGLI WNT4_CHICK 38 0
WNT4PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-4 protein motif III - 1
PCODE ST INT
RRGAQLAIEE WNT4_XENLA 68 17
RHGAQLAIEE WNT4_RAT 68 17
RRGAQLAIEE WNT4_HUMAN 68 17
RRGAQLAIEE WNT4_MOUSE 68 17
RRLATTHCEE WNT4_DROME 117 50
RRLATTHCEE Q9VM29 117 50
RRGAQLAIEE WNT4_CHICK 68 17
WNT4PROTEIN4 Length of motif = 12 Motif number = 4
Wnt-4 protein motif IV - 1
PCODE ST INT
TLDTLPVFGKVV WNT4_XENLA 91 13
TLDSLPVFGKVV WNT4_RAT 91 13
TLDSLPVFGKVV WNT4_HUMAN 91 13
TLDSLPVFGKVV WNT4_MOUSE 91 13
TRGKRNIFKKLY WNT4_DROME 139 12
TRGKRNIFKKLY Q9VM29 139 12
TLDTLPVFGKVV WNT4_CHICK 91 13
FINAL MOTIF SETS
WNT4PROTEIN1 Length of motif = 11 Motif number = 1
Wnt-4 protein motif I - 2
PCODE ST INT
YLAKLSSVGSI Q96T81 27 27
YLAKLSSVGSI WNT4_MOUSE 27 27
YLAKLSSVGSI WNT4_HUMAN 27 27
YLAKLSSVGSI WNT4_CHICK 27 27
YLAKLSSVGSI WNT4_RAT 27 27
YLAKLSSVGSI WNT4_XENLA 27 27
YLAKLSSVGSI WNT4_BRARE 27 27
YGGTMLNGGGV Q9VM29 32 32
YGGTMLNGGGV WNT4_DROME 32 32
WNT4PROTEIN2 Length of motif = 13 Motif number = 2
Wnt-4 protein motif II - 2
PCODE ST INT
SEEETCEKLKGLI Q96T81 38 0
SEEETCEKLKGLI WNT4_MOUSE 38 0
SEEETCEKLKGLI WNT4_HUMAN 38 0
SEEETCEKLKGLI WNT4_CHICK 38 0
SEEETCEKLKGLI WNT4_RAT 38 0
SEEETCEKLKGPI WNT4_XENLA 38 0
SDEETCEKLRGLI WNT4_BRARE 38 0
SNTNNMDMQQGLY Q9VM29 54 11
SNTNNMDMQQGLY WNT4_DROME 54 11
WNT4PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-4 protein motif III - 2
PCODE ST INT
RRGAQLAIEE Q96T81 68 17
RRGAQLAIEE WNT4_MOUSE 68 17
RRGAQLAIEE WNT4_HUMAN 68 17
RRGAQLAIEE WNT4_CHICK 68 17
RHGAQLAIEE WNT4_RAT 68 17
RRGAQLAIEE WNT4_XENLA 68 17
RRGAQLAIDE WNT4_BRARE 68 17
RRLATTHCEE Q9VM29 117 50
RRLATTHCEE WNT4_DROME 117 50
WNT4PROTEIN4 Length of motif = 12 Motif number = 4
Wnt-4 protein motif IV - 2
PCODE ST INT
TLDSLPVFGKVV Q96T81 91 13
TLDSLPVFGKVV WNT4_MOUSE 91 13
TLDSLPVFGKVV WNT4_HUMAN 91 13
TLDTLPVFGKVV WNT4_CHICK 91 13
TLDSLPVFGKVV WNT4_RAT 91 13
TLDTLPVFGKVV WNT4_XENLA 91 13
TLESVPVFGKVV WNT4_BRARE 91 13
TRGKRNIFKKLY Q9VM29 139 12
TRGKRNIFKKLY WNT4_DROME 139 12
User query: Display/Full Code "WNT4PROTEIN"