WORKLIST ENTRIES (1):
WNT3PROTEIN View alignment Wnt-3 protein signature
Type of fingerprint: COMPOUND with 3 elements
Links:
PRINTS; PR01349 WNTPROTEIN
PRINTS; PR01841 WNT1PROTEIN; PR01842 WNT2PROTEIN; PR01844 WNT4PROTEIN
PRINTS; PR01845 WNT6PROTEIN; PR01891 WNT7PROTEIN; PR01892 WNT8PROTEIN
PRINTS; PR01893 WNT10PROTEIN; PR01894 WNT14PROTEIN; PR01895 WNT16PROTEIN
MIM; 165330
Creation date 06-JAN-2003
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.BRAIN RES.REV. 33 1-12 (2000).
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
7. ROELINK, H., WAGENAAR, E., SILVA, S.L.D. AND NUSSE, R.
Wnt-3, a gene activated by proviral insertion in mouse mammary tumors,
is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and
adult brain.
PROC.NATL.ACAD.SCI.U.S.A. 87 4519-4523 (1990).
8. ROELINK, H., WANG, J., BLACK, D.M., SOLOMON, E. AND NUSSE, R.
Molecular cloning and chromosomal localization to 17q21 of the human WNT3
gene.
GENOMICS 17 790-792 (1993).
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidneys,
regulation of mammary gland proliferation and Alzheimer's disease [3,4].
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been
identified in vertebrates, with multiple subtypes within some classes.
The Wnt-3 gene was first identified in mouse in 1987, where it was found to
be expressed during embryogenesis and in adult brain. Insertion of proviral
DNA from mouse mammary tumour virus (MMTV) occurs at the mouse Wnt-3 locus,
leading to activation of the gene [7]. However, the human Wnt-3 gene was not
found to be amplified or rearranged in breast cancer tissues [8].
WNT3PROTEIN is a 3-element fingerprint that provides a signature for
Wnt-3 proteins. The fingerprint was derived from an initial alignment of 2
sequences: the motifs were drawn from conserved regions spanning the C-
terminal half of the alignment, focusing on those sections that characterise
Wnt-3 proteins but distinguish them from related Wnt subtypes - the motifs
reside between a number of highly conserved cysteines. Two iterations on
SPTR40_20f were required to reach convergence, at which point a true set
comprising 6 sequences was identified. A single partial match was also
found, Q8WS76, a putative WNT3 protein from Branchiostoma floridae (Florida
lancelet) that fails to make a significant match with motif 2.
SUMMARY INFORMATION
6 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
3| 6 6 6
2| 1 0 1
--+----------------
| 1 2 3
True positives..
WNT3_HUMAN WNT3_MOUSE Q9PWH1 WN3A_XENLA
WN3A_MOUSE Q969P2
Subfamily: Codes involving 2 elements
Subfamily True positives..
Q8WS76
PROTEIN TITLES
WNT3_HUMAN WNT-3 proto-oncogene protein precursor - Homo sapiens (Human
WNT3_MOUSE WNT-3 proto-oncogene protein precursor - Mus musculus (Mouse
Q9PWH1 WNT-3A - Gallus gallus (Chicken).
WN3A_XENLA WNT-3A protein precursor (XWNT-3A) - Xenopus laevis (African
WN3A_MOUSE WNT-3A protein precursor - Mus musculus (Mouse).
Q969P2 CDNA FLJ31716 FIS, CLONE NT2RI2006565, HIGHLY SIMILAR TO WNT
Q8WS76 SECRETED GLYCOPROTEIN WNT3 - Branchiostoma floridae (Florida
SCAN HISTORY
SPTR40_20f 2 300 NSINGLE
INITIAL MOTIF SETS
WNT3PROTEIN1 Length of motif = 10 Motif number = 1
Wnt-3 protein motif I - 1
PCODE ST INT
SHHKGPPGEG WNT3_HUMAN 143 143
SHHKGPPGEG WNT3_MOUSE 143 143
WNT3PROTEIN2 Length of motif = 13 Motif number = 2
Wnt-3 protein motif II - 1
PCODE ST INT
ADARENRPDARSA WNT3_HUMAN 173 20
ADARENRPDARSA WNT3_MOUSE 173 20
WNT3PROTEIN3 Length of motif = 12 Motif number = 3
Wnt-3 protein motif III - 1
PCODE ST INT
GHNTRTEKRKEK WNT3_HUMAN 318 132
GHNTRTEKRKEK WNT3_MOUSE 318 132
FINAL MOTIF SETS
WNT3PROTEIN1 Length of motif = 10 Motif number = 1
Wnt-3 protein motif I - 2
PCODE ST INT
SHHKGPPGEG WNT3_HUMAN 143 143
SHHKGPPGEG WNT3_MOUSE 143 143
TRHKGSPGEG Q9PWH1 164 164
THHKGPPGEG WN3A_XENLA 140 140
SRLQGSPGEG WN3A_MOUSE 140 140
SRHQGSPGKG Q969P2 140 140
WNT3PROTEIN2 Length of motif = 13 Motif number = 2
Wnt-3 protein motif II - 2
PCODE ST INT
ADARENRPDARSA WNT3_HUMAN 173 20
ADARENRPDARSA WNT3_MOUSE 173 20
ADARENRPDARSA Q9PWH1 194 20
ADARENRPDARSA WN3A_XENLA 170 20
ADARENRPDARSA WN3A_MOUSE 170 20
ADARENRPDARSA Q969P2 170 20
WNT3PROTEIN3 Length of motif = 12 Motif number = 3
Wnt-3 protein motif III - 2
PCODE ST INT
GHNTRTEKRKEK WNT3_HUMAN 318 132
GHNTRTEKRKEK WNT3_MOUSE 318 132
GHNTRTEKRKEK Q9PWH1 339 132
GQNTRTEKRKEK WN3A_XENLA 315 132
GHNARTERRREK WN3A_MOUSE 315 132
GHNARAERRREK Q969P2 315 132
User query: Display/Full Code "WNT3PROTEIN"