WORKLIST ENTRIES (1):
WNT2PROTEIN View alignment Wnt-2 protein signature
Type of fingerprint: COMPOUND with 3 elements
Links:
PRINTS; PR01349 WNTPROTEIN
PRINTS; PR01841 WNT1PROTEIN; PR01843 WNT3PROTEIN; PR01844 WNT4PROTEIN
PRINTS; PR01845 WNT6PROTEIN; PR01891 WNT7PROTEIN; PR01892 WNT8PROTEIN
PRINTS; PR01893 WNT10PROTEIN; PR01894 WNT14PROTEIN; PR01895 WNT16PROTEIN
MIM; 147870
Creation date 07-JAN-2003
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.BRAIN RES.REV. 33 1-12 (2000).
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
7. WAINWRIGHT, B.J., SCAMBLER, P.J., STANIER, P., WATSON, E.K., BELL, G.,
WICKING, C., ESTIVILL, X., COURTNEY, M., BOUE, A. AND PEDERSEN, P.S.
Isolation of a human gene with protein sequence similarity to human and
murine int-1 and the Drosophila segment polarity mutant wingless.
EMBO J. 7 1743-1748 (1988).
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidneys,
regulation of mammary gland proliferation and Alzheimer's disease [3,4].
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been
identified in vertebrates, with multiple subtypes within some classes.
The Wnt-2 gene was isolated from a human lung cDNA library in 1988 [7]. The
protein was initially named IRP for Int-1 Related Protein and later termed
Wnt-2. Northern blot analysis from a range of embryonic and adult tissues
revealed that the gene was expressed almost exclusively in placenta and lung.
Mutation of the mouse Wnt-2 gene leads to placental defects.
WNT2PROTEIN is a 3-element fingerprint that provides a signature for Wnt-2
proteins. The fingerprint was derived from an initial alignment of 3
sequences: the motifs were drawn from conserved regions spanning the N-
terminal half of the alignment, focusing on those sections that characterise
Wnt-2 proteins but distinguish them from related Wnt subtypes - motif 1
includes the first conserved cysteine; and motif 3 lies in the central
portion of the protein. Two iterations on SPTR40_20f were required to reach
convergence, at which point a true set comprising 9 sequences was identified.
SUMMARY INFORMATION
9 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
3| 9 9 9
2| 0 0 0
--+----------------
| 1 2 3
True positives..
WNT2_MOUSE WNT2_HUMAN Q9CZW3 Q98SN7
WN2B_HUMAN WN2B_MOUSE WN2B_XENLA Q8UW39
WNT2_BRARE
PROTEIN TITLES
WNT2_MOUSE WNT-2 protein precursor (IRP protein) (INT-1 related protein
WNT2_HUMAN WNT-2 protein precursor (IRP protein) (Int-1 related protein
Q9CZW3 2610510E18RIK PROTEIN - Mus musculus (Mouse).
Q98SN7 WG/INT-1 RELATED GENE PRODUCT WNT-2B - Gallus gallus (Chicke
WN2B_HUMAN WNT-2B protein precursor (WNT-13) - Homo sapiens (Human).
WN2B_MOUSE WNT-2B protein precursor (WNT-13) - Mus musculus (Mouse).
WN2B_XENLA WNT-2B protein precursor (Xwnt-2b) - Xenopus laevis (African
Q8UW39 WNT2 PROTEIN - Fugu rubripes (Japanese pufferfish) (Takifugu
WNT2_BRARE WNT-2 protein precursor - Brachydanio rerio (Zebrafish) (Zeb
SCAN HISTORY
SPTR40_20f 2 300 NSINGLE
INITIAL MOTIF SETS
WNT2PROTEIN1 Length of motif = 12 Motif number = 1
Wnt-2 protein motif I - 1
PCODE ST INT
SQVMCDNIPGLI WNT2_BRARE 35 35
SRVMCDNVPGLV WNT2_HUMAN 37 37
SRVMCDNVPGLV WNT2_MOUSE 37 37
WNT2PROTEIN2 Length of motif = 11 Motif number = 2
Wnt-2 protein motif II - 1
PCODE ST INT
TMAREHNLFGR WNT2_BRARE 87 40
TLDRDHSLFGR WNT2_HUMAN 89 40
TLDRDHSLFGR WNT2_MOUSE 89 40
WNT2PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-2 protein motif III - 1
PCODE ST INT
RKERDARALM WNT2_BRARE 175 77
RKGKDARALM WNT2_HUMAN 177 77
RKGKDARALM WNT2_MOUSE 177 77
FINAL MOTIF SETS
WNT2PROTEIN1 Length of motif = 12 Motif number = 1
Wnt-2 protein motif I - 2
PCODE ST INT
SRVMCDNVPGLV Q9CZW3 37 37
SRVMCDNVPGLV WNT2_HUMAN 37 37
SRVMCDNVPGLV WNT2_MOUSE 37 37
ARVICDNIPGLV Q98SN7 62 62
ARVICDNIPGLV WN2B_HUMAN 68 68
ARVICDNIPGLV WN2B_MOUSE 66 66
ARVICDNIPGLV WN2B_XENLA 28 28
SQVMCDNIPGLV Q8UW39 35 35
SQVMCDNIPGLI WNT2_BRARE 35 35
WNT2PROTEIN2 Length of motif = 11 Motif number = 2
Wnt-2 protein motif II - 2
PCODE ST INT
TLDRDHSLFGR Q9CZW3 89 40
TLDRDHSLFGR WNT2_HUMAN 89 40
TLDRDHSLFGR WNT2_MOUSE 89 40
TLDRDHTVFGR Q98SN7 114 40
TLDRDHTVFGR WN2B_HUMAN 120 40
TLDRDHTVFGR WN2B_MOUSE 118 40
TLDRDHTVFGR WN2B_XENLA 80 40
TTARDHNLFGR Q8UW39 87 40
TMAREHNLFGR WNT2_BRARE 87 40
WNT2PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-2 protein motif III - 2
PCODE ST INT
RKGKDARALM Q9CZW3 177 77
RKGKDARALM WNT2_HUMAN 177 77
RKGKDARALM WNT2_MOUSE 177 77
KKVKDARALM Q98SN7 202 77
KRLKDARALM WN2B_HUMAN 208 77
KRLKDARALM WN2B_MOUSE 206 77
KRLKDARALM WN2B_XENLA 168 77
RKERDARALM Q8UW39 175 77
RKERDARALM WNT2_BRARE 175 77
User query: Display/Full Code "WNT2PROTEIN"