WORKLIST ENTRIES (1):
WNT1PROTEIN View alignment Wnt-1 protein signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR01349 WNTPROTEIN
PRINTS; PR01842 WNT2PROTEIN; PR01843 WNT3PROTEIN; PR01844 WNT4PROTEIN
PRINTS; PR01845 WNT6PROTEIN; PR01891 WNT7PROTEIN; PR01892 WNT8PROTEIN
PRINTS; PR01893 WNT10PROTEIN; PR01894 WNT14PROTEIN; PR01895 WNT16PROTEIN
MIM; 164820
Creation date 06-JAN-2003
1. WODARZ, A. AND NUSSE, R.
Mechanisms of Wnt signal transduction.
ANNU.REV.CELL DEV.BIOL. 14 59-88 (1998).
2. BEJSOVEC, A.
Signal transduction: Wnt signalling shows its versatility.
CURR.BIOL. 9 R684-R687 (1999).
3. DE FERRARI, G.V. AND INESTROSA, N.C.
Wnt signaling function in Alzheimer's disease.
BRAIN RES.BRAIN RES.REV. 33 1-12 (2000).
4. SEMENOV, M.V. AND SNYDER, M.
Human dishevelled genes constitute a DHR-containing multigene family.
GENOMICS 42 302-310 (1997).
5. PEIFER, M. AND POLAKIS, P.
Wnt signalling in oncogenesis and embryogenesis - a look outside the
nucleus.
SCIENCE 287 1606-1609 (2000).
6. MOON, R.T.
An introduction to non-canonical Wnt and Frizzled signaling.
SEMIN.CELL DEV.BIOL. 13 215 (2002).
Wnt proteins constitute a large family of secreted molecules that are
involved in intercellular signalling during development. The name derives
from the first 2 members of the family to be discovered: int-1 (mouse) and
wingless (Drosophila) [1]. It is now recognised that Wnt signalling controls
many cell fate decisions in a variety of different organisms, including
mammals [2]. Wnt signalling has been implicated in tumorigenesis, early
mesodermal patterning of the embryo, morphogenesis of the brain and kidneys,
regulation of mammary gland proliferation and Alzheimer's disease [3,4].
Wnt-mediated signalling is believed to proceed initially through binding to
cell surface receptors of the frizzled family; the signal is subsequently
transduced through several cytoplasmic components to B-catenin, which enters
the nucleus and activates the transcription of several genes important in
development [5]. More recently, however, several non-canonical Wnt
signalling pathways have been elucidated that act independently of
B-catenin [6]. Members of the Wnt gene family are defined by their sequence
similarity to mouse Wnt-1 and Wingless in Drosophila. They encode proteins
of ~350-400 residues in length, with orthologues identified in several,
mostly vertebrate, species. Very little is known about the structure of
Wnts as they are notoriously insoluble; in terms of primary structure, the
family is characterised by a signal sequence and an almost invariant pattern
of 23-24 conserved cysteines [1]. Fifteen major Wnt gene families have been
identified in vertebrates, with multiple subtypes within some classes.
The Wnt-1 gene was first identified in 1982 as a proto-oncogene activated by
the integration of mouse mammary tumour virus (MMTV) in mammary tumours.
With the identification of Drosophila wingless, however, it became clear
that Wnt genes are important regulators of many developmental decisions.
Mutation of the embryonic mouse Wnt-1 gene leads to loss of the midbrain and
cerebellum; and a number of processes including segment polarity and limb
development are interrupted in Drosophila wingless mutants [1].
WNT1PROTEIN is a 5-element fingerprint that provides a signature for Wnt-1
proteins. The fingerprint was derived from an initial alignment of 5
sequences: the motifs were drawn from conserved regions spanning the full
alignment length, focusing on those sections that characterise Wnt-1
proteins but distinguish them from related Wnt subtypes - motifs 1 and 2 lie
towards the N-terminus, between the putative signal peptide and the first
conserved cysteine residue; and motifs 3-5 reside between conserved
cysteines in the C-terminal portion. Two iterations on SPTR40_20f were
required to reach convergence, at which point a true set comprising 6
sequences was identified.
SUMMARY INFORMATION
6 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 6 6 6 6 6
4| 0 0 0 0 0
3| 0 0 0 0 0
2| 0 0 0 0 0
--+--------------------------
| 1 2 3 4 5
True positives..
WNT1_BRARE P79752 WNT1_AMBME WNT1_HUMAN
WNT1_MOUSE WNT1_XENLA
PROTEIN TITLES
WNT1_BRARE WNT-1 protein precursor - Brachydanio rerio (Zebrafish) (Zeb
P79752 WNT1 - Fugu rubripes (Japanese pufferfish) (Takifugu rubripe
WNT1_AMBME WNT-1 protein precursor - Ambystoma mexicanum (Axolotl).
WNT1_HUMAN WNT-1 proto-oncogene protein precursor - Homo sapiens (Human
WNT1_MOUSE WNT-1 proto-oncogene protein precursor - Mus musculus (Mouse
WNT1_XENLA WNT-1 protein precursor (XWNT-1) (XINT-1) - Xenopus laevis (
SCAN HISTORY
SPTR40_20f 2 300 NSINGLE
INITIAL MOTIF SETS
WNT1PROTEIN1 Length of motif = 11 Motif number = 1
Wnt-1 protein motif I - 1
PCODE ST INT
AANSSGRWWGI WNT1_HUMAN 27 27
AVNNSGRWWGI WNT1_BRARE 26 26
AVNNSGRWWGV WNT1_AMBME 26 26
AVNNSGKWWGI WNT1_XENLA 26 26
AANSSGRWWGI WNT1_MOUSE 27 27
WNT1PROTEIN2 Length of motif = 12 Motif number = 2
Wnt-1 protein motif II - 1
PCODE ST INT
SKSLQLVLEPSL WNT1_HUMAN 50 12
SKNVQLVLDPSL WNT1_BRARE 49 12
TKNVQLVLDPSL WNT1_AMBME 49 12
ARPVPLVLDPSL WNT1_XENLA 51 14
SKSLQLVLEPSL WNT1_MOUSE 50 12
WNT1PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-1 protein motif III - 1
PCODE ST INT
SGEKGRDLRF WNT1_HUMAN 187 125
SSERGRDLRY WNT1_BRARE 186 125
SSERGRDLRY WNT1_AMBME 186 125
SSERGRDLKY WNT1_XENLA 187 124
SGEKGRDLRF WNT1_MOUSE 187 125
WNT1PROTEIN4 Length of motif = 11 Motif number = 4
Wnt-1 protein motif IV - 1
PCODE ST INT
RAELLRLEPED WNT1_HUMAN 268 71
RADPRHLEPEN WNT1_BRARE 268 72
RVQTHHLEPEN WNT1_AMBME 267 71
RSDPPHLEPEN WNT1_XENLA 269 72
RAELLRLEPED WNT1_MOUSE 268 71
WNT1PROTEIN5 Length of motif = 10 Motif number = 5
Wnt-1 protein motif V - 1
PCODE ST INT
RLGTAGTAGR WNT1_HUMAN 304 25
KTGTHGTSGR WNT1_BRARE 304 25
KTGTSGTSGR WNT1_AMBME 303 25
KNGTPGTTGR WNT1_XENLA 305 25
RLGTAGTAGR WNT1_MOUSE 304 25
FINAL MOTIF SETS
WNT1PROTEIN1 Length of motif = 11 Motif number = 1
Wnt-1 protein motif I - 2
PCODE ST INT
AVNNSGRWWGI WNT1_BRARE 26 26
AVNNSGRWWGI P79752 26 26
AVNNSGRWWGV WNT1_AMBME 26 26
AANSSGRWWGI WNT1_HUMAN 27 27
AANSSGRWWGI WNT1_MOUSE 27 27
AVNNSGKWWGI WNT1_XENLA 26 26
WNT1PROTEIN2 Length of motif = 12 Motif number = 2
Wnt-1 protein motif II - 2
PCODE ST INT
SKNVQLVLDPSL WNT1_BRARE 49 12
SKNVQLVLDPSL P79752 49 12
TKNVQLVLDPSL WNT1_AMBME 49 12
SKSLQLVLEPSL WNT1_HUMAN 50 12
SKSLQLVLEPSL WNT1_MOUSE 50 12
ARPVPLVLDPSL WNT1_XENLA 51 14
WNT1PROTEIN3 Length of motif = 10 Motif number = 3
Wnt-1 protein motif III - 2
PCODE ST INT
SSERGRDLRY WNT1_BRARE 186 125
SSERGRDLRY P79752 186 125
SSERGRDLRY WNT1_AMBME 186 125
SGEKGRDLRF WNT1_HUMAN 187 125
SGEKGRDLRF WNT1_MOUSE 187 125
SSERGRDLKY WNT1_XENLA 187 124
WNT1PROTEIN4 Length of motif = 11 Motif number = 4
Wnt-1 protein motif IV - 2
PCODE ST INT
RADPRHLEPEN WNT1_BRARE 268 72
RAHPRHLEPEN P79752 268 72
RVQTHHLEPEN WNT1_AMBME 267 71
RAELLRLEPED WNT1_HUMAN 268 71
RAELLRLEPED WNT1_MOUSE 268 71
RSDPPHLEPEN WNT1_XENLA 269 72
WNT1PROTEIN5 Length of motif = 10 Motif number = 5
Wnt-1 protein motif V - 2
PCODE ST INT
KTGTHGTSGR WNT1_BRARE 304 25
KTGTLGTSGR P79752 304 25
KTGTSGTSGR WNT1_AMBME 303 25
RLGTAGTAGR WNT1_HUMAN 304 25
RLGTAGTAGR WNT1_MOUSE 304 25
KNGTPGTTGR WNT1_XENLA 305 25
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