WORKLIST ENTRIES (1):
VASOPRSNV2R View alignment View Structure Vasopressin V2 receptor signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00896 VASOPRESSINR
PRINTS; PR00752 VASOPRSNV1AR; PR00897 VASOPRSNV1BR; PR00665 OXYTOCINR
INTERPRO; IPR000161
Creation date 15-APR-1998; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Vasopressin and oxytocin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.284-291.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Vasopressin and oxytocin are members of the neurohypophyseal hormone family
found in all mammalian species [6]. They are present in high levels in the
posterior pituitary. Vasopressin has an essential role in the control of
the water content of the body, acting in the kidney to increase water and
sodium absorption [6]. In higher concentrations, vasopressin stimulates
contraction of vascular smooth muscle, stimulates glycogen breakdown in the
liver, induces platelet activation, and evokes release of corticotrophin
from the anterior pituitary [6]. Vasopressin and its analogues are used
clinically to treat diabetes insipidus [6].
The V2 receptor is found in high levels in the osmoregulatory epithelia of
the terminal urinary tract, where it stimulates water reabsorption [6]. It
is also present in lower levels in the endothelium and blood vessels of some
species, where it induces vasodilation [6]. In the CNS, binding sites are
found in the subiculum, with lower levels in caudate-putamen and islands
of Calleja [6]. The receptor is involved in an effector pathway that forms
cAMP through activation of Gs [6].
VASOPRSNV2R is an 8-element fingerprint that provides a signature for
vasopressin V2 receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from short conserved
sections spanning the full alignment length, focusing on those regions
that characterise the vasopressin V2 receptors but distinguish them from
the rest of the vasopressin family - motifs 1 and 2 reside at the N-terminus;
motif 3 spans the first cytoplasmic loop; motif 4 spans the second
cytoplasmic loop; motifs 5 and 6 span the third cytoplasmic loop; and
motifs 7 and 8 reside at the C-terminus. A single iteration on OWL30.1 was
required to reach convergence, no further sequences being identified
beyond the starting set.
An update on SPTR37_9f identified a true set of 6 sequences, and 1
partial match.
SUMMARY INFORMATION
6 codes involving 8 elements
0 codes involving 7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 6 6 6 6 6 6 6 6
7| 0 0 0 0 0 0 0 0
6| 1 1 1 1 1 1 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
O88721 V2R_RAT V2R_PIG V2R_HUMAN
O77808 V2R_BOVIN
Subfamily: Codes involving 6 elements
Subfamily True positives..
O43192
PROTEIN TITLES
O88721 VASOPRESSIN RECEPTOR TYPE II - MUS MUSCULUS (MOUSE).
V2R_RAT VASOPRESSIN V2 RECEPTOR (RENAL-TYPE ARGININE VASOPRESSIN REC
V2R_PIG VASOPRESSIN V2 RECEPTOR (RENAL-TYPE ARGININE VASOPRESSIN REC
V2R_HUMAN VASOPRESSIN V2 RECEPTOR (RENAL-TYPE ARGININE VASOPRESSIN REC
O77808 VASOPRESSIN V2 RECEPTOR - CANIS FAMILIARIS (DOG).
V2R_BOVIN VASOPRESSIN V2 RECEPTOR (RENAL-TYPE ARGININE VASOPRESSIN REC
O43192 VASOPRESSIN RECEPTOR TYPE 2 - HOMO SAPIENS (HUMAN).
SCAN HISTORY
OWL30_1 1 50 NSINGLE
SPTR37_9f 2 7 NSINGLE
INITIAL MOTIF SETS
VASOPRSNV2R1 Length of motif = 17 Motif number = 1
Vasopressin V2 receptor motif I - 1
PCODE ST INT
TTSAVPGHPSLPSLPSN V2R_HUMAN 6 6
TTSAVPRALSWPAAPGN V2R_PIG 5 5
TTSAVPWHLSQPTPAGN V2R_BOVIN 6 6
TVSAVPGLFSPPSSPSN V2R_RAT 6 6
VASOPRSNV2R2 Length of motif = 15 Motif number = 2
Vasopressin V2 receptor motif II - 1
PCODE ST INT
NSSQERPLDTRDPLL V2R_HUMAN 22 -1
NGSEREPLDDRDPLL V2R_PIG 21 -1
NGSEGELLTARDPLL V2R_BOVIN 22 -1
NSSQEELLDDRDPLL V2R_RAT 22 -1
VASOPRSNV2R3 Length of motif = 16 Motif number = 3
Vasopressin V2 receptor motif III - 1
PCODE ST INT
ALARRGRRGHWAPIHV V2R_HUMAN 61 24
ALVRRGRRGRWAPMHV V2R_PIG 60 24
ALVRRGRRGRWAPMHV V2R_BOVIN 61 24
ALIRRGRRGRWAPMHV V2R_RAT 61 24
VASOPRSNV2R4 Length of motif = 16 Motif number = 4
Vasopressin V2 receptor motif IV - 1
PCODE ST INT
MLAYRHGSGAHWNRPV V2R_HUMAN 145 68
MLAYRHGGGARWNRPV V2R_PIG 144 68
MLAHRHGGGTHWNRPV V2R_BOVIN 145 68
MLAYRHGGGARWNRPV V2R_RAT 145 68
VASOPRSNV2R5 Length of motif = 16 Motif number = 5
Vasopressin V2 receptor motif V - 1
PCODE ST INT
QVLIFREIHASLVPGP V2R_HUMAN 225 64
QVLIFREIHTSLVPGP V2R_PIG 224 64
QVLIFREIHASLGPGP V2R_BOVIN 224 63
QVLIFREIHASLVPGP V2R_RAT 225 64
VASOPRSNV2R6 Length of motif = 20 Motif number = 6
Vasopressin V2 receptor motif VI - 1
PCODE ST INT
RPGGRRRGRRTGSPGEGAHV V2R_HUMAN 243 2
RAGGHRGGRRAGSPREGARV V2R_PIG 242 2
RAGGPRRGCRPGSPAEGARV V2R_BOVIN 242 2
RAGRRRRGRRTGSPSEGAHV V2R_RAT 243 2
VASOPRSNV2R7 Length of motif = 15 Motif number = 7
Vasopressin V2 receptor motif VII - 1
PCODE ST INT
ASFSSSVSSELRSLL V2R_HUMAN 326 63
ASFSSSISSELRSLL V2R_PIG 325 63
ASFSSSISSELRSLL V2R_BOVIN 325 63
ASFSSSVSSELRSLL V2R_RAT 326 63
VASOPRSNV2R8 Length of motif = 20 Motif number = 8
Vasopressin V2 receptor motif VIII - 1
PCODE ST INT
SLGPQDESCTTASSSLAKDT V2R_HUMAN 350 9
SLRPQEESCATASSFSARDT V2R_PIG 349 9
SPGPQEESCATASSFLAKDT V2R_BOVIN 349 9
SLGPQDESCATASSSLMKDT V2R_RAT 350 9
FINAL MOTIF SETS
VASOPRSNV2R1 Length of motif = 17 Motif number = 1
Vasopressin V2 receptor motif I - 2
PCODE ST INT
TTSAVPGALSSPSSPSN O88721 6 6
TVSAVPGLFSPPSSPSN V2R_RAT 6 6
TTSAVPRALSWPAAPGN V2R_PIG 5 5
TTSAVPGHPSLPSLPSN V2R_HUMAN 6 6
TTSAVPRTLSPPTPAGN O77808 6 6
TTSAVPWHLSQPTPAGN V2R_BOVIN 6 6
VASOPRSNV2R2 Length of motif = 15 Motif number = 2
Vasopressin V2 receptor motif II - 2
PCODE ST INT
NSSQEELLDDRDPLL O88721 22 -1
NSSQEELLDDRDPLL V2R_RAT 22 -1
NGSEREPLDDRDPLL V2R_PIG 21 -1
NSSQERPLDTRDPLL V2R_HUMAN 22 -1
GNGSRELLDTRDPLL O77808 21 -2
NGSEGELLTARDPLL V2R_BOVIN 22 -1
VASOPRSNV2R3 Length of motif = 16 Motif number = 3
Vasopressin V2 receptor motif III - 2
PCODE ST INT
ALIRRGRRGRWAPMHV O88721 61 24
ALIRRGRRGRWAPMHV V2R_RAT 61 24
ALVRRGRRGRWAPMHV V2R_PIG 60 24
ALARRGRRGHWAPIHV V2R_HUMAN 61 24
ALARRVRRGRWAPMHV O77808 60 24
ALVRRGRRGRWAPMHV V2R_BOVIN 61 24
VASOPRSNV2R4 Length of motif = 16 Motif number = 4
Vasopressin V2 receptor motif IV - 2
PCODE ST INT
MLAYRHGGGARWNRPV O88721 145 68
MLAYRHGGGARWNRPV V2R_RAT 145 68
MLAYRHGGGARWNRPV V2R_PIG 144 68
MLAYRHGSGAHWNRPV V2R_HUMAN 145 68
MLAYRHGGGARWNRPV O77808 144 68
MLAHRHGGGTHWNRPV V2R_BOVIN 145 68
VASOPRSNV2R5 Length of motif = 16 Motif number = 5
Vasopressin V2 receptor motif V - 2
PCODE ST INT
QVLIFREIHASLVPGP O88721 225 64
QVLIFREIHASLVPGP V2R_RAT 225 64
QVLIFREIHTSLVPGP V2R_PIG 224 64
QVLIFREIHASLVPGP V2R_HUMAN 225 64
QVLIFREIHSSLVPGP O77808 224 64
QVLIFREIHASLGPGP V2R_BOVIN 224 63
VASOPRSNV2R6 Length of motif = 20 Motif number = 6
Vasopressin V2 receptor motif VI - 2
PCODE ST INT
RAGRRRRGHRTGSPSEGAHV O88721 243 2
RAGRRRRGRRTGSPSEGAHV V2R_RAT 243 2
RAGGHRGGRRAGSPREGARV V2R_PIG 242 2
RPGGRRRGRRTGSPGEGAHV V2R_HUMAN 243 2
RAGGCRGGHRTGSPSEGARV O77808 242 2
RAGGPRRGCRPGSPAEGARV V2R_BOVIN 242 2
VASOPRSNV2R7 Length of motif = 15 Motif number = 7
Vasopressin V2 receptor motif VII - 2
PCODE ST INT
ASFSSSVSSELRSLL O88721 326 63
ASFSSSVSSELRSLL V2R_RAT 326 63
ASFSSSISSELRSLL V2R_PIG 325 63
ASFSSSVSSELRSLL V2R_HUMAN 326 63
AFFSSSVSSELRSLF O77808 325 63
ASFSSSISSELRSLL V2R_BOVIN 325 63
VASOPRSNV2R8 Length of motif = 20 Motif number = 8
Vasopressin V2 receptor motif VIII - 2
PCODE ST INT
SLGPQDESCATASSSLMKDT O88721 350 9
SLGPQDESCATASSSLMKDT V2R_RAT 350 9
SLRPQEESCATASSFSARDT V2R_PIG 349 9
SLGPQDESCTTASSSLAKDT V2R_HUMAN 350 9
SLGPQEESCATASSFLAKDT O77808 349 9
SPGPQEESCATASSFLAKDT V2R_BOVIN 349 9
User query: Display/Full Code "VASOPRSNV2R"