WORKLIST ENTRIES (1):
SCYTLDPTASE View alignment Scytalidopepsin B aspartic protease (A4) signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR00792 PEPSIN; PR00731 CAULIMOPTASE; PR00863 NODAVIRPTASE
PRINTS; PR00781 LIPOSIGPTASE; PR00920 SPUMVIRPTASE
INTERPRO; IPR000250
Creation date 10-SEP-1998; UPDATE 07-JUN-1999
1. RAWLINGS, N.D. AND BARRETT A.J.
Families of aspartic peptidases, and those of unkown catalytic mechanism.
METHODS ENZYMOL. 248 183-228 (1995).
2. RAWLINGS, N.D. AND BARRETT, A.J.
Evolutionary families of peptidases.
BIOCHEM.J. 290 205-218 (1993).
3. RAWLINGS, N.D. AND BARRETT, A.J.
MEROPS - Peptidase Database
http://www.bi.bbsrc.ac.uk/merops/merops.htm
4. RAWLINGS, N.D. AND BARRETT, A.J.
Family A4 - Scytalidopepsin B aspartic peptidases
http://www.bi.bbsrc.ac.uk/merops/famcards/a4.htm
5. INOUE, H., KIMURA, T., MAKABE, O. AND TAKAHASHI, K.
The gene and deduced protein sequences of the zymogen of Aspergillus niger
acid proteinase A.
J.BIOL.CHEM. 266 19484-19489 (1991).
Aspartic proteases have a catalytic aspartate residue at their active site.
A number of families (denoted by the letter A followed by a number) have
been identified, most of which (including pepsin, retropepsin and
cauliflower mosaic virus peptidase) have been grouped into a single clan
(AA), as they are all thought to share a common ancestry. Families A6 and
A21 belong to the AB clan. The remaining families have not yet received
clan assignments [1-3].
Scytalidopepsin B is a fungal aspartic protease belonging to the A4 family
[1,4]. This family of proteases has not yet been assigned to a clan. The
proteins are thermostable, pepstatin insensitive and are active at low pH
ranges [1]. The enzyme has a unique heterodimeric structure, with a
39-residue light chain and a 173-residue heavy chain bound to each other
non-covalently [5].
SCYTLDPTASE is a 5-element fingerprint that provides a signature for the
scytalidopepsin B aspartic protease (A4) family. The fingerprint was
derived from an initial alignment of 4 sequences: the motifs were drawn
from conserved regions spanning virtually the full length of the heavy
chain. A single iteration on OWL30.2 was required to reach convergence
at which point a true set comprising 5 sequences was identified.
An update on SPTR37_9f identified a true set of 4 sequences.
SUMMARY INFORMATION
4 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 4 4 4 4 4
4| 0 0 0 0 0
3| 0 0 0 0 0
2| 0 0 0 0 0
--+--------------------------
| 1 2 3 4 5
True positives..
PRTA_ASPNG Q00551 Q00550 PRTB_SCYLI
PROTEIN TITLES
PRTA_ASPNG ASPERGILLOPEPSIN II PRECURSOR (EC 3.4.23.19) (ACID PROTEASE
Q00551 ACID PROTEINASE - CRYPHONECTRIA PARASITICA (CHESNUT BLIGHT F
Q00550 ACID PROTEINASE - CRYPHONECTRIA PARASITICA (CHESNUT BLIGHT F
PRTB_SCYLI SCYTALIDOPEPSIN B (EC 3.4.23.32) (ACID PROTEASE B) (SLB) - S
SCAN HISTORY
OWL30_2 1 50 NSINGLE
SPTR37_9f 2 5 NSINGLE
INITIAL MOTIF SETS
SCYTLDPTASE1 Length of motif = 23 Motif number = 1
Scytalidopepsin B aspartic protease motif I - 1
PCODE ST INT
ASAWVGIDGDTCETAILQTGVDF PRTA_ASPNG 116 116
SAAWVGIDGDTCQTAILQTGFDW PRTB_SCYLI 36 36
ASAWVGIDGDTAQNSILQTGVDF S63631 104 104
ASAWVGIDGDTCTSAILQTGVDF S63630 108 108
SCYTLDPTASE2 Length of motif = 21 Motif number = 2
Scytalidopepsin B aspartic protease motif II - 1
PCODE ST INT
YDAWYEWYPDYAYDFSDITIS PRTA_ASPNG 147 8
YDAWYEWYPEVSDDFSGITIS PRTB_SCYLI 64 5
YDAWYEWYPDYAYDFSGISIS S63631 135 8
FDAWYEWYPNYAYDFSGFSVS S63630 139 8
SCYTLDPTASE3 Length of motif = 18 Motif number = 3
Scytalidopepsin B aspartic protease motif III - 1
PCODE ST INT
GDSIKVTVEATSKSSGSA PRTA_ASPNG 169 1
GDSIQMSVTATSDTSGSA PRTB_SCYLI 86 1
GDTIKVTVTATTTTSGTA S63631 157 1
GDTIVMTASASSLKAGTV S63630 161 1
SCYTLDPTASE4 Length of motif = 14 Motif number = 4
Scytalidopepsin B aspartic protease motif IV - 1
PCODE ST INT
ENLTTGQSVTHTFS PRTA_ASPNG 189 2
ENLTTGQKVSKSFS PRTB_SCYLI 106 2
ENVTKGTTVTHTFT S63631 177 2
ENSTTGKKVTQSFS S63630 181 2
SCYTLDPTASE5 Length of motif = 16 Motif number = 5
Scytalidopepsin B aspartic protease motif V - 1
PCODE ST INT
LCETNAEWIVEDFESG PRTA_ASPNG 209 6
LCRTNAEFIIEDFEEC PRTB_SCYLI 125 5
LQELNAEWIVEDFEEG S63631 196 5
LCEYNAEWIVEDFESG S63630 200 5
FINAL MOTIF SETS
SCYTLDPTASE1 Length of motif = 23 Motif number = 1
Scytalidopepsin B aspartic protease motif I - 2
PCODE ST INT
ASAWVGIDGDTCETAILQTGVDF PRTA_ASPNG 116 116
ASAWVGIDGDTAQNSILQTGVDF Q00551 104 104
ASAWVGIDGDTCTSAILQTGVDF Q00550 108 108
SAAWVGIDGDTCQTAILQTGFDW PRTB_SCYLI 36 36
SCYTLDPTASE2 Length of motif = 21 Motif number = 2
Scytalidopepsin B aspartic protease motif II - 2
PCODE ST INT
YDAWYEWYPDYAYDFSDITIS PRTA_ASPNG 147 8
YDAWYEWYPDYAYDFSGISIS Q00551 135 8
FDAWYEWYPNYAYDFSGFSVS Q00550 139 8
YDAWYEWYPEVSDDFSGITIS PRTB_SCYLI 64 5
SCYTLDPTASE3 Length of motif = 18 Motif number = 3
Scytalidopepsin B aspartic protease motif III - 2
PCODE ST INT
GDSIKVTVEATSKSSGSA PRTA_ASPNG 169 1
GDTIKVTVTATTTTSGTA Q00551 157 1
GDTIVMTASASSLKAGTV Q00550 161 1
GDSIQMSVTATSDTSGSA PRTB_SCYLI 86 1
SCYTLDPTASE4 Length of motif = 14 Motif number = 4
Scytalidopepsin B aspartic protease motif IV - 2
PCODE ST INT
ENLTTGQSVTHTFS PRTA_ASPNG 189 2
ENVTKGTTVTHTFT Q00551 177 2
ENSTTGKKVTQSFS Q00550 181 2
ENLTTGQKVSKSFS PRTB_SCYLI 106 2
SCYTLDPTASE5 Length of motif = 16 Motif number = 5
Scytalidopepsin B aspartic protease motif V - 2
PCODE ST INT
LCETNAEWIVEDFESG PRTA_ASPNG 209 6
LQELNAEWIVEDFEEG Q00551 196 5
LCEYNAEWIVEDFESG Q00550 200 5
LCRTNAEFIIEDFEEC PRTB_SCYLI 125 5
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