WORKLIST ENTRIES (1):
PYOCINKILLER View alignment View Structure Pyocin S killer protein signature
Type of fingerprint: COMPOUND with 10 elements
Links:
PRINTS; PR01295 CLOACIN; PR01296 CLOACNIMMNTY; PR01297 LYSISCOLICIN
PRINTS; PR01298 MICROCIN; PR01299 PYOCIN
Creation date 17-FEB-2000
1. SANO, Y., MATSUI, H., KOBAYASHI, M. AND KAGEYAMA, M.
Molecular structures and functions of pyocins S1 and S2 in Pseudomonas
aeruginosa.
J.BACTERIOL. 175 2907-2916 (1993).
Pyocins S1 and S2 are S-type bacteriocins of Pseudomonas aeruginosa with
different receptor recognition specificities [1]. The genetic determinants
of these pyocins have been cloned from the NIH-H and PAO chromosomes of
P.aeruginosa [1]. The determinants each constitute an operon that encodes
two proteins of molecular weight 65,600 and 10,000 (pyocin S1) or 74,000 and
10,000 (pyocin S2) with a characteristic sequence (P box), a possible
regulatory element involved in the induction of pyocin production, in the
5' upstream region [1]. These pyocins have almost identical sequences,
except in the N-terminal portions of the large proteins, which are
substantially different. This similarity suggests that S1 and S2 pyocins,
like pyocin AP41, originated from a common ancestor of the E2 group colicins
[1]. Purified pyocins S1 and S2 constitute a complex of the two proteins.
Both pyocins cause breakdown of chromosomal DNA and complete inhibition of
lipid synthesis in sensitive cells [1]. The large protein (not the complex),
shows in vitro DNase activity [1]. This activity is inhibited by the small
protein of either pyocin [1].
PYOCINKILLER is a 10-element fingerprint that provides a signature for the
large component of pyocin S killer proteins. The fingerprint was derived
from an initial alignment of 2 sequences: the motifs were drawn from
conserved regions spanning the C-terminal half of the alignment. A single
iteration on SPTR37_10f was required to reach convergence, no further
sequences being identified beyond the starting set. Four partial matches
were found, including the large component of pyocin AP41 and related
colicins, all of which match the C-terminal motifs.
SUMMARY INFORMATION
2 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
3 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
10| 2 2 2 2 2 2 2 2 2 2
9| 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 1 1 1 0 1
3| 0 0 0 0 0 0 3 3 0 3
2| 0 0 0 0 0 0 0 0 0 0
--+---------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10
True positives..
PYS1_PSEAE PYS2_PSEAE
Subfamily: Codes involving 4 elements
Subfamily True positives..
Q51502
Subfamily: Codes involving 3 elements
Subfamily True positives..
CEA2_ECOLI Q51604 CEA9_ECOLI
PROTEIN TITLES
PYS1_PSEAE PYOCIN S1 (EC 3.1.21.1) (KILLER PROTEIN) - PSEUDOMONAS AERUG
PYS2_PSEAE PYOCIN S2 (EC 3.1.21.1) (KILLER PROTEIN) - PSEUDOMONAS AERUG
Q51502 THE LARGE COMPONENT OF PYOCIN AP41 - PSEUDOMONAS AERUGINOSA.
CEA2_ECOLI COLICIN E2 (EC 3.1.21.1) - ESCHERICHIA COLI.
Q51604 COLICIN E7 - ESCHERICHIA COLI.
CEA9_ECOLI COLICIN E9 (EC 3.1.21.1) - ESCHERICHIA COLI.
SCAN HISTORY
SPTR37_10f 1 50 NSINGLE
INITIAL MOTIF SETS
PYOCINKILLER1 Length of motif = 22 Motif number = 1
Pyocin S killer protein motif I - 1
PCODE ST INT
AAIRAANTYAMPANGSVVATAA PYS1_PSEAE 242 242
AAIRAANTYAMPANGSVVATAA PYS2_PSEAE 313 313
PYOCINKILLER2 Length of motif = 21 Motif number = 2
Pyocin S killer protein motif II - 1
PCODE ST INT
QVAQGAASLAQAISDAIAVLG PYS1_PSEAE 269 5
QVAQGAASLAQAISDAIAVLG PYS2_PSEAE 340 5
PYOCINKILLER3 Length of motif = 19 Motif number = 3
Pyocin S killer protein motif III - 1
PCODE ST INT
RVLASAPSVMAVGFASLTY PYS1_PSEAE 290 0
RVLASAPSVMAVGFASLTY PYS2_PSEAE 361 0
PYOCINKILLER4 Length of motif = 24 Motif number = 4
Pyocin S killer protein motif IV - 1
PCODE ST INT
RYALGMDAAKLGLPPSVNLNAVAK PYS1_PSEAE 325 16
RYALGMDAAKLGLPPSVNLNAVAK PYS2_PSEAE 396 16
PYOCINKILLER5 Length of motif = 19 Motif number = 5
Pyocin S killer protein motif V - 1
PCODE ST INT
DGVSVPKAVPVRMAAYNAT PYS1_PSEAE 376 27
DGVSVPKAVPVRMAAYNAT PYS2_PSEAE 447 27
PYOCINKILLER6 Length of motif = 20 Motif number = 6
Pyocin S killer protein motif VI - 1
PCODE ST INT
IYVMFRDPRDVPGAATGKGQ PYS1_PSEAE 479 84
IYVMFRDPRDVPGAATGKGQ PYS2_PSEAE 550 84
PYOCINKILLER7 Length of motif = 22 Motif number = 7
Pyocin S killer protein motif VII - 1
PCODE ST INT
EGAPIPSQIADKLRGKTFKNWR PYS1_PSEAE 512 13
EGAPIPSQIADKLRGKTFKNWR PYS2_PSEAE 583 13
PYOCINKILLER8 Length of motif = 20 Motif number = 8
Pyocin S killer protein motif VIII - 1
PCODE ST INT
DFREQFWIAVANDPELSKQF PYS1_PSEAE 534 0
DFREQFWIAVANDPELSKQF PYS2_PSEAE 606 1
PYOCINKILLER9 Length of motif = 18 Motif number = 9
Pyocin S killer protein motif IX - 1
PCODE ST INT
SLAVMRDGGAPYVRESEQ PYS1_PSEAE 557 3
SLAVMRDGGAPYVRESEQ PYS2_PSEAE 629 3
PYOCINKILLER10 Length of motif = 20 Motif number = 10
Pyocin S killer protein motif X - 1
PCODE ST INT
VYNMGNLVAVTPKRHIEIHK PYS1_PSEAE 595 20
VYNMGNLVAVTPKRHIEIHK PYS2_PSEAE 667 20
FINAL MOTIF SETS
PYOCINKILLER1 Length of motif = 22 Motif number = 1
Pyocin S killer protein motif I - 1
PCODE ST INT
AAIRAANTYAMPANGSVVATAA PYS1_PSEAE 242 242
AAIRAANTYAMPANGSVVATAA PYS2_PSEAE 313 313
PYOCINKILLER2 Length of motif = 21 Motif number = 2
Pyocin S killer protein motif II - 1
PCODE ST INT
QVAQGAASLAQAISDAIAVLG PYS1_PSEAE 269 5
QVAQGAASLAQAISDAIAVLG PYS2_PSEAE 340 5
PYOCINKILLER3 Length of motif = 19 Motif number = 3
Pyocin S killer protein motif III - 1
PCODE ST INT
RVLASAPSVMAVGFASLTY PYS1_PSEAE 290 0
RVLASAPSVMAVGFASLTY PYS2_PSEAE 361 0
PYOCINKILLER4 Length of motif = 24 Motif number = 4
Pyocin S killer protein motif IV - 1
PCODE ST INT
RYALGMDAAKLGLPPSVNLNAVAK PYS1_PSEAE 325 16
RYALGMDAAKLGLPPSVNLNAVAK PYS2_PSEAE 396 16
PYOCINKILLER5 Length of motif = 19 Motif number = 5
Pyocin S killer protein motif V - 1
PCODE ST INT
DGVSVPKAVPVRMAAYNAT PYS1_PSEAE 376 27
DGVSVPKAVPVRMAAYNAT PYS2_PSEAE 447 27
PYOCINKILLER6 Length of motif = 20 Motif number = 6
Pyocin S killer protein motif VI - 1
PCODE ST INT
IYVMFRDPRDVPGAATGKGQ PYS1_PSEAE 479 84
IYVMFRDPRDVPGAATGKGQ PYS2_PSEAE 550 84
PYOCINKILLER7 Length of motif = 22 Motif number = 7
Pyocin S killer protein motif VII - 1
PCODE ST INT
EGAPIPSQIADKLRGKTFKNWR PYS1_PSEAE 512 13
EGAPIPSQIADKLRGKTFKNWR PYS2_PSEAE 583 13
PYOCINKILLER8 Length of motif = 20 Motif number = 8
Pyocin S killer protein motif VIII - 1
PCODE ST INT
DFREQFWIAVANDPELSKQF PYS1_PSEAE 534 0
DFREQFWIAVANDPELSKQF PYS2_PSEAE 606 1
PYOCINKILLER9 Length of motif = 18 Motif number = 9
Pyocin S killer protein motif IX - 1
PCODE ST INT
SLAVMRDGGAPYVRESEQ PYS1_PSEAE 557 3
SLAVMRDGGAPYVRESEQ PYS2_PSEAE 629 3
PYOCINKILLER10 Length of motif = 20 Motif number = 10
Pyocin S killer protein motif X - 1
PCODE ST INT
VYNMGNLVAVTPKRHIEIHK PYS1_PSEAE 595 20
VYNMGNLVAVTPKRHIEIHK PYS2_PSEAE 667 20
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