WORKLIST ENTRIES (1):
PSYCHOSINER View alignment View Structure Psychosine receptor signature
Type of fingerprint: COMPOUND with 10 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
Creation date 18-DEC-2001
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. IM, D.S., HEISE, C.E., NGUYEN, T., O'DOWD, B.F. AND LYNCH, K.R.
Identification of a molecular target of psychosine and its role in globoid
cell formation.
J.CELL BIOL. 153(2) 429-434 (2001).
G-protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine,
para-crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Psychosine is a glycosphingolipid implicated in the pathology of globoid
cell leukodystrophy (GLD), a hereditary metabolic disorder that results from
the absence of the enzyme galactosyl ceramide. This deficiency results in
the accumulation of psychosine in the brain, leading to apoptosis of
oligodendrocytes, progressive demyelination and the existence of large,
multinuclear cells (globoid cells) derived from microglia [6].
The molecular mechanism by which these toxic effects might be mediated has
recently been elucidated by the identification of TDAG8, an orphan G
protein-coupled receptor, as a receptor for psychosine [6]. TDAG8 is
expressed at high levels in the spleen, peripheral blood leukocytes, lymph
nodes and lung. Activation of the receptor in RH7777 hepatoma cells by
psychosine and related lysoglycolipids results in a pertussis toxin-
insensitive inhibition of forskolin-induced cAMP accummulation, possibly
through coupling to Gz proteins [6].
PSYCHOSINER is a 10-element fingerprint that provides a signature for the
psychosine receptor. The fingerprint was derived from an initial alignment
of 2 sequences: the motifs were drawn from conserved sections within N- and
C-terminal and loop regions, focusing on those areas of the alignment that
characterise the psychosine receptors but distinguish them from the rest of
the rhodopsin-like superfamily - motif 1 resides at the N-terminus; motif 2
spans the first cytoplasmic loop; motif 3 encodes the first external loop;
motif 4 spans the second cytoplasmic loop; motifs 5 and 6 reside in the
second external loop; motif 7 lies in the third cytoplasmic loop; motif 8
resides in the C-terminal portion of the third external loop; and motifs 9
and 10 lie at the C-terminus. A single iteration on SPTR40_18f was required
to reach convergence, no further sequences being identified beyond the
starting set.
SUMMARY INFORMATION
2 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
10| 2 2 2 2 2 2 2 2 2 2
9| 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0 0
--+---------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10
True positives..
O75819 TDA8_MOUSE
PROTEIN TITLES
O75819 T CELL-DEATH ASSOCIATED PROTEIN - Homo sapiens (Human).
TDA8_MOUSE Probable G protein-coupled receptor TDAG8 - Mus musculus (Mo
SCAN HISTORY
SPTR40_18f 1 100 NSINGLE
INITIAL MOTIF SETS
PSYCHOSINER1 Length of motif = 18 Motif number = 1
Psychosine receptor motif I - 1
PCODE ST INT
MNSMCIEEQRHLEHYLFP TDA8_MOUSE 3 3
MNSTCIEEQHDLDHYLFP O75819 1 1
PSYCHOSINER2 Length of motif = 14 Motif number = 2
Psychosine receptor motif II - 1
PCODE ST INT
CVSFLQAKKENELG TDA8_MOUSE 39 18
CVSFLQPKKESELG O75819 37 18
PSYCHOSINER3 Length of motif = 23 Motif number = 3
Psychosine receptor motif III - 1
PCODE ST INT
WINYTWNKDNWTFSPTLCKGSVF TDA8_MOUSE 72 19
WIDYTWNKDNWTFSPALCKGSAF O75819 70 19
PSYCHOSINER4 Length of motif = 13 Motif number = 4
Psychosine receptor motif IV - 1
PCODE ST INT
VYPLKFSFLRTRR TDA8_MOUSE 119 24
VYPLKFFFLRTRR O75819 117 24
PSYCHOSINER5 Length of motif = 20 Motif number = 5
Psychosine receptor motif V - 1
PCODE ST INT
WKDETSVEYCDSDKSNFTLC TDA8_MOUSE 153 21
WEDETVVEYCDAEKSNFTLC O75819 151 21
PSYCHOSINER6 Length of motif = 19 Motif number = 6
Psychosine receptor motif VI - 1
PCODE ST INT
YDKYPLEKWQINLNLFRTC TDA8_MOUSE 173 0
YDKYPLEKWQINLNLFRTC O75819 171 0
PSYCHOSINER7 Length of motif = 15 Motif number = 7
Psychosine receptor motif VII - 1
PCODE ST INT
AVRHNQATENSEKRR TDA8_MOUSE 211 19
AVRHNKATENKEKKR O75819 209 19
PSYCHOSINER8 Length of motif = 12 Motif number = 8
Psychosine receptor motif VIII - 1
PCODE ST INT
SGWQTFTVYRVT TDA8_MOUSE 264 38
SGKRTYTMYRIT O75819 264 40
PSYCHOSINER9 Length of motif = 16 Motif number = 9
Psychosine receptor motif IX - 1
PCODE ST INT
TETGRADMWNILKLCT TDA8_MOUSE 294 18
TETGRYDMWNILKFCT O75819 294 18
PSYCHOSINER10 Length of motif = 16 Motif number = 10
Psychosine receptor motif X - 1
PCODE ST INT
ILSVSTRDAVELEIID TDA8_MOUSE 322 12
ILSVSTKDTMELEVLE O75819 322 12
FINAL MOTIF SETS
PSYCHOSINER1 Length of motif = 18 Motif number = 1
Psychosine receptor motif I - 1
PCODE ST INT
MNSTCIEEQHDLDHYLFP O75819 1 1
MNSMCIEEQRHLEHYLFP TDA8_MOUSE 3 3
PSYCHOSINER2 Length of motif = 14 Motif number = 2
Psychosine receptor motif II - 1
PCODE ST INT
CVSFLQPKKESELG O75819 37 18
CVSFLQAKKENELG TDA8_MOUSE 39 18
PSYCHOSINER3 Length of motif = 23 Motif number = 3
Psychosine receptor motif III - 1
PCODE ST INT
WIDYTWNKDNWTFSPALCKGSAF O75819 70 19
WINYTWNKDNWTFSPTLCKGSVF TDA8_MOUSE 72 19
PSYCHOSINER4 Length of motif = 13 Motif number = 4
Psychosine receptor motif IV - 1
PCODE ST INT
VYPLKFFFLRTRR O75819 117 24
VYPLKFSFLRTRR TDA8_MOUSE 119 24
PSYCHOSINER5 Length of motif = 20 Motif number = 5
Psychosine receptor motif V - 1
PCODE ST INT
WEDETVVEYCDAEKSNFTLC O75819 151 21
WKDETSVEYCDSDKSNFTLC TDA8_MOUSE 153 21
PSYCHOSINER6 Length of motif = 19 Motif number = 6
Psychosine receptor motif VI - 1
PCODE ST INT
YDKYPLEKWQINLNLFRTC O75819 171 0
YDKYPLEKWQINLNLFRTC TDA8_MOUSE 173 0
PSYCHOSINER7 Length of motif = 15 Motif number = 7
Psychosine receptor motif VII - 1
PCODE ST INT
AVRHNKATENKEKKR O75819 209 19
AVRHNQATENSEKRR TDA8_MOUSE 211 19
PSYCHOSINER8 Length of motif = 12 Motif number = 8
Psychosine receptor motif VIII - 1
PCODE ST INT
SGKRTYTMYRIT O75819 264 40
SGWQTFTVYRVT TDA8_MOUSE 264 38
PSYCHOSINER9 Length of motif = 16 Motif number = 9
Psychosine receptor motif IX - 1
PCODE ST INT
TETGRYDMWNILKFCT O75819 294 18
TETGRADMWNILKLCT TDA8_MOUSE 294 18
PSYCHOSINER10 Length of motif = 16 Motif number = 10
Psychosine receptor motif X - 1
PCODE ST INT
ILSVSTKDTMELEVLE O75819 322 12
ILSVSTRDAVELEIID TDA8_MOUSE 322 12
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