WORKLIST ENTRIES (1):
PEROPSIN View alignment View Structure Peropsin signature
Type of fingerprint: COMPOUND with 11 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00238 OPSIN; PR00574 OPSINBLUE; PR00575 OPSINREDGRN
PRINTS; PR00576 OPSINRH1RH2; PR00577 OPSINRH3RH4; PR00578 OPSINLTRLEYE
PRINTS; PR00666 PINOPSIN; PR00579 RHODOPSIN; PR00239 RHODOPSNTAIL
PRINTS; PR00667 RPERETINALR
INTERPRO; IPR002962
Creation date 14-OCT-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
3. APPLEBURY, M.L. AND HARGRAVE, P.A.
Molecular biology of the visual pigments.
VISION RES. 26(12) 1881-1895 (1986).
4. FRYXELL, K.J. AND MEYEROWITZ, E.M.
The evolution of rhodopsins and neurotransmitter receptors.
J.MOL.EVOL. 33(4) 367-378 (1991).
5. SUN, H., GILBERT, D.J., COPELAND, N.G., JENKINS, N.A. AND NATHANS, J.
Peropsin, a novel visual pigment-like protein located in the apical
microvilli of the retinal pigment epithelium.
PROC.NATL.ACAD.SCI.U.S.A. 94 9893-9898 (1997).
Opsins are integral membrane proteins that belong to a superfamily of G-
protein-coupled receptors (GPCRs). The activating ligands of the different
superfamily members vary widely in structure and character, yet the proteins
appear faithfully to have conserved a basic structural framework, believed
to consist of 7 transmembrane (TM) helices. Although the sequences of these
proteins are very diverse, reflecting to some extent this broad range of
activating ligands, nevertheless, motifs have been identified in the TM
regions that are characteristic of virtually the entire superfamily [1,2].
Amongst the exceptions are the olfactory receptors, which cluster together
in a subfamily, which lacks significant matches with domains 2, 4 and 6.
Interestingly, the opsins also seem to be emerging as increasingly atypical
of the superfamily, clustering most strongly, in phylogenetic analyses, with
the olfactory receptors [2].
Visual pigments are the light-absorbing molecules that mediate vision [3,4].
They comprise an apoprotein (opsin), covalently linked to the chromophore
cis-retinal. Vision is effected through the absorption of a photon by the
chromophore, which is isomerised to the all-trans form, promoting a
conformational change in the protein.
Peropsin is a visual pigment-like protein found in ocular tissues [5].
The protein has been localised to the apical face of the retinal pigment
epithelium (RPE), most prominently to the microvilli that surround the
photoreceptor outer segments [5]. It is believed that peropsin may play a
role in RPE physiology, either by detecting light directly, or by monitoring
the concentration of retinoids or other photoreceptor-derived compounds [5].
PEROPSIN is an 11-element fingerprint that provides a signature for
peropsins. The fingerprint was derived from an initial alignment of 2
sequences: the motifs were drawn from conserved regions spanning the
full alignment length, focusing on those sections that characterise the
peropsins but distinguish them from the rest of the opsin family - motif 1
lies at the N-terminus, leading into TM domain 1; motif 2 spans the first
cytoplasmic loop; motifs 3 and 4 span the C-terminal portion of TM domain 2
and the first external loop; motif 5 encodes the N-terminal portion of TM
domain 3; motif 6 lies in the second cytoplasmic loop; motif 7 encodes the
N-terminal portion of TM domain 5; motif 8 resides in the third cytoplasmic
loop; motif 9 spans the third external loop and the N-terminal portion of
TM domain 7; and motifs 10 and 11 reside at the C-terminus. A single
iteration on SPTR37_10f was required to reach convergnence, no further
sequences being identified beyond the starting set.
SUMMARY INFORMATION
2 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
11| 2 2 2 2 2 2 2 2 2 2 2
10| 0 0 0 0 0 0 0 0 0 0 0
9| 0 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0 0 0
--+--------------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10 11
True positives..
OPSX_HUMAN OPSX_MOUSE
PROTEIN TITLES
OPSX_HUMAN VISUAL PIGMENT-LIKE RECEPTOR PEROPSIN - HOMO SAPIENS (HUMAN)
OPSX_MOUSE VISUAL PIGMENT-LIKE RECEPTOR PEROPSIN - MUS MUSCULUS (MOUSE)
SCAN HISTORY
SPTR37_10f 1 100 NSINGLE
INITIAL MOTIF SETS
PEROPSIN1 Length of motif = 15 Motif number = 1
Peropsin motif I - 1
PCODE ST INT
GSVFSQTEHNIVATY OPSX_HUMAN 17 17
GSVFSRTEHSVIAAY OPSX_MOUSE 17 17
PEROPSIN2 Length of motif = 12 Motif number = 2
Peropsin motif II - 1
PCODE ST INT
VLGIFIKYKELR OPSX_HUMAN 46 14
VLGIFIKYKELR OPSX_MOUSE 46 14
PEROPSIN3 Length of motif = 16 Motif number = 3
Peropsin motif III - 1
PCODE ST INT
TDIGVSSIGYPMSAAS OPSX_HUMAN 70 12
TDIGVSSIGYPMSAAS OPSX_MOUSE 70 12
PEROPSIN4 Length of motif = 12 Motif number = 4
Peropsin motif IV - 1
PCODE ST INT
DLYGSWKFGYAG OPSX_HUMAN 86 0
DLHGSWKFGHAG OPSX_MOUSE 86 0
PEROPSIN5 Length of motif = 17 Motif number = 5
Peropsin motif V - 1
PCODE ST INT
YAGLNIFFGMASIGLLT OPSX_HUMAN 101 3
YAGLNIFFGMVSIGLLT OPSX_MOUSE 101 3
PEROPSIN6 Length of motif = 16 Motif number = 6
Peropsin motif VI - 1
PCODE ST INT
TICLPDVGRRMTTNTY OPSX_HUMAN 126 8
TISCPDVGRRMTTNTY OPSX_MOUSE 126 8
PEROPSIN7 Length of motif = 15 Motif number = 7
Peropsin motif VII - 1
PCODE ST INT
FVSYTMTVIAINFIV OPSX_HUMAN 186 44
FVSYTMMVIVVNFIV OPSX_MOUSE 186 44
PEROPSIN8 Length of motif = 15 Motif number = 8
Peropsin motif VIII - 1
PCODE ST INT
DCTESLNRDWSDQID OPSX_HUMAN 223 22
DCTAHLHRDWADQAD OPSX_MOUSE 223 22
PEROPSIN9 Length of motif = 19 Motif number = 9
Peropsin motif IX - 1
PCODE ST INT
WASFGDPKKIPPPMAIIAP OPSX_HUMAN 262 24
WACFGNPKKIPPSMAIIAP OPSX_MOUSE 262 24
PEROPSIN10 Length of motif = 15 Motif number = 10
Peropsin motif X - 1
PCODE ST INT
AMLAMFKCQTHQTMP OPSX_HUMAN 304 23
AMLAMFKCQPHLAVP OPSX_MOUSE 304 23
PEROPSIN11 Length of motif = 20 Motif number = 11
Peropsin motif XI - 1
PCODE ST INT
PVTSILPMDVSQNPLASGRI OPSX_HUMAN 318 -1
PEPSTLPMDMPQSSLAPVRI OPSX_MOUSE 318 -1
FINAL MOTIF SETS
PEROPSIN1 Length of motif = 15 Motif number = 1
Peropsin motif I - 1
PCODE ST INT
GSVFSQTEHNIVATY OPSX_HUMAN 17 17
GSVFSRTEHSVIAAY OPSX_MOUSE 17 17
PEROPSIN2 Length of motif = 12 Motif number = 2
Peropsin motif II - 1
PCODE ST INT
VLGIFIKYKELR OPSX_HUMAN 46 14
VLGIFIKYKELR OPSX_MOUSE 46 14
PEROPSIN3 Length of motif = 16 Motif number = 3
Peropsin motif III - 1
PCODE ST INT
TDIGVSSIGYPMSAAS OPSX_HUMAN 70 12
TDIGVSSIGYPMSAAS OPSX_MOUSE 70 12
PEROPSIN4 Length of motif = 12 Motif number = 4
Peropsin motif IV - 1
PCODE ST INT
DLYGSWKFGYAG OPSX_HUMAN 86 0
DLHGSWKFGHAG OPSX_MOUSE 86 0
PEROPSIN5 Length of motif = 17 Motif number = 5
Peropsin motif V - 1
PCODE ST INT
YAGLNIFFGMASIGLLT OPSX_HUMAN 101 3
YAGLNIFFGMVSIGLLT OPSX_MOUSE 101 3
PEROPSIN6 Length of motif = 16 Motif number = 6
Peropsin motif VI - 1
PCODE ST INT
TICLPDVGRRMTTNTY OPSX_HUMAN 126 8
TISCPDVGRRMTTNTY OPSX_MOUSE 126 8
PEROPSIN7 Length of motif = 15 Motif number = 7
Peropsin motif VII - 1
PCODE ST INT
FVSYTMTVIAINFIV OPSX_HUMAN 186 44
FVSYTMMVIVVNFIV OPSX_MOUSE 186 44
PEROPSIN8 Length of motif = 15 Motif number = 8
Peropsin motif VIII - 1
PCODE ST INT
DCTESLNRDWSDQID OPSX_HUMAN 223 22
DCTAHLHRDWADQAD OPSX_MOUSE 223 22
PEROPSIN9 Length of motif = 19 Motif number = 9
Peropsin motif IX - 1
PCODE ST INT
WASFGDPKKIPPPMAIIAP OPSX_HUMAN 262 24
WACFGNPKKIPPSMAIIAP OPSX_MOUSE 262 24
PEROPSIN10 Length of motif = 15 Motif number = 10
Peropsin motif X - 1
PCODE ST INT
AMLAMFKCQTHQTMP OPSX_HUMAN 304 23
AMLAMFKCQPHLAVP OPSX_MOUSE 304 23
PEROPSIN11 Length of motif = 20 Motif number = 11
Peropsin motif XI - 1
PCODE ST INT
PVTSILPMDVSQNPLASGRI OPSX_HUMAN 318 -1
PEPSTLPMDMPQSSLAPVRI OPSX_MOUSE 318 -1
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