WORKLIST ENTRIES (1):
OPSINRH3RH4 View alignment View Structure Opsin RH3/RH4 signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00238 OPSIN; PR00574 OPSINBLUE; PR00575 OPSINREDGRN
PRINTS; PR00576 OPSINRH1RH2; PR00578 OPSINLTRLEYE; PR01244 PEROPSIN
PRINTS; PR00666 PINOPSIN; PR00579 RHODOPSIN; PR00239 RHODOPSNTAIL
PRINTS; PR00667 RPERETINALR
INTERPRO; IPR000856
Creation date 11-SEP-1996; UPDATE 07-JUN-1999
1. APPLEBURY, M.L. AND HARGRAVE, P.A.
Molecular biology of the visual pigments.
VISION RES. 26(12) 1881-1895 (1986).
2. FRYXELL, K.J. AND MEYEROWITZ, E.M.
The evolution of rhodopsins and neurotransmitter receptors.
J.MOL.EVOL. 33(4) 367-378 (1991).
3. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
4. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
5. FRYXELL, K.J. AND MEYEROWITZ, E.M.
An opsin gene that is expressed only in the R7 photoreceptor cell of
Drosophila.
EMBO J. 6(2) 443-451 (1987).
6. ZUKER, C.S., MONTELL, C., JONES, K., LAVERTY, T. AND RUBIN, G.M.J.
A rhodopsin gene expressed in photoreceptor cell R7 of the Drosophila
eye - homologies with other signal-transducing molecules.
NEUROSCIENCE 7(5) 1550-1557 (1987).
7. MONTELL, C., JONES, K., ZUKER, C.S. AND RUBIN, G.M.J.
A second opsin gene expressed in the ultraviolet-sensitive R7
photoreceptor cells of Drosophila melanogaster.
NEUROSCIENCE 7(5) 1558-1566 (1987).
Opsins, the light-absorbing molecules that mediate vision [1,2], are
integral membrane proteins that belong to a superfamily of G protein-coupled
receptors (GPCRs). The activating ligands of the different superfamily
members vary widely in structure and character, yet the proteins appear
faithfully to have conserved a basic structural framework, believed to
consist of 7 transmembrane (TM) helices. Although the sequences of these
proteins are very diverse, reflecting to some extent this broad range of
activating ligands, nevertheless, motifs have been identified in the TM
regions that are characteristic of virtually the entire superfamily [3,4].
Amongst the exceptions are the olfactory receptors, which cluster together
in a subfamily, which lacks significant matches with domains 2, 4 and 6.
Interestingly, the opsins also seem to be emerging as increasingly atypical
of the superfamily, clustering most strongly, in phylogenetic analyses, with
the olfactory receptors [4].
The visual pigments comprise an apoprotein (opsin), covalently linked to
the chromophore 11-cis-retinal. The covalent link is in the form of a
protonated Schiff base between the retinal and a lysine residue located in
TM domain 7. Vision is effected through the absorption of a photon by the
chromophore, which is isomerised to the all-trans form, promoting a
conformational change in the protein.
By contrast with vertebrate rhodopsin, which is found in rod cells, insect
photoreceptors are found in the ommatidia that comprise the compound eyes.
Each Drosophila eye has 800 ommatidia, each of which contains 8 photo-
receptor cells (designated R1-R8): R1-R6 are outer cells, while R7 and R8
are inner cells. Opsins RH3 and RH4 are sensitive to UV light [5-7].
OPSINRH3RH4 is a 7-element fingerprint that provides a signature for the
RH3 and RH4 opsins. The fingerprint was derived from an initial alignment
of 5 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the RH3/RH4 opsins but distinguish them from the rest of
the rhodopsin-like superfamily - motifs 1 and 2 lie at the N-terminus;
motif 3 spans the first external loop; motif 4 lies in the second external
loop; motif 5 spans the C-terminal half of TM domain 5; motif 6 lies in the
the third cytoplasmic loop; and motif 7 lies at the C-terminus. A single
iteration on OWL28.1 was required to reach convergence, no further sequences
being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 8 sequences, and 3
partial matches.
SUMMARY INFORMATION
8 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
1 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 8 8 8 8 8 8 8
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 1 0 0 1 0 1 1
3| 1 0 0 1 0 0 1
2| 0 0 0 1 0 0 1
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
OPS4_DROVI OPS3_DROME OPS4_DROPS OPS3_DROPS
OPS4_DROME O61474 O61473 OPSV_APIME
Subfamily: Codes involving 4 elements
Subfamily True positives..
OPS2_SCHGR
Subfamily: Codes involving 3 elements
Subfamily True positives..
OPSB_APIME
Subfamily: Codes involving 2 elements
Subfamily True positives..
OPS5_DROME
PROTEIN TITLES
OPS4_DROVI OPSIN RH4 (INNER R7 PHOTORECEPTOR CELLS OPSIN) - DROSOPHILA
OPS3_DROME OPSIN RH3 (INNER R7 PHOTORECEPTOR CELLS OPSIN) - DROSOPHILA
OPS4_DROPS OPSIN RH4 (INNER R7 PHOTORECEPTOR CELLS OPSIN) - DROSOPHILA
OPS3_DROPS OPSIN RH3 (INNER R7 PHOTORECEPTOR CELLS OPSIN) - DROSOPHILA
OPS4_DROME OPSIN RH4 (INNER R7 PHOTORECEPTOR CELLS OPSIN) - DROSOPHILA
O61474 SHORT WAVELENGTH-SENSITIVE OPSIN - CAMPONOTUS ABDOMINALIS (C
O61473 SHORT WAVELENGTH-SENSITIVE OPSIN - CATAGLYPHIS BOMBYCINA (SA
OPSV_APIME OPSIN, ULTRAVIOLET-SENSITIVE (AMUVOP) (BUVOPS) - APIS MELLIF
OPS2_SCHGR OPSIN 2 - SCHISTOCERCA GREGARIA (DESERT LOCUST).
OPSB_APIME OPSIN, BLUE-SENSITIVE (AMBLOP) - APIS MELLIFERA (HONEYBEE).
OPS5_DROME OPSIN RH5 - DROSOPHILA MELANOGASTER (FRUIT FLY).
SCAN HISTORY
OWL28_1 1 100 NSINGLE
SPTR37_9f 2 17 NSINGLE
INITIAL MOTIF SETS
OPSINRH3RH41 Length of motif = 17 Motif number = 1
Opsin RH3/RH4 motif I - 1
PCODE ST INT
DLQFLGWNVPPDQIQYI OPS4_DROME 23 23
ELQFLGWNVPPDQIQYI OPS4_DROPS 24 24
DLQFLGWNVPPDQIQHI OPS4_DROVI 27 27
ETRLLGWNVPPEELRHI OPS3_DROME 27 27
ESRLLGWNVPPDELRHI OPS3_DROPS 26 26
OPSINRH3RH42 Length of motif = 17 Motif number = 2
Opsin RH3/RH4 motif II - 1
PCODE ST INT
PEHWLTQLEPPASMHYM OPS4_DROME 40 0
PEHWLTQLEPPASMHYM OPS4_DROPS 41 0
PEHWLTQLEPPASMHYM OPS4_DROVI 44 0
PEHWLTYPEPPESMNYL OPS3_DROME 44 0
PEHWLIYPEPPESMNYL OPS3_DROPS 43 0
OPSINRH3RH43 Length of motif = 12 Motif number = 3
Opsin RH3/RH4 motif III - 1
PCODE ST INT
IFNSFHRGFAIY OPS4_DROME 109 52
IYNSFHRGFALG OPS4_DROPS 112 54
IYNSFHRGFALG OPS4_DROVI 115 54
IYNSFHQGYALG OPS3_DROME 115 54
IYNSFHQGYALG OPS3_DROPS 114 54
OPSINRH3RH44 Length of motif = 15 Motif number = 4
Opsin RH3/RH4 motif IV - 1
PCODE ST INT
YLSDNFDTRLFVGTI OPS4_DROME 207 86
YLSDNFDTRLFVGTI OPS4_DROPS 208 84
YLTDNFDTRLFVGTI OPS4_DROVI 211 84
YLTDNFDTRLFVACI OPS3_DROME 211 84
YLTDNFDTRLFVACI OPS3_DROPS 210 84
OPSINRH3RH45 Length of motif = 18 Motif number = 5
Opsin RH3/RH4 motif V - 1
PCODE ST INT
TLMILYYYSQIVGHVFSH OPS4_DROME 230 8
TLMILYYYSQIVGHVFNH OPS4_DROPS 231 8
TLMIIYYYSQIVGHVFSH OPS4_DROVI 234 8
TTMITYYYSQIVGHVFSH OPS3_DROME 234 8
TTMITYYYSQIVGHVFSH OPS3_DROPS 233 8
OPSINRH3RH46 Length of motif = 15 Motif number = 6
Opsin RH3/RH4 motif VI - 1
PCODE ST INT
SNVDKSKETAEIRIA OPS4_DROME 265 17
SNVDKSKETAEIRIA OPS4_DROPS 266 17
SNVDKSKDTAEIRIA OPS4_DROVI 269 17
SNVDKNKETAEIRIA OPS3_DROME 269 17
SNVDKSKEAAEIRIA OPS3_DROPS 268 17
OPSINRH3RH47 Length of motif = 19 Motif number = 7
Opsin RH3/RH4 motif VII - 1
PCODE ST INT
RLELQKRCPWLGVNEKSGE OPS4_DROME 342 62
RMELQKRCPWLGVNEKSGE OPS4_DROPS 343 62
RMELQKRCPWLAIDEKAPE OPS4_DROVI 346 62
RMELQKRCPWLALNEKAPE OPS3_DROME 346 62
RMELQKRCPWLAISEKAPE OPS3_DROPS 345 62
FINAL MOTIF SETS
OPSINRH3RH41 Length of motif = 17 Motif number = 1
Opsin RH3/RH4 motif I - 2
PCODE ST INT
DLQFLGWNVPPDQIQHI OPS4_DROVI 27 27
ETRLLGWNVPPEELRHI OPS3_DROME 27 27
ELQFLGWNVPPDQIQYI OPS4_DROPS 24 24
ESRLLGWNVPPDELRHI OPS3_DROPS 26 26
DLQFLGWNVPPDQIQYI OPS4_DROME 23 23
PPRLLGWNVPAEELVHI O61474 17 17
PPRLLGWNVPAEELVHI O61473 18 18
PPRLLGWNVPAEELIHI OPSV_APIME 17 17
OPSINRH3RH42 Length of motif = 17 Motif number = 2
Opsin RH3/RH4 motif II - 2
PCODE ST INT
PEHWLTQLEPPASMHYM OPS4_DROVI 44 0
PEHWLTYPEPPESMNYL OPS3_DROME 44 0
PEHWLTQLEPPASMHYM OPS4_DROPS 41 0
PEHWLIYPEPPESMNYL OPS3_DROPS 43 0
PEHWLTQLEPPASMHYM OPS4_DROME 40 0
PEHXLVYPEPNPSLHYL O61474 34 0
PEHWLVYPEPNPSLHYL O61473 35 0
PEHWLVYPEPNPSLHYL OPSV_APIME 34 0
OPSINRH3RH43 Length of motif = 12 Motif number = 3
Opsin RH3/RH4 motif III - 2
PCODE ST INT
IYNSFHRGFALG OPS4_DROVI 115 54
IYNSFHQGYALG OPS3_DROME 115 54
IYNSFHRGFALG OPS4_DROPS 112 54
IYNSFHQGYALG OPS3_DROPS 114 54
IFNSFHRGFAIY OPS4_DROME 109 52
IYNSFHTGFATG O61474 105 54
IYNSFNTGFATG O61473 106 54
IYNSFNTGFALG OPSV_APIME 105 54
OPSINRH3RH44 Length of motif = 15 Motif number = 4
Opsin RH3/RH4 motif IV - 2
PCODE ST INT
YLTDNFDTRLFVGTI OPS4_DROVI 211 84
YLTDNFDTRLFVACI OPS3_DROME 211 84
YLSDNFDTRLFVGTI OPS4_DROPS 208 84
YLTDNFDTRLFVACI OPS3_DROPS 210 84
YLSDNFDTRLFVGTI OPS4_DROME 207 86
YLTDSQEIRYFVPTI O61474 201 84
YLTDTPEIRYFVATI O61473 202 84
YLTDTNEIRIFVATI OPSV_APIME 201 84
OPSINRH3RH45 Length of motif = 18 Motif number = 5
Opsin RH3/RH4 motif V - 2
PCODE ST INT
TLMIIYYYSQIVGHVFSH OPS4_DROVI 234 8
TTMITYYYSQIVGHVFSH OPS3_DROME 234 8
TLMILYYYSQIVGHVFNH OPS4_DROPS 231 8
TTMITYYYSQIVGHVFSH OPS3_DROPS 233 8
TLMILYYYSQIVGHVFSH OPS4_DROME 230 8
MLLIIYYYSQIVGHVVSH O61474 224 8
MSLIIYYYSQIVSHVVNH O61473 225 8
MILIIYYYSQIVSHVVNH OPSV_APIME 224 8
OPSINRH3RH46 Length of motif = 15 Motif number = 6
Opsin RH3/RH4 motif VI - 2
PCODE ST INT
SNVDKSKDTAEIRIA OPS4_DROVI 269 17
SNVDKNKETAEIRIA OPS3_DROME 269 17
SNVDKSKETAEIRIA OPS4_DROPS 266 17
SNVDKSKEAAEIRIA OPS3_DROPS 268 17
SNVDKSKETAEIRIA OPS4_DROME 265 17
SNVNTNAQSAEIRIA O61474 259 17
SNTNTNAQSAEIRIA O61473 260 17
SNANTSSQSAEIRIA OPSV_APIME 259 17
OPSINRH3RH47 Length of motif = 19 Motif number = 7
Opsin RH3/RH4 motif VII - 2
PCODE ST INT
RMELQKRCPWLAIDEKAPE OPS4_DROVI 346 62
RMELQKRCPWLALNEKAPE OPS3_DROME 346 62
RMELQKRCPWLGVNEKSGE OPS4_DROPS 343 62
RMELQKRCPWLAISEKAPE OPS3_DROPS 345 62
RLELQKRCPWLGVNEKSGE OPS4_DROME 342 62
RLELQKRLPWLELQEKPVA O61474 336 62
RLELQKRLPWLELQEKPIE O61473 337 62
RLELQKRLPWLELQEKPIS OPSV_APIME 336 62
User query: Display/Full Code "OPSINRH3RH4"