WORKLIST ENTRIES (1):
OPSINLTRLEYE View alignment View Structure Lateral eye opsin signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00238 OPSIN; PR00574 OPSINBLUE; PR00575 OPSINREDGRN
PRINTS; PR00576 OPSINRH1RH2; PR00577 OPSINRH3RH4; PR01244 PEROPSIN
PRINTS; PR00666 PINOPSIN; PR00579 RHODOPSIN; PR00239 RHODOPSNTAIL
PRINTS; PR00667 RPERETINALR
INTERPRO; IPR001391
Creation date 11-SEP-1996; UPDATE 22-JUN-1999
1. APPLEBURY, M.L. AND HARGRAVE, P.A.
Molecular biology of the visual pigments.
VISION RES. 26(12) 1881-1895 (1986).
2. FRYXELL, K.J. AND MEYEROWITZ, E.M.
The evolution of rhodopsins and neurotransmitter receptors.
J.MOL.EVOL. 33(4) 367-378 (1991).
3. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
4. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
5. WATSON, S. AND ARKINSTALL,
Opsins.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.214-222.
6. SMITH, W.C., PRICE, D.A., GREENBERG, R.M. AND BATTELLE, B.A.
Opsins from the lateral eyes and ocelli of the horseshoe crab, Limulus
polyphemus.
PROC.NATL.ACAD.SCI.U.S.A. 90 6150-6154 (1993).
Opsins, the light-absorbing molecules that mediate vision [1,2], are
integral membrane proteins that belong to a superfamily of G protein-coupled
receptors (GPCRs). The activating ligands of the different superfamily
members vary widely in structure and character, yet the proteins appear
faithfully to have conserved a basic structural framework, believed to
consist of 7 transmembrane (TM) helices. Although the sequences of these
proteins are very diverse, reflecting to some extent this broad range of
activating ligands, nevertheless, motifs have been identified in the TM
regions that are characteristic of virtually the entire superfamily [3,4].
Amongst the exceptions are the olfactory receptors, which cluster together
in a subfamily, which lacks significant matches with domains 2, 4 and 6.
Interestingly, the opsins also seem to be emerging as increasingly atypical
of the superfamily, clustering most strongly, in phylogenetic analyses, with
the olfactory receptors [4].
The visual pigments comprise an apoprotein (opsin), covalently linked to
the chromophore 11-cis-retinal. The covalent link is in the form of a
protonated Schiff base between the retinal and a lysine residue located in
TM domain 7. Vision is effected through the absorption of a photon by the
chromophore, which is isomerised to the all-trans form, promoting a
conformational change in the protein.
Opsins are the photoreceptors of animal retinas [5]: vertebrate rhodopsin
is found in rod cells and mediates scotopic vision; red, green and blue
opsins are found in cone cells and mediate photopic vision. The opsins from
the lateral eyes and median ocelli of the horseshoe crab, Limulus polyphemus,
are likely to be the visible-wavelength rhodopsins in this animal [6]. In
the lateral eye, expression of the opsin gene is restricted to the photo-
receptor cells of the ommatidia [6]. Limulus opsins, and closely related
crayfish, mantid and honeybee rhodopsins, are most similar to the opsin
from the R1-6 photoreceptors of flies.
OPSINLTRLEYE is a 5-element fingerprint that provides a signature for
lateral eye and related opsins. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the lateral eye opsins but distinguish
them from the rest of the rhodopsin-like superfamily - motif 1 lies at the
N-terminus; motif 2 lies at the C-terminus of TM domain 2; motif 3 lies in
the third cytoplasmic loop; and motifs 4 and 5 lie at the C-terminus. A
single iteration on OWL28.1 was required to reach convergence, no further
sequences being identified beyond the starting set. Several partial matches
were also found, all of which are Drosophila RH1/RH2 opsins.
An update on SPTR37_9f identified a true set of 11 sequences, and 7
partial matches.
SUMMARY INFORMATION
11 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
7 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 11 11 11 11 11
4| 0 0 0 0 0
3| 0 0 0 0 0
2| 7 3 3 1 0
--+--------------------------
| 1 2 3 4 5
True positives..
O76124 OPSD_SPHSP OPSD_CAMAB OPS1_SCHGR
O76123 OPSD_CATBO O76125 OPSD_APIME
OPS1_LIMPO OPS2_LIMPO OPSD_PROCL
Subfamily: Codes involving 2 elements
Subfamily True positives..
OPS6_DROME OPS2_DROME OPS1_DROPS OPS1_DROME
OPS2_DROPS OPS1_CALVI OPS1_PATYE
PROTEIN TITLES
O76124 OPSIN - PAPILIO XUTHUS.
OPSD_SPHSP RHODOPSIN (OPSIN) - SPHODROMANTIS SP. (MANTIS).
OPSD_CAMAB RHODOPSIN - CAMPONOTUS ABDOMINALIS (CARPENTER ANT).
OPS1_SCHGR OPSIN 1 - SCHISTOCERCA GREGARIA (DESERT LOCUST).
O76123 OPSIN - PAPILIO XUTHUS.
OPSD_CATBO RHODOPSIN - CATAGLYPHIS BOMBYCINA (SAHARAN SILVER ANT).
O76125 OPSIN - PAPILIO XUTHUS.
OPSD_APIME RHODOPSIN, LONG-WAVELENGTH (OPSIN, GREEN-SENSITIVE) - APIS M
OPS1_LIMPO LATERAL EYE OPSIN - LIMULUS POLYPHEMUS (ATLANTIC HORSESHOE C
OPS2_LIMPO OCELLAR OPSIN - LIMULUS POLYPHEMUS (ATLANTIC HORSESHOE CRAB)
OPSD_PROCL RHODOPSIN - PROCAMBARUS CLARKII (RED SWAMP CRAYFISH).
OPS6_DROME OPSIN RH6 - DROSOPHILA MELANOGASTER (FRUIT FLY).
OPS2_DROME OPSIN RH2 (OCELLAR OPSIN) - DROSOPHILA MELANOGASTER (FRUIT F
OPS1_DROPS OPSIN RH1 (OUTER R1-R6 PHOTORECEPTOR CELLS OPSIN) - DROSOPHI
OPS1_DROME OPSIN RH1 (OUTER R1-R6 PHOTORECEPTOR CELLS OPSIN) - DROSOPHI
OPS2_DROPS OPSIN RH2 (OCELLAR OPSIN) - DROSOPHILA PSEUDOOBSCURA (FRUIT
OPS1_CALVI OPSIN RH1 (OUTER R1-R6 PHOTORECEPTOR CELLS OPSIN) - CALLIPHO
OPS1_PATYE RHODOPSIN, GQ-COUPLED - PATINOPECTEN YESSOENSIS (EZO GIANT S
SCAN HISTORY
OWL28_1 1 75 NSINGLE
SPTR37_9f 3 100 NSINGLE
INITIAL MOTIF SETS
OPSINLTRLEYE1 Length of motif = 15 Motif number = 1
Lateral eye opsin motif I - 1
PCODE ST INT
WYQFPPMNPLWHAIL CBU32501 43 43
WYQFPPMNPLWHGLL OPSD_SPHSP 41 41
WYQFPPMNPLWHALL CAU32502 43 43
WYAFPPMNPLWYSIL OPS1_LIMPO 36 36
WYAFPPMNPLWYSIL OPS2_LIMPO 36 36
WYQYPPLNPMWHGIL AMU26026 41 41
WYQYPPMNPMMYPLL OPSD_PROCL 42 42
OPSINLTRLEYE2 Length of motif = 13 Motif number = 2
Lateral eye opsin motif II - 1
PCODE ST INT
MMLSMSPAMVINC CBU32501 103 45
MMLSMSPPMVINC OPSD_SPHSP 101 45
MMLCMSPAMVINC CAU32502 103 45
MMAFMMPTMTSNC OPS1_LIMPO 96 45
MMAFMMPTMASNC OPS2_LIMPO 96 45
MMFCMSPPMVINC AMU26026 101 45
MMFTMFPPMMVTC OPSD_PROCL 102 45
OPSINLTRLEYE3 Length of motif = 12 Motif number = 3
Lateral eye opsin motif III - 1
PCODE ST INT
SAENQSTSAECK CBU32501 266 150
SAENANTSAECK OPSD_SPHSP 264 150
SAENQSTSAECK CAU32502 266 150
NADQQKQSAECR OPS1_LIMPO 260 151
NADQQKQSAECR OPS2_LIMPO 260 151
SSENQNTSAECK AMU26026 264 150
NEEAQKTSAECR OPSD_PROCL 264 149
OPSINLTRLEYE4 Length of motif = 12 Motif number = 4
Lateral eye opsin motif IV - 1
PCODE ST INT
AALFQRFPSLAC CBU32501 342 64
AALNKKFPSLAC OPSD_SPHSP 340 64
AALFQKFPSLAC CAU32502 342 64
AALYQRFPSLAC OPS1_LIMPO 337 65
AALYQRFPSLAC OPS2_LIMPO 337 65
AALFAKFPSLAC AMU26026 340 64
AAMEKKLPCLSC OPSD_PROCL 340 64
OPSINLTRLEYE5 Length of motif = 14 Motif number = 5
Lateral eye opsin motif V - 1
PCODE ST INT
STTTTVTEGTEKPA CBU32501 364 10
QASGATTVSDEKSA OPSD_SPHSP 361 9
STTTTVTEGNEKPA CAU32502 364 10
SEASATTTMEEKPK OPS1_LIMPO 359 10
SEASATMTMEEKPK OPS2_LIMPO 359 10
TSGTTTVTDNEKSN AMU26026 363 11
SASTTTSSAEEKAE OPSD_PROCL 361 9
FINAL MOTIF SETS
OPSINLTRLEYE1 Length of motif = 15 Motif number = 1
Lateral eye opsin motif I - 3
PCODE ST INT
WYQFPPMNPMWHGLL O76124 45 45
WYQFPPMNPLWHGLL OPSD_SPHSP 41 41
WYQFPPMNPLWHALL OPSD_CAMAB 43 43
WYQFPPMNPLWHGLL OPS1_SCHGR 43 43
WYQFPPMNPLWHGLL O76123 45 45
WYQFPPMNPLWHAIL OPSD_CATBO 43 43
WYQFPPMNPLWYGLL O76125 45 45
WYQYPPLNPMWHGIL OPSD_APIME 41 41
WYAFPPMNPLWYSIL OPS1_LIMPO 36 36
WYAFPPMNPLWYSIL OPS2_LIMPO 36 36
WYQYPPMNPMMYPLL OPSD_PROCL 42 42
OPSINLTRLEYE2 Length of motif = 13 Motif number = 2
Lateral eye opsin motif II - 3
PCODE ST INT
MMLCMAPPMLINC O76124 105 45
MMLSMSPPMVINC OPSD_SPHSP 101 45
MMLCMSPAMVINC OPSD_CAMAB 103 45
MMFTMSAPMGINC OPS1_SCHGR 103 45
MMTCMAPPLVVNS O76123 105 45
MMLSMSPAMVINC OPSD_CATBO 103 45
MMACMAPPLIINS O76125 105 45
MMFCMSPPMVINC OPSD_APIME 101 45
MMAFMMPTMTSNC OPS1_LIMPO 96 45
MMAFMMPTMASNC OPS2_LIMPO 96 45
MMFTMFPPMMVTC OPSD_PROCL 102 45
OPSINLTRLEYE3 Length of motif = 12 Motif number = 3
Lateral eye opsin motif III - 3
PCODE ST INT
SSEAANTSAECK O76124 268 150
SAENANTSAECK OPSD_SPHSP 264 150
SAENQSTSAECK OPSD_CAMAB 266 150
SAEASQTSAECK OPS1_SCHGR 266 150
SSEAANTSAECK O76123 268 150
SAENQSTSAECK OPSD_CATBO 266 150
SSDAANTSAECK O76125 268 150
SSENQNTSAECK OPSD_APIME 264 150
NADQQKQSAECR OPS1_LIMPO 260 151
NADQQKQSAECR OPS2_LIMPO 260 151
NEEAQKTSAECR OPSD_PROCL 264 149
OPSINLTRLEYE4 Length of motif = 12 Motif number = 4
Lateral eye opsin motif IV - 3
PCODE ST INT
AALYQKFPSLAC O76124 344 64
AALNKKFPSLAC OPSD_SPHSP 340 64
AALFQKFPSLAC OPSD_CAMAB 342 64
AALEKKFPSLAC OPS1_SCHGR 342 64
AALYQRFPSLAC O76123 344 64
AALFQRFPSLAC OPSD_CATBO 342 64
AALYQRFPSLAC O76125 344 64
AALFAKFPSLAC OPSD_APIME 340 64
AALYQRFPSLAC OPS1_LIMPO 337 65
AALYQRFPSLAC OPS2_LIMPO 337 65
AAMEKKLPCLSC OPSD_PROCL 340 64
OPSINLTRLEYE5 Length of motif = 14 Motif number = 5
Lateral eye opsin motif V - 3
PCODE ST INT
VASGATTACEEKPS O76124 365 9
QASGATTVSDEKSA OPSD_SPHSP 361 9
STTTTVTEGNEKPA OPSD_CAMAB 364 10
VASGATTVSDEKSE OPS1_SCHGR 363 9
QVSGKTAVCEEKPS O76123 365 9
STTTTVTEGTEKPA OPSD_CATBO 364 10
VASGNTAVCEEKAP O76125 365 9
TSGTTTVTDNEKSN OPSD_APIME 363 11
SEASATTTMEEKPK OPS1_LIMPO 359 10
SEASATMTMEEKPK OPS2_LIMPO 359 10
SASTTTSSAEEKAE OPSD_PROCL 361 9
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