WORKLIST ENTRIES (1):
OPSINBLUE View alignment View Structure Blue-sensitive opsin signature
Type of fingerprint: COMPOUND with 6 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00238 OPSIN; PR00575 OPSINREDGRN; PR00576 OPSINRH1RH2
PRINTS; PR00577 OPSINRH3RH4; PR00578 OPSINLTRLEYE; PR01244 PEROPSIN
PRINTS; PR00666 PINOPSIN; PR00579 RHODOPSIN; PR00239 RHODOPSNTAIL
PRINTS; PR00667 RPERETINALR
INTERPRO; IPR001521
Creation date 11-SEP-1996; UPDATE 07-JUN-1999
1. APPLEBURY, M.L. AND HARGRAVE, P.A.
Molecular biology of the visual pigments.
VISION RES. 26(12) 1881-1895 (1986).
2. FRYXELL, K.J. AND MEYEROWITZ, E.M.
The evolution of rhodopsins and neurotransmitter receptors.
J.MOL.EVOL. 33(4) 367-378 (1991).
3. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
4. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
5. WATSON, S. AND ARKINSTALL,
Opsins.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.214-222.
Opsins, the light-absorbing molecules that mediate vision [1,2], are
integral membrane proteins that belong to a superfamily of G protein-coupled
receptors (GPCRs). The activating ligands of the different superfamily
members vary widely in structure and character, yet the proteins appear
faithfully to have conserved a basic structural framework, believed to
consist of 7 transmembrane (TM) helices. Although the sequences of these
proteins are very diverse, reflecting to some extent this broad range of
activating ligands, nevertheless, motifs have been identified in the TM
regions that are characteristic of virtually the entire superfamily [3,4].
Amongst the exceptions are the olfactory receptors, which cluster together
in a subfamily, which lacks significant matches with domains 2, 4 and 6.
Interestingly, the opsins also seem to be emerging as increasingly atypical
of the superfamily, clustering most strongly, in phylogenetic analyses, with
the olfactory receptors [4].
The visual pigments comprise an apoprotein (opsin), covalently linked to
the chromophore 11-cis-retinal. The covalent link is in the form of a
protonated Schiff base between the retinal and a lysine residue located in
TM domain 7. Vision is effected through the absorption of a photon by the
chromophore, which is isomerised to the all-trans form, promoting a
conformational change in the protein.
Opsins are the photoreceptors of animal retinas [5]: vertebrate rhodopsin
is found in rod cells and mediates scotopic vision; red, green and blue
opsins are found in cone cells and mediate photopic vision. Blue-sensitive
opsin has an absorption maximum at 420nm. The ratio of blue cones to rods
is ~1:200 [5]. Deficiency in blue opsin results in tritanopia colour
blindness.
OPSINBLUE is a 6-element fingerprint that provides a signature for blue-
sensitive opsins. The fingerprint was derived from an initial alignment
of 8 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the blue-sensitive opsins but distinguish them from the
rest of the rhodopsin-like superfamily - motifs 1 and 2 lie at the
N-terminus; motif 3 spans the second external loop; motif 4 spans the
N-terminal half of TM domain 5; motif 5 spans the third external loop; and
motif 6 lies at the C-terminus. A single iteration on OWL28.1 was required
to reach convergence, no further sequences being identified beyond the
starting set. Several partial matches were also found, all of which are
closely related green-sensitive opsins or rhodopsins.
An update on SPTR37_9f identified a true set of 13 sequences, and 2
partial matches.
SUMMARY INFORMATION
13 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
6| 13 13 13 13 13 13
5| 0 0 0 0 0 0
4| 0 1 1 1 1 0
3| 0 0 0 0 0 0
2| 0 1 0 0 0 1
--+-------------------------------
| 1 2 3 4 5 6
True positives..
O46554 OPSB_SAIBB O57605 OPSB_HUMAN
O70363 OPSB_MOUSE OPSB_RAT OPSB_BOVIN
OPSV_CHICK OPSV_XENLA OPSB_CARAU OPSB_ASTFA
OPSB_CHICK
Subfamily: Codes involving 4 elements
Subfamily True positives..
OPSB_ORYLA
Subfamily: Codes involving 2 elements
Subfamily True positives..
OPSV_ORYLA
PROTEIN TITLES
O46554 BLUE OPSIN - CEBUS OLIVACEUS (WEEPER CAPUCHIN MONKEY).
OPSB_SAIBB BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - SAI
O57605 UV-SENSITIVE CONE OPSIN - MELOPSITTACUS UNDULATUS (BUDGERIGA
OPSB_HUMAN BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - HOM
O70363 BLUE CONE OPSIN PIGMENT - RATTUS NORVEGICUS (RAT).
OPSB_MOUSE BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - MUS
OPSB_RAT BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - RAT
OPSB_BOVIN BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - BOS
OPSV_CHICK VIOLET-SENSITIVE OPSIN (VIOLET CONE PHOTORECEPTOR PIGMENT) (
OPSV_XENLA VIOLET-SENSITIVE OPSIN (VIOLET CONE PHOTORECEPTOR PIGMENT) (
OPSB_CARAU BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - CAR
OPSB_ASTFA BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - AST
OPSB_CHICK BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) - GAL
OPSB_ORYLA BLUE-SENSITIVE OPSIN (BLUE CONE PHOTORECEPTOR PIGMENT) (KFH-
OPSV_ORYLA PUTATIVE VIOLET-SENSITIVE OPSIN (VIOLET CONE PHOTORECEPTOR P
SCAN HISTORY
OWL28_1 1 50 NSINGLE
SPTR37_9f 2 33 NSINGLE
INITIAL MOTIF SETS
OPSINBLUE1 Length of motif = 9 Motif number = 1
Blue opsin motif I - 1
PCODE ST INT
MSEEEFYLF OPSB_HUMAN 4 4
SSDDDFYLF OPSV_CHICK 2 2
EFHEDFYIP OPSB_CARAU 6 6
DLPEDFYIP OPSB_CHICK 9 9
SEEEEFLLF A54679 5 5
LEEEDFYLF XLU23463 2 2
SGEDDFYLF B54679 2 2
EFQEDFYIP S39028 7 7
OPSINBLUE2 Length of motif = 13 Motif number = 2
Blue opsin motif II - 1
PCODE ST INT
KNISSVGPWDGPQ OPSB_HUMAN 13 0
TNGSVPGPWDGPQ OPSV_CHICK 11 0
NNLSAYSPFLVPQ OPSB_CARAU 20 5
PNITALSPFLVPQ OPSB_CHICK 23 5
KNISLVGPWDGPQ A54679 14 0
KNVSNVSPFDGPQ XLU23463 11 0
QNISSVGPWDGPQ B54679 11 0
NNITALSPFLVPQ S39028 21 5
OPSINBLUE3 Length of motif = 11 Motif number = 3
Blue opsin motif III - 1
PCODE ST INT
TVGTKYRSESY OPSB_HUMAN 190 164
TVGTKYRSEYY OPSV_CHICK 188 164
TTNNKYNNESY OPSB_CARAU 197 164
TTDNKWHNESY OPSB_CHICK 200 164
TVGTKYYSEYY A54679 191 164
TVGTKYRSEYY XLU23463 188 164
TVGTKYRSEYY B54679 188 164
TTENKYNNESY S39028 198 164
OPSINBLUE4 Length of motif = 12 Motif number = 4
Blue opsin motif IV - 1
PCODE ST INT
YTWFLFIFCFIV OPSB_HUMAN 200 -1
YTWFLFIFCFIV OPSV_CHICK 198 -1
YVMFLFCFCFAV OPSB_CARAU 207 -1
YVLFLFTFCFGV OPSB_CHICK 210 -1
YTWFLFIFCYIV A54679 201 -1
YTWFIFIFCFVI XLU23463 198 -1
YTWFLFIFCFII B54679 198 -1
YVMFLFCFCFGF S39028 208 -1
OPSINBLUE5 Length of motif = 20 Motif number = 5
Blue opsin motif V - 1
PCODE ST INT
AFAMYMVNNRNHGLDLRLVT OPSB_HUMAN 267 55
ALAMYMVNNRDHGLDLRLVT OPSV_CHICK 265 55
SFSLWIVSHRGEEFDLRMAT OPSB_CARAU 274 55
AFALWVVTHRGRSFEVGLAS OPSB_CHICK 277 55
ALAMYIVNNRNHGVDLRLVT A54679 268 55
AMAMYMVTNRNHGLDLRLVT XLU23463 265 55
ALAMYMVNNRNHGLDLRLVT B54679 265 55
SFALWVVFNRGQSFDLRLGT S39028 275 55
OPSINBLUE6 Length of motif = 15 Motif number = 6
Blue opsin motif VI - 1
PCODE ST INT
IMKMVCGKAMTDESD OPSB_HUMAN 314 27
IMETVCGKPLTDDSD OPSV_CHICK 312 27
MMKMVCGKNIEEDEA OPSB_CARAU 321 27
LKLLFCGRSPFGDDE OPSB_CHICK 325 28
IMEMVCGKPMTDESE A54679 315 27
IMETVCGRPMSDDSS XLU23463 312 27
ILEMVCRKPMADESD B54679 312 27
MKLIFCGKSPFGDDE S39028 323 28
FINAL MOTIF SETS
OPSINBLUE1 Length of motif = 9 Motif number = 1
Blue opsin motif I - 2
PCODE ST INT
SEEEEFYLF O46554 5 5
PEEEEFYLF OPSB_SAIBB 5 5
SGEEEFYLF O57605 2 2
MSEEEFYLF OPSB_HUMAN 4 4
SGEDEFYLF O70363 2 2
SGEDDFYLF OPSB_MOUSE 2 2
SGEXEFYLF OPSB_RAT 2 2
SEEEEFLLF OPSB_BOVIN 5 5
SSDDDFYLF OPSV_CHICK 2 2
LEEEDFYLF OPSV_XENLA 2 2
EFHEDFYIP OPSB_CARAU 6 6
EFQEDFYIP OPSB_ASTFA 7 7
DLPEDFYIP OPSB_CHICK 9 9
OPSINBLUE2 Length of motif = 13 Motif number = 2
Blue opsin motif II - 2
PCODE ST INT
KNISSVGPWDGPQ O46554 14 0
KNISSVGPWDGPQ OPSB_SAIBB 14 0
KNGSIGGPWDGPQ O57605 11 0
KNISSVGPWDGPQ OPSB_HUMAN 13 0
QNISSVGPWDGPQ O70363 11 0
QNISSVGPWDGPQ OPSB_MOUSE 11 0
QNISSVGPWDGPQ OPSB_RAT 11 0
KNISLVGPWDGPQ OPSB_BOVIN 14 0
TNGSVPGPWDGPQ OPSV_CHICK 11 0
KNVSNVSPFDGPQ OPSV_XENLA 11 0
NNLSAYSPFLVPQ OPSB_CARAU 20 5
NNITALSPFLVPQ OPSB_ASTFA 21 5
PNITALSPFLVPQ OPSB_CHICK 23 5
OPSINBLUE3 Length of motif = 11 Motif number = 3
Blue opsin motif III - 2
PCODE ST INT
TVGTKYRSEYY O46554 191 164
TVGTKYRSEYY OPSB_SAIBB 191 164
TVGTKYRSEYY O57605 188 164
TVGTKYRSESY OPSB_HUMAN 190 164
TVGTKYRSEHY O70363 188 164
TVGTKYRSEYY OPSB_MOUSE 188 164
TVGTKYRSEHY OPSB_RAT 188 164
TVGTKYYSEYY OPSB_BOVIN 191 164
TVGTKYRSEYY OPSV_CHICK 188 164
TVGTKYRSEYY OPSV_XENLA 188 164
TTNNKYNNESY OPSB_CARAU 197 164
TTENKYNNESY OPSB_ASTFA 198 164
TTDNKWHNESY OPSB_CHICK 200 164
OPSINBLUE4 Length of motif = 12 Motif number = 4
Blue opsin motif IV - 2
PCODE ST INT
YTWFLFIFCFIV O46554 201 -1
YTWFLFIFCFIV OPSB_SAIBB 201 -1
YTWFLFIFCFIV O57605 198 -1
YTWFLFIFCFIV OPSB_HUMAN 200 -1
YTWFLFIFCFII O70363 198 -1
YTWFLFIFCFII OPSB_MOUSE 198 -1
YTWFLFIFCFII OPSB_RAT 198 -1
YTWFLFIFCYIV OPSB_BOVIN 201 -1
YTWFLFIFCFIV OPSV_CHICK 198 -1
YTWFIFIFCFVI OPSV_XENLA 198 -1
YVMFLFCFCFAV OPSB_CARAU 207 -1
YVMFLFCFCFGF OPSB_ASTFA 208 -1
YVLFLFTFCFGV OPSB_CHICK 210 -1
OPSINBLUE5 Length of motif = 20 Motif number = 5
Blue opsin motif V - 2
PCODE ST INT
ALAMYMVNNRNHGLDLRLVT O46554 268 55
ALAMYMVNNRNHGLDLRLVS OPSB_SAIBB 268 55
ALAMYMVNNREHGIDLRLVT O57605 265 55
AFAMYMVNNRNHGLDLRLVT OPSB_HUMAN 267 55
ALAMYMVNNRNHGLDLRLVT O70363 265 55
ALAMYMVNNRNHGLDLRLVT OPSB_MOUSE 265 55
ALAMYMVNNRNHGLYLRLVT OPSB_RAT 265 55
ALAMYIVNNRNHGVDLRLVT OPSB_BOVIN 268 55
ALAMYMVNNRDHGLDLRLVT OPSV_CHICK 265 55
AMAMYMVTNRNHGLDLRLVT OPSV_XENLA 265 55
SFSLWIVSHRGEEFDLRMAT OPSB_CARAU 274 55
SFALWVVFNRGQSFDLRLGT OPSB_ASTFA 275 55
AFALWVVTHRGRSFEVGLAS OPSB_CHICK 277 55
OPSINBLUE6 Length of motif = 15 Motif number = 6
Blue opsin motif VI - 2
PCODE ST INT
IMEMVCGKAMTDESD O46554 315 27
IMEMVCGKAMTDESD OPSB_SAIBB 315 27
IMEMVCGKPMTDDSD O57605 312 27
IMKMVCGKAMTDESD OPSB_HUMAN 314 27
ILEMVCRKPMTDESD O70363 312 27
ILEMVCRKPMADESD OPSB_MOUSE 312 27
ILEMVCRKPMTDESD OPSB_RAT 312 27
IMEMVCGKPMTDESE OPSB_BOVIN 315 27
IMETVCGKPLTDDSD OPSV_CHICK 312 27
IMETVCGRPMSDDSS OPSV_XENLA 312 27
MMKMVCGKNIEEDEA OPSB_CARAU 321 27
MKLIFCGKSPFGDDE OPSB_ASTFA 323 28
LKLLFCGRSPFGDDE OPSB_CHICK 325 28
User query: Display/Full Code "OPSINBLUE"