WORKLIST ENTRIES (1):
OGR1RECEPTOR View alignment View Structure OGR1 sphingosylphosphorylcholine receptor signature
Type of fingerprint: COMPOUND with 11 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01563 G2ARECEPTOR; PR01147 GPR4RECEPTOR
Creation date 17-AUG-2001
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. FUKUSHIMA, N., ISHII, I., CONTOS, J.J.A., WEINER, J.A. AND CHUN, J.
Lysophospholipid receptors.
ANNU.REV.PHARMACOL.TOXICOL. 41 507-534 (2001).
7. LYNCH, K.R. AND IM, D.-S.
Life on the edg.
TRENDS PHARMACOL.SCI. 20(12) 473-475 (1999).
8. XU, Y., ZHU, K., HONG, G., WU, W., BAUDHUIN, L.M., XIAO, Y. AND
DAMRON, D.S.
Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled
receptor 1.
NAT.CELL BIOL. 2(5) 261-267 (2000).
9. XU, Y. AND CASEY, G.
Identification of human OGR1, a novel G protein-coupled receptor that maps
to chromosome 14.
GENOMICS 35(2) 397-402 (1996).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine,
para-crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Lysophospholipids (LPs), such as lysophosphatidic acid (LPA), sphingosine
1-phosphate (S1P) and sphingosylphosphorylcholine (SPC), have long been
known to act as signalling molecules in addition to their roles as
intermediates in membrane biosynthesis [6]. They have roles in the
regulation of cell growth, differentiation, apoptosis and development, and
have been implicated in a wide range of pathophysiological conditions,
including: blood clotting, corneal wounding, subarachinoid haemorrhage,
inflammation and colitis [7]. A number of G protein-coupled receptors bind
members of the lysophospholipid family. These include: the cannabinoid
receptors; platelet activating factor receptor; OGR1, an SPC receptor
identified in ovarian cancer cell lines; PSP24, an orphan receptor that has
been proposed to bind LPA; and at least 8 closely related receptors, the EDG
family, that bind LPA and S1P [6].
SPC is produced from degradation of sphingomyelin and regulates many diverse
cellular functions, such as: proliferation, growth inhibition, smooth muscle
contraction and wound healing [6,8]. It is also thought to play a role in
the cardiovascular system and potently activates inwardly rectifying K+
channels, suggesting a possible role in regulation of heart rate [6].
The orphan receptor OGR1 has been identified as a high affinity receptor for
SPC [8]. OGR1 is expressed in ovarian cancer cell lines and also in spleen,
testis, small intestine, peripheral blood leukocytes, brain, heart, lung,
placenta and kidney. Expression has not been found in thymus, prostate,
ovary, colon, liver, skeletal muscle or pancreas [9]. Upon activation by
SPC, OGR1 couples to both Gi proteins, causing increases in intracellular
calcium, and Gq proteins, to activate MAP kinases, inhibiting proliferation.
SPC also causes regulation of ion channel activity, binding of activator
protein-1 to DNA, and expression of cell adhesion molecule-1 and
interleukin-6 [8].
OGR1RECEPTOR is an 11-element fingerprint that provides a signature for the
OGR1 sphingosylphosphorylcholine receptors. The fingerprint was derived from
an initial alignment of 2 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise the OGR1 receptors but distinguish them from the
rest of the lysophospholipid receptor family - motif 1 resides at the
N-terminus; motif 2 spans part of the N-terminus, leading into TM domain 1;
motif 3 lies in the first external loop, leading into TM domain 3; motif 4
spans the second cytoplasmic loop; motif 5 encodes the C-terminal portion of
TM domain 4, leading into the second external loop; motif 6 spans the third
cytoplasmic loop; motif 7 encodes the third external loop; motif 8 lies at
the C-terminus of TM domain 7; and motifs 9-11 reside at the C-terminus. A
single iteration on SPTR39.22_17.3f was required to reach convergence, no
further sequences being identified beyond the starting set.
SUMMARY INFORMATION
2 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
11| 2 2 2 2 2 2 2 2 2 2 2
10| 0 0 0 0 0 0 0 0 0 0 0
9| 0 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0 0 0
--+--------------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10 11
True positives..
GP68_HUMAN O46685
PROTEIN TITLES
GP68_HUMAN PROBABLE G PROTEIN-COUPLED RECEPTOR GPR68 (OVARIAN CANCER G
O46685 ORPHAN G PROTEIN-COUPLED RECEPTOR BRGR1B - Bos taurus (Bovin
SCAN HISTORY
SPTR39.22_17.3f 1 50 NSINGLE
INITIAL MOTIF SETS
OGR1RECEPTOR1 Length of motif = 16 Motif number = 1
OGR1 sphingosylphosphorylcholine receptor motif I - 1
PCODE ST INT
MGNITADNSSMSCTID GP68_HUMAN 1 1
MGNITADNTSMNCDID O46685 1 1
OGR1RECEPTOR2 Length of motif = 14 Motif number = 2
OGR1 sphingosylphosphorylcholine receptor motif II - 1
PCODE ST INT
DHTIHQTLAPVVYV GP68_HUMAN 16 -1
DHTIHQTLAPVVYV O46685 16 -1
OGR1RECEPTOR3 Length of motif = 13 Motif number = 3
OGR1 sphingosylphosphorylcholine receptor motif III - 1
PCODE ST INT
DLSCQVCGILLYE GP68_HUMAN 91 61
DLSCQVCGILLYE O46685 91 61
OGR1RECEPTOR4 Length of motif = 12 Motif number = 4
OGR1 sphingosylphosphorylcholine receptor motif IV - 1
PCODE ST INT
FRFHQFRTLKAA GP68_HUMAN 127 23
FRFHQFRTLKAA O46685 127 23
OGR1RECEPTOR5 Length of motif = 18 Motif number = 5
OGR1 sphingosylphosphorylcholine receptor motif V - 1
PCODE ST INT
KELLTSIYFLMHEEVIED GP68_HUMAN 148 9
KELLTSIYFLMHEEVVED O46685 148 9
OGR1RECEPTOR6 Length of motif = 14 Motif number = 6
OGR1 sphingosylphosphorylcholine receptor motif VI - 1
PCODE ST INT
RSHGTQKSRKDQIQ GP68_HUMAN 214 48
RSHGTQKSRKDQIQ O46685 214 48
OGR1RECEPTOR7 Length of motif = 14 Motif number = 7
OGR1 sphingosylphosphorylcholine receptor motif VII - 1
PCODE ST INT
SVWEASCDFAKGVF GP68_HUMAN 252 24
SLWESSCDFAKGIF O46685 252 24
OGR1RECEPTOR8 Length of motif = 10 Motif number = 8
OGR1 sphingosylphosphorylcholine receptor motif VIII - 1
PCODE ST INT
FVSETTHRDL GP68_HUMAN 288 22
FVSETTHRDL O46685 288 22
OGR1RECEPTOR9 Length of motif = 17 Motif number = 9
OGR1 sphingosylphosphorylcholine receptor motif IX - 1
PCODE ST INT
LRGACLAFLTCSRTGRA GP68_HUMAN 300 2
LRGACLAFLTCARTGRA O46685 300 2
OGR1RECEPTOR10 Length of motif = 15 Motif number = 10
OGR1 sphingosylphosphorylcholine receptor motif X - 1
PCODE ST INT
REAYPLGAPEASGKS GP68_HUMAN 317 0
REAYPLGAPEASGKS O46685 317 0
OGR1RECEPTOR11 Length of motif = 16 Motif number = 11
OGR1 sphingosylphosphorylcholine receptor motif XI - 1
PCODE ST INT
EEPELLTKLHPAFQTP GP68_HUMAN 336 4
EDPEVLTRLHPAFQTP O46685 332 0
FINAL MOTIF SETS
OGR1RECEPTOR1 Length of motif = 16 Motif number = 1
OGR1 sphingosylphosphorylcholine receptor motif I - 1
PCODE ST INT
MGNITADNSSMSCTID GP68_HUMAN 1 1
MGNITADNTSMNCDID O46685 1 1
OGR1RECEPTOR2 Length of motif = 14 Motif number = 2
OGR1 sphingosylphosphorylcholine receptor motif II - 1
PCODE ST INT
DHTIHQTLAPVVYV GP68_HUMAN 16 -1
DHTIHQTLAPVVYV O46685 16 -1
OGR1RECEPTOR3 Length of motif = 13 Motif number = 3
OGR1 sphingosylphosphorylcholine receptor motif III - 1
PCODE ST INT
DLSCQVCGILLYE GP68_HUMAN 91 61
DLSCQVCGILLYE O46685 91 61
OGR1RECEPTOR4 Length of motif = 12 Motif number = 4
OGR1 sphingosylphosphorylcholine receptor motif IV - 1
PCODE ST INT
FRFHQFRTLKAA GP68_HUMAN 127 23
FRFHQFRTLKAA O46685 127 23
OGR1RECEPTOR5 Length of motif = 18 Motif number = 5
OGR1 sphingosylphosphorylcholine receptor motif V - 1
PCODE ST INT
KELLTSIYFLMHEEVIED GP68_HUMAN 148 9
KELLTSIYFLMHEEVVED O46685 148 9
OGR1RECEPTOR6 Length of motif = 14 Motif number = 6
OGR1 sphingosylphosphorylcholine receptor motif VI - 1
PCODE ST INT
RSHGTQKSRKDQIQ GP68_HUMAN 214 48
RSHGTQKSRKDQIQ O46685 214 48
OGR1RECEPTOR7 Length of motif = 14 Motif number = 7
OGR1 sphingosylphosphorylcholine receptor motif VII - 1
PCODE ST INT
SVWEASCDFAKGVF GP68_HUMAN 252 24
SLWESSCDFAKGIF O46685 252 24
OGR1RECEPTOR8 Length of motif = 10 Motif number = 8
OGR1 sphingosylphosphorylcholine receptor motif VIII - 1
PCODE ST INT
FVSETTHRDL GP68_HUMAN 288 22
FVSETTHRDL O46685 288 22
OGR1RECEPTOR9 Length of motif = 17 Motif number = 9
OGR1 sphingosylphosphorylcholine receptor motif IX - 1
PCODE ST INT
LRGACLAFLTCSRTGRA GP68_HUMAN 300 2
LRGACLAFLTCARTGRA O46685 300 2
OGR1RECEPTOR10 Length of motif = 15 Motif number = 10
OGR1 sphingosylphosphorylcholine receptor motif X - 1
PCODE ST INT
REAYPLGAPEASGKS GP68_HUMAN 317 0
REAYPLGAPEASGKS O46685 317 0
OGR1RECEPTOR11 Length of motif = 16 Motif number = 11
OGR1 sphingosylphosphorylcholine receptor motif XI - 1
PCODE ST INT
EEPELLTKLHPAFQTP GP68_HUMAN 336 4
EDPEVLTRLHPAFQTP O46685 332 0
User query: Display/Full Code "OGR1RECEPTOR"