WORKLIST ENTRIES (1):
NRPEPTIDEY4R View alignment View Structure Neuropeptide Y4 receptor signature
Type of fingerprint: COMPOUND with 10 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01012 NRPEPTIDEYR; PR01013 NRPEPTIDEY1R; PR01014 NRPEPTIDEY2R
PRINTS; PR01016 NRPEPTIDEY5R; PR01017 NRPEPTIDEY6R
INTERPRO; IPR001933
Creation date 30-NOV-1998; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Neuropeptide Y.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.194-198.
7. BARD, J.A., WALKER, M.W., BRANCHEK, T.A. AND WEINSHANK, R.L.
Cloning and functional expression of a human Y4 subtype receptor for
pancreatic polypeptide, neuropeptide Y, and peptide YY.
J.BIOL.CHEM. 270 26762-26765 (1995).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Neuropeptide Y (NPY) is one of the most abundant peptides in mammalian
brain, inducing a variety of behavioural effects (e.g., stimulation of food
intake, anxiety, facilitation of learning and memory, and regulation of the
cardiovascular and neuroendocrine systems) [6]. In the periphery, NPY
stimulates vascular smooth muscle contraction and modulates hormone
secretion. NPY has been implicated in the pathophysiology of hypertension,
congestive heart failure, affective disorders and appetite regulation [6].
Several pharmacologically distinct neuropeptide Y receptors have been
characterised, designated NPY Y1-Y6. Y4 receptors show highest similarity
to Y1 receptors from human, rat and mouse [7]. It is believed that they
may have potential roles in central nervous system, cardiovascular and
gastrointestinal function [7].
NRPEPTIDEY4R is a 10-element fingerprint that provides a signature for
neuropeptide Y4 receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the Y4 receptors but distinguish them from the rest of
the neuropeptide Y family - motifs 1-3 span the N-terminus, leading into
TM domain 1; motifs 4 and 5 span the C-terminus of TM domain 4, leading
into second external loop; motif 6 lies in the third cytoplasmic loop; motif
7 lies in the third external loop; and motifs 8-10 span the C-terminus. A
single iteration on OWL30.2 was required to reach convergence, no further
sequences being identified beyond the starting set. A single partial match
was found, AF030245, a zebrafish neuropeptide Y receptor that matches motifs
7 and 9.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
10| 3 3 3 3 3 3 3 3 3 3
9| 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0 0
--+---------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10
True positives..
NY4R_MOUSE NY4R_RAT NY4R_HUMAN
PROTEIN TITLES
NY4R_MOUSE NEUROPEPTIDE Y RECEPTOR TYPE 4 (NPY4-R) (PANCREATIC POLYPEPT
NY4R_RAT NEUROPEPTIDE Y RECEPTOR TYPE 4 (NPY4-R) (PANCREATIC POLYPEPT
NY4R_HUMAN NEUROPEPTIDE Y RECEPTOR TYPE 4 (NPY4-R) (PANCREATIC POLYPEPT
SCAN HISTORY
OWL30_2 1 50 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
NRPEPTIDEY4R1 Length of motif = 16 Motif number = 1
Neuropeptide Y4 receptor motif I - 1
PCODE ST INT
NTSHLLALLLPKSPQG NY4R_HUMAN 2 2
NTSHFLAPLFPGSLQG NY4R_MOUSE 2 2
NTSHLMASLSPAFLQG NY4R_RAT 2 2
NRPEPTIDEY4R2 Length of motif = 14 Motif number = 2
Neuropeptide Y4 receptor motif II - 1
PCODE ST INT
NRSKPLGTPYNFSE NY4R_HUMAN 19 1
NGTNPLDSPYNFSD NY4R_MOUSE 19 1
NGTNPLDSLYNLSD NY4R_RAT 19 1
NRPEPTIDEY4R3 Length of motif = 14 Motif number = 3
Neuropeptide Y4 receptor motif III - 1
PCODE ST INT
CQDSVDVMVFIVTS NY4R_HUMAN 34 1
CQDSAELLAFIITT NY4R_MOUSE 34 1
CQDSADLLAFIITT NY4R_RAT 34 1
NRPEPTIDEY4R4 Length of motif = 16 Motif number = 4
Neuropeptide Y4 receptor motif IV - 1
PCODE ST INT
LANSILENVFHKNHSK NY4R_HUMAN 175 127
LANSTLNDLFHYNHSK NY4R_MOUSE 175 127
LANSILNDLFHYNHSK NY4R_RAT 175 127
NRPEPTIDEY4R5 Length of motif = 11 Motif number = 5
Neuropeptide Y4 receptor motif V - 1
PCODE ST INT
KALEFLADKVV NY4R_HUMAN 190 -1
KVVEFLEDKVV NY4R_MOUSE 190 -1
KVVEFLEDKVV NY4R_RAT 190 -1
NRPEPTIDEY4R6 Length of motif = 14 Motif number = 6
Neuropeptide Y4 receptor motif VI - 1
PCODE ST INT
FHKGTYSLRAGHMK NY4R_HUMAN 248 47
FHAHACSSRAGQMK NY4R_MOUSE 248 47
FHTHTCSSRVGQMK NY4R_RAT 248 47
NRPEPTIDEY4R7 Length of motif = 12 Motif number = 7
Neuropeptide Y4 receptor motif VII - 1
PCODE ST INT
WHHEAIPICHGN NY4R_HUMAN 290 28
WYQEAIPACHGN NY4R_MOUSE 290 28
WYQEAIPACHGN NY4R_RAT 290 28
NRPEPTIDEY4R8 Length of motif = 12 Motif number = 8
Neuropeptide Y4 receptor motif VIII - 1
PCODE ST INT
FKKEIKALVLTC NY4R_HUMAN 329 27
FKKDIKALVLTC NY4R_MOUSE 329 27
FKKDIKALVLTC NY4R_RAT 329 27
NRPEPTIDEY4R9 Length of motif = 14 Motif number = 9
Neuropeptide Y4 receptor motif IX - 1
PCODE ST INT
PLEESEHLPLSTVH NY4R_HUMAN 345 4
PQGESEHLPLSTVH NY4R_MOUSE 345 4
PQGEPEPLPLSTVH NY4R_RAT 345 4
NRPEPTIDEY4R10 Length of motif = 15 Motif number = 10
Neuropeptide Y4 receptor motif X - 1
PCODE ST INT
TEVSKGSLRLSGRSN NY4R_HUMAN 359 0
TDLSKGSMRMGSKSN NY4R_MOUSE 359 0
TDLSKGSMRMGSKSN NY4R_RAT 359 0
FINAL MOTIF SETS
NRPEPTIDEY4R1 Length of motif = 16 Motif number = 1
Neuropeptide Y4 receptor motif I - 2
PCODE ST INT
NTSHFLAPLFPGSLQG NY4R_MOUSE 2 2
NTSHLMASLSPAFLQG NY4R_RAT 2 2
NTSHLLALLLPKSPQG NY4R_HUMAN 2 2
NRPEPTIDEY4R2 Length of motif = 14 Motif number = 2
Neuropeptide Y4 receptor motif II - 2
PCODE ST INT
NGTNPLDSPYNFSD NY4R_MOUSE 19 1
NGTNPLDSLYNLSD NY4R_RAT 19 1
NRSKPLGTPYNFSE NY4R_HUMAN 19 1
NRPEPTIDEY4R3 Length of motif = 14 Motif number = 3
Neuropeptide Y4 receptor motif III - 2
PCODE ST INT
CQDSAELLAFIITT NY4R_MOUSE 34 1
CQDSADLLAFIITT NY4R_RAT 34 1
CQDSVDVMVFIVTS NY4R_HUMAN 34 1
NRPEPTIDEY4R4 Length of motif = 16 Motif number = 4
Neuropeptide Y4 receptor motif IV - 2
PCODE ST INT
LANSTLNDLFHYNHSK NY4R_MOUSE 175 127
LANSILNDLFHYNHSK NY4R_RAT 175 127
LANSILENVFHKNHSK NY4R_HUMAN 175 127
NRPEPTIDEY4R5 Length of motif = 11 Motif number = 5
Neuropeptide Y4 receptor motif V - 2
PCODE ST INT
KVVEFLEDKVV NY4R_MOUSE 190 -1
KVVEFLEDKVV NY4R_RAT 190 -1
KALEFLADKVV NY4R_HUMAN 190 -1
NRPEPTIDEY4R6 Length of motif = 14 Motif number = 6
Neuropeptide Y4 receptor motif VI - 2
PCODE ST INT
FHAHACSSRAGQMK NY4R_MOUSE 248 47
FHTHTCSSRVGQMK NY4R_RAT 248 47
FHKGTYSLRAGHMK NY4R_HUMAN 248 47
NRPEPTIDEY4R7 Length of motif = 12 Motif number = 7
Neuropeptide Y4 receptor motif VII - 2
PCODE ST INT
WYQEAIPACHGN NY4R_MOUSE 290 28
WYQEAIPACHGN NY4R_RAT 290 28
WHHEAIPICHGN NY4R_HUMAN 290 28
NRPEPTIDEY4R8 Length of motif = 12 Motif number = 8
Neuropeptide Y4 receptor motif VIII - 2
PCODE ST INT
FKKDIKALVLTC NY4R_MOUSE 329 27
FKKDIKALVLTC NY4R_RAT 329 27
FKKEIKALVLTC NY4R_HUMAN 329 27
NRPEPTIDEY4R9 Length of motif = 14 Motif number = 9
Neuropeptide Y4 receptor motif IX - 2
PCODE ST INT
PQGESEHLPLSTVH NY4R_MOUSE 345 4
PQGEPEPLPLSTVH NY4R_RAT 345 4
PLEESEHLPLSTVH NY4R_HUMAN 345 4
NRPEPTIDEY4R10 Length of motif = 15 Motif number = 10
Neuropeptide Y4 receptor motif X - 2
PCODE ST INT
TDLSKGSMRMGSKSN NY4R_MOUSE 359 0
TDLSKGSMRMGSKSN NY4R_RAT 359 0
TEVSKGSLRLSGRSN NY4R_HUMAN 359 0
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