WORKLIST ENTRIES (1):
NPFFRECEPTOR View alignment View Structure Neuropeptide FF receptor family signature
Type of fingerprint: COMPOUND with 6 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01571 NPFFRECEPTR1; PR01572 NPFFRECEPTR2
Creation date 13-SEP-2001
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. PANULA, P., AARNISALO, A.A. AND WASOWICZ, K.
Neuropeptide FF, a mammalian neuropeptide with multiple functions.
PROG.NEUROBIOL. 48(4-5) 461-487 (1996).
7. KOTANI, M., MOLLEREAU, C., DETHEUX, M., LE POUL, E., BREZILLON, S.,
VAKILI, J., MAZARGUIL, H., VASSART, G., ZAJAC, J.M. AND PARMENTIER, M.
Functional characterization of a human receptor for neuropeptide FF and
related peptides.
BR.J.PHARMACOL. 133 138-144 (2001).
8. BONINI, J.A., JONES, K.A., ADHAM, N., FORRAY, C., ARTYMYSHYN, R., DURKIN,
M.M., SMITH, K.E., TAMM, J.A., BOTEJU, L.W., LAKHLANI, P.P., RADDATZ, R.,
YAO, W.J., OGOZALEK, K.L., BOYLE, N., KOURANOVA, E.V., QUAN, Y., VAYSSE,
P.J., WETZEL, J.M., BRANCHEK, T.A., GERALD, C. AND BOROWSKY, B.
Identification and characterization of two G protein-coupled receptors for
neuropeptide FF.
J.BIOL.CHEM. 275(50) 39324-39331 (2000).
9. HINUMA, S., SHINTANI, Y., FUKUSUMI, S., IIJIMA, N., MATSUMOTO, Y.,
HOSOYA, M., FUJII, R., WATANABE, T., KIKUCHI, K., TERAO, Y., YANO, T.,
YAMAMOTO, T., KAWAMATA, Y., HABATA, Y., ASADA, M., KITADA, C., KUROKAWA, T.,
ONDA, H., NISHIMURA, O., TANAKA, M., IBATA, Y. AND FUJINO, M.
New neuropeptides containing carboxy-terminal RFamide and their receptor in
mammals.
NAT.CELL BIOL. 2(10) 703-708 (2000).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine,
para-crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Neuropeptide FF (and neuropeptide AF, which is also derived from the same
precursor) belong to a family of neuropeptides containing an RF-amide motif
at their C-terminus [6]. Neuropeptide FF (NPFF) is found at high
concentrations in the posterior pituitary, spinal cord, hypothalamus and
medulla and is believed to be involved in pain modulation, opioid tolerance,
cardiovascular regulation, memory and neuroendocrine regulation [6,7]. Two G
protein-coupled receptors for NPFF have been identified; both are expressed
in the central nervous system and couple to Gi proteins to inhibit adenylyl
cyclase [7-9].
NPFFRECEPTOR is a 6-element fingerprint that provides a signature for the
neuropeptide FF receptor family. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the neuropeptide FF receptors but distinguish them from
the rest of the rhodopsin-like superfamily - motif 1 resides at the
N-terminus; motif 2 lies in TM domain 1; motif 3 is located at the
C-terminus of TM domain 2, leading into the first external loop; motif 4
spans the C-terminal portion of TM domain 3 and the second cytoplasmic loop;
motif 5 lies in the third external loop, leading into TM domain 7; and
motif 6 resides at the C-terminus of TM domain 7. A single iteration on
SPTR39.22_17.3f was required to reach convergence, no further sequences
being identified beyond the starting set.
SUMMARY INFORMATION
4 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
6| 4 4 4 4 4 4
5| 0 0 0 0 0 0
4| 0 0 0 0 0 0
3| 0 0 0 0 0 0
2| 0 0 0 0 0 0
--+-------------------------------
| 1 2 3 4 5 6
True positives..
NFF1_HUMAN NFF1_RAT NFF2_RAT NFF2_HUMAN
PROTEIN TITLES
NFF1_HUMAN NEUROPEPTIDE FF RECEPTOR 1 (RFAMIDE-RELATED PEPTIDE RECEPTOR
NFF1_RAT NEUROPEPTIDE FF RECEPTOR 1 (RFAMIDE-RELATED PEPTIDE RECEPTOR
NFF2_RAT NEUROPEPTIDE FF RECEPTOR 2 (NEUROPEPTIDE G PROTEIN-COUPLED R
NFF2_HUMAN NEUROPEPTIDE FF RECEPTOR 2 (NEUROPEPTIDE G PROTEIN-COUPLED R
SCAN HISTORY
SPTR39.22_17.3f 1 80 NSINGLE
INITIAL MOTIF SETS
NPFFRECEPTOR1 Length of motif = 14 Motif number = 1
Neuropeptide FF receptor family motif I - 1
PCODE ST INT
NLTFSSYYQHTSPV NFF1_HUMAN 29 29
SLTFSSYYQHSSPV NFF1_RAT 29 29
NITYVNYYLHQPQV NFF2_HUMAN 133 133
NITYMNYYLHQPHV NFF2_RAT 31 31
NPFFRECEPTOR2 Length of motif = 13 Motif number = 2
Neuropeptide FF receptor family motif II - 1
PCODE ST INT
FLLCMVGNTLVCF NFF1_HUMAN 54 11
FLLCMVGNTLVCF NFF1_RAT 54 11
FFLCMMGNTVVCF NFF2_HUMAN 158 11
FFLCMVGNTVVCF NFF2_RAT 56 11
NPFFRECEPTOR3 Length of motif = 18 Motif number = 3
Neuropeptide FF receptor family motif III - 1
PCODE ST INT
GIFCMPTTLVDNLITGWP NFF1_HUMAN 93 26
GIFCMPTTLVDNLITGWP NFF1_RAT 93 26
GIFCMPITLLDNIIAGWP NFF2_HUMAN 197 26
GIFCMPITLLDNIIAGWP NFF2_RAT 95 26
NPFFRECEPTOR4 Length of motif = 15 Motif number = 4
Neuropeptide FF receptor family motif IV - 1
PCODE ST INT
FRCIVHPFREKLTLR NFF1_HUMAN 142 31
FRCIVHPFREKLTLR NFF1_RAT 142 31
FQCVVYPFKPKLTIK NFF2_HUMAN 246 31
FRCVVYPFKPKLTVK NFF2_RAT 144 31
NPFFRECEPTOR5 Length of motif = 13 Motif number = 5
Neuropeptide FF receptor family motif V - 1
PCODE ST INT
TVYAFPFAHWLAF NFF1_HUMAN 307 150
SVYAFPLAHWLAF NFF1_RAT 309 152
NIYIYPFAHWLAF NFF2_HUMAN 413 152
NIYVYPFAHWLAF NFF2_RAT 310 151
NPFFRECEPTOR6 Length of motif = 11 Motif number = 6
Neuropeptide FF receptor family motif VI - 1
PCODE ST INT
ENFRRGFQAAF NFF1_HUMAN 334 14
ENFRRGFQAAF NFF1_RAT 336 14
ENFRRGFQEAF NFF2_HUMAN 440 14
ENFRSGFQDAF NFF2_RAT 337 14
FINAL MOTIF SETS
NPFFRECEPTOR1 Length of motif = 14 Motif number = 1
Neuropeptide FF receptor family motif I - 1
PCODE ST INT
NLTFSSYYQHTSPV NFF1_HUMAN 29 29
SLTFSSYYQHSSPV NFF1_RAT 29 29
NITYMNYYLHQPHV NFF2_RAT 31 31
NITYVNYYLHQPQV NFF2_HUMAN 133 133
NPFFRECEPTOR2 Length of motif = 13 Motif number = 2
Neuropeptide FF receptor family motif II - 1
PCODE ST INT
FLLCMVGNTLVCF NFF1_HUMAN 54 11
FLLCMVGNTLVCF NFF1_RAT 54 11
FFLCMVGNTVVCF NFF2_RAT 56 11
FFLCMMGNTVVCF NFF2_HUMAN 158 11
NPFFRECEPTOR3 Length of motif = 18 Motif number = 3
Neuropeptide FF receptor family motif III - 1
PCODE ST INT
GIFCMPTTLVDNLITGWP NFF1_HUMAN 93 26
GIFCMPTTLVDNLITGWP NFF1_RAT 93 26
GIFCMPITLLDNIIAGWP NFF2_RAT 95 26
GIFCMPITLLDNIIAGWP NFF2_HUMAN 197 26
NPFFRECEPTOR4 Length of motif = 15 Motif number = 4
Neuropeptide FF receptor family motif IV - 1
PCODE ST INT
FRCIVHPFREKLTLR NFF1_HUMAN 142 31
FRCIVHPFREKLTLR NFF1_RAT 142 31
FRCVVYPFKPKLTVK NFF2_RAT 144 31
FQCVVYPFKPKLTIK NFF2_HUMAN 246 31
NPFFRECEPTOR5 Length of motif = 13 Motif number = 5
Neuropeptide FF receptor family motif V - 1
PCODE ST INT
TVYAFPFAHWLAF NFF1_HUMAN 307 150
SVYAFPLAHWLAF NFF1_RAT 309 152
NIYVYPFAHWLAF NFF2_RAT 310 151
NIYIYPFAHWLAF NFF2_HUMAN 413 152
NPFFRECEPTOR6 Length of motif = 11 Motif number = 6
Neuropeptide FF receptor family motif VI - 1
PCODE ST INT
ENFRRGFQAAF NFF1_HUMAN 334 14
ENFRRGFQAAF NFF1_RAT 336 14
ENFRSGFQDAF NFF2_RAT 337 14
ENFRRGFQEAF NFF2_HUMAN 440 14
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