WORKLIST ENTRIES (1):
NEUROKININ3R View alignment View Structure Neurokinin NK3 receptor signature
Type of fingerprint: COMPOUND with 9 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00244 NEUROKININR; PR01024 NEUROKININ1R; PR01025 NEUROKININ2R
INTERPRO; IPR001013
Creation date 29-NOV-1998; UPDATE 03-JUL-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. YOKOTA, Y., SASAI, Y., TANAKA, K., FUJIWARA, T, TSUCHIDA, K.,
SHIGEMOTO, R., KAKIZUKA, A., OHKUBO, H. AND NAKANISHI, S.
Molecular characterisation of a functional cDNA for rat substance P
receptor.
J.BIOL.CHEM. 264(30) 17649-17652 (1989).
7. WATSON, S. AND ARKINSTALL, S.
Tachykinins.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.261-270.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Neuropeptide receptors are present in very small quantities in the cell
and are embedded tightly in the plasma membrane. The neuropeptides exhibit
a high degree of functional diversity through both regulation of peptide
production and through peptide-receptor interaction [6]. The mammalian
tachykinin system consists of 3 distinct peptides: substance P, substance
K and neuromedin K. All possess a common spectrum of biological activities,
including sensory transmission in the nervous system and contraction/
relaxation of peripheral smooth muscles, and each interacts with a
specific receptor type.
NK3 receptors are distributed widely throughout the rat CNS, and are found
in high levels in cerebral cortex, basal ganglia and dorsal horn of the
spinal chord. They have limited distribution in peripheral tissues, and
are found in ganglia (e.g., myenteric plexus), kidney, and in a limited
number of smooth muscles (e.g., rat portal vein) [7]. NK3 receptors
activate the phosphoinositide pathway through a pertussis-toxin-insensitive
G protein, probably of the Gq/G11 class [7].
NEUROKININ3R is a 9-element fingerprint that provides a signature for the
neurokinin NK3 receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the NK3 receptors but distinguish them from the rest of
the neurokinin family - motifs 1-3 reside within the N-terminus; motifs 4
and 5 span the second external loop, leading into TM domain 5; motif 6 lies
in the third cytoplasmic loop; motif 7 spans the C-terminus of TM domain 6
and the third external loop; and motifs 8 and 9 reside at the C-terminus.
Two iterations on OWL30.2 were required to reach convergence, at which point
at true set comprising 5 sequences was identified. Several partial matches
were also found, all of which are homologues or fragments that fail to match
three or more motifs.
An update on SPTR37_9f identified a true set of 2 sequences, and 1
partial match.
SUMMARY INFORMATION
2 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
9| 2 2 2 2 2 2 2 2 2
8| 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0
6| 0 0 0 1 1 1 1 1 1
5| 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0
--+----------------------------------------------
| 1 2 3 4 5 6 7 8 9
True positives..
NK3R_HUMAN NK3R_RAT
Subfamily: Codes involving 6 elements
Subfamily True positives..
NK4R_HUMAN
PROTEIN TITLES
NK3R_HUMAN NEUROMEDIN K RECEPTOR (NKR) (NEUROKININ B RECEPTOR) (NK-3 RE
NK3R_RAT NEUROMEDIN K RECEPTOR (NKR) (NEUROKININ B RECEPTOR) (NK-3 RE
NK4R_HUMAN NEUROMEDIN K RECEPTOR (NKR) (NEUROKININ B RECEPTOR) (NK-4 RE
SCAN HISTORY
OWL30_2 2 100 NSINGLE
SPTR37_9f 2 35 NSINGLE
INITIAL MOTIF SETS
NEUROKININ3R1 Length of motif = 13 Motif number = 1
Neurokinin NK3 receptor motif I - 1
PCODE ST INT
ASVPTGENWTDGT NK3R_MOUSE 2 2
ASVPRGENWTDGT NK3R_RAT 2 2
ATLPAAETWIDGG NK3R_HUMAN 2 2
NEUROKININ3R2 Length of motif = 13 Motif number = 2
Neurokinin NK3 receptor motif II - 1
PCODE ST INT
GVGSHTGNLSAAL NK3R_MOUSE 16 1
EVGTHTGNLSSAL NK3R_RAT 16 1
GVGADAVNLTASL NK3R_HUMAN 16 1
NEUROKININ3R3 Length of motif = 20 Motif number = 3
Neurokinin NK3 receptor motif III - 1
PCODE ST INT
SALGLPVTSQAPSQVRDNLT NK3R_MOUSE 43 14
SALGLPATTQAPSQVRANLT NK3R_RAT 43 14
SALGLPVASPAPSQPWANLT NK3R_HUMAN 56 27
NEUROKININ3R4 Length of motif = 14 Motif number = 4
Neurokinin NK3 receptor motif IV - 1
PCODE ST INT
CLYSKIKVMPGRTL NK3R_MOUSE 206 143
CLYSKIKVMPGRTL NK3R_RAT 206 143
CLYSKTKVMPGRTL NK3R_HUMAN 219 143
NEUROKININ3R5 Length of motif = 17 Motif number = 5
Neurokinin NK3 receptor motif V - 1
PCODE ST INT
YVQWPEGPKQHFTYHII NK3R_MOUSE 221 1
YVQWPEGPKQHFTYHII NK3R_RAT 221 1
FVQWPEGPKQHFTYHII NK3R_HUMAN 234 1
NEUROKININ3R6 Length of motif = 10 Motif number = 6
Neurokinin NK3 receptor motif VI - 1
PCODE ST INT
GGEILGDTCD NK3R_MOUSE 263 25
GGEIPGDTCD NK3R_RAT 263 25
GGEIPGDTCD NK3R_HUMAN 276 25
NEUROKININ3R7 Length of motif = 14 Motif number = 7
Neurokinin NK3 receptor motif VII - 1
PCODE ST INT
LTAIYQQLNRWKYI NK3R_MOUSE 308 35
LTAIYQQLNRWKYI NK3R_RAT 308 35
LTAIYQQLNRWKYI NK3R_HUMAN 321 35
NEUROKININ3R8 Length of motif = 11 Motif number = 8
Neurokinin NK3 receptor motif VIII - 1
PCODE ST INT
FIQVSSYDELE NK3R_MOUSE 363 41
FIQVSSYDELE NK3R_RAT 363 41
FIKVSSYDELE NK3R_HUMAN 376 41
NEUROKININ3R9 Length of motif = 12 Motif number = 9
Neurokinin NK3 receptor motif IX - 1
PCODE ST INT
ELKTTRFHPTRQ NK3R_MOUSE 373 -1
ELKTTRFHPTRQ NK3R_RAT 373 -1
ELKTTRFHPNRQ NK3R_HUMAN 386 -1
FINAL MOTIF SETS
NEUROKININ3R1 Length of motif = 13 Motif number = 1
Neurokinin NK3 receptor motif I - 2
PCODE ST INT
ATLPAAETWIDGG NK3R_HUMAN 2 2
ASVPRGENWTDGT NK3R_RAT 2 2
NEUROKININ3R2 Length of motif = 13 Motif number = 2
Neurokinin NK3 receptor motif II - 2
PCODE ST INT
GVGADAVNLTASL NK3R_HUMAN 16 1
EVGTHTGNLSSAL NK3R_RAT 16 1
NEUROKININ3R3 Length of motif = 20 Motif number = 3
Neurokinin NK3 receptor motif III - 2
PCODE ST INT
SALGLPVASPAPSQPWANLT NK3R_HUMAN 56 27
SALGLPATTQAPSQVRANLT NK3R_RAT 43 14
NEUROKININ3R4 Length of motif = 14 Motif number = 4
Neurokinin NK3 receptor motif IV - 2
PCODE ST INT
CLYSKTKVMPGRTL NK3R_HUMAN 219 143
CLYSKIKVMPGRTL NK3R_RAT 206 143
NEUROKININ3R5 Length of motif = 17 Motif number = 5
Neurokinin NK3 receptor motif V - 2
PCODE ST INT
FVQWPEGPKQHFTYHII NK3R_HUMAN 234 1
YVQWPEGPKQHFTYHII NK3R_RAT 221 1
NEUROKININ3R6 Length of motif = 10 Motif number = 6
Neurokinin NK3 receptor motif VI - 2
PCODE ST INT
GGEIPGDTCD NK3R_HUMAN 276 25
GGEIPGDTCD NK3R_RAT 263 25
NEUROKININ3R7 Length of motif = 14 Motif number = 7
Neurokinin NK3 receptor motif VII - 2
PCODE ST INT
LTAIYQQLNRWKYI NK3R_HUMAN 321 35
LTAIYQQLNRWKYI NK3R_RAT 308 35
NEUROKININ3R8 Length of motif = 11 Motif number = 8
Neurokinin NK3 receptor motif VIII - 2
PCODE ST INT
FIKVSSYDELE NK3R_HUMAN 376 41
FIQVSSYDELE NK3R_RAT 363 41
NEUROKININ3R9 Length of motif = 12 Motif number = 9
Neurokinin NK3 receptor motif IX - 2
PCODE ST INT
ELKTTRFHPNRQ NK3R_HUMAN 386 -1
ELKTTRFHPTRQ NK3R_RAT 373 -1
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