WORKLIST ENTRIES (1):
MUSCRINICM5R View alignment View Structure Muscarinic M5 receptor signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00243 MUSCARINICR; PR00538 MUSCRINICM1R; PR00539 MUSCRINICM2R
PRINTS; PR00540 MUSCRINICM3R; PR00541 MUSCRINICM4R
INTERPRO; IPR000502
Creation date 01-JUN-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. KERLAVAGE, A.R., FRASER, C.M., CHUNG, F-Z. AND VENTER, J.C.
Molecular structure and evolution of adrenergic and cholinergic receptors.
PROTEINS 1 287-301 (1986).
7. WATSON, S. AND ARKINSTALL, S.
Acetylcholine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.7-18.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
The muscarinic acetylcholine receptors, present in the central nervous
system, spinal cord motoneurons and autonomic preganglia, modulate a
variety of physiological functions, including airway, eye and intestinal
smooth muscle contractions; heart rate; and glandular secretions. The
receptors mediate adenylate cyclase attenuation, calcium and potassium
channel activation, and phosphatidyl inositol turnover [6]. This diversity
may result from the occurrence of multiple receptor subtypes (of which 5
are currently known, designated M1 to M5), which have been classified
based on observed differences in ligand binding to receptors in membranes
from several tissues.
M5 mRNA has been found in the CNS, but a translation product has not been
identified [7]. It is thought that this receptor subtype may be important
during development, or may serve a unique function relative to the other
receptor subtypes [7]. No selective agonist has been described [7].
MUSCRINICM5R is an 8-element fingerprint that provides a signature for the
muscarinic M5 receptors. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the M5 receptors but distinguish them
from the rest of the muscarinic receptor family - motif 1 lies at the N-
terminus; motifs 2-7 span the third cytoplasmic loop; and motif 8 lies
at the C-terminus. A single iteration on OWL28.0 was required to reach
convergence, no further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
ACM5_HUMAN ACM5_MACMU ACM5_RAT
PROTEIN TITLES
ACM5_HUMAN MUSCARINIC ACETYLCHOLINE RECEPTOR M5 - HOMO SAPIENS (HUMAN).
ACM5_MACMU MUSCARINIC ACETYLCHOLINE RECEPTOR M5 - MACACA MULATTA (RHESU
ACM5_RAT MUSCARINIC ACETYLCHOLINE RECEPTOR M5 - RATTUS NORVEGICUS (RA
SCAN HISTORY
OWL28_0 1 50 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
MUSCRINICM5R1 Length of motif = 17 Motif number = 1
Muscarinic M5 receptor motif I - 1
PCODE ST INT
TVNGTPVNHQPLERHRL ACM5_HUMAN 11 11
TVNGTPVNHQALERHGL ACM5_RAT 10 10
MUSCRINICM5R2 Length of motif = 19 Motif number = 2
Muscarinic M5 receptor motif II - 1
PCODE ST INT
CPRPTLAQRERNQASWSSS ACM5_HUMAN 256 228
CPRPSLAQRERNQASWSSS ACM5_RAT 255 228
MUSCRINICM5R3 Length of motif = 19 Motif number = 3
Muscarinic M5 receptor motif III - 1
PCODE ST INT
WAKAEQLTTCSSYPSSEDE ACM5_HUMAN 294 19
WEKAEQVTTCSSYPSSEDE ACM5_RAT 293 19
MUSCRINICM5R4 Length of motif = 21 Motif number = 4
Muscarinic M5 receptor motif IV - 1
PCODE ST INT
KPATDPVLQVVYKSQGKESPG ACM5_HUMAN 314 1
KPTTDPVFQMVYKSEAKESPG ACM5_RAT 313 1
MUSCRINICM5R5 Length of motif = 19 Motif number = 5
Muscarinic M5 receptor motif V - 1
PCODE ST INT
SDYDTPNYLLSPAAAHRPK ACM5_HUMAN 354 19
SDYDTPKYFLSPAAAHRLK ACM5_RAT 353 19
MUSCRINICM5R6 Length of motif = 19 Motif number = 6
Muscarinic M5 receptor motif VI - 1
PCODE ST INT
KCVAYKFRLVVKADGNQET ACM5_HUMAN 375 2
KCVAYKFRLVVKADGTQET ACM5_RAT 374 2
MUSCRINICM5R7 Length of motif = 18 Motif number = 7
Muscarinic M5 receptor motif VII - 1
PCODE ST INT
GCHKVKIMPCPFPVAKEP ACM5_HUMAN 396 2
GCRKVKIMPCSFPVSKDP ACM5_RAT 395 2
MUSCRINICM5R8 Length of motif = 15 Motif number = 8
Muscarinic M5 receptor motif VIII - 1
PCODE ST INT
KKVEEKLYWQGNSKL ACM5_HUMAN 517 103
KKVEEKLYWQGNSKL ACM5_RAT 516 103
FINAL MOTIF SETS
MUSCRINICM5R1 Length of motif = 17 Motif number = 1
Muscarinic M5 receptor motif I - 2
PCODE ST INT
TVNGTPVNHQPLERHRL ACM5_HUMAN 11 11
TVNGTPVYHQPLERHRL ACM5_MACMU 11 11
TVNGTPVNHQALERHGL ACM5_RAT 10 10
MUSCRINICM5R2 Length of motif = 19 Motif number = 2
Muscarinic M5 receptor motif II - 2
PCODE ST INT
CPRPTLAQRERNQASWSSS ACM5_HUMAN 256 228
CPRPTLAQRERNQTSWSSS ACM5_MACMU 256 228
CPRPSLAQRERNQASWSSS ACM5_RAT 255 228
MUSCRINICM5R3 Length of motif = 19 Motif number = 3
Muscarinic M5 receptor motif III - 2
PCODE ST INT
WAKAEQLTTCSSYPSSEDE ACM5_HUMAN 294 19
QAKAEQLTTCSSYPSSEDE ACM5_MACMU 294 19
WEKAEQVTTCSSYPSSEDE ACM5_RAT 293 19
MUSCRINICM5R4 Length of motif = 21 Motif number = 4
Muscarinic M5 receptor motif IV - 2
PCODE ST INT
KPATDPVLQVVYKSQGKESPG ACM5_HUMAN 314 1
KPATDPVLQVVYKSRGKESPG ACM5_MACMU 314 1
KPTTDPVFQMVYKSEAKESPG ACM5_RAT 313 1
MUSCRINICM5R5 Length of motif = 19 Motif number = 5
Muscarinic M5 receptor motif V - 2
PCODE ST INT
SDYDTPNYLLSPAAAHRPK ACM5_HUMAN 354 19
HDSDTPNYFLSPAAAHRPK ACM5_MACMU 354 19
SDYDTPKYFLSPAAAHRLK ACM5_RAT 353 19
MUSCRINICM5R6 Length of motif = 19 Motif number = 6
Muscarinic M5 receptor motif VI - 2
PCODE ST INT
KCVAYKFRLVVKADGNQET ACM5_HUMAN 375 2
KCVAYKFRLVVKADGTQEN ACM5_MACMU 375 2
KCVAYKFRLVVKADGTQET ACM5_RAT 374 2
MUSCRINICM5R7 Length of motif = 18 Motif number = 7
Muscarinic M5 receptor motif VII - 2
PCODE ST INT
GCHKVKIMPCPFPVAKEP ACM5_HUMAN 396 2
GCHKVKIMPCSFPVAKEP ACM5_MACMU 396 2
GCRKVKIMPCSFPVSKDP ACM5_RAT 395 2
MUSCRINICM5R8 Length of motif = 15 Motif number = 8
Muscarinic M5 receptor motif VIII - 2
PCODE ST INT
KKVEEKLYWQGNSKL ACM5_HUMAN 517 103
KKVEEKLYWQGNSKL ACM5_MACMU 517 103
KKVEEKLYWQGNSKL ACM5_RAT 516 103
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