WORKLIST ENTRIES (1):
MUSCRINICM3R View alignment View Structure Muscarinic M3 receptor signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00243 MUSCARINICR; PR00538 MUSCRINICM1R; PR00539 MUSCRINICM2R
PRINTS; PR00541 MUSCRINICM4R; PR00542 MUSCRINICM5R
INTERPRO; IPR001183
Creation date 01-JUN-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. KERLAVAGE, A.R., FRASER, C.M., CHUNG, F-Z. AND VENTER, J.C.
Molecular structure and evolution of adrenergic and cholinergic receptors.
PROTEINS 1 287-301 (1986).
7. WATSON, S. AND ARKINSTALL, S.
Acetylcholine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.7-18.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
The muscarinic acetylcholine receptors, present in the central nervous
system, spinal cord motoneurons and autonomic preganglia, modulate a
variety of physiological functions, including airway, eye and intestinal
smooth muscle contractions; heart rate; and glandular secretions. The
receptors mediate adenylate cyclase attenuation, calcium and potassium
channel activation, and phosphatidyl inositol turnover [6]. This diversity
may result from the occurrence of multiple receptor subtypes (of which 5
are currently known, designated M1 to M5), which have been classified
based on observed differences in ligand binding to receptors in membranes
from several tissues.
The M3 receptor is found in high levels in neuronal cells of the CNS [7];
its distribution largely overlaps with that of M1 and M4 subtypes. It is
also found in peripheral ganglia, exocrine glands, smooth muscle, vascular
endothelium, and in cell lines. No selective agonist has been described [7].
MUSCRINICM3R is a 7-element fingerprint that provides a signature for the
muscarinic M3 receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the M3 receptors but distinguish them
from the rest of the muscarinic receptor family - motif 1 lies at the N-
terminus; motifs 2-6 span the third cytoplasmic loop; and motif 7 lies
at the C-terminus. Two iterations on OWL28.0 were required to reach
convergence, at which point a true set comprising 6 sequences was
identified. A single partial match was also found, BOVMRM3SUB, an M3
receptor fragment.
An update on SPTR37_9f identified a true set of 5 sequences.
SUMMARY INFORMATION
5 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 5 5 5 5 5 5 5
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
ACM3_PIG ACM3_BOVIN ACM3_HUMAN ACM3_RAT
ACM3_CHICK
PROTEIN TITLES
ACM3_PIG MUSCARINIC ACETYLCHOLINE RECEPTOR M3 - SUS SCROFA (PIG).
ACM3_BOVIN MUSCARINIC ACETYLCHOLINE RECEPTOR M3 - BOS TAURUS (BOVINE).
ACM3_HUMAN MUSCARINIC ACETYLCHOLINE RECEPTOR M3 - HOMO SAPIENS (HUMAN).
ACM3_RAT MUSCARINIC ACETYLCHOLINE RECEPTOR M3 - RATTUS NORVEGICUS (RA
ACM3_CHICK MUSCARINIC ACETYLCHOLINE RECEPTOR M3 - GALLUS GALLUS (CHICKE
SCAN HISTORY
OWL28_0 2 50 NSINGLE
SPTR37_9f 2 6 NSINGLE
INITIAL MOTIF SETS
MUSCRINICM3R1 Length of motif = 20 Motif number = 1
Muscarinic M3 receptor motif I - 1
PCODE ST INT
PLFPNISSSWIHSPSDAGLP ACM3_HUMAN 11 11
PLFPNISSSWIHGPSDAGLP ACM3_PIG 11 11
PLFPNISSSWVHSPSEAGLP ACM3_RAT 11 11
PLFPNISSSWIHGPSDTGLP ACM3_BOVIN 11 11
MUSCRINICM3R2 Length of motif = 22 Motif number = 2
Muscarinic M3 receptor motif II - 1
PCODE ST INT
TEAETENFVHPTGSSRSCSSYE ACM3_HUMAN 273 242
TEAEAENFVHPTGSSRSCSSYE ACM3_PIG 273 242
TEAEAENFVHPTGSSRSCSSYE ACM3_RAT 272 241
TEAEAENFVHPTGSSRSCSSYE ACM3_BOVIN 273 242
MUSCRINICM3R3 Length of motif = 20 Motif number = 3
Muscarinic M3 receptor motif III - 1
PCODE ST INT
KRSNRRKYGRCHFWFTTKSW ACM3_HUMAN 301 6
KRSARRKYGRCHFWFTTKSW ACM3_PIG 301 6
KRSSRRKYGRCHFWFTTKSW ACM3_RAT 300 6
KRSARRKYGRCHFWFTTKSW ACM3_BOVIN 301 6
MUSCRINICM3R4 Length of motif = 20 Motif number = 4
Muscarinic M3 receptor motif IV - 1
PCODE ST INT
DIGSETRAIYSIVLKLPGHS ACM3_HUMAN 356 35
DIGSETRAIYSIVLKLPGHS ACM3_PIG 356 35
DIGSETRAIYSIVLKLPGHS ACM3_RAT 355 35
DIGSETRAIYSIVLKLPGHS ACM3_BOVIN 356 35
MUSCRINICM3R5 Length of motif = 19 Motif number = 5
Muscarinic M3 receptor motif V - 1
PCODE ST INT
FSKLPIQLESAVDTAKTSD ACM3_HUMAN 424 48
FSKLPIQLESAVDTAKASD ACM3_PIG 424 48
FTKLPIQLESAVDTGKTSD ACM3_RAT 423 48
FSKLPIQLESAVDTAKASD ACM3_BOVIN 424 48
MUSCRINICM3R6 Length of motif = 20 Motif number = 6
Muscarinic M3 receptor motif VI - 1
PCODE ST INT
ATLPLSFKEATLAKRFALKT ACM3_HUMAN 452 9
ATLPLSFKEATLAKRFALKT ACM3_PIG 452 9
ATLPLSFKEATLAKRFALKT ACM3_RAT 451 9
ATLPLSFKEATLAKRFALKT ACM3_BOVIN 452 9
MUSCRINICM3R7 Length of motif = 19 Motif number = 7
Muscarinic M3 receptor motif VII - 1
PCODE ST INT
RKQQYQQRQSVIFHKRAPE ACM3_HUMAN 569 97
RKQQYQQRQSVIFHKRVPE ACM3_PIG 569 97
RKQQYQQRQSVIFHKRVPE ACM3_RAT 568 97
RKQQYQQRQSVIFHKRVPE ACM3_BOVIN 569 97
FINAL MOTIF SETS
MUSCRINICM3R1 Length of motif = 20 Motif number = 1
Muscarinic M3 receptor motif I - 2
PCODE ST INT
PLFPNISSSWIHGPSDAGLP ACM3_PIG 11 11
PLFPNISSSWIHGPSDTGLP ACM3_BOVIN 11 11
PLFPNISSSWIHSPSDAGLP ACM3_HUMAN 11 11
PLFPNISSSWVHSPSEAGLP ACM3_RAT 11 11
PLFSNVSSFWKRDSHGPGLL ACM3_CHICK 50 50
MUSCRINICM3R2 Length of motif = 22 Motif number = 2
Muscarinic M3 receptor motif II - 2
PCODE ST INT
TEAEAENFVHPTGSSRSCSSYE ACM3_PIG 273 242
TEAEAENFVHPTGSSRSCSSYE ACM3_BOVIN 273 242
TEAETENFVHPTGSSRSCSSYE ACM3_HUMAN 273 242
TEAEAENFVHPTGSSRSCSSYE ACM3_RAT 272 241
SEAETARFVHQTGSSRSLSSYE ACM3_CHICK 321 251
MUSCRINICM3R3 Length of motif = 20 Motif number = 3
Muscarinic M3 receptor motif III - 2
PCODE ST INT
KRSARRKYGRCHFWFTTKSW ACM3_PIG 301 6
KRSARRKYGRCHFWFTTKSW ACM3_BOVIN 301 6
KRSNRRKYGRCHFWFTTKSW ACM3_HUMAN 301 6
KRSSRRKYGRCHFWFTTKSW ACM3_RAT 300 6
KRSSRRKYRRCHFWLTMKSW ACM3_CHICK 349 6
MUSCRINICM3R4 Length of motif = 20 Motif number = 4
Muscarinic M3 receptor motif IV - 2
PCODE ST INT
DIGSETRAIYSIVLKLPGHS ACM3_PIG 356 35
DIGSETRAIYSIVLKLPGHS ACM3_BOVIN 356 35
DIGSETRAIYSIVLKLPGHS ACM3_HUMAN 356 35
DIGSETRAIYSIVLKLPGHS ACM3_RAT 355 35
DITAETRAIYSIVLKLPGHS ACM3_CHICK 404 35
MUSCRINICM3R5 Length of motif = 19 Motif number = 5
Muscarinic M3 receptor motif V - 2
PCODE ST INT
FSKLPIQLESAVDTAKASD ACM3_PIG 424 48
FSKLPIQLESAVDTAKASD ACM3_BOVIN 424 48
FSKLPIQLESAVDTAKTSD ACM3_HUMAN 424 48
FTKLPIQLESAVDTGKTSD ACM3_RAT 423 48
FSKLPIQPGSAETATASDG ACM3_CHICK 475 51
MUSCRINICM3R6 Length of motif = 20 Motif number = 6
Muscarinic M3 receptor motif VI - 2
PCODE ST INT
ATLPLSFKEATLAKRFALKT ACM3_PIG 452 9
ATLPLSFKEATLAKRFALKT ACM3_BOVIN 452 9
ATLPLSFKEATLAKRFALKT ACM3_HUMAN 452 9
ATLPLSFKEATLAKRFALKT ACM3_RAT 451 9
AALPLSFKEATLAKKFALKT ACM3_CHICK 502 8
MUSCRINICM3R7 Length of motif = 19 Motif number = 7
Muscarinic M3 receptor motif VII - 2
PCODE ST INT
RKQQYQQRQSVIFHKRVPE ACM3_PIG 569 97
RKQQYQQRQSVIFHKRVPE ACM3_BOVIN 569 97
RKQQYQQRQSVIFHKRAPE ACM3_HUMAN 569 97
RKQQYQQRQSVIFHKRVPE ACM3_RAT 568 97
RKQQYQQRQSVIFHKRIPR ACM3_CHICK 618 96
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