WORKLIST ENTRIES (1):
MUSCRINICM1R View alignment View Structure Muscarinic M1 receptor signature
Type of fingerprint: COMPOUND with 6 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00243 MUSCARINICR; PR00539 MUSCRINICM2R; PR00540 MUSCRINICM3R
PRINTS; PR00541 MUSCRINICM4R; PR00542 MUSCRINICM5R
INTERPRO; IPR002228
Creation date 01-JUN-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. KERLAVAGE, A.R., FRASER, C.M., CHUNG, F-Z. AND VENTER, J.C.
Molecular structure and evolution of adrenergic and cholinergic receptors.
PROTEINS 1 287-301 (1986).
7. WATSON, S. AND ARKINSTALL, S.
Acetylcholine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.7-18.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
The muscarinic acetylcholine receptors, present in the central nervous
system, spinal cord motoneurons and autonomic preganglia, modulate a
variety of physiological functions, including airway, eye and intestinal
smooth muscle contractions; heart rate; and glandular secretions. The
receptors mediate adenylate cyclase attenuation, calcium and potassium
channel activation, and phosphatidyl inositol turnover [6]. This diversity
may result from the occurrence of multiple receptor subtypes (of which 5
are currently known, designated M1 to M5), which have been classified
based on observed differences in ligand binding to receptors in membranes
from several tissues.
The M1 receptor is found in high levels in neuronal cells of the CNS; it
is particularly abundant in the cerebral cortex and hippocampus [7]. Its
distribution largely overlaps with that of M3 and M4 subtypes. In the
periphery, M1 receptors are found in autonomic ganglia and certain
secretory glands, and they are also found in cell lines. No truly
selective agonist has been described [7].
MUSCRINICM1R is a 6-element fingerprint that provides a signature for the
muscarinic M1 receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the M1 receptors but distinguish them
from the rest of the muscarinic receptor family - motif 1 lies at the N-
terminus; motifs 2-5 span the third cytoplasmic loop; and motif 6 lies
at the C-terminus. A single iteration on OWL28.0 was required to reach
convergence, no further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 5 sequences.
SUMMARY INFORMATION
5 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
6| 5 5 5 5 5 5
5| 0 0 0 0 0 0
4| 0 0 0 0 0 0
3| 0 0 0 0 0 0
2| 0 0 0 0 0 0
--+-------------------------------
| 1 2 3 4 5 6
True positives..
ACM1_HUMAN ACM1_MOUSE ACM1_RAT ACM1_MACMU
ACM1_PIG
PROTEIN TITLES
ACM1_HUMAN MUSCARINIC ACETYLCHOLINE RECEPTOR M1 - HOMO SAPIENS (HUMAN).
ACM1_MOUSE MUSCARINIC ACETYLCHOLINE RECEPTOR M1 - MUS MUSCULUS (MOUSE).
ACM1_RAT MUSCARINIC ACETYLCHOLINE RECEPTOR M1 - RATTUS NORVEGICUS (RA
ACM1_MACMU MUSCARINIC ACETYLCHOLINE RECEPTOR M1 - MACACA MULATTA (RHESU
ACM1_PIG MUSCARINIC ACETYLCHOLINE RECEPTOR M1 (BRAIN) - SUS SCROFA (P
SCAN HISTORY
OWL28_0 1 20 NSINGLE
SPTR37_9f 2 6 NSINGLE
INITIAL MOTIF SETS
MUSCRINICM1R1 Length of motif = 17 Motif number = 1
Muscarinic M1 receptor motif I - 1
PCODE ST INT
PPAVSPNITVLAPGKGP ACM1_HUMAN 6 6
PPAVSPNITVLAPGKGP ACM1_MOUSE 6 6
PPAVSPNITVLAPGKGP ACM1_PIG 6 6
PPAVSPNITVLAPGKGP ACM1_RAT 6 6
MUSCRINICM1R2 Length of motif = 20 Motif number = 2
Muscarinic M1 receptor motif II - 1
PCODE ST INT
RCCRCCRAPRLLQAYSWKEE ACM1_HUMAN 258 235
RCCRCCRAPRLLQAYSWKEE ACM1_MOUSE 258 235
RCCRCCRAPRLLQAYSWKEE ACM1_PIG 258 235
RCCRCCRAPRLLQAYSWKEE ACM1_RAT 258 235
MUSCRINICM1R3 Length of motif = 19 Motif number = 3
Muscarinic M1 receptor motif III - 1
PCODE ST INT
DEGSMESLTSSEGEEPGSE ACM1_HUMAN 281 3
DEGSMESLTSSEGEEPGSE ACM1_MOUSE 281 3
DEGSMESLTSSEGEEPGSE ACM1_PIG 281 3
DEGSMESLTSSEGEEPGSE ACM1_RAT 281 3
MUSCRINICM1R4 Length of motif = 18 Motif number = 4
Muscarininc M1 receptor motif IV - 1
PCODE ST INT
KMPMVDPEAQAPTKQPPR ACM1_HUMAN 303 3
KMPMVDPEAQAPTKQPPK ACM1_MOUSE 303 3
KMPMVDPEAQAPAKQPPR ACM1_PIG 303 3
KMPMVDSEAQAPTKQPPK ACM1_RAT 303 3
MUSCRINICM1R5 Length of motif = 17 Motif number = 5
Muscarinic M1 receptor motif V - 1
PCODE ST INT
GRDRAGKGQKPRGKEQL ACM1_HUMAN 333 12
GRDRGGKGQKPRGKEQL ACM1_MOUSE 333 12
GRERAGKGQKPRGKEQL ACM1_PIG 333 12
GRDRGGKGQKPRGKEQL ACM1_RAT 333 12
MUSCRINICM1R6 Length of motif = 18 Motif number = 6
Muscarinic M1 receptor motif VI - 1
PCODE ST INT
RWRKIPKRPGSVHRTPSR ACM1_HUMAN 441 91
RWRKIPKRPGSVHRTPSR ACM1_MOUSE 441 91
RWRKIPKRPGSVHRTPSR ACM1_PIG 441 91
RWRKIPKRPGSVHRTPSR ACM1_RAT 441 91
FINAL MOTIF SETS
MUSCRINICM1R1 Length of motif = 17 Motif number = 1
Muscarinic M1 receptor motif I - 2
PCODE ST INT
PPAVSPNITVLAPGKGP ACM1_HUMAN 6 6
PPAVSPNITVLAPGKGP ACM1_MOUSE 6 6
PPAVSPNITVLAPGKGP ACM1_RAT 6 6
PPAVSPNITVLAPGKGP ACM1_MACMU 6 6
PPAVSPNITVLAPGKGP ACM1_PIG 6 6
MUSCRINICM1R2 Length of motif = 20 Motif number = 2
Muscarinic M1 receptor motif II - 2
PCODE ST INT
RCCRCCRAPRLLQAYSWKEE ACM1_HUMAN 258 235
RCCRCCRAPRLLQAYSWKEE ACM1_MOUSE 258 235
RCCRCCRAPRLLQAYSWKEE ACM1_RAT 258 235
RCCRCCRPPRLLQAYSWKED ACM1_MACMU 258 235
RCCRCCRAPRLLQAYSWKEE ACM1_PIG 258 235
MUSCRINICM1R3 Length of motif = 19 Motif number = 3
Muscarinic M1 receptor motif III - 2
PCODE ST INT
DEGSMESLTSSEGEEPGSE ACM1_HUMAN 281 3
DEGSMESLTSSEGEEPGSE ACM1_MOUSE 281 3
DEGSMESLTSSEGEEPGSE ACM1_RAT 281 3
DEGSMESLTSSEGEEPGSE ACM1_MACMU 281 3
DEGSMESLTSSEGEEPGSE ACM1_PIG 281 3
MUSCRINICM1R4 Length of motif = 18 Motif number = 4
Muscarinic M1 receptor motif IV - 2
PCODE ST INT
KMPMVDPEAQAPTKQPPR ACM1_HUMAN 303 3
KMPMVDPEAQAPTKQPPK ACM1_MOUSE 303 3
KMPMVDSEAQAPTKQPPK ACM1_RAT 303 3
KMPMVDPEAQAPTKQPPR ACM1_MACMU 303 3
KMPMVDPEAQAPAKQPPR ACM1_PIG 303 3
MUSCRINICM1R5 Length of motif = 17 Motif number = 5
Muscarinic M1 receptor motif V - 2
PCODE ST INT
GRDRAGKGQKPRGKEQL ACM1_HUMAN 333 12
GRDRGGKGQKPRGKEQL ACM1_MOUSE 333 12
GRDRGGKGQKPRGKEQL ACM1_RAT 333 12
GRDRAGKGQKPRGKEQL ACM1_MACMU 333 12
GRERAGKGQKPRGKEQL ACM1_PIG 333 12
MUSCRINICM1R6 Length of motif = 18 Motif number = 6
Muscarinic M1 receptor motif VI - 2
PCODE ST INT
RWRKIPKRPGSVHRTPSR ACM1_HUMAN 441 91
RWRKIPKRPGSVHRTPSR ACM1_MOUSE 441 91
RWRKIPKRPGSVHRTPSR ACM1_RAT 441 91
RWRKIPKRPGSVHRTPSR ACM1_MACMU 441 91
RWRKIPKRPGSVHRTPSR ACM1_PIG 441 91
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