WORKLIST ENTRIES (1):
MARCKS View alignment MARCKS family signature
Type of fingerprint: COMPOUND with 4 elements
Links:
INTERPRO; IPR002101
PROSITE; PS00826 MARCKS_1; PS00827 MARCKS_2
Creation date 13-AUG-1998; UPDATE 07-JUN-1999
1. BLACKSHEAR, P.J.
The MARCKS family of cellular protein kinase C substrates.
J.BIOL.CHEM. 268 1501-1504 (1993).
2. THELEN, M., ROSEN, A., NAIRN, A.C. AND ADEREM, A.
Regulation by phosphorylation of reversible association of a myristoylated
protein kinase C substrate with the plasma membrane.
NATURE 351 320-322 (1991).
3. HARTWIG, J.H., THELEN, M., ROSEN, A., JANMEY, P.A., NAIRN, A.C.
AND ADEREM, A.
MARCKS is an actin filament crosslinking protein regulated by protein
kinase C and calcium-calmodulin.
NATURE 356 618-622 (1992).
4. UMEKAGE, T. AND KATO, K.
A mouse brain cDNA encodes a novel protein with the protein kinase C
phosphorylation site domain common to MARCKS.
FEBS LETT. 286 147-151 (1991).
Myristoylated alanine-rich C-kinase substrate (MARCKS) is a predominent
cellular substrate for protein kinase C (PKC) that has been implicated in
macrophage activation, neuro-secretion and growth factor-dependent
mitogenesis [1-3]. The N-terminal glycine is the site of myristoylation,
which allows effective binding of the protein to the plasma membrane, where
it co-localises with PKC [2]. MARCKS binds calmodulin in a calcium-dependent
manner; the region responsible for calcium-binding is highly basic, a domain
of 25 amino acids known as the PSD domain, which also contains the PKC
phosphorylation sites. When not phosphorylated, the PSD domain can bind
to filamentous actin [3]. It is believed that MARCKS may be a regulated
crossbridge between actin and the plasma membrane; modulation of the actin
cross-linking activity by calmodulin and phosphorylation, represent a
potential convergence of the calcium-calmodulin and PKC signal transduction
pathways in regulation of the actin cytoskeleton [3].
MARCKS-related protein (MRP) is similar to MARCKS in terms of properties
such as its myristoylation, phosphorylation and calmodulin-binding, and
shares a high degree of sequence similarity. Two regions that show highest
similarity are the kinase C phosphorylation site domain and the N-terminal
region containing the myristoylation site [4]. MARCKS and MRP amino acid
compositions are similar, but the alanine content of the latter is lower [4].
MARCKS is a 4-element fingerprint that provides a signature for the
myristoylated alanine-rich C-kinase substrate proteins. The fingerprint
was derived from an initial alignment of 5 sequences: the motifs were
drawn from short conserved regions spanning the full alignment length,
motifs 2 and 3 including the regions encoded by PROSITE patterns MARCKS_1
(PS00826) and MARCKS_2 (PS00827), the latter corresponding to the PSD domain.
Two iterations on OWL30.2 were required to reach convergence, at which point
a true set comprising 10 sequences was identified.
An update on SPTR37_9f identified a true set of 9 sequences.
SUMMARY INFORMATION
9 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 9 9 9 9
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
MACS_BOVIN MACS_HUMAN MACS_MOUSE MACS_RAT
MACS_CHICK O93503 MRP_HUMAN MRP_RABIT
MRP_MOUSE
PROTEIN TITLES
MACS_BOVIN MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE (MARCKS) (ACAM
MACS_HUMAN MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE (MARCKS) (PROT
MACS_MOUSE MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE (MARCKS) - MUS
MACS_RAT MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE (MARCKS) - RAT
MACS_CHICK MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE (MARCKS) - GAL
O93503 MYRISTOYLATED ALANINE-RICH C KINASE SUBSTRATE - XENOPUS LAEV
MRP_HUMAN MARCKS-RELATED PROTEIN (MAC-MARCKS) - HOMO SAPIENS (HUMAN).
MRP_RABIT MARCKS-RELATED PROTEIN (MAC-MARCKS) - ORYCTOLAGUS CUNICULUS
MRP_MOUSE MARCKS-RELATED PROTEIN (MAC-MARCKS) (BRAIN PROTEIN F52) - MU
SCAN HISTORY
OWL30_2 2 100 NSINGLE
SPTR37_9f 2 10 NSINGLE
INITIAL MOTIF SETS
MARCKS1 Length of motif = 12 Motif number = 1
MARCKS family motif I - 1
PCODE ST INT
SKTAAKGEATAE MACS_BOVIN 5 5
SKTAAKGEAAAE MACS_CHICK 5 5
SKTAAKGEAAAE MACS_HUMAN 5 5
SSKAPRGDVTAE MRP_MOUSE 4 4
SSKAPRGDVTAE MRP_RABIT 4 4
MARCKS2 Length of motif = 21 Motif number = 2
MARCKS family motif II - 1
PCODE ST INT
SPSKANGQENGHVKVNGDASP MACS_BOVIN 26 9
SPSKANGQENGHVKVNGDASP MACS_CHICK 25 8
SPSKANGQENGHVKVNGDASP MACS_HUMAN 26 9
SPAKANGQENGHVRSNGDLTP MRP_MOUSE 21 5
SPAKANGQENGHVKSNGDLTP MRP_RABIT 21 5
MARCKS3 Length of motif = 22 Motif number = 3
MARCKS family motif III - 1
PCODE ST INT
KRFSFKKSFKLSGFSFKKNKKE MACS_BOVIN 154 107
KRFSFKKSFKLSGFSFKKNKKE MACS_CHICK 120 74
KRFSFKKSFKLSGFSFKKNKKE MACS_HUMAN 155 108
KKFSFKKPFKLSGLSFKRNRKE MRP_MOUSE 89 47
KKFSFKKPFKLSGLSFKRNRKE MRP_RABIT 89 47
MARCKS4 Length of motif = 12 Motif number = 4
MARCKS family motif IV - 1
PCODE ST INT
EAQPECSPEAPP MACS_BOVIN 315 139
EAPSESSPEGPA MACS_CHICK 265 123
EAQPECSPEAPP MACS_HUMAN 315 138
EPSTPSGPESGP MRP_MOUSE 179 68
EPSTPSGPESGP MRP_RABIT 178 67
FINAL MOTIF SETS
MARCKS1 Length of motif = 12 Motif number = 1
MARCKS family motif I - 2
PCODE ST INT
SKTAAKGEATAE MACS_BOVIN 5 5
SKTAAKGEAAAE MACS_HUMAN 5 5
SKTAAKGEATAE MACS_MOUSE 5 5
SKTAAKGEAAAE MACS_RAT 5 5
SKTAAKGEAAAE MACS_CHICK 5 5
SKTAAKGEAATA O93503 6 6
SSKAPRGDVTAE MRP_HUMAN 4 4
SSKAPRGDVTAE MRP_RABIT 4 4
SSKAPRGDVTAE MRP_MOUSE 4 4
MARCKS2 Length of motif = 21 Motif number = 2
MARCKS family motif II - 2
PCODE ST INT
SPSKANGQENGHVKVNGDASP MACS_BOVIN 26 9
SPSKANGQENGHVKVNGDASP MACS_HUMAN 26 9
SPSKANGQENGHVKVNGDASP MACS_MOUSE 26 9
SPSKANGQENGHVKVNGDASP MACS_RAT 26 9
SPSKANGQENGHVKVNGDASP MACS_CHICK 25 8
SPSKANGQENGHVKVNGDASP O93503 27 9
SPAKANGQENGHVKSNGDLSP MRP_HUMAN 21 5
SPAKANGQENGHVKSNGDLTP MRP_RABIT 21 5
SPAKANGQENGHVRSNGDLTP MRP_MOUSE 21 5
MARCKS3 Length of motif = 22 Motif number = 3
MARCKS family motif III - 2
PCODE ST INT
KRFSFKKSFKLSGFSFKKNKKE MACS_BOVIN 154 107
KRFSFKKSFKLSGFSFKKNKKE MACS_HUMAN 155 108
KRFSFKKSFKLSGFSFKKSKKE MACS_MOUSE 148 101
KRFSFKKSFKLSGFSFKKSKKE MACS_RAT 148 101
KRFSFKKSFKLSGFSFKKNKKE MACS_CHICK 120 74
KRFSFKKSFKLSGFSFKKNKKE O93503 129 81
KKFSFKKPFKLSGLSFKRNRKE MRP_HUMAN 89 47
KKFSFKKPFKLSGLSFKRNRKE MRP_RABIT 89 47
KKFSFKKPFKLSGLSFKRNRKE MRP_MOUSE 89 47
MARCKS4 Length of motif = 12 Motif number = 4
MARCKS family motif IV - 2
PCODE ST INT
EAQPECSPEAPP MACS_BOVIN 315 139
EAQPECSPEAPP MACS_HUMAN 315 138
EPQPECSPEAPP MACS_MOUSE 292 122
EPQPECSPEAPP MACS_RAT 292 122
EAPSESSPEGPA MACS_CHICK 265 123
EATSESSPAADS O93503 274 123
EPSTPSGPESGP MRP_HUMAN 174 63
EPSTPSGPESGP MRP_RABIT 178 67
EPSTPSGPESGP MRP_MOUSE 179 68
User query: Display/Full Code "MARCKS"