WORKLIST ENTRIES (1):
LYSOZYMEG View alignment Lysozyme G signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36
PRINTS; PR00744 GLHYDRLASE37; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41
PRINTS; PR00747 GLHYDRLASE47; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52
PRINTS; PR00846 GLHYDRLASE56; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59
PRINTS; PR00748 MELIBIASE; PR00135 LYZLACT; PR00137 LYSOZYME
PRINTS; PR00684 T4LYSOZYME; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
INTERPRO; IPR002152
PDB; 153L 3Dinfo
SCOP; 153L
CATH; 153L
Creation date 04-JUN-1997; UPDATE 07-JUN-1999
1. WEAVER, L.H., GRUTTER, M.G. AND MATTHEWS, B.W.
The refined structures of goose lysozyme and its complex with a bound
trisaccharide show that the "goose-type" lysozymes lack a catalytic
aspartate residue.
J.MOL.BIOL. 245(1) 54-68 (1995).
2. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
Goose-type lysozyme (lysozyme G) hydrolyses 1,4-beta-linkages between
N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan hetero-
polymers of prokaryote cell walls. Lysozyme G shows preference for
N-acetylmuramic acid residues that are substituted with a peptide moiety;
it acts only as a glycanohydrolase.
The structure of goose egg-white lysozyme (GEWL) with a bound trisaccharide
has been refined to 1.6A resolution [1]. The trisaccharide occupies
analogous sites to the B, C and D subsites of chicken (HEWL) and phage T4
(T4L) lysozymes. All of these enzymes display the same characteristic
hydrogen bonding pattern between protein and substrate [1]. Glu73 of GEWL
corresponds closely to Glu35 of HEWL (Glu11 of T4L), supporting the view
that this group is critical to the catalytic mechanism. However, lysozyme G
has no obvious counterpart to Asp52 of chicken lysozyme (Asp20 in T4L),
suggesting that a second acidic residue is not essential for its catalytic
activity, and may not be required for the activity of other lysozymes.
The structure of GEWL belongs to the mainly alpha class, its sequence
showing no discernible similarity to other lysozymes. The enzyme has
been classified as belonging to family 23 of glycosyl hydrolases [2]
(http://expasy.hcuge.ch/cgi-bin/lists?glycosid.txt).
LYSOZYMEG is an 8-element fingerprint that provides a signature for
lysozyme G. The fingerprint was derived from an initial alignment of 4
sequences: the motifs were drawn from conserved regions spanning the
full alignment length - motif 1 spans the first 3 helices; motif 2 spans
helices 4 and 5; motif 3 encompasses part of helices 6 and 7; motif 4 spans
the C-terminus of helix 7 and the first beta-strand; motif 5 includes the
second beta-strand; motif 6 encompasses part of helices 8 and 9; motif 7
spans the C-terminus of helix 9 and helix 10; and motif 8 spans helices 11
and 12. A single iteration on OWL29.3 was required to reach convergence,
no further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 4 sequences.
SUMMARY INFORMATION
4 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 4 4 4 4 4 4 4 4
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
LYG_ANSAN LYG_CYGAT LYG_STRCA LYG_CHICK
PROTEIN TITLES
LYG_ANSAN LYSOZYME G (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMIDASE) (GOOS
LYG_CYGAT LYSOZYME G (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMIDASE) (GOOS
LYG_STRCA LYSOZYME G (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMIDASE) (GOOS
LYG_CHICK LYSOZYME G PRECURSOR (EC 3.2.1.17) (1,4-BETA-N-ACETYLMURAMID
SCAN HISTORY
OWL29_3 1 25 NSINGLE
SPTR37_9f 2 6 NSINGLE
INITIAL MOTIF SETS
LYSOZYMEG1 Length of motif = 21 Motif number = 1
Lysozyme G motif I - 1
PCODE ST INT
CYGNVNRIDTTGASCKTAKPE LYG_ANSAN 4 4
CYGNVNRIDTTGASCKTAKPE LYG_CYGAT 4 4
CYGSVSRIDTTGASCRTAKPE LYG_CHICK 30 30
CYGDVNRVDTTGASCKSAKPE LYG_STRCA 4 4
LYSOZYMEG2 Length of motif = 22 Motif number = 2
Lysozyme G motif II - 1
PCODE ST INT
YCGVSASKKIAERDLQAMDRYK LYG_ANSAN 28 3
YCGVPASKTIAERDLKAMDRYK LYG_CYGAT 28 3
YCGVRASRTIAERDLGSMNKYK LYG_CHICK 54 3
YCGVAASRKIAERDLQSMDRYK LYG_STRCA 28 3
LYSOZYMEG3 Length of motif = 19 Motif number = 3
Lysozyme G motif III - 1
PCODE ST INT
IKKVGEKLCVEPAVIAGII LYG_ANSAN 52 2
IKKVGEKLCVEPAVIAGII LYG_CYGAT 52 2
IKRVGEALCIEPAVIAGII LYG_CHICK 78 2
IKKVGQKLCVDPAVIAGII LYG_STRCA 52 2
LYSOZYMEG4 Length of motif = 21 Motif number = 4
Lysozyme G motif IV - 1
PCODE ST INT
SRESHAGKVLKNGWGDRGNGF LYG_ANSAN 71 0
SRESHAGKVLKNGWGDRGNGF LYG_CYGAT 71 0
SRESHAGKILKNGWGDRGNGF LYG_CHICK 97 0
SRESHAGKALRNGWGDNGNGF LYG_STRCA 71 0
LYSOZYMEG5 Length of motif = 19 Motif number = 5
Lysozyme G motif V - 1
PCODE ST INT
GLMQVDKRSHKPQGTWNGE LYG_ANSAN 92 0
GLMQVDKRSHKPQGTWNGE LYG_CYGAT 92 0
GLMQVDKRYHKIEGTWNGE LYG_CHICK 118 0
GLMQVDRRSHKPVGEWNGE LYG_STRCA 92 0
LYSOZYMEG6 Length of motif = 17 Motif number = 6
Lysozyme G motif VI - 1
PCODE ST INT
KTIQKKFPSWTKDQQLK LYG_ANSAN 125 14
KRIQKKFPSWTKDQQLK LYG_CYGAT 125 14
KKIQRKFPRWTRDQQLK LYG_CHICK 151 14
KAIQKKFPRWTKEQQLK LYG_STRCA 125 14
LYSOZYMEG7 Length of motif = 22 Motif number = 7
Lysozyme G motif VII - 1
PCODE ST INT
GGISAYNAGAGNVRSYARMDIG LYG_ANSAN 142 0
GGISAYNAGAGNVRSYARMDIG LYG_CYGAT 142 0
GGISAYNAGVGNVRSYERMDIG LYG_CHICK 168 0
GGISAYNAGPGNVRSYERMDIG LYG_STRCA 142 0
LYSOZYMEG8 Length of motif = 21 Motif number = 8
Lysozyme G motif VIII - 1
PCODE ST INT
TTHDDYANDVVARAQYYKQHG LYG_ANSAN 164 0
TTHDDYANDVVARAQYYKQHG LYG_CYGAT 164 0
TLHDDYSNDVVARAQYFKQHG LYG_CHICK 190 0
TTHDDYANDVVARAQYYKQHG LYG_STRCA 164 0
FINAL MOTIF SETS
LYSOZYMEG1 Length of motif = 21 Motif number = 1
Lysozyme G motif I - 2
PCODE ST INT
CYGNVNRIDTTGASCKTAKPE LYG_ANSAN 4 4
CYGNVNRIDTTGASCKTAKPE LYG_CYGAT 4 4
CYGDVNRVDTTGASCKSAKPE LYG_STRCA 4 4
CYGSVSRIDTTGASCRTAKPE LYG_CHICK 30 30
LYSOZYMEG2 Length of motif = 22 Motif number = 2
Lysozyme G motif II - 2
PCODE ST INT
YCGVSASKKIAERDLQAMDRYK LYG_ANSAN 28 3
YCGVPASKTIAERDLKAMDRYK LYG_CYGAT 28 3
YCGVAASRKIAERDLQSMDRYK LYG_STRCA 28 3
YCGVRASRTIAERDLGSMNKYK LYG_CHICK 54 3
LYSOZYMEG3 Length of motif = 19 Motif number = 3
Lysozyme G motif III - 2
PCODE ST INT
IKKVGEKLCVEPAVIAGII LYG_ANSAN 52 2
IKKVGEKLCVEPAVIAGII LYG_CYGAT 52 2
IKKVGQKLCVDPAVIAGII LYG_STRCA 52 2
IKRVGEALCIEPAVIAGII LYG_CHICK 78 2
LYSOZYMEG4 Length of motif = 21 Motif number = 4
Lysozyme G motif IV - 2
PCODE ST INT
SRESHAGKVLKNGWGDRGNGF LYG_ANSAN 71 0
SRESHAGKVLKNGWGDRGNGF LYG_CYGAT 71 0
SRESHAGKALRNGWGDNGNGF LYG_STRCA 71 0
SRESHAGKILKNGWGDRGNGF LYG_CHICK 97 0
LYSOZYMEG5 Length of motif = 19 Motif number = 5
Lysozyme G motif V - 2
PCODE ST INT
GLMQVDKRSHKPQGTWNGE LYG_ANSAN 92 0
GLMQVDKRSHKPQGTWNGE LYG_CYGAT 92 0
GLMQVDRRSHKPVGEWNGE LYG_STRCA 92 0
GLMQVDKRYHKIEGTWNGE LYG_CHICK 118 0
LYSOZYMEG6 Length of motif = 17 Motif number = 6
Lysozyme G motif VI - 2
PCODE ST INT
KTIQKKFPSWTKDQQLK LYG_ANSAN 125 14
KRIQKKFPSWTKDQQLK LYG_CYGAT 125 14
KAIQKKFPRWTKEQQLK LYG_STRCA 125 14
KKIQRKFPRWTRDQQLK LYG_CHICK 151 14
LYSOZYMEG7 Length of motif = 22 Motif number = 7
Lysozyme G motif VII - 2
PCODE ST INT
GGISAYNAGAGNVRSYARMDIG LYG_ANSAN 142 0
GGISAYNAGAGNVRSYARMDIG LYG_CYGAT 142 0
GGISAYNAGPGNVRSYERMDIG LYG_STRCA 142 0
GGISAYNAGVGNVRSYERMDIG LYG_CHICK 168 0
LYSOZYMEG8 Length of motif = 21 Motif number = 8
Lysozyme G motif VIII - 2
PCODE ST INT
TTHDDYANDVVARAQYYKQHG LYG_ANSAN 164 0
TTHDDYANDVVARAQYYKQHG LYG_CYGAT 164 0
TTHDDYANDVVARAQYYKQHG LYG_STRCA 164 0
TLHDDYSNDVVARAQYFKQHG LYG_CHICK 190 0
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